AIM　To study protective effect of taurine on diaphragm against adriamycim cytotoxity in rabbits.METHODS　The rabbits were divided randomly into three groups.①Control group:The animals were administered NS 2 ml*kg-1 for five days. ②Adriamycin+NS group:The animals were administered NS 2ml*kg-1 for five days and then administered adriamycin 10 mg*kg-1.③Adriamycin+taurine group:The animals were administered taurine 100 mg*kg-1 for five days and then administered adriamycin 10 mg*kg-1.After 24 h,three groups animals were anaesthetized.The transdiaphragmatic pressure(Pdi) and diaphragm evoked potential(DEP) were measured.SOD and MDA were tested.the ultrastructure of diaphragm cells was investigated by eletronmicroscopy.RESULTS　The Pdi and amplitude of DEP were lowered by adriamycin(P＜0.01).The levels of MDA was increased and the activity of SOD was decreased in diaphragm(P＜0.05).The disorder of myofibrills,swelling of mitochondria with broken cristase can be observed by electronmicroscopy.The above changes were inhibited by taurine.CONCLUSIONS　The taurine could scavenge the toxic radicals generated by adriamycin and protect the diaphragm myocytes.

AIM To study protective effect of taurine on diaphragm against adriamycim cytotoxity in rabbits. METHODS The rabbits were divided randomly into three groups. groups group: The animals were administered NS 2 ml.kg- 1 for five days. ②Adriamycin + NS group: The animals were administered NS 2ml. kg-1 for five days and then administered adriamycin 10 mg. kg- 1. ③Adriamycin + taurine group: The animals were administered taurine 100 mg. kg-1 for five days and then administered adriamycin 10 mg?kg- 1. After 24 h, three groups animals were anaesthetized. The transdiaphragmatic pressure(Pdi) and diaphragm evoked potential(DEP) were measured. an and MDA were tested. the ultrastructure of diaphragm cells was investigated by eletronmicroscopy. RESULTS The Pdi and amplitude of DEP were lowered by adriamrcin(P

To study the protective effect of Shengmai Injection on adriamycin-induced cytotoxity of diaphragm in rabbits. The rabbits were randomly allocated to three groups. The control group were treated with NS 2 ml?kg -1 for five days, adriamycin+NS group with NS 2ml?kg -1 for five days and then administered adriamycin 10 mg?kg -l and adriamycin + Shengmai Injection group with Shengmi Injection 2 ml?kg -l for five days and then administered adriamycin 10 mg?kg -1 . Twenty-four hours later, animals in the three groups were anaesthetized. Then the transdiaphragmatic pressure (Pdi) and diaphragm evoked potential(DEP) were measured, SOD activity and MDA content were tested and the ultrastructure of diaphragm cells was observed under electron microscope. Adriamycin could lower the Pdi and amplitude of DEP (P

Thyroid-stimulating hormone receptor(TSHR)is an important autoantigen whose cleavage and shedding can induce autoimmunity.The TSHR is the main functional receptor of the thyroid.The shedding of the ? subunit plays an important role in the forming of TSAb and balancing between TSAb and TBAb.Research on the cleavage and shedding of the TSHR extracellualar domain helps to gain a deep insight into the structure and function of TSHR,illuminate the pathogenesis of autoimmune thyroid disease and find out a new therapy.The structure of TSHR,the mechanism and the significance of its cleavage and shedding are reviewed in this article.

Objective To evaluate the optimal management of cardiac surgery during pregnancy,in the second or third trimester,and the maternal and fetal outcomes in pregnant patients after surgery.Methods Six pregnant women with heart diseases were identified,who underwent cardiac surgery with cardiopulmonary bypass.Surgery was performed using cardiopulmonary bypass at mild hypothermia/normothermia,with pulsatile perfusion.Natural progesterone(20 mg)was added in priming solution,monitoring uterine contractions and heart rate changes.The mode of surgeries included mitral valve replacement+tricuspid valve plastic in three cases,aortic and mitral valve replacement+tricuspid valve plastic in one,left ventricular myxomatomy in one,left ventricular septal myectomy+artificial aortic valvular vegetations dissection+tricuspid valve plastic in one.Results The cardiopulmonary bypass and cross clamp time averaged 169 minutes(range,96~419 minutes)and 113 minutes(range,56~296 minutes),respectively.Five patients were alive.One maternal and fetal death occurred 6 h after surgery caused by heart failure and pulmonary edema.The other 5 patients terminated pregnancies after heart surgeries,one underwent with cesarean section 3 d after surgery in second trimester,two of whom with induction of labor,and two full-term labor(one with cesarean section,one with normal delivery).Two newborns were alive with no malformation.Conclusion Cardiopulmonary bypass can be used safely with satisfactory maternal and fetal outcomes in pregnant patients with heart disease undergoing cardiac surgery.

