1.Cognitive deficits of the schizophrenics.
Jae Joong SHIN ; Min Kyou LEE ; Sang Hag PARK
Journal of Korean Neuropsychiatric Association 1991;30(5):815-823
No abstract available.
2.Cognitive deficits of the schizophrenics.
Jae Joong SHIN ; Min Kyou LEE ; Sang Hag PARK
Journal of Korean Neuropsychiatric Association 1991;30(5):815-823
No abstract available.
3.The influence of H1, H2-histamine antagonists and disulfiram to ethanol and acetaldehyde patch test results.
Shin CHUNG ; Hack Ryul KIM ; Min Kyou LEE
Journal of Korean Neuropsychiatric Association 1991;30(1):54-65
No abstract available.
Acetaldehyde*
;
Disulfiram*
;
Ethanol*
;
Patch Tests*
4.The effects of token economy program for a psychiatric patient with regressive behavior.
Myung Won JUNG ; Min Kyou LEE ; Kyung Chae JOO
Journal of Korean Neuropsychiatric Association 1993;32(2):259-265
No abstract available.
Humans
;
Token Economy*
5.The effects of cognitive expectancies of alcohol on the drinking.
In Bok HWANG ; Min Kyou LEE ; Kyung Chae JOO
Journal of Korean Neuropsychiatric Association 1993;32(6):962-970
No abstract available.
Drinking*
6.Immunosuppressive Effects of Tautomycetin on T Cells.
Heug Kyu LEE ; Kyung Min CHO ; Hyoung Sik CHUN ; Hyeog Jin SON ; Sang Kyou LEE
Korean Journal of Immunology 1998;20(2):85-90
T cell activation is a critical event for initiation and regulation of immune responses and inhibitors of such signaling pathways are clinically useful for the treatment of patients received allogratt and autoimmune disease. In the course of screening soil microorganisms from the forest of Cheju island in Korea for new immunosuppressive agent, one of Streptomyces species (CK-95441) was found to produce a new immunosuppressant, tautomycetin which also had antifungal activity. Tautomycetin showed the inhibition of T cell proliferation in murine mixed lymphocyte reaction (MLR) and T cell activation induced by concanavalin A. Tautomycetin also blocked the induction of IL-2 gene expression which was examined in Jurkat TAg cell line in which multiple NFAT-binding sites and minimal IL-2 promoter drive the production of B-galactosidase. Also, the level of inhibition in activation-induced IL-2 receptor expression by tautomycetin was greater than those by cyclosporin A measured by flow cytometry. But, Fas ligand-induced apoptosis in Jurkat cells was unaffected by tautomycetin which was measured by DNA fragmentation assay. These results suggested that tautomycetin will be able to be used as a potent immunosuppressive drug following organ transplantation.
7.T Cell-specific Immunosuppression Using Tautomycetin or PTD-conjugated Protein Drugs.
Wook Jin CHAE ; Je Min CHOI ; Jung Jin YANG ; Sang Kyou LEE
Yonsei Medical Journal 2004;45(6):978-990
No abstract available.
Animals
;
Antibiotics, Antifungal/*therapeutic use
;
Humans
;
Immunosuppression/*methods
;
Protein Structure, Tertiary
;
T-Lymphocytes/*immunology
;
*Transduction, Genetic
8.Okadaic Acid, RK682 and Calyculin Modulate TcR - Mediated Signaling Events.
Sang Kyou LEE ; Jung Hee LIM ; Kyung Min CHO ; Hyun Jung KIM ; Sang Won KIM ; Young Sup SONG
Korean Journal of Immunology 1997;19(3):327-336
The T cell antigen receptor (TcR) in combination with costimulatory signals triggered by accessory molecules present on the surface of the antigen-presenting cells (APC) regulates the activation and growth of T lymphocytes. Calyculin A and Okadaic acid is known to be an inhibitor of serine/threonine phosphatase and RK-682 specifically blocks functions of tyrosine phosphatase. To investigate roles of these inhibitors in TcR-mediated signaling cascade, chimeric molecule CD8-5 which contains the extracellular and transmembrane domains of the human CD8a molecule and the cytoplasmic tail of TcR 5 chain were stably expressed in Jurkat cell line. CD8-5 chimeric protein induced tyrosine phosphorylation of various cytoplasmic substrates and IL-2 gene expression in a NFAT dependent manner by stimulation with anti-CD8 mAb OKT8 as seen in TcR stimulation. When CD8-5 transfectants were preincubated with Okadaic acid, Calyculin or RK682, they differentially affected tyrosine phosphorylation of signaling mediators including CD8-5 molecule. When Jurkat Tag cell line was used where SV40 T antigen is stably expressed and the expression of p-galactosidase is driven by the multiple NFAT binding sites plus minimal IL-2 promoter, these phosphatase inhibitors -RK682, Calyculin A, Okadaic acid- effectively inhibited IL-2 gene expression at the concentration of 1.2832 x 10 ' M, 3.9924 x 10 M, 7.2707 x 10 M respectively. These results suggested that Okadaic acid, Calyculin or RK682 modulate TcR-proximal as well as TcR-distal signaling events during T cell activation.
