1.Personality Characteristics of 1921 Cadets in an Air Force Academy Shown by the MBTI-G
Jing CHEN ; Dan-Min MIAO ; Yang WANG ;
Chinese Mental Health Journal 2002;0(11):-
Objective:To investigate influence of living locations on the personality characteristics of the only child and none-only children in an air force academy.Methods:1921 cadets were tested by the Chinese version of the MBTI-G.Results:Scores of the only child cadets from cities on the Sensing-Intuition and Thinking-Feeling dimensions were higher than those of none-only children group from cities and those of the only child group from countries.The scores of them on the SN scale were 93.0?18.5,90.4?17.6 and 90.1?17.7(t=2.25,P=0.025;t=1.99,P= 0.048).The scores of them on the TF scale were 94.6?22.6,89.1?21.1 and 88.4?21.0(t=4.02,P
2.Study on Photo-protective Mechanisms of Hydroxychloroquine and Epigallocatechin Gallat on Keratinocytes Damaged from Ultraviolet-B Irradiation
Wei MIN ; Dan LUO ; Xiangfei LIN ; Die WU ; Xu MIAO
Chinese Journal of Dermatology 2003;0(09):-
Objective To investigate the photo-protective mechanisms of hydroxychloroquine and epigallocatechin gallate (EGCG) on HaCaT cells damaged from UVB irradiation. Methods Subconfluent HaCaT cells were irradiated with different doses of UVB irradiation and treated with the above listed agents. The mRNA expression levels of p53, p21, c-fos and GADPH genes were evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Results UVB irradiation induced mRNA expression of p53, p21 and c-fos in cultured HaCaT cells, which were alleviated by hydroxychloroquine and EGCG treatment in UVB irradiation group. Conclusions The photo-protective effects of hydroxychloroquine and EGCG on HaCaT cells by UVB irradiation might be related to inhibition of the expression of p53,p21 and c-fos genes.
3.Cloning and expression analysis of GGPPS gene from Panax notoginseng.
Dan-dan MIN ; Mei-qiong TANG ; Gang LI ; Xiao-sheng QU ; Jian-hua MIAO
China Journal of Chinese Materia Medica 2015;40(11):2090-2095
According to the transcriptome dataset of Panax notoginseng, the key geranylgeranyl pyrophosphate synthase gene (GGPPS) in terpenoid backbone biosynthesis was selected to be cloned. Using specific primer pairs combining with RACE (rapid amplification of cDNA ends) technique, the full-length cDNA sequence with 1 203 bp, which containing a 1 035 bp open reading frame, was cloned and named as PnGGPPS. The corresponding full-length DNA sequence contained 2 370 bp, consisted of 1 intron and 2 exons. The deduced protein PnGGPPS contained 344 amino acids and shared more than 73% identity with GGPPS from Ricinus communis and Salvia miltiorrhiza. PnGGPPS also had specific Aspartic acid enrichment regions and other conserved domains, which belonged to the Isoprenoid-Biosyn-C1 superfamily. The quantitative real-time PCR showed that PnGGPPS expressed in different tissues of 1, 2, 3 years old root, stem, leaf and 3 years old flower, and the expression level in 3 years old leaf was significant higher than that in other organs, which suggested that it might not only be involved in the regulation of the growth and development, but also be associated with the biosynthesis of chlorophyll and carotenoids, the development of chloroplast, the shade habit and the quality formation of P. notoginseng.
Cloning, Molecular
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Computational Biology
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Geranylgeranyl-Diphosphate Geranylgeranyltransferase
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genetics
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Panax notoginseng
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genetics
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Real-Time Polymerase Chain Reaction
4.Effects of Imiquimod on the Secretion of Cytokines by Human Epidermal Langerhans Cells and Ha- CaT Cells
Xu MIAO ; Dan LUO ; Xi JI ; Lixian XU ; Wei MIN ; Xiangfei LIN ; Yang XU
Chinese Journal of Dermatology 2003;0(10):-
Objective To investigate the effects of imiquimod on cytokine secretions of normal human epidermal Langerhans cells (LCs) and HaCaT cells, and to better understand the mechanism of immune regulation by imiquimod. Methods LCs were sorted from prepared epidenmal cells by density gradient centrifugation and magnetic-activated cell sorting (MACS). LCs and HaCaT cells were cultured in media with or without 5 ?g/mL of imiquimod for 4 hours, then cell-free culture supernatants were harvested, cytokines were detected by ELISA kits. Results TNF-?, IL-1? and IL-6 secreted from imiquimod treated LCs were all higher than those from control LCs (all P
5.Serum lipocalin-2 is a potential biomarker for the clinical diagnosis of nonalcoholic steatohepatitis
Gang XU ; Yu-Min WANG ; Miao-Miao YING ; Sui-Dan CHEN ; Zong-Rui LI ; Hong-Lei MA ; Ming-Hua ZHENG ; Jian WU ; Chunming DING
Clinical and Molecular Hepatology 2021;27(2):329-345
Background/Aims:
Nonalcoholic steatohepatitis (NASH) is a progressive form of nