BACKGROUND: Both c-fosand heat-shock protein (HSP) 70 genes are closely relevant with and participate in the occurrence and development of blood stasis syndrome of primary hypertension. It is importance to study on their correlation for prevention of primary hypertension.OBJECTIVE: Immunohistochemical method was used to determine the expression of HSP70 and c-fos genes in blood stasis syndrome of primary hypertension so as to probe into their correlation.DESIGN: Case analysis.SETTING: Department of Traditional Chinese Medicine, Medical College of Jinan University.PARTICIPANTS: Totally 148 cases of primary hypertension were selected, diagnosed in ward and clinic of Department of Cardiac Vascular Internal Medicine of Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine from March 2002 to march 2003, of which, 80 cases were male and 48 cases were female.INTERVENTIONS: At 7:30 to 8:00 in the morning on the 3rd of hospitalization, 2 mL intravenous blood was collected in fasting for immunohistochemical determination of HSP70 and c-fos genes. Evaluative standards:negative expression (-), no brown-yellow stained cell appeared; positive expression (+), positive cell count of each high power field ＜ 20％; dominant expression (++), positive cell count of each high power field was in the range from 20％ to 50％; strong positive expression (+++), positive cell count of each high power field was ＞ 50％. Ridit analysis statistical method was used for the correlation analysis.MAIN OUTCOME MEASURES: Expression of HSP70 and c-fos genes.RESULTS: Totally 148 cases of primary hypertension participated in the experiment and the blood sample of all of those entered the result analysis.① Six cases presented positive expression of both HSP70 and c-fos genes.② Four cases presented dominant expression of HSP70 gene and positive expression of c-fos gene respectively. ③ Twelve cases presented strong positive expression of HSP70 gene and positive expression of c-fos gene respectively. ④ Six cases presented positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑤ Nineteen cases presented dominant expression of both HSP70 and c-fos genes. ⑥ Sixteen cases presented strong positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑦ One case presented positive expression of HSP70 gene and strong positive expression of c-fos gene respectively. ⑧ Twenty-eight cases presented dominant expression of HSP70 gene and strong positive expression of c-fos respectively. ⑨ Fifty-six cases presented strong positive expression of both HSP70 and c-fos genes. ⑩ The grade of positive expression presented significant correlation between HSP70 gene and c-fos gene (R =0.281 1-0.644 6, P＜0.01).CONCLUSION: In blood stasis syndrome of primary hypertension, the high expression presents in both HSP70 and c-fos genes, indicating remarkably correlation.

Clinical practice is an important link of medical education. The change of medical modes and conditions, the pressure of employment and entrance examination for graduates affect the quality of clinical practice. General hospitals have improved a series of management measures to strengthen and ensure the quality of clinical practice. Furthermore, we shound further explore the new mode of teaching to improve the quality of clinical medicine education.

Objective: To further probe into mechanism and essence of blood stasis from gene level. Methods: 160 cases of hypertension and type Ⅱ diabetes with blood stasis or no blood stasis were strictly selected according to criteria of diagnosis and by combination of diseases with syndrome, and expressions of c-fos, c-jun and CD62p genes were investigated, compared and analyzed with healthy persons as controls. Results: There were abnormal expressions of c-fos,c-jun and CD62p genes in the patient with blood stasis, and there were close relation of the abnormality and expression levels of the genes with various types of blood stasis. Conclusion: Genomics is the best way for studies of essence of blood stasis syndrome, which can enrich and develop basic theory of TCM and provide a basis of molecular biology for clinical therapeutics of TCM.

Cholestasis ; Humans ; Liver Diseases

With rapid social development rhythm, the incidence of gynecological diseases gradually rise. Traditional Chinese medicine has made irreplaceable position in the treatment of gynecological disease. Due to the characteristics of gynecological diseases, the syndrome differentiation and treatment has unique characteristics. In this paper, according to menstrual disease, leukorrheal diseases, pregnancy diseases, puerperal diseases and miscellaneous diseases in order, combining documents discussion and old doctors of traditional Chinese medicine treatment of gynecological disease experiences, illustrate the key points of drug selection in the treatment process. We can get a revelation that appropriate choice of tradition Chinese medicine is an indispensable part for healing effects. Through the analysis of characteristics of drugs, we can understand the feature of each period in disease of department of gynecology, increase the ability of usinig traditional Chinese medicine and improve the level of clinical treatment.
Female ; Genital Diseases, Female ; drug therapy ; Humans ; Leukorrhea ; drug therapy ; Medicine, Chinese Traditional ; methods ; Menstruation Disturbances ; drug therapy ; Practice Patterns, Physicians' ; Pregnancy ; Pregnancy Complications ; drug therapy ; Puerperal Disorders ; drug therapy ; Treatment Outcome

