Objective: To further probe into mechanism and essence of blood stasis from gene level. Methods: 160 cases of hypertension and type Ⅱ diabetes with blood stasis or no blood stasis were strictly selected according to criteria of diagnosis and by combination of diseases with syndrome, and expressions of c-fos, c-jun and CD62p genes were investigated, compared and analyzed with healthy persons as controls. Results: There were abnormal expressions of c-fos,c-jun and CD62p genes in the patient with blood stasis, and there were close relation of the abnormality and expression levels of the genes with various types of blood stasis. Conclusion: Genomics is the best way for studies of essence of blood stasis syndrome, which can enrich and develop basic theory of TCM and provide a basis of molecular biology for clinical therapeutics of TCM.

Clinical practice is an important link of medical education. The change of medical modes and conditions, the pressure of employment and entrance examination for graduates affect the quality of clinical practice. General hospitals have improved a series of management measures to strengthen and ensure the quality of clinical practice. Furthermore, we shound further explore the new mode of teaching to improve the quality of clinical medicine education.

BACKGROUND: Both c-fosand heat-shock protein (HSP) 70 genes are closely relevant with and participate in the occurrence and development of blood stasis syndrome of primary hypertension. It is importance to study on their correlation for prevention of primary hypertension.OBJECTIVE: Immunohistochemical method was used to determine the expression of HSP70 and c-fos genes in blood stasis syndrome of primary hypertension so as to probe into their correlation.DESIGN: Case analysis.SETTING: Department of Traditional Chinese Medicine, Medical College of Jinan University.PARTICIPANTS: Totally 148 cases of primary hypertension were selected, diagnosed in ward and clinic of Department of Cardiac Vascular Internal Medicine of Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine from March 2002 to march 2003, of which, 80 cases were male and 48 cases were female.INTERVENTIONS: At 7:30 to 8:00 in the morning on the 3rd of hospitalization, 2 mL intravenous blood was collected in fasting for immunohistochemical determination of HSP70 and c-fos genes. Evaluative standards:negative expression (-), no brown-yellow stained cell appeared; positive expression (+), positive cell count of each high power field ＜ 20％; dominant expression (++), positive cell count of each high power field was in the range from 20％ to 50％; strong positive expression (+++), positive cell count of each high power field was ＞ 50％. Ridit analysis statistical method was used for the correlation analysis.MAIN OUTCOME MEASURES: Expression of HSP70 and c-fos genes.RESULTS: Totally 148 cases of primary hypertension participated in the experiment and the blood sample of all of those entered the result analysis.① Six cases presented positive expression of both HSP70 and c-fos genes.② Four cases presented dominant expression of HSP70 gene and positive expression of c-fos gene respectively. ③ Twelve cases presented strong positive expression of HSP70 gene and positive expression of c-fos gene respectively. ④ Six cases presented positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑤ Nineteen cases presented dominant expression of both HSP70 and c-fos genes. ⑥ Sixteen cases presented strong positive expression of HSP70 gene and dominant expression of c-fos gene respectively. ⑦ One case presented positive expression of HSP70 gene and strong positive expression of c-fos gene respectively. ⑧ Twenty-eight cases presented dominant expression of HSP70 gene and strong positive expression of c-fos respectively. ⑨ Fifty-six cases presented strong positive expression of both HSP70 and c-fos genes. ⑩ The grade of positive expression presented significant correlation between HSP70 gene and c-fos gene (R =0.281 1-0.644 6, P＜0.01).CONCLUSION: In blood stasis syndrome of primary hypertension, the high expression presents in both HSP70 and c-fos genes, indicating remarkably correlation.

With rapid social development rhythm, the incidence of gynecological diseases gradually rise. Traditional Chinese medicine has made irreplaceable position in the treatment of gynecological disease. Due to the characteristics of gynecological diseases, the syndrome differentiation and treatment has unique characteristics. In this paper, according to menstrual disease, leukorrheal diseases, pregnancy diseases, puerperal diseases and miscellaneous diseases in order, combining documents discussion and old doctors of traditional Chinese medicine treatment of gynecological disease experiences, illustrate the key points of drug selection in the treatment process. We can get a revelation that appropriate choice of tradition Chinese medicine is an indispensable part for healing effects. Through the analysis of characteristics of drugs, we can understand the feature of each period in disease of department of gynecology, increase the ability of usinig traditional Chinese medicine and improve the level of clinical treatment.
Female ; Genital Diseases, Female ; drug therapy ; Humans ; Leukorrhea ; drug therapy ; Medicine, Chinese Traditional ; methods ; Menstruation Disturbances ; drug therapy ; Practice Patterns, Physicians' ; Pregnancy ; Pregnancy Complications ; drug therapy ; Puerperal Disorders ; drug therapy ; Treatment Outcome