Objective To observe the effects of gypenosides(GP) on cardiac dysfunction induced by adriamycin in rats and to investigate the therapeutic mechanism.Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups:control group,adriamycin(ADR) group,low-,moderate-and high-dose GP groups.The low-,moderate-and high-dose GP groups received GP 100,200 and 400 mg? kg-1? d-1 for five days respectively.Meanwhile,the purified water was given to rats of control group and adriamycin groups.On the sixth day,adriamycin(10 mg? kg-1) was given intraperitoneally(ip) to all the groups except the control rats.At the twenty-fourth hour after administration of adriamycin,the cardiac functions such as left ventricular systolic pressure(LVSP),maximal rates of increase and decrease of left ventricular pressure(? dp/dtmax),as well as the activities of cardiac ATPase,succinic dehydrogenase(SDH) and sarcoplasmic reticulum Ca2+-ATPase(SERCA2a) were measured.Results The impairment of heart function induced by adriamycin was relieved and the activities of cardiac ATPase,SDH and SERCA2a were obviously increased after GP treatment.Conclusion GP have protective effects on cardiac dysfunction induced by adriamycin in rats,and the mechanism may be related to the increase of activities of cardiac ATPase,SDH and SERCA2a.

Objective Using the soaking seedling method to introduce wild Gastrodia elata DNA into potato plantlets and analyze gastrodin of transformed Solanum tuberosum.Methods After the tuber grown up,the solution of gastrodin was extracted from the potatoes which were transformed by wild G.(elata) DNA.The transformed plants were scaned by ultraviolet.PCR was used to analyze GAFP gene by SDS-PAGE.TLC was used to analyze the gastrodin of transformed S.tuberosum.Results(1)The 21 transformed plants had obvious absorption peak at 220 nm in 200 transformed S.tuberosunr.(2)The fragment of wild G.elata GAFP gene was found by PCR from 5 transformed plants.(3)The protein expressions in transgenic S.tuberosum were obviously different from the normal S.tuberosum;a band was detected in transgenic S.tuberosum and wild G.elata,but wasn't detected in normal S.tuberosum.(4)The expression of the gastrodin was detected by TLC from three transformed plants. Conclusion It is feasible to use the soaking seedling method to introduce exogenous DNA into plants.

Objective To investigate the clinicopathologic features and the expression of p53,PCNA and Bcl-2in tricholemmal carcinoma.Methods Skin lesions were studied with HE staining and immunohis-tochemical markers for p53,PCNA and Bcl-2(S-P method)in22cases of skin tricholemmal carcinoma.Both morphological manifestations and immunohistochemical expression were observed.Results There were15fe-male and7male cases of tricholemmal carcinoma with the ages of40-79y,including16cases whose scalp were involved.Positive staining of p53,PCNA and Bcl-2was found in72%,100%and63%of patients,re-spectively.The positive cells of p53were diffusely distributed.PCNA-positive cells were increased along with the degree of differentiation.Significant differences of Bcl-2expression were found among the various degrees of differentiation(P

Antiinf lammatory effects of Ferulofen ( FL ) were shown by im or ip on xylene-induced swellingof mice ear, carrageenin produced edema of hind paw in rats, and decreased volume of pleural exud- ate on carrageenin-induced acute pleurisy of rats, FL also inhibi-tied the proliferating granuloma by croton oil. The above dose r-ange in 50-100mg/kg im or ip which presented a good dose-effect relationship, FL also inhibited the mice writhing caused by H AC & tail-flick response to 1 light irradiation, suggesting its strong analgesic effect. In addition, its anticancer effect in vitro ( carcinoma of Ehlrich ascitis)was better than that in vivo ( Sl80 sarcoma) in mouse. So FL may be considered to be a promising new type of non-steroidal antiinflammatory & anticancer drug ,worthy of further reseaching & exploring in the future.

Objective To explore the effects of oxidative stress in porcine iliac endothelial cells(PIECs) induced by high glucose. Methods After being intervened by high glucose for some time, dihydroethidium (DHE) or dihydrorhodamine 123 (DHR123) was used as a reactive oxygen species(ROS) capture. The mean fluorescent intensity(MFI) of above probes which were the products of intracellular oxidation was detected by fluorescent microscopy and flow cytometry, and the level of ROS was thus measured. With lucigenin as chemiluminescence agent, luminescence changes were detected by chemiluminescence analyzer after addition of NADPH to observe the effect of high glucose on activity of NADPH oxidase(NOX). Results Intracellular MFI was markedly elevated with the concentration of high glucose and time of exposure to high glucose. It was revealed by flow cytometry that the NOX activity was significantly activated compared with normal medium treated PIECs(P