1.The teaching practices of applying simulation techniques combined with PBL in the training before clinical practice
Chinese Journal of Medical Education Research 2003;0(03):-
Objective Toanalyze and evaluate the value ofthe method ofmedical simulation technique combined with PBL pattern in the training before clinical practice. Methods 80 students were divided into two groups:the experimental group and the control group. The PBL method was used in the experimental group,while the control group was taught by traditional method. Results The students in experimental group had a significant higher score than those in control group. Conclu-sion It means that the method of medical simulation combined with PBL teaching is an effective leaning method and should be widely used in the training before clinical practice.
2.Oxidative stress of mitochondrial injury mechanism and targeted antioxidant in sepsis
International Journal of Pediatrics 2014;(5):458-461
Mitochondria is an important part of cell oxidation, but oxidative stress in sepsis as end product of inflammation will cause changes of mitochondria, leading to organ damage. In general, antioxidant defense system fights oxidative stress through the interaction and prevents mitochondrial injury. Oxidative stress plays a important role in organ dysfunction. Studies have found that the targeted therapies for mitochondrial antioxidants may be a new strategy of multiple organ failure caused by sepsis. This review aims to summarize researches on oxidative stress of mitochondrial injury mechanism and targeted antioxidants in sepsis .
3.Rituximab therapy for rheumatoid arthritis
Chinese Journal of Practical Internal Medicine 2001;0(10):-
The mouse/human chimeric anti-CD20 Rituximab(Moab mabthera) can avoid acting on stem cells,pre-B cells and plasma cells;and selectively targets on B cells which have CD20 on their surfaces.Rituximab has been shown to reduce signs and symptoms and to slow the progression of structural damage in adult patients with moderately-to severely-active rheumatoid arthritis in randomised controlled trials and now in the United States has been approved for use in combination with methotrexate(MTX)for the treatment of rheumatoid arthritis by the US Food and Drug Administration.
4.Investigation on Clinical Service Mode of Pharmacists
Guangren LIAO ; Weihua LAI ; Min YANG
China Pharmacy 2005;0(22):-
OBJECTIVE:To explore the suitable clinical service mode for pharmacists in China.METHODS:A questionnaire was designed and a random sample of 170 people were selected from 2 hospitalized patients,doctors,nurses and pharmacists,the results were analyzed using statistical method.RESULTS:There was a general lack of knowledge about clinical pharmaceutical service,higher demands on participation of pharmacists in clinical work were made,and present situation was less than satisfied.CONCLUSION:Clinical service mode of patients first and rational drug use foremost should be established,Chinese pharmacists should improve their knowledge construction and clinical service capability.
6.Effect of Electroacupuncture on Learning and Memory and Heme Oxygenase in Rats with Vascular Dementia
Xinsheng LAI ; Min LI ; Guofeng XU
Journal of Guangzhou University of Traditional Chinese Medicine 2004;0(06):-
[Objective] To observe the effect of electroacupuncture (EA) on the disorder of learning and memory in rats with vascular dementia (VD) and to explore its molecular mechanism. [Methods] Among 60 male SD rats, 10 rats were in sham-operation group and the other 50 rats were used to establish models of global ischemia and reperfusion by four-vessel occlusion (4-VO) method. Then the survival model rats were randomized into EA group (n = 14) , model group (n = 13) and nimotop group (n = 13). EA group was treated with EA at points of Baihui (GV20) and Dazhui (GV14) , nimotop group with nimotop 12 mg/kg, and sham-operation group and model group did not receive any treatment. After a 20-day treatment, the changes of learning and memory behavior in rats were detected by Morris water maze, as well as the protein expression and mRNA expression of heme oxygenase (HO) . [Results] The escape latent period, HO-1 protein expression and HO-1 mRNA-expression were increased obviously in model rats ( P 0.05). [Conclusion] EA can improve the learning and memory behavior in rats with VD and this may be related to the decrease of HO-1 protein expression and HO-1 mRNA-expression in cortex and hippocampal neurons.
7.Relationship between leptin level and glycometabolism in pregnancy with abnormal glucose challenge test
hui-qun, DING ; hui-min, SHU ; lai-min, LUO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
Objective To investigate the relationship between serum leptin(LP) level and glycometabolism in pregnancy with abnormal glucose challenge test(GCT) and the corresponding outcome of pregnancy. Methods Serum LP level,fasting serum insulin(FINS) and fasting blood glucose(FBG) levels were measured by radioimmunoassay in 23 patients with abnormal GCT but normal oral glucose tolerance test(OGTT),20 with impaired glucose tolerance(IGT),20 with gestational diabetes mellitus(GDM)(studied groups above) and 30 normal pregnant women(control group).LP,FINS,FBG, insulin resistance index(HOMA-IR) and ?-cell function index(HOMA-IS) were compared among the groups and the outcomes of pregnancy were observed. Results ①There were significant differences in LP,FINS,FBG,HOMA-IR and HOMA-IS between the studied groups and the control group(P0.05).③There were signi-ficant differences in the gestational weeks of delivery,cesarean section rate and neonatal weight between the studied groups and the control group(P0.05). Conclusion Both insulin secretion and insulin resistance are elevated in pregnancy with abnormal glycometabolism,and are closely correlated to the increased serum level of LP.There existed abnormal glycometabolism in patients with abnormal GCT but normal OGTT,which is correlated to the outcome of pregnancy.
