Objective To investigate the caries prevalence of the first permanent molars among pupils in Zhongshan City in order to improve the level of oral health and to provide the direction and evidence for prevention and treatment strategies of caries.Methods Oral first permanent molars were examined among 1731 pupils aged from 7 to 12 years old in Zhongshan Experimental Primary School. The data were analyzed by SPSS 10 0 for Windows.Results The mean caries prevalent rate of first permanent molars was 32 62% among 1731 examined pupils with the mean DMFT of 0 48.Thefemales’meancariesprevalentrateandDMFToffirstpermanentmolarswerebothhigherthanmales’ .Theseven -yearoldgrouphadthelowest cariesprevalentrateandDMFTthanotheragegroups .Theocclusalsurfacewasthemostcommonsurfaceamongthecariesfirstpermanentmolarwith percentageof 73 5 2 .Conclusion Toprotectthefirstpermanentmolar ,weshouldreinforceoralhealtheducation ,regularexaminationoralhealth ,car ryingoutthefitandfissureseal,andfillingthedecayedteethintime .

Objective To investigate etiology of the 18 -45 years young ischemic stroke and risk factors .Methods We retro-spectively analyzed clinical data of 106 cases patients with ischemic stroke ,carotid ultrasound ,MRA ,CTA ,DSA results analyzed e-tiology ,type .compared patients and the same period youth for the medical check with risk factors .Results The proportion of LAA、SOE in low ages had significant differences with high age(P<0 .05) .The proportion of LAA was 29 .2% and the proportion of SUE was 25 .4% ,On hypertension ,hyperlipidemia ,drinking ,smoking ,obesity ,diabetes ,heart disease ,high homocystine ,young stroke group had significant differences with young control group (P<0 .05) .Conclusion Youth main cause of ischemic stroke is large artery atherosclerotic stroke and unknown cause of stroke .The main youth ischemic stroke risk factors are smoking ,hyperten-sion ,hyperlipidemia ,diabetes ,drinking ,heart disease .

The trace elements of Halibios were analysed and the effects of 5 kinds of Halibios on antiirritability, radical scavenging, memeory MAO-B activity, hemorheologic changes in animal were studied. The results showed that Halibios Acaudina molpadioides, Hippocampus Kuda ,Cavernularia habereri,Octopus valgaris ,and Octopus ocellatus contain abundant trace elements,most of them have effects on antiirritability, radical scavenging, rising memory and depressing MAO-B activity in animmal.

Objective To investigate the diagnostic value of using magnetic resonance imaging (MRI) and transabdominal sonography in antenatal placental adhesive disorders,to provide a theoretical basis for choosing different delivery ways.Methods 75 patients were assessed the placenta structure with MRI and transabdominal sonography.The patients were divided into two groups according placenta in the anterior and posterior uterus(41 cases in anterior group,34 cases in posterior group).Observed relationship between placenta and myometrium,compared imaging finding with pathological or clinical results.Results The sensitivity in diagnosis of placenta in the anterior uterine group was 75.0％ with MRI and 95.0％ for transabdominal Sonography (P＜ 0.05).The specificity was 90.1 ％ with MRI and 80.9 ％ for transabdominal sonography (P＞0.05).The sensitivity in diagnosis of placenta in the posterior uterine group was 95.8％ with MRI and 66.7 ％ for transabdominal Sonography(P＜0.05).The specificity was 90.0％ with MRI and 70.0％ for sonography (P＜0.05).There were significantly difference the imaging feature of uterine bulging,increased subplacental vascularity and dark intraplacental bands on T2-weighted images in placenta accrete group and nonplacenta accretegroup (P＞0.05).Conclusion Both sonography and MRI have fairly good sensitivity and imaging features for prenatal diagnosis of placenta accrete.Especially,MRI is an excellent tool for diagnosis of placenta accrete in the posterior uterine.

