1.The emerging role of nuclear factor erythroid 2-related factor 2 signaling pathway in diabetic chronic complications
Chinese Journal of Endocrinology and Metabolism 2017;33(3):185-189
Oxidative stress played an important role in the development of diabetes and its complications. Nuclear factor erythroid 2-related factor 2 (NRF2) pathway is one of the most vital endogenous antioxidant pathways. Accumulated evidences indicated that the relationship between diabetes and NRF2 pathway attracted more and more attention in recent years. Our group has devoted ourselves to the researches concerning the chronic complications of diabetes and NRF2 pathways. This review highlighted our recent progresses in the underlying mechanism of the protective role of NRF2 in diabetic nephropathy, diabetic ulcers and diabetic amyotrophy. Finally, the possibility of NRF2 agonists applied to clinical therapy for diabetic chronic complications was explored.
2.Preconditioning with desflurane, sevoflurane and isoflurane increase the preservation of adenosine triphosphate in anoxia-reoxygenation myocardial cells
Chinese Journal of Clinical Pharmacology and Therapeutics 1999;0(04):-
Aim To study the effects of preconditioning with desflurane, sevoflurane and isoflurane on adenosine triphosphate (ATP) in anoxia-reoxygenation myocardial cells. Methods Rat ventricular myocytes, cultured for 4~5 days, were randomly allocated to five groups: Control group, anoxia-reoxygenation group and groups preconditioned with 1.5 MAC desflurane, sevoflurane or isoflurane following anoxia-reoxygenation. The content of intracellular ATP ,the activities of lactic dehydrogenase(LDH) and creatine kinase(CK), and the cell viability were measured at the end of experiment.Results Preconditioning with 1.5 MAC desfllurane, sevoflurane or isoflurane significantly attenuated the great reduction in ATP and cell viability and the increase of LDH and CK caused by anoxia-reoxygenation. There was a positive correlationship between ATP and cell viability,and a negatiue correlationship between LDH and CK (r was 0.83, -0.87 and -0.82 respectively, P
3.Effect of Mild Hypothermia on NSE and SOD in Serum of Patients with Large-area Cerebral Infarction
Chinese Journal of Rehabilitation Theory and Practice 2006;12(8):661-662
ObjectiveTo investigate the effect of mild hypothermia on neuron specific enolase (NSE) and superoxide dismutase (SOD) in serum of patients with large-area cerebral infarction.Methods160 cases with large-area cerebral infarction were divided into the treatment group and control group with 80 cases in each group. Patients of the control group were treated with routine therapy. Those of the treatment group were added with mild hypothermia therapy (MHT). The scores of NIHSS were assessed and NSE and SOD in serum were examined before treatment and 7, 14 and 30 days after treatment in two groups.ResultsThe NIHSS score of the treatment group was significantly lower than that of the control group ( P<0.05), and NSE level decreased, SOD vitality increased in the treatment group. Other indexes such as respiratory, pulse, serum kalium and etc of two groups were not different ( P>0.05).ConclusionMHT can improve he nerve function of patients with large-area cerebral infarction recovering and improve prognosis.
6.Regulatory Effect of Resveratrol on microRNA in Inflammation Reaction.
Yu CAO ; Min WU ; Long-tao LIU
Chinese Journal of Integrated Traditional and Western Medicine 2016;36(1):125-128
Humans
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Inflammation
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drug therapy
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metabolism
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MicroRNAs
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metabolism
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Stilbenes
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pharmacology
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therapeutic use
7.Biological characteristics of CD90+ tumor stem cells in ovarian cancer cells
Xiaomang JIANG ; Na ZHAO ; Min LONG
Chinese Journal of Tissue Engineering Research 2015;(32):5193-5198
BACKGROUND:There is a close connection between the occurrence and development of tumor stem cels and ovarian cancer. CD90+ is an important tumor stem cel marker.
OBJECTIVE: To explore the biological characteristics of CD90+ tumor stem cels in ovarian cancer cels.
METHODS:The CD133 and CD90 positive rate of SKOV3 and primary ovarian cancer cels were detected by flow cytometry. The CD90+ and CD90- relative expression in stem cels and epithelium was detected by RT-PCR. Transwel invasion assay was employed to observe the cel invasion ability, clone formation test was done to observe cel proliferation and differentiation capacity, suspension bal test was adopted to observe pluripotent stem cels. The tumor formation time and tumor formation rate were observed by limited tumor dilution in immunodeficient mice.
RESULTS AND CONCLUSION:The positive rates of CD133 and CD90 in SKOV3 were significantly lower than those in primary ovarian cancer cels. The expression of CD133 and OCT4 in CD90+cels of SKOVS was significantly higher than that in CD90-cels of SKOVS. The expression of CD44, CD133, acetaldehyde dehydrogenase-1 and OCT4 in CD90+stem cels of primary ovarian cancer cels was significantly higher than that in CD90-stem cels of primary ovarian cancer cels. There were significant differences in the epithelial-mesenchymal related gene expressions between CD90-and CD90+stem cels of SKOV3 and primary ovarian cancer cels. With the increase of inoculated cels, the tumor formation rate of CD90-and CD90+ cels was increased continuously, but the tumor formation time was decreased. The tumor rate of CD90-cels was lower than that of CD90+cels. The number of transmembrane cels, cel clones and suspended cel bals was significantly higher in the CD90+ stem cels than the CD90-stem cels. These findings indicate that in ovarian cancer cels, CD90+stem cels can highly express stem cel-related genes and epithelial-mesenchymal related genes, which have a higher invasion, proliferation and differentiation ability, as wel as tumorigenic and pluripotent ability.