8.Clinical characteristics and related risk factors of Infarction secondary to severe traumatic brain injury
Xiang LAI ; Wenbo ZHANG ; Min YE ; Jinxing HUANG ; Guangyu ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2014;21(1):26-28
Objective To investigate the clinical characteristics and related risk factors of infarction secondary to severe traumatic brain injury.Methods 480 traumatic brain injury patients were chosen.Depending on the occurrence of cerebral infarction,patients were divided into TCI groups and non-TCI group,clinical symptoms and signs of TCI group were observed,and its related risk factors was analyzed.Results In 480 cases patients,there were 30 cases of patients with traumatic brain injury secondary to cerebral infarction,the rate was 6.25%.Clinical manifestations included unilateral limb motor and sensory dysfunction,visual dysfunction,language dysfunction,dizziness,headache.10 cases Prognosis were good,6 cases were mild disability,3 cases were severe disability,1 case was plant survival,10 patients died.Univariate analysis showed that the rates of aged ≥50 years,GCS score < 8 points,hernia,hypotension,subarachnoid hemorrhage,large doses of non-dehydrating agent in the TCI group were higher than those of non-TCI group,the differences were statistically significant (x2 =12.311 3,14.725 4,19.867 8,5.296 9,9.242 6,11.713 6,all P < 0.05).Logistic multivariate analysis showed that age ≥50 years,GCS score < 8 points,hernia,cerebral hypotension were important risk factors.Conclusion Brain injury patients with cerebral infarction secondary to clinical manifestations have some characteristics.Age ≥50 years,GCS score < 8 points,hernia,hypotension are important risk factors.
9.Serum level of galectin 3 and its clinical significance in patients with chronic kidney disease
Dong LAI ; Yongmei XIONG ; Min LONG ; Yiqin ZHANG
Chongqing Medicine 2014;(33):4460-4461
Objective To detect serum galectin 3 levels in patients with chronic kidney disease (CKD) and explore its clinical significance .Methods The galectin 3 levels the serum of in 38 CKD patients and 34 healthy controls were determined by ELISA . All kidney function was measured by automatic biochemical analyzer .The relation between serum galectin 3 levels and the level of kidney function was analyzed by use of t test .Results The serum galectin 3 levels in CKD and healthy controls groups were (1 .22 ± 1 .01)ng/mL and (3 .03 ± 2 .06)ng/mL ,P<0 .05 .There was close negative correlation between serum levels of galectin 3 and Cr ,CysC(P<0 .05) .Conclusion Serum galectin 3 of CKD patients reduces significantly and correlates with kidney function . Detecting on s galectin 3 is helpful for chronic kidney disease diagnosis and therapeutic effect evaluation .
10.Telomerase activation in neoplastic cell immortalization and tumour progression.
Lai Meng Looi ; Min-Hwei Ng ; Phaik-Leng Cheah
The Malaysian journal of pathology 2007;29(1):33-5
The unique ability of tumour cells to proliferate indefinitely is crucial to neoplastic progression as it allows these cells to express the aggressive properties of cancer without the censure of physiological ageing. This is in contrast to normal somatic cells which are subject to a "mitotic clock," a phenomenon that has been linked to telomeric shortening after each round of cell replication, so that eventually the loss of genetic material reaches a critical stage and the cells undergo senescence and cell death. A study was conducted to investigate the role of telomerase, an RNA-containing enzyme that restores the telomere length, in the neoplastic cell immortalization and progression process. Fresh human tissue samples taken from excision specimens received by the Department of Pathology, University of Malaya Medical Centre, were investigated for telomerase activity using a commercial Telomerase PCR-ELISA kit (Boehringer Mannheim). Specimens comprised 33 breast lesions (10 infiltrating breast adenocarcinoma, 13 fibroadenoma and 10 non-neoplastic breast tissue), 27 colonic lesions (17 colonic adenocarcinoma and 10 non-neoplastic colonic mucosa) and 42 cervical lesions (20 cervical carcinoma and 22 non-neoplastic cervical tissues). Telomerase activity was found in 6 (60%) of 10 breast carcinomas, 6 (46%) of 13 fibroadenomas, none of the 10 nonneoplastic breast samples, 3 (17.6%) of 17 colon carcinomas and none of the 10 non-neoplastic colonic mucosal samples, 12 (60%) of 20 cervical carcinoma and 3 (13.6%) of 22 non-neoplastic cervical samples. 5/10 (50%) Stage I, 4/7 (57%) Stage II, 2/2 (100%) Stage III and 1/1 (100%) Stage IV cervical carcinomas showed telomerase activity. These findings support a contributory role for telomerase in tumourigenesis with activation occurring from neoplastic transformation and increasing with tumour progression.
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