In this paper, Liuwei Dihuang pill was used to study the identification of Chinese patent medicine by fluorescence sequencing typing technology. The DNA of Paeonia suffruticosa was used as template to amplify by five pair of FAM fluorescence labeling primers. Then, the amplified products were sequenced. The sequencing results were analyzed by GeneMarker V1.80 to screen the best fluorescence labeling primers. As a result, psbA-trnH fluorescence labeling primer was used to identify the raw materials of Liuwei Dihuang pill. The results showed that three kinds of raw plant medicinal materials in Liuwei Dihuang pill were able to be correctly identified by psbA-trnH fluorescence labeling primer. The fluorescence sequencing typing technology can stably and accurately distinguish raw medicinal materials in Chinese patent medicine.
DNA Primers ; chemistry ; genetics ; DNA, Plant ; chemistry ; genetics ; Drugs, Chinese Herbal ; chemistry ; standards ; Fluorescent Dyes ; chemistry ; Plants, Medicinal ; chemistry ; genetics ; Polymerase Chain Reaction ; instrumentation ; methods ; Quality Control ; Staining and Labeling

C-Reactive Protein ; analysis ; Calcitonin ; blood ; Calcitonin Gene-Related Peptide ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Pneumonia ; blood ; diagnosis ; Protein Precursors ; blood

To assess methodological quality of clinical studies using Aidi Injection as an adjunct therapy for non-small cell lung cancer (NSCLC) and to evaluate the effects of Aidi Injection.

In order to obtain an efficient screening procedure for identification of succinate producing anaerobic strains,a semi-quantitative paper chromatography method was developed. Lactic acid and acetic acid were identified as the main byproducts in the process of succinate production by the high performance liquid chromatography (HPLC).Succinic acid was completely separated from the byproducts of lactic acid and acetic acid in the same broth developed by paper chromatography.The content of succinic acid was calculated by a semi-quantitative method.The results showed that paper chromatography was a simple and cost effective method that could be utilized to screen anaerobic strains producing succinic acid.

Objective To analyze the gene mutation and clinical characteristics of BRCA 1/2 by high resolution melting curve ( HRM ) in breast cancer patients and high risk groups , and to discuss the application value for BRCA 1/2 mutation detection by using HRM curve analysis in people at high risk of breast cancer.Method The clinical control analysis was used ,Peripheral blood samples of 52breast cancer patients,their first-degree relatives (52 cases consisting of high risk group ) and 40 healthy people without family history of breast cancer cases were collected from Anhui Province Tumor Hospital or Bozhou People ′s Hospital during March 2015 to June 2016.To establish an effective method for BRCA 1/2 mutation detection by using HRM curve, and the mutation positive results were validated by gene sequencing .To analyze the correlation between the of BRCA 1/2 gene mutation and the risk factors , a logistic method was used .Results 9 cases of BRCA1/2 gene mutations were found in 52cases of breast cancer patients and the mutation rate was 17.3%.5 cases of BRCA1/2 gene mutations were found in 52 cases of breast cancer high risk groups and the mutation rate was 9.6%.In 40 healthy people who without family history of breast cancer cases ,we found only 1 gene mutation case.All the mutation positive results detected by HRM curve analysis were matched with gene sequencing results .BRCA1/2 gene mutations and the risk factors such as primiparous age and chronic mammary gland disease have a certain correlation .In the 9 cases of BRCA1/2 gene mutations , we found 5 cases of BRCA1/2 gene mutations in their first-degree relatives, with the consistency of 44.4%(4/9).Conclusion Thebreast cancer patients′s first-degree relatives have a high mutation rates on BRCA1/2 gene and they can be the key screening objects .HRM curve analysis can be used in health screening and risk assessment at the breast cancerhigh risk groups .

An analytical method of on-line injection technique including on-line dilution and on-line preconcentration with inductively coupled plasma mass spectrometry ( ICP-MS ) was established for the determination of trace metals in seawater, such as Cr, Mo, Cu, Pb, Zn, Cd, Fe, Mn, Ni, V, Co. This method could automatically make standard curves and match matrix, and by the on-line dilution and preconcentration modes, 11 trace metals could be analyzed directly. With high-level automation, CASS-5 and NASS-6 seawater standard samples were analyzed accurately by this method. The recovery of metals in seawater sample addition was between 80% and 115%. The method quantitation limits (μg/L ) of theses metals in seawater were 0. 06(Cr), 0. 06(Mo), 0. 01(Cu), 0. 005(Pb), 0. 01(Zn), 0. 006(Cd), 0. 03 (Fe), 0. 02(Mn), 0. 01(Ni), 0. 01(V), and 0. 01 (Co), respectively. This method was applied to analyze seawater samples of various salinities in Zhejiang coast, and the analysis results were consistent with results of conventional atomic absorption method.