High quality of fertility and pregancy is a specific presentation of family planning in this new historic environment. Our country adopted two-child policy from November, 2013. This symbolized an adjustive stage of reproduction policy in 21st century. It is a major adjustment in the development of national population. As a contry of tremendous population, it is very important to have a high- quality fertility and pregancy for the future of the whole nation, which would improve population quality and restrict development of population. The morbidity of infertility has increased significantly, which brings a huge crisis for national population. Under the guidance of high-quality fertility and pregancy in Chinese medicine theory, improving fertile women's ability of high-quality fertility and pregancy, and follow-up studying is of vital importance for evaluating the treatment of abortion prevention by traditional Chinese medicine and human health of high-quality fertility and pregancy.
Adult ; Blood Circulation ; drug effects ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Follow-Up Studies ; Humans ; Infertility, Female ; drug therapy ; physiopathology ; Kidney ; drug effects ; physiopathology ; Medicine, Chinese Traditional ; Pregnancy ; Pregnancy Outcome ; Reproduction

Embryonic diapauses mean that early embryos stop developing for some reasons in early pregnant stage. The embryo has dead in uterine cavity, but has not yet discharged. The early clinical manifestation in the areas of traditional Chinese medicine (TCM) can be classified as "pregnant vaginal bleeding", "fetal irritability" and other diseases. Embryonic diapause is a common and difficult gynecologic clinical disease. The prevalence rate which is increasing has become a major reproductive health problem. So the prevention research of embryonic diapauses is very significant. In this paper, through the induction and the summary of Chinese and Western medicine dynamic researches and control methods of embryonic diapauses, detailing a list of pathogenesis and treatment progress in embryonic diapauses. Besides, it can lay the foundation for further study and reducing embryonic diapauses incidence, which can promote reproductive health development.
Fetal Growth Retardation ; etiology ; metabolism ; prevention & control ; psychology ; Humans ; Medicine ; methods ; Medicine, Chinese Traditional ; methods ; Western World

Cholestasis ; Humans ; Liver Diseases

Objective To study the effect of apoptosis signal regulating kinase 1(ASK1) protein expression in hypertrophic myocardium in rat models of hypertension.Methods The rat model of hypertension was established by abdominal aortic constriction.Six weeks later,the reactive oxygen species(ROS) level and ASK1 protein expression in myocardium of left ventricle were detected respectively by color matching and Western blotting.Results The left ventricular mass index,ROS level and ASK1 protein expression all increased significantly in hypertensive rats as compared with those underwent sham operation.Conclusion Under chronic pressure overload,the activated ASK1 with the increasing ROS may play an important role in signal transduction pathway of myocardial hypertrophy.

Objective To evaluate the feacibility of microalbumin(MA),alpha-1 -raicroglobulin (?1-MG) ,N-acetyl-beta-D-glucosaminidase (NAG), retinol-binding protein (RBP)as predictors of outcome in critically ill patients. Methods A prospective study was underwent in 30 critically ill patients.Urinary samples were collected at ICU admission and on the third,seventh ICU day for MA,?1-MG,NAG,RBP measurement.The severity of illness was assessed by APACHE II score calculated on the first ICU day,and the degree of organ dysfunction was assessed using SOFA score calculated on the first,third,seventh ICU day. Results MA,?1-MG,the durations of ICU stay and mechanical ventilation,APACHE II score, SOFA score were relative factors of MODS and death in ICU. There was significant association of APACHE II with MA (r=0.397),?1-MG (r=0.448), and RBP (r= 0.465), respectively. The area under ROC curve of APACHE II score,SOFA score,MA,?1-MG, RBP,NAG to predicate the death in ICU were 0.875 ( P

Objective To study the effect of small interfering RNA(siRNA) of human papillomavirus(HPV) 18(E6) gene on apoptosis of HPV-related cervical HeLa cell line.Methods siRNA targeting HPV18 E6 mRNA was designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA sequence were then transfected into HeLa cells via Lipofectamine()~(TM)2000.Cell viability was determined by MTT assay.Apoptosis was detected by morphological observation and flow cytometry analysis.The expression level of HPV18 E6 mRNA was assayed by RT-PCR.Results The cell growth and viability of(siRNA) transfected group were significantly inhibited(P