Child ; Hematuria ; diagnosis ; Humans

Objective To compare and analyze the clinical curative effect of pituitrin and oxytocin applying in laparoscopic uterine myomectomy intraoperation .Methods According to surgical hemostasis method ,68 patients with uterine fibroids were divided into pituitrin group (group A) and oxytocin group(group B).The surgery time,intr-aoperative blood loss ,the change of postoperative hemoglobin levels and the change of blood pressure before and after treatment were observed.Results In A group,the operative time was (69.8 ±18.0)min,blood loss was (68.3 ± 19.1)mL,postoperative discharge time was (15.2 ±7.8)h,preoperative and postoperative hemoglobin difference val-ue was (10.4 ±2.6) g/L,which were significantly better than those in group B [(82.8 ±17.6) min,(88.5 ± 25.6)mL,(19.4 ±5.9)h,(13.5 ±2.9)g/L],the differences were statistically significant (all P<0.05).Postopera-tive morbidity rate in group A was 5 cases,that in group B was 9 cases,the difference was not statistically significant (P>0.05).In A group,the systolic blood pressure was (152.0 ±8.5)mmHg after treatment for 15min,which was significantly higher than (132.5 ±10.2)mmHg in group B,the difference was statistically significant (P<0.05). There were no statistically significant differences in the systolic blood pressure after treatment for 30min and 45min, and the diastolic blood pressure before and after treatment (all P>0.05).Conclusion Application of pituitrin in laparoscopic surgical hemostasis for patients with uterine fibroids can effectively reduce the surgery time ,decrease the blood loss ,its surgical hemostasis effect is better than oxytocin ,which is worth further clinical application .

Brain metastases from lung cancer is a significant cause of morbidity and mortality,whichis lack of effective treatment.Because chemotherapeutic drugs are thought to not cross the blood-brain barrier(BBB), the role of chemotherapy in the treatment of brain metastases remains controversial. For yearsradiotherapy has been the standard treatment for brain metastases.However, recent studies have confirmedthat the BBB in patients with brain metastases tumors are broken down.At the same time,researchers havefound some new methods for chemotherapeutic drugs to reach the brain metastases.Chemotherapy will play amore and more active role in the treatment of brain metastases tumors.

OBJECTIVE: To evaluate the economic effects of different therapeutic schemes for knee osteoarthritis.METHODS: The clinical data of 116 patients with knee osteoarthritis were divided into three groups,namely,Group A(taking Celebrex 200 mg q.d for 28 days);Group B(Celebrex 200 mg daily orally plus topically application of Diclofenac diethylammonium);and Group C(Celebrex 200 mg daily orally plus topically application of 4 boxes of Capsacin ointment).The improvement of knee osteoarthritis was evaluated by Lysholm rating system.And a cost-effectiveness analysis was conducted.RESULTS: The costs of Group A,B,and C were 206.97 yuan,252.77 yuan and 298.53 yuan respectively;the effective rates were 61.54%,71.05% and 84.62%,respectively;the cost-effectiveness ratios were 336.32,355.76 and 352.79,respectively.The incremental cost-effectiveness ratios of Group B and C as against Group A were 481.60 and 396.71,respectively.CONCLUSION: In Group C compared Group A,the effective rate was significantly higher;and as compared with Group B,the cost for gaining each unit of effectiveness in Group C was significantly lower.Group C is the optimal one among the three schemes.

It has been shown that two pathways of signal transduction are activated under the stimulation of cytokines , such as IL-1?TNF-? ?EGF?TGF-? ?PDGF?IGF-1, secreted by fibroblast-like synoviocytes with rheumatoid arthritis. They are receptor tyrosine kinases-Ras-MAPK pathway and non- receptor tyrosine kinases-JAK-STAT pathway. The former activation plays an important role in chronic synovitis of rheumatoid arthritis. More researches are expected to focus on G-protein-AC-cAMP signal transduction pathway and the internal relations of all kinds of pathways.

