1.Molecular characterization of genomic DNA and antifungal susceptibility of candida albicans.
Journal of the Korean Society for Microbiology 1992;27(2):173-180
No abstract available.
Candida albicans*
;
Candida*
;
DNA*
2.Detection of varicella-zoster virus DNA in clinical specimens by polymerase chain reaction(PCR).
Min Ho SUH ; Won Ki BAEK ; Kyu Suk LEE
Journal of the Korean Society for Microbiology 1991;26(5):479-486
No abstract available.
DNA*
;
Herpesvirus 3, Human*
3.Spontaneous Testicular Hemorrhagic Necrosis Masquerading as a Testis Tumor .
Seung Hyun BAEK ; Jun Baek PARK ; Yun Hyung JANG ; Yeon Won PARK ; Jin Hyung LEE ; Seung Ki MIN
Korean Journal of Urology 2004;45(9):962-965
Spontaneous testicular hemorrhagic necrosis is a rare disease usually associated with testicular torsion. Partially involved and suspicious testis tumor cases are also defined from orchiectomy specimens. Herein, a spontaneous hemorrhagic necrosis, without any testicular torsion, but with involvement of the whole testicle and epididymis, is reported. A 21 year old patient, who presented with a painless left testicular enlargement of several days duration was believed, based on physical examination, ultrasonography and elevation of serum LDH, to have a testicular tumor. Diagnosis was made only after radical orchiectomy and histopathological examination.
Diagnosis
;
Epididymis
;
Hemorrhage
;
Humans
;
Male
;
Necrosis*
;
Orchiectomy
;
Physical Examination
;
Rare Diseases
;
Spermatic Cord Torsion
;
Testis*
;
Ultrasonography
;
Young Adult
4.Detection of human cytomegalovirus DNA polymerase gene by polymerase chain reaction.
Hyun Chul KIM ; Sung Bae PARK ; Won Hyun CHO ; Won Ki BAEK ; Min Ho SUH
Journal of the Korean Society for Microbiology 1992;27(2):181-188
No abstract available.
Cytomegalovirus*
;
DNA*
;
Humans*
;
Polymerase Chain Reaction*
5.Transecleral Suture Fixation for a Posterior Chamber Intraocular Lens Implantation.
Yong Baek KIM ; Woog Ki MIN ; Byung Heon AHN
Journal of the Korean Ophthalmological Society 1990;31(4):445-453
Posterior chamber intraocular lenses were implanted into 20 eyes without the posterior capsular capsular support by securing both haptics of the lens to the sclera with 10-0 prolene suture. The follow-up period ranged from 6 to 24 months(average 9.4 months). At the last follow-up visit, 17 eyes(85%) had a vision of 0.7 or better. The position of the implanted lens was proved satisfactory when compard with eyes with posterior chamber intraocular lens without scleral fixation suture by postoperative A-scan measurement of the anterior chamber depth, the results of refraction and slit-lamp examination. There were no eyes with remarkable decentration or tilt of the implanted lens. In two eyes, intraoperative bleeding occurred from the inferior chamber angle and ceased spontaneously. Postoperatively there were no remarkable inflammatory responses or complications. From the results, we suggest that the position of scleral puncture with a hypodermic needle is 1.2 - 1.4mm from the posterior surgical limbus in the upper quadrant and the advancement of the needle is directing parallel to the posterior surface of the iris.
Anterior Chamber
;
Follow-Up Studies
;
Hemorrhage
;
Iris
;
Lens Implantation, Intraocular*
;
Lenses, Intraocular*
;
Needles
;
Polypropylenes
;
Punctures
;
Sclera
;
Sutures*
6.Effect of Retrovirus and p53 Tumor Suppressor Gene in Gene Therapy of Cancer Cells.
