To construct phylogenetic trees based on HPV53 full length sequences, and predict the physical and chemical parameters, secondary structure, B and T cell epitopes of HPV53 proteins(E1, E2, E4, E6, E7, L1, and L2).

Objective To investigate the distribution of pathogenic bacteria,drug sensitivity,and the relationship between drug sensitivity and Escherichia coli(E.coli) induced enzymes in childhood diarrhea in the last 2 years in Chongqing area,so as to provide important evidence for pediatric clinical therapy.Methods Thirty-one E.coli induced enzymes,extended spectrum ?-laetamases(ESBLs),cephalosporinase(AmpC)detected in different phenotype methods,and drug sensitivity was measured in paper strip method,and the specimens were collected from children′s hospital affiliated to chongqing university of medical sciences from Jan.2005 to Dec.2006 were determined.Among the total,there were 18 enteropathogeic E.coli(EPEC) strains,8 enterotoxigenic E.coli(ETEC) strains and 5 enteroinvasive E.coli(EIEC) strains.In addition,drug resistance tests by paper strip included chloramphenicol(CHL),amikacin(AMK),gentamicin(GEN),norfloxacin(NOF),ciproflocacin(CIP),cefazolin(CEZ),cefoperazone(CPZ),ceftriaxone(CRO),ceftazidime(CAZ),cefotacime(CTX),cefepime(FEP),imipenem(IPM).SPSS 12.0 software was used to analyze the data.Results Three point two percent of the 31 E.coli were drug resistant to IPM,and 35.5%,38.7% to NOF,CIP individually,but more than 60% to AMK,GEN,even more than 67.7% towards cephalosporin(except ceftazidime and cefepime);the gross enzyme-produced rate was 87.1%,rate of single ESBLs,AmpC,and induction of both enzymes simultaneously presented 64.5%,6.5%,16.1% respectively;and there was marked difference in drug resistance when bacteria that produced single AmpC versus bacteria that produced single ESBLs or that produced both ESBLs and AmpC(Pa﹤0.05).Conclusions The relationships among enzyme′s quantity,sort and bacterial resistance are different.These data show E.coli infected by bacterial diarrhea children in Chongqing due to a high rate of induced enzymes,and their drug resistance vary according to the state of induced enzymes.

Objective To discuss the cause, managment and prevention of the postoperative complications of orbital implantation by clinical case analysis, and to provide reference for the future clinical work. Methods 175 cases of orbital implantation from Aug. 2011 to Aug. 2013 were retrospectively analyzed in our department. The postoperative complications occurrence, related symptomatic treatment, and the reasons of complications were analyzed. Results Complications occurred in 175 cases of 9 cases(5. 14%),including 4 cases of the con-junctival cyst (2.29%),3 cases of orbital implants exposure(1.71%),1 case of orbital chronic infection(0.57%)and 1 case of subcon-junctival tissue atrophy(0. 57%). All of them had obtained good curative through cyst excision or orbital implanting again after the active treatment. There were no complication occurred again. Conclusion The postoperative complications should be positively analyzed, and early positive precaution could reduce most of the complications.

The regulation how to deal with unskillful and faulty medical treatment is a policy and legal document.It is a implement to the measure how to deal with unskillful and faulty medical treatment. It will make doctor and patients on an equal basis.

Objective To implement a remote monitor system in a core medical laboratory.Methods Firstly,the Hub of StreamLab laboratory automation system was connected to a wireless router to build a wireless network,and several equipments were also integrated into the wireless network using wireless network cards.Secondly,VNC Server was installed into the control PCs of StreamLab LAS,CA7000 Coagulation Analyzer,DPC IMMULITE 2000 SMS and its chemiluminescence photometer,and remote control function of Dimension RxL Max was activated,then some control software such as VNC Viewer,Q/Remote and PC Anywhere were setup in two agency control computers.Finally,PCAnywhere and VNC Viewer were setup in the remote control computers in the Internet and Intranet,respectively.Results A remote control system with three functions was built successfully in a core medical laboratory.Conclusion The remote control system makes the daily operation and remote maintenance of the equipments easy.

Objective To implement a real-time bidirectional communication function on Siemens StreamLab laboratory automation system, Methods Firstly, the system selecting sample was changed from the default settings and then the communication character string sent by the StreamLab was utilized by the laboratory information system to build up a real-time bidirectional control of the samples assay. Results New functions such as real-time signing in and real-time detection information getting of specimens could be performed by the StreamLab automatically.Conclusion The real-time bidirectional communication between laboratory automation system and laboratory information system can optimize the working flow and improve the work efficiency.

Objective:To evaluate the effect of clinical pharmacists in the continued therapy of stroke patients .Methods:Totally 146 cases were selected and randomly divided into the observation group and the control group according to the standard matching meth -od.The observation group was treated with the continued therapy .The control group was given one-month telephone follow-up after the discharge.The differences in the drug compliance (Morisky scale), systolic blood pressure (SBP), diastolic blood pressure (DBP), glycosylated hemoglobin (HbA1C), serum total cholesterol ( TC), low density lipoprotein ( LDL-C), health behaviors ( HPLPⅡscale) and relapse rate were compared between the two groups in the 3rd, 6th and 12th month after the discharge.Results:The differ-ences in the above indices were statistically significant between the two groups ( P <0.05) at the various time points , and those were more significant with time extending (P<0.01).The difference of the relapse rate in the 3rd, 6th and 12th month after the discharge between the two groups were significant .Conclusion: Clinical pharmacists can improve the drug compliance and health behaviors of stroke patients and reduce the relapse rate .It is worthy to be recommended in clinics .

The 'set the duty to offer proof upside down',the conduct and actions put forth effort the exception in the civil case in court as the principle to the deal with medical treatment dispute,it will necessarily provide a good measure and a guarantee to get the doctor-patient relationship for the better.How to treat the 'set the duty to offer proof upside down'is a brand-new topic.Now,the author bing upped some suggestions.

The patient doctor relationship has a favorable turn through preventing ard treating SARS.People have begun to recognize the main factor is ethics problem to the patient doctor relationship.

"medical dispute profiteer" a deterioration of the doctor-patient relationship and sign an inevitable result,it not only undermined the hospital's reputation,disrupting the normal order of medical,but also to build a harmonious society will have an extremely negative impact.The spread of "medical dispute profiteer",attracted academics and the industry's attention.To make medical conduct a comprehensive study of the medical guarantee the normal order and promote health care in a sustained,rapid and healthy development of the broad masses of people to enjoy the harmony of health services is of great significance.