Diagnosis, Differential ; Humans ; Incidence ; Lung ; pathology ; Lung Diseases, Interstitial ; epidemiology ; pathology ; therapy ; Prognosis ; Pulmonary Fibrosis ; epidemiology ; pathology ; therapy

Objective To study the effect of small interfering RNA(siRNA) of human papillomavirus(HPV) 18(E6) gene on apoptosis of HPV-related cervical HeLa cell line.Methods siRNA targeting HPV18 E6 mRNA was designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA sequence were then transfected into HeLa cells via Lipofectamine()~(TM)2000.Cell viability was determined by MTT assay.Apoptosis was detected by morphological observation and flow cytometry analysis.The expression level of HPV18 E6 mRNA was assayed by RT-PCR.Results The cell growth and viability of(siRNA) transfected group were significantly inhibited(P

Objective To explore the effect of sublingual immunotherapy (S-LIT) on the balance of Th17/Treg in children with mite allergic asthma. Methods All 124 cases with mite allergic asthma were randomly divided into two groups, the control group (62 cases) was treated with ladder type inhalation, and the treatment group (62 cases) was administered of dust mite drops on the basis of conventional treatment. The peripheral frequency of Th17 cell and Treg cell of all the subjects (before and after treatment) were detected by flow cytometry, the serum cytokine IL-10、IL-17 levels wer analyzed by ELISA. Results After 1 year treatment, the Th17 cells in treatment group (1.50 %±0.87 %) was significantly lower than that before treatment (3.39 % ± 1.58 %), and less than that of the control group after 1 year of treatment (2.42 %±1.32 %) (P <0.01);after 1 year treatment, the proportion of Treg cells in treatment group (4.05 %±1.36 %) was significantly higher than that before treatment (2.33 ± 0.81%), and more than that of the control group after 1 year of treatment (2.87 %± 0.87 %) (P <0.01). After 1 year treatment, the serum IL-10 levels in treatment group (64.76±27.79 pg/mL) was significantly higher than that before treatment (36.32 ± 11.53 pg/mL), and more than that of the control group after 1 years of treatment (50.32 ± 10.97 pg/mL) (P <0.01);after 1 year treatment, the serum IL-17 levels in treatment group (20.45±8.35 pg/mL) was significantly lower than that before treatment (86.48 ± 28.19 pg/mL), and significantly less than that of the control group after 1 year of treatment (46.32 ± 12.43 pg/mL) (P <0.01). After 1 year treatment, the Childhood Asthma Control Test (C-ACT)score in the treatment group (24.35 ± 8.47) was significantly higher than the control group (20.13 ± 6.86) (P <0.05). Conclusions SLIT can decrease airway inflammation and correct the imbalance of Th17/Treg in children with mite, allergic asthma.

Objective To investigate the expression of human defensin-alpha1-3 (HNP1-3) in breast cancer and in normal tissues and determine whether there exists a relationship between HNP1-3 expression and clinicopathologic features.Methods Immunohistochemical staining was used to detect the expression levels of HNP1-3 in 59 breast cancer specimens,and 20 normal tissues (fibroadenoma of breast or tumor-adjacent tissues).The relationship of the expression levels of HNP1-3 with patient’s age,clinical stage,histological grade,lymph node metastases and expression of PR/ER/C-erB-2 was analyzed.Results High level expression of HNP1-3 (95%) was expressed in those normal breast tissues.Different expression of HNP1-3 was found in breast cancer specimens and closely correlated with tumor histological stage and lymph node metastases (P

ObjectiveTo study streptococcus pneumonia capsular polysaccharide antibodies of patients with chronic obstructive pulmonary disease(COPD) in acute exacerbation and the feasibility of vaccination for patients with COPD.MethodsThe COPD in acute exacerbation without respiratory failure (RF)(COPD without RF group),COPD in acute exacerbation with RF (COPD with RF group),asthma (asthma group),healthy elderly examination(elderly examination group) and healthy youth examination (youth examination group) were selected,and each group had 15 cases.The levels of streptococcus pneumonia capsular polysaccharide antibodies IgG,IgM and IgA were measured in all groups.Results There was no significant difference in IgG among the five groups (P ＞ 0.05 ).The levels of IgM in COPD without RF group and elderly examination group were significantly lower than those in COPD with RF group,asthma group and youth examination group (0.554 ± 0.309 and 0.538 ± 0.327 vs.0.810 ± 0.387,0.887 ± 0.278 and 0.852 ± 0.305,P ＜ 0.05 ).Although the level of IgA in COPD without RF group was significantly lower than that in youth examination group(0.532 ±0.297 vs.0.930 ±0.502,P ＜0.05),there was no significant difference among the five groups.Conclusion Patients with COPD should inoculate pneumococcal vaccination.