Seong Il SUH ; Min Ho SUH ; Won Ki BAEK ; Jae We CHO
Journal of the Korean Society for Microbiology 1998;33(2):227-235
Cancer is considered to occur through abnormal growth and differentiation processes, in which oncogenes and tumor suppressor genes are deeply related. Cellular responses to DNA-damaging agents are believed to be critical determinants of human tumorigenesis. Cell cycle arrests and DNA repair following DNA damage require the coordination of multiple gene products that, as a whole, serve to maintain the integrity of the genome. Within the cell cycle, both G1-S and G2-M phase transitions are under constant surveillance by checkpoint genes for the protection of cells from either exogenous or endogenous DNA-damaging agents. p53 tumor suppressor gene mediates cell cycle perturbations in response to DNA damage, and play a role in cell death, genetic stability, and cancer susceptibility. Recently, gene therapy with p53 tumor suppressor gene is expected as a new effective therapeutic strategy in many kinds of cancer. By using retroviral vector system, we transduced p53 tumor suppressor gene into human osteosarcoma cells, and analysed its growth suppression and apoptosis inducing effects. Combined effects of p53 gene therapy with chemotherapeutic agent or radiation were also analysed. Titer of ecotrophic p53 retrovirus was 5.0x10/ml, and that of amphotrophic p53 retrovirus was 2.0x10/ml when NIH3T3 cells were used as target cells. Human osteosarcoma cells infected with amphotrophic p53 retroviruses showed increased p21waf1 gene expression, which acts as a major cyclin-dependent kinase inhibitor in DNA damage responses. In normal DMEM media, human skin fibroblasts infected with amphotrophic p53wt retroviruses showed very slow growing (1.7 fold increase in doubling time) and very low saturation density (50% decrease in cell density). In media containing chemotherapeutic agent, human osteosarcoma cells infected with p53wt retroviruses died rapidly; 75% of them died within 4 days and all of them died within 10 days of incubation with chemotherapeutic agent. Their DNAs were extracted and electrophoresed in agarose gel, and we identified DNA ladders characteristic of apoptotic cell death. When human osteosarcoma cells infected with p53 retroviruses were irradiated with ultraviolet light, more than 95% of cancer cells died within 1 day; whereas mock infected cells showed only less than 5% of cell death. These findings suggest that retroviral vector mediated p53 tumor suppressor gene transfer into cancer cells can suppress tumor cell growth and decrease tumor cell density effectively. These findings also suggest that effective induction of tumor cell apoptosis can be obtained when p53 gene therapy is used in combination with chemotherapy or radiotherapy.
Apoptosis
;
Carcinogenesis
;
Cell Count
;
Cell Cycle
;
Cell Cycle Checkpoints
;
Cell Death
;
DNA
;
DNA Damage
;
DNA Repair
;
Drug Therapy
;
Fibroblasts
;
Gene Expression
;
Genes, p53
;
Genes, Tumor Suppressor*
;
Genetic Therapy*
;
Genome
;
Humans
;
Oncogenes
;
Osteosarcoma
;
Phase Transition
;
Phosphotransferases
;
Radiotherapy
;
Retroviridae*
;
Sepharose
;
Skin
;
Ultraviolet Rays
;
Zidovudine
7.Antimicrobial drug susceptibility of enterobacter and citrobacter islated from clinical specimens.
Sung Duck PAIK ; Won Ki BAEK ; Seong Il SUH ; Jong Wook PARK ; Min Ho SUH
Journal of the Korean Society for Microbiology 1993;28(6):443-451
No abstract available.
Citrobacter*
;
Enterobacter*
8.Detection of hepatitis B virus DNA in serum by dot blot hybridization.
Min Ho SUH ; Seong Il SUH ; Won Ki BAEK ; Sang Sook LEE ; Jae Ryong KIM
Journal of the Korean Society for Microbiology 1992;27(1):87-92
No abstract available.
Hepatitis B virus*
;
Hepatitis B*
;
Hepatitis*
9.Detection of hepatitis B virus DNA in serum by dot blot hybridization.
Min Ho SUH ; Seong Il SUH ; Won Ki BAEK ; Sang Sook LEE ; Jae Ryong KIM
Journal of the Korean Society for Microbiology 1992;27(1):87-92
No abstract available.
Hepatitis B virus*
;
Hepatitis B*
;
Hepatitis*
10.Antimicrobial resistance of escherichia coli isolated from clinical specimens.
Sung Duck PAIK ; Won Ki BAEK ; Seong Il SUH ; Jong Wook PARK ; Min Ho SUH
Journal of the Korean Society for Microbiology 1993;28(4):261-267
No abstract available.
Escherichia coli*
;
Escherichia*