Embryonic diapauses mean that early embryos stop developing for some reasons in early pregnant stage. The embryo has dead in uterine cavity, but has not yet discharged. The early clinical manifestation in the areas of traditional Chinese medicine (TCM) can be classified as "pregnant vaginal bleeding", "fetal irritability" and other diseases. Embryonic diapause is a common and difficult gynecologic clinical disease. The prevalence rate which is increasing has become a major reproductive health problem. So the prevention research of embryonic diapauses is very significant. In this paper, through the induction and the summary of Chinese and Western medicine dynamic researches and control methods of embryonic diapauses, detailing a list of pathogenesis and treatment progress in embryonic diapauses. Besides, it can lay the foundation for further study and reducing embryonic diapauses incidence, which can promote reproductive health development.
Fetal Growth Retardation ; etiology ; metabolism ; prevention & control ; psychology ; Humans ; Medicine ; methods ; Medicine, Chinese Traditional ; methods ; Western World

Objective To discuss the disinfection effect of the wiping towel with microwave oven. Methods 200 wiping towels were chosen in class Ⅱ environment in our hospital. 100 wiping towels (the control group)were disinfected by soaking in the chlorine-containing disinfectant,the other (the observation group )by microwave. The disinfection effect, the disinfection time, and the economic cost in three months were compared between two groups. Results There was no significant difference between two groups in the disinfection effect. There was significant difference between two groups in the disinfection time,the economic cost in three months. Conclusions Adopting microwave oven for towel disinfeciton has reliable result and is convenient. It costs shorter time and lower cost. The new method has no chemical side effect and can replace traditional method. It is an ideal disinfection method and worthy of clinical application.

The funds of New Rural Cooperative Medical System, including the input of the state, the collective and personal, must be strictly managed and reasonably used, so as to implement the New Rural Medical System Policy well and contribute all the participated peasants. It analyzed the needs of improving the level of accounting personnel qualifications, account settings, original vouchers quality, price, charge management and medicine management process in Financial management of primary medical institutions, and put forward suggestions on improving the accounting training, access, medicine purchasing, selling and storage management, information management, information disclosure, supervision and inspection in financial management of primary medical institutions.

Objective To study the maturation of the postural equilibrium in young children and the effects of eye-closure.Methods Altogether 214 healthy children age 4~15 were examined for upright postural equilibrium using a computerized equilibrium instrument(ST-939).With 20 healthy adults as control group,the velocity of postural sway was measured.Results ①Within the age-range of the children tested,postural sway decreased linearly with age.②Eye-closure had a remarkably effect on children's sway velocity in comparison with adult values before age eight.Conclusion Children's postural stability gradually maturates with the age.Young children may use visual information to control balance in a manner different from that of adults.It is not until after eight-year old that adults-like balance-control strategies begin to appear.

Objective The liver is the main target of colon cancer metastasis, and liver metastasis is important and difficult in colon cancer therapy.This study was to investigate the synergistic effects of Honghua injection combined with oxaliplatin in treatment of liver metastasis from colon cancer. Methods Spleen-preserving method was used to establish the model of liver metastasis from colon cancer.The model mice were randomly divided into 5 groups:control group, oxaliplatin group, low dose ( low dose of Honghua injection combined with oxaliplatin) group, medium dose ( medium dose of Honghua injection combined with oxaliplatin) group, high dose (high dose of Honghua injection combined with oxaliplatin) group (n=10).Oxaliplatin were given twice a week at a dose of 5 mg/kg.Honghua injection was given at doses of 2.5, 1.25 and 0.625 g/kg for consecutive 14 days, and the control group received sa-line.After 14 d, all the mice were killed, and the tumor inhibiting rates and liver metastasis rates were calculated.The levels of serum vascular endothelial growth factor ( VEGF) and hepatocyte growth factor ( HGF) were measured by ELISA, and the mRNA expressions of VEGF and HGF in liver were determined by PCR. Results The inhibition rate of low, middle and high dose group achieved 63.2%, 69.2%and 71.4%respectively, which was significantly higher than that of control group (P<0.01).Compared with oxaliplatin group,middle and high dose groups showed a significant higher inhibition rate (P<0.05).Compared with control group, the liver metastasis rates in middle and high dose groups significantly decreased from 90%to 60%and 50%respectively (P<0.05).The serum VEGF and HGF levels showed significant decrease in all treatment groups([83.4 ±25.6],[81.5 ±23.7],[75.8 ±19.4],[72.7 ±20.5] pg/mL),([56.2 ±13.8],[55.4 ±15.7],[48.9 ±13.6],[42.3 ±11.8]pg/mL) compared with control group ([125.3 ±36.2], [98.4 ±26.1]pg/mL) (P<0.01).The mRNA expressions of VEGF and HGF in liver were significantly decreased in all the Hong-hua injection combined with oxaliplatin groups compared with control group ( P<0.05) .Compared with oxaliplatin group, the serum VEGF level in high dose group showed a significant reduced mRNA expression of VEGF (P<0.05). Conclusion Honghua injec-tion can significantly enhance the inhibitory effects of Oxaliplatin on liver metastasis from colon cancer, and the mechanism may con-tribute to the inhibition of VEGF expression and the prevention of angiogenesis in liver.