Antigen-Presenting Cells
;
Antigens, Viral, Tumor
;
Binding Sites
;
Cell Line
;
Cytoplasm
;
Gene Expression
;
Humans
;
Interleukin-2
;
Jurkat Cells
;
Okadaic Acid*
;
Phosphorylation
;
Receptors, Antigen, T-Cell
;
T-Lymphocytes
;
Tyrosine
9.A Novel Cell Line for Screening of Immunosuppressor Specific to T Lymphocytes.
Sang Kyou LEE ; Jung Hee LIM ; Kyung Min CHO ; Seung Hyo LEE ; Yong Sup SONG ; Hyoung Sik CHUN ; Hyeog Jin SON
Korean Journal of Immunology 1997;19(3):375-382
The systematic study of products from bacteria and fungi has led to the development of two immunosuppressive drugs, cyclosporin A and FK 506 (tacrolimus) which are useful to suppress adaptive immune responses to the grafted tissue. However, they affect all immune responses indiscriminately and are both toxic to kidneys and other organs. To facilitate the development of immunosuppressor to block the T cell receptor (TcR)-mediated signaling cascade specifically, a novel Jurkat T cell transfectants, JK NFAT-SEAP were generated in which the expression of the secreted alkaline phosphatase (SEAP) is driven by the multiple NFAT binding sites plus minimal IL-2 promoter. Upon stimulation with ionomycin or anti-TcR mAb OKT3 in the presence of PMA, these transfectants secreted high level of SEAP into the medium, which was conveniently analyzed by SEAP analysis. The secretion of SEAP was effectively inhibited by cyclosporin A or FK 506 at the concentration of [10 ' ug/ml], [10 ug/ml] respectively. JK NFAT-SEAP transfectants will provide two major advantages for the development of a novel immunosuppressor. First, analysis of SEAP secreted into the culture medium by SEAP analysis enables us to test a large number of samples within a short period of time. Second, Usage of IL-2 promoter for the expression of SEAP makes us identify bioproducts to target specifically on TcR-mediated signaling pathway.
Alkaline Phosphatase
;
Bacteria
;
Binding Sites
;
Cell Line*
;
Cyclosporine
;
Fungi
;
Interleukin-2
;
Ionomycin
;
Kidney
;
Mass Screening*
;
Muromonab-CD3
;
Receptors, Antigen, T-Cell
;
T-Lymphocytes*
;
Tacrolimus
;
Transplants
10.Peripapillary Choroidal Thickness Change of Polypoidal Choroidal Vasculopathy after Anti-vascular Endothelial Growth Factor.
Kyou Ho LEE ; Seo Hee KIM ; Ji Min LEE ; Eui Chun KANG ; Hyoung Jun KOH
Korean Journal of Ophthalmology 2017;31(5):431-438
PURPOSE: To investigate the peripapillary choroidal thickness (PCT) of polypoidal choroidal vasculopathy (PCV) and exudative age-related macular degeneration (AMD) and to evaluate their responses to anti-vascular endothelial growth factor (VEGF). METHODS: Thirty eyes with PCV and 25 eyes with exudative AMD who were treatment naïve were included in this study. PCT and subfoveal choroidal thickness were evaluated both before and after intravitreal anti-VEGF. RESULTS: The initial mean PCT of PCV (153.78 ± 56.23 µm) was thicker than that of exudative AMD (88.77 ± 23.11 µm, p < 0.001). Temporal, superior, nasal, and inferior PCTs of PCV were all thicker than those observedin exudative AMD (all p < 0.05). After anti-VEGF, the mean PCT of PCV was significantly reduced (134.17 ± 41.66 µm, p < 0.001), but the same was not true not in exudative AMD (86.87 ± 22.54 µm, p = 0.392). PCTshowed a similar tendency in all quadrants. CONCLUSIONS: PCV exhibits a thick choroid in the peripapillary region. PCT decreases after anti-VEGF in PCV but not in exudative AMD. In exudative AMD, subfoveal choroidal thickness decreased, but that in the peripapillary region did not.
Choroid*
;
Endothelial Growth Factors*
;
Macular Degeneration