1.Ginseng may modify the progression of degenerative cerebellar ataxia: A report of two case
Min Jung Oh ; Min-Wook Kim ; Manho Kim
Neurology Asia 2015;20(3):313-318
Cerebellar degeneration is a group of diseases that manifests as progressive ataxia, that finally led to
death without specific treatment. We report here two patients with cerebellar degeneration, who had
shown an improvement and less progressive course, which is associated with panax ginseng intake.
Patient 1 was a 60-year-old woman with multisystem atrophy (MSA) type C with 5 year history of
ginseng ingestion. Patient 2 was a 54-year-old woman with spinocerebellar ataxia (SCA) type 6,
who had a history of ginseng intake for 30 months. Both the patients showed atrophic change in the
cerebellum by brain magnetic resonance imaging. Cerebellar functions had been semi-quantified by
International Cooperative Ataxia Rating Scale (ICARS) and monitored before and after the ginseng
ingestion every 6 to 12 months. In Patient 1 with MSA type C, ICARS had improved from 21 to 17.5
± 1.8 in the following 5 years. In Patient 2 with SCA, ICARS also showed an improvement from
22 to 6.0 ± 1.0 over 30 months. However, when she stopped taking ginseng, it progressed up to 13
points in two years. These observations provide a potential disease-modifying effect of ginseng on
patients with cerebellar degeneration.
Cerebellar Ataxia
;
Cerebellar Diseases
2.Vitamin K-Deficient Hemorrhagic Disease in Infants with Acute Subdural Hematoma.
Kyu Yong CHO ; Sin JUNG ; Min Suk OH
Journal of Korean Neurosurgical Society 1991;20(10-11):924-929
We had experienced 2 cases of acute subdural hematoma due to vitaimin K(vit. K) deficiency. They were 44-and 42-day-old. After the adminstration of vit.K1, prolonged prothrombin time(PT) and activated partial thromboplastin time(APTT) were corrected, so the surgical treatment ws performed. We supposed the predisposing factors were breast feeding and unknown hepatic pathology. No complication remained to one infant but the other multiple cerebral infarctions. The etiology, pathogenesis, diagnosis, prevention are discussed. We insist on neonatal administration of vit. K1 for the prevention of bleeding tendency. If intracranial hemorrhage develops, we must keep in mind that the early neurosurgical intervention may be needed.
Breast Feeding
;
Causality
;
Cerebral Infarction
;
Diagnosis
;
Hematoma, Subdural, Acute*
;
Hemorrhage
;
Humans
;
Infant*
;
Intracranial Hemorrhages
;
Partial Thromboplastin Time
;
Pathology
;
Prothrombin
;
Prothrombin Time
;
Thromboplastin
;
Vitamin K Deficiency
;
Vitamins*
3.Comparison of Polymerase Chain Reaction and DNA Hybridization for Detection of the Cholera Toxin Operon of Vibrio cholerae.
Yung Bu KIM ; Yang Hyo OH ; Min Jung KIM
Journal of the Korean Society for Microbiology 1998;33(6):547-555
Cholera enterotoxin (CT) is a major virulence determinant of Vibrio cholerae 01. CI' is known to be the major virulence factor of Vibrio cholerae 01 and in accordance with the recent report showing which V. cholerae non-01 has ctx gene, we performed the molecular genetic study for the detection of ctx gene related to the production of CT at the subject Vibrio spp. except for V. cholerae non-01 and V. cholerae non-01 stock cultured in the laboratory of microbiology, College of Medicine, Pusan National University and the Vibrio spp. isolated from the marine products of Pusan General Fish Market and the sea water, and then its results are as follows: 1. PCR for the detection of ctx gene at the subject of V. cholerae 01:61H-151 having the ctx gene of which the denaturation is 1 rninute at 95'C, annealing to 1min, 30 sec at 60'C, the extension to be 1min. 30 sec at 72'C and 30 or 40 cycles. ctx gene was detected from 4 strains of V. cholera non-01 derived from the environment isolates. 2. Adjusting the quantity of chromosomal DNA used as template DNA to be from 0.1 pg to 1 ng, in order to know the PCR conditions for the effective search of ctx gene, and the detection limit of the system was 10 pg of chromosomal DNA. 3. The broth culture was used for template DNA, ctx gene of 302 bp was detected from 4 V. cholerae non-01, as in the case of chromosomal DNA, and the cell number was possible to be detected to 3 * 10.4. We attempted the confirmation of ctx gene through Southern blot hybridization, labeling with P and then it was confirmed only from 4 V. cholerae non-01 as like PCR results. 5. As the result of the sensitivity of PCR and Southern blot hybridization, it was shown to be possible which 10 pg was detected in case of chromosomal DNA and in case of cultured broth, the cell number was detected until 10 at PCR and Southern blot hybridization, and thus it was examed which its sensitivity was same.
Blotting, Southern
;
Busan
;
Cell Count
;
Cholera Toxin*
;
Cholera*
;
DNA*
;
Enterotoxins
;
Limit of Detection
;
Molecular Biology
;
Operon*
;
Polymerase Chain Reaction*
;
Seawater
;
Vibrio cholerae*
;
Vibrio*
;
Virulence
4.A study of surgical management for peptic ulcer.
