No abstract available.
Avidin*

Kaposi's sarcoma was first descrided by Kaposi in 1872 as an idiopathic multiple hemorrhagic sarcoma. Its clinical features revealed to be erythematous red or purple macule started out, and developing into palpable dome-shaped nodules. Etiology is not defined to detail at present. Kaposi's sarcoma is classified to 4 categories; Classical, African, Epidemic and Transplant type. Epidemic or AIDS categories is found approximately 20 % of all AIDS and has strong predilection for head and neck region. The first case of Kaposi's sarcoma observed in patients with kidney transplants was reported in 1969. Kaposi's sarcoma now accounts for 5% of all tumors associated with transplanted patients and alteration of the immunosuppression may have played a key role in these recipients. The most common site of Kaposi's sarcoma in transplanted patients are extremities but rare in head and neck area. We report a case of Kaposi's sarcoma on the hard palate in the kidney transplantation patient.
Extremities ; Head ; Humans ; Immunosuppression ; Kidney ; Kidney Transplantation ; Mouth* ; Neck ; Palate, Hard ; Sarcoma ; Sarcoma, Kaposi*

Cancer tissues are characterized by increased glucose uptake. 18F-fluorodeoxyglucose(FDG), a glucose analogue is used for the diagnosis of cancer in PET studies. This study was aimed to compare the glucose uptake and glucose transporter l(GLUT1) expression in various human colon cancer cells. We measured FDG uptake by cell retention study and expression of GLUTI using Western blotting. Human colon cancer cells, SNU-C2A, SNU-C4 and SNU-C5, were used. The cells were incubated with 1micro Ci/ml of FDG in HEPES-buffered saline for one hour. The FDG uptake of SNU-C2A,SNU-C4 and SNU-C5 were 16.8+/-1.36, 12.3+/-5.55 and 61.0+/-2.17cpm/microgram of protein, respectively. Dose-response and time-course studies represent that FDG uptake of cancer cells were dose dependent and time dependent. The rate of FDG uptake of SNU-C2A, SNU-C4 and SNU-C5 were 0.29+/-0.03, 0.21+/-0.09 and 1.07+/-0.07cpm/min/microgram of protein, respectively. Western blot analysis showed that the GLUT1 expression of SNU-C5 was significantly higher than those of SNU-C2A and SNU-C4. These results represent that FDG uptake into human colon cancer cells are different from each other. In addition, FDG uptake and expression of CLUT1 are closely related in human colon cancer cells.
Blotting, Western ; Colon* ; Colonic Neoplasms* ; Diagnosis ; Glucose ; Glucose Transport Proteins, Facilitative ; Humans*

In order to prevent the formation of postoperative adhesions and delay the time of adjustment, the authors tried 5-fluorouracil and physical barriers of polytetrafluoroethylene, Interceed, Viscoat in rabbits. Fluorouracil delayed the adjustment for up to one week after surgery in three out of four eyes. Combined use of Interceed and 5-fluorouracil of addition of Viscoat could delay the adjustment for up to two weeks after surgery I one out of four eyes. Combined use of pophtetrafluoroethylene and 5-fluorouracil of addition of Viscoat could delay the adjustment for up to four weeks after surgery in four out of five eyes. In conclusion, combined use of polytetrafluoroethylene, 5-fluorouracil and Viscoat could be more helpful for delayine adjustment in rabbits.
Fluorouracil* ; Polytetrafluoroethylene ; Rabbits ; Strabismus*