8.Clinical efficacy of laparoscopic splenectomy combined with pericardial devascularization
Jie HUANG ; Kui LONG ; Min SUN
Chinese Journal of Digestive Surgery 2016;15(7):684-688
Objective To investigate the clinical efficacy of laparoscopic splenectomy combined with pericardial devascularization.Methods The retrospective cross-sectional descriptive study was adopted.The clinical data of 64 patients who underwent laparoscopic splenectomy combined with pericardial devascularization at the Second Affiliated Hospital of Kunming Medical University from April 2012 to June 2015 were collected.Observed indexes included (1) treatment outcomes,including surgical procedures,operation time,volume of intraoperative blood loss,time of postoperative enteral recovery,time of postoperative drainage tube removal,duration of postoperative hospital stay,occurrence of complications,(2) follow-up situation.The follow-up using reexaminations of blood routine,liver function,coagulation function,gastroscopy and color Doppler ultrasonography of portal vein was performed regularly at postoperative month 1,2,3,6,12,24 until reemergence of gastrointestinal hemorrhage.The final deadline was death of patients and performance of liver transplantation.Measurement data with normal distribution were presented as-x ± s.Results (1) Treatment outcomes:of 64 patients,62 underwent total laparoscopic splenectomy combined with pericardial devascularization successfully.One patient was transffered to hand-assisted laparoscopic splenectomy combined with pericardial devascularization due to uncontrollable hemorrhage.One patient received laparoscopic cholecystectomy firstly with volume of blood loss of about 1 500 mL and terminated surgery after infusion of suspension red blood cells of 6 U and plasma of 900 mL,and underwent laparoscopic splenectomy combined with pericardial devascularization again next week.No postoperative hemorrhage,pancreatic leakage or death occurred during the perioperative period.The operation time,volume of intraoperative blood loss,time of postoperative enteral recovery,time of postoperative drainage tube removal and duration of postoperative hospital stay were (146 ± 33) minutes,(214 ± 31) mL,(24 ± 4) hours,7 days and (14 ± 6) days,respectively.Nine patients had postoperative pleural effusion and recovered after thoracic drainage and thoracentesis.(2) Follow-up situation:All the 64 patients were followed up for an average time of 19.7 months (range,3.0-23.0 months).Reexamination of gastroscopy showed improvement of varicosed veins of lower esophagus and fundus of stomach.During the postoperative 3 months,4 patients had portal vein thrombosis with level of PLT > 700 × 109/L.For patients with D-Dimer > 5,low molecular weight heparin of 0.4 U was injected subcutaneously until D-Dimer < 2.Three patients were loss to follow-up at postoperative month 6 without upper gastrointestinal hemorrhage,hepatic encephalopathy or liver failure.Conclusion Laparoscopic splenectomy combined with pericardial devascularization is safe and effective for portal hypertension,and rigorous perioperative management offers guarantee for surgical safety.
9.Adverse Reaction of Netilmicin
Min LONG ; Ying WANG ; Rong CHEN ;
China Pharmacy 2001;0(12):-
OBJECTIVE:To explore the relevant factors that led to the adverse drug reaction(ADR)of netilmicin.METHODS:ADR cases resulted from netilmicin reported in12kinds of Chinese periodicals from1998to2003were analyzed statistically.RESULTS:The ADR of netilmicin involved multiple organs and systems,the clinical manifestation were compli-cated and diversified,which manifested mainly on the renal toxicity,toxicity of nervous system and allergic reaction.CON-CLUSION:Clinician and pharmacists should be alert to the ADR of netilmicin and keep using drugs rationally.
10.The role of autonomic nerve system in the pathogenesis of acute mountain sickness
Min LONG ; Jun QIN ; Lan HUANG
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To investigate the role of autonomic nerve system(ANS)in the pathogenesis of acute mountain sickness(AMS).Methods A hypobaric chamber was used to simulate 4500m altitude for 1 day.The function of ANS of 43 healthy young males was measured individually before the experiment,and then at a “height” of 4500m for 5min.GJB1098-91 clinical questionnaires were conducted to evaluate the AMS symptomatic scores.HRV was measured respectively before the test and 2h,10h,20h at 4500m;cold pressure test(CPT)and plasma concentration of NA were determined before the test and at 4500m,AMS scores were evaluated at the end of exposure.25 subjects with AMS scores of 10 or less were identified as mild AMS group(group A),and another 18 subjects with scores of over 10 were gathered as moderately severe AMS group(group B).Results Compared with group A,the systolic pressure(SP)during CPT increased markedly in group B(P