Molecular imaging develops faster in the field of neuroimaging of ischemic stroke.Many studies have shown that its values in the aspects of cerebral blood flow monitoring during ischemic stroke and brain tissue metabolism.Ischemic penumbra is the basis of reperfusion therapy for acute ischemic stroke.The imaging methods,including magnetic resonance perfusion-weighted imaging/diffusion-weighted imaging mismatch,have been widely used to reveal ischemic penumbra because of their fast and strong availability.However,they can only reveal the blood flow of penumbra tissue.By contrast,molecular imaging technology can describe and measure the in vivo biological processes on the cellular and molecular levels,thus it can more accurately reveal the penumbral tissue.

Objective: To investigate the characteristics of extracranial internal carotid artery dissection (ICAD) and the application value of high-resolution MR imaging (HR-MRI) in the diagnosis and follow-up study. Methods: Four patients with extracranial ICAD were diagnosed by clinical and imaging and confirmed by 3D-HR-MRI. They received anticoagulant or antiplatelet therapies for 2 to 9 months and their clinical manifestations were followed up. Their prognostic evaluation was conducted using the National Institutes of Health Stroke Scale (NIHSS), and reexamined with 3D-HR-MRI for observing the changes of hematoma in the vascular walls. Results: Circled digit oneAll the 4 patients was detected by 3D-HR-MRI. The "double-lumen sign" and the "half-moon" shaped intramural hematoma with uniform strength and slightly higher signal could be seen on each axial sequence. Circled digit twoThe clinical prognosis of the 4 patients were good. Circled digit threeThe intramural hematomas were reexamined in 3 patients, their hematomas were significantly reduced or completely disappeared. One patient with true lumen thrombosis caused arterial occlusion had distal recanalization during the reexamination. Conclusion: HR-MRI may conducts the diagnosis and follow-up study of the extracranial ICAD. The prognosis of the extracranial ICAD may be good if it is diagnosed and treated promptly.

Uterine cervical prolapse concurrent with pregnancy is a rare situation. Large variety of management options for pregnancy, labor, and delivery to this situation had been reported. We have experienced one case of third-degree cervical prolapse combined with pregnancy. This woman visited our hospital in the late first trimester. We had managed the patient successfully with a vaginal pessary to maintain cervical placement. hereby, We report this case with a brief review.
Female ; Humans ; Pessaries ; Pregnancy Trimester, First ; Pregnancy* ; Prolapse*

In order to study the application of glial cell line-derived neurotrophic factor(GDNF)in clinic,gene mutation,fusion protein expression in E.coli and purification methods have been used to obtain the fragments of GDNF,GDNF(△N39),GDNF(△N39)-R9.Using primary cultured dopaminergic neurons and PC12 cells with transfected with GFR?1 and Ret to observe their biological function and cytotoxicity.Using B-Endo3 cells and Transwell method to analyze their delivery across the cellular membrane and blood brain barrier.The results show that GDNF(△N39)-R9 has the same neurotrophic function with wild GDNF and nearly no cytotoxicity to dopaminergic neurons and PC12-GFR?1-Ret cells and can get through effectually the cellular membrane and simulacrum of blood brain barrier with matrigel and B-Endo3.

[Objective]To explore the relationship between Pglycoprotein,survivin expression and chemotherapy outcome.[Method]The expressions of P-glycoprotein,and of survivin were examined by the immunohistochemistry in 38 specimens of preoperation biopsy of osteosarecomas.[Result]The response rate of the P-glycoprotein positive group was lower than that of P-glycoprotein negative group.The response rate of the survivin overexpression group was significant lower than survivin was found to be more valuable in predicting chemotherapy results.[Conclusion]The expressions of P-glycoprotein,and of survivin are clearly associated with osteosarcomas response to chemotherapy in osteosarecoma and survivin is a more valuable parameter.

Objective To summarize the clinical features and therapy of sarcoidosis in the South of China.Methods A retrospective analysis was perfomed in 31 patients with sarcoidosis in past 22 years.Results The most frequent age at onset of sarcoidosis was 30～50 years old.Female patients were more than that in male patients. X-ray test of chest was the major way to diagnose the disease at early stage.The typing of lymphocytes in broncho-alveolar lavage fluid(BALF) has valuable to diagnose,but confirmed diagnosis depended on biopsy of histopathology.Conclusions Glucocorticoid is effective drug treatment for the most the patients who are at stage Ⅰ and Ⅱ. Every patient should be treated individually .If necessary,we should add immunosuppressors.

Objective To explore the effect of persistent stress on the ultrastructure of cancellous bone in the process of fracture healing.Method A transverse osteotomy on both sides of the femoral tuberositas was performed in 20 adult dogs. One side was fixed with a self-designed tuberositas memofy-pressure connector (TMC) which was made of NiTi shape memory alloy, and the other with compressive steel-wire (SW) cerclage.Five animals in each group were sacrificed at 2, 4, 8, 12 weeks after operation and observed under transmission electron microscope.Results The fracture healing was accelerated in the TMC group.The osteoblasts, chondroblasts and collagen fibers at the fracture sites showed significantly different morphology between the 2 groups at every observation time.Most cells and collagen fibers in the TMC group grew along the same direction as the stress, but those in the SW group grew in disorder.Both the number and functional activity of the osteoblasts and chondroblasts were higher in the TMC group than in the SW group.Conclusion Persistent stress can enhance healing and bone reconstruction following a cancellous bone fracture.

The results of treatment of injuries of the thoracic and thoracolumbar spine with neurological involvement have been reviewed in a retrospective study of 14 patients who received surgical treatment. Seven patients were treated with open reduction and Harrington instrumentation and the others were treated with anterior fusion, laminectomy only, Roger wiring with H-graft and posterior fusion respectively. In the former group, distraction rods were used in all patients. Three patients with incomplete paraplegia and of the 4 patients with complete paraplegia, two patients showed marked neurological improvement. While, in the latter group, only two patients with incomplete paraplegia showed marked neurological improvement. Harrington instrumentation allowed easier postoperative nursing and early mobilization of the patients.
Dislocations ; Early Ambulation ; Humans ; Laminectomy ; Nursing ; Paraplegia ; Retrospective Studies ; Spine

OBJECTIVE:To study the inhibitory effects and mechanism of lutein on nasopharyngeal carcinoma C666-1 cells. METHODS:C666-1 cells were stimulated by lutein at different concentrations [0(blank control),20,40,80,160 mg/L] for dif-ferent time(0,12,24,48 h). The proliferation rate of cells was determined by CCK-8 assay,and apoptotic rate of cells was deter-mined by TUNEL method;Western blot was adopted to determine the phosphorylation of S6K and S6 proteins of AMPK and mTOR pathway. RESULTS:Compared with blank control group,proliferation rate of C666-1 cells was significantly reduced after treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 12,24,48 h(P<0.05). After treated with lutein(80,160 mg/L)for 48 h and lutein(160 mg/L)for 24,48 h,cell apoptosis was significantly increased(P<0.05). Lutein(80,160 mg/L) could promote intracellular AMPK phosphorylation,and inhibits mTOR pathway S6K,S6 protein phosphorylation after 48 h treat-ment (P<0.05). CONCLUSIONS:Lutein can inhibit nasopharyngeal carcinoma C666-1 cell proliferation and induce nasopharyn-geal carcinoma cell apoptosis and inhibit S6K,S6 protein phosphorylation through promoting AMPK phosphorylation.