Lebers hereditary optic neuropathy(LHON)is caused by a single nucleotide change in the mitochondrial deoxynucleic acid(mtDNA)and accounts for 30% of bilateral optic atrophy of unknown etiology. In order to define the clinical features of LHON associated with the 11778 mtDNA mutation, clinical and historical data were collected from 60 visually symptomatic patients with the molecularly confirmed mtDNA 11778 mutation. Forty-nine(82%)of the 60 patients were male. Onset of visual loss was between 6 and 63 years of age. Worst visual acuity ranged from light perception to 0.8. In most patients, visual acuities deteriorated to worse than 0.1 and remained at that level. Final visual acuities ranged from light perception to 1.2.Five(8.3%)out of 60 patients showed improvement of visual acuity up to 0.5 or better in at least one eye. Ishihara test revealed severe color vision impairment in most patients. Optic atrophy was the most common ophthalmoscopic finding.However, increased C/D ratio or normal disc was also observed. Central scotoma was the most frequent visual field defect, but normal visual field was also found. LHON should be considered in the differential diagnosis of optic neuropathy in a young man with visual and color deterioration as well as with a central scotoma. However, various phenotypes also exist in an atypical LHON case.
Color Vision ; Diagnosis, Differential ; DNA, Mitochondrial* ; Humans ; Male ; Optic Atrophy ; Optic Nerve Diseases* ; Phenotype ; Scotoma ; Visual Acuity ; Visual Fields

No abstract available.
Colorectal Neoplasms* ; Follow-Up Studies*

No abstract available.
Colorectal Neoplasms* ; Follow-Up Studies*

PURPOSE: For the purpose of using Re-188 as a therapeutic radionuclide, we performed the quality control of the W-188/Re-188 generation system. MATERIALS AND METHODS: Several quality control tests of the Re-188 eluate from generator were carried out of about 300 days. After elution of Re-188 with normal saline (20 ml), chromatogram and gamma-ray spectrum of Re-188 eluate were obtained. The presence of aluminum which was derived from the elumina bed of the generator was detected by using aluminum ion indicator kit. Re-188 eluate was allowed to decay for several days, and then W-188 breakthrough in the Re-188 eluate was measured by detecting gamma-ray at 227 keV and 290 keV. The pH and the pyrogenicity of the eluate were checked. The Re-188 eluate from the generator was 67.4+/-7.0% of W-188 during 270 days, and it was hightest at third day after previous elution. Radiochemical purity of Re-188 eluate obtained from chromatogram was higher than 99%. Gamma-ray spectrum of Re-188 eluate showed a peak at 155 keV. Aluminum ion and W-188 contamination were not detected. The pH of Re-188 eluate was 3 and the concentration yield was 85%. CONCLUSION: Our experiments and results on quality control tests of Re-188 eluate from W-188/Re-188 generator may be useful for setting W-188/Re-188 generator in hospitals.
Aluminum ; Hydrogen-Ion Concentration ; Quality Control*

PURPOSE: For the purpose of using Re-188 as a therapeutic radionuclide, we performed the quality control of the W-188/Re-188 generation system. MATERIALS AND METHODS: Several quality control tests of the Re-188 eluate from generator were carried out of about 300 days. After elution of Re-188 with normal saline (20 ml), chromatogram and gamma-ray spectrum of Re-188 eluate were obtained. The presence of aluminum which was derived from the elumina bed of the generator was detected by using aluminum ion indicator kit. Re-188 eluate was allowed to decay for several days, and then W-188 breakthrough in the Re-188 eluate was measured by detecting gamma-ray at 227 keV and 290 keV. The pH and the pyrogenicity of the eluate were checked. The Re-188 eluate from the generator was 67.4+/-7.0% of W-188 during 270 days, and it was hightest at third day after previous elution. Radiochemical purity of Re-188 eluate obtained from chromatogram was higher than 99%. Gamma-ray spectrum of Re-188 eluate showed a peak at 155 keV. Aluminum ion and W-188 contamination were not detected. The pH of Re-188 eluate was 3 and the concentration yield was 85%. CONCLUSION: Our experiments and results on quality control tests of Re-188 eluate from W-188/Re-188 generator may be useful for setting W-188/Re-188 generator in hospitals.
Aluminum ; Hydrogen-Ion Concentration ; Quality Control*

No abstract available.
Child* ; Evoked Potentials, Auditory, Brain Stem* ; Humans