Min Oh LEE ; Tae Hyung CHO ; Yang Soo JUNG
Journal of the Korean Surgical Society 1991;40(2):158-167
No abstract available.
Peptic Ulcer*
5.Four cases of malignant mixed mullerian tumors of uterus.
Jung Hee AHN ; Jong Chan PARK ; Min Jung OH ; Hae Jung KIM ; Kyu Wan LEE
Korean Journal of Obstetrics and Gynecology 1993;36(8):3343-3350
No abstract available.
Uterus*
6.Development of a Rapid Detection Method for Yersinia pestis by Polymerase Chain Reaction.
Ho Jung OH ; Hong Ki MIN ; Yeo Won SOHN ; Jeong Hoon CHUN ; Han Oh PARK
Journal of the Korean Society for Microbiology 1999;34(4):373-383
A polymerase chain reaction (PCR) method for detection of the pathogenic Yersinia pestis from other Yersinia spp. was developed. Five Y. pestis strains, ninety-two other Yersinia species and twenty-four Enterobacteriaceae strains were collected in Korea and from other countries. Oligonucleotide primers were designed from pathogenic gene of antiphagocytic protein capsule gene (fra 1) and plasminogen activator gene (pla). The 428 bp DNA fragment was amplified from five Y. pestis which contained the fra I gene. No product was amplified from other Yersinia species and other strains of the Enterobacteriaceae. The 439 bp DNA fragment was amplified from three K pestis which contained the pla gene. No product was amplified from two Y. pestis, other Yersinia species and other strains of the Enterobacteriaceae. These showed that the designed primers were specific for detection of Y. pestis among other Yersinia species and Enterobacteriaceae strains. Amplification was successful whether the template was derived from purified DNA or from aliquots of boiled bacterial suspension. The detection limits were 100 pg of DNA and 100 colony forming units (CFU) for fra I and 100 pg DNA and 10 CFU for pla, respectively. Our results prove that the PCR method using specific primers for Y. pestis is a rapid and convenient procedure for routine clinical detection and identification of Y. pestis.
DNA
;
DNA Primers
;
Enterobacteriaceae
;
Korea
;
Limit of Detection
;
Plasminogen Activators
;
Polymerase Chain Reaction*
;
Stem Cells
;
Yersinia pestis*
;
Yersinia*
7.Roles of FGF-4 on the Differentiation of Trophoblast Stem (TS) Cells.
Min Kyu CHOI ; Jung Taek OH ; Jay Min OH
Korean Journal of Anatomy 2004;37(2):141-148
Fibroblast growth factor-4 (FGF-4) has various functions, affecting many signaling pathways, and leading to cellular proliferation and differentiation and to the regulation of cell migration, invasion, and angiogenesis. However, there are few reports of the relationship between TS cells and FGF-4 even if FGF-4 is located in inner cell mass of embryo and Fibroblast growth factor receptor (FGFR) is located in TS cells. Therefore the physiologic effects of FGF-4 on TS cells were investigated for identifying the effects of FGF-4 on TS ell differentiation. FGF-4 was involved in early stage development of the trophoblast via upregulation of eomesodermin mRNA expression. In addition, FGF-4 suppressed the differentiation of TS cells through activation of extracellular-signal regulated kinase (Erk) and suppression of focal adhesion kinase (FAK) activation, which in TS cells is an important indicator of early trophoblast cell differentiation, migration and invasion. FGF-4 was involved in angiogenesis in the trophoblast through the activation of p38 and the induction of Dlx-3 mRNA expression in TS cells. In addition, TS cells cultured with FGF-4 for 4 days in a thrombinfibrinogen gel culture system, a specific culture system for endothelial cells, showed a healthy appearance, while TS cells cultured without FGF-4 were severely damaged. Taken together, these data suggest that FGF-4 is closely involved in differentiation of TS cells for development of placenta.
Cell Differentiation
;
Cell Movement
;
Cell Proliferation
;
Embryonic Structures
;
Endothelial Cells
;
Fibroblasts
;
Focal Adhesion Protein-Tyrosine Kinases
;
Phosphotransferases
;
Placenta
;
Receptors, Fibroblast Growth Factor
;
RNA, Messenger
;
Trophoblasts*
;
Up-Regulation
8.Maternal Cell Contamination in Chorionic Villus Sampling : An Analysis of Frequency in 1,059 Consecutive Cases.
Bo Hoon OH ; Jung Min LEE ; Gyung Hwa LEE ; Ae Young JUNG ; Moo Sik GWON
Korean Journal of Obstetrics and Gynecology 2000;43(8):1384-1388
No abstract available.
Chorion*
;
Chorionic Villi Sampling*
;
Chorionic Villi*
;
Female
;
Pregnancy
9.Refined correction method of unilateral cleft lip nasal deformity.
Yeon Chul JUNG ; jin Hwan KIM ; Rong Min BAEK ; Kab Sung OH ; Se Min BAEK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1993;20(5):1006-1013
No abstract available.
Cleft Lip*
;
Congenital Abnormalities*
10.10-YEAR EXPERIENCE ON REDUCTION MALARPLASTY.
Jung Wook HAHM ; Rong Min BAEK ; Kap Sung OH ; Se Min BAEK
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1997;24(6):1478-1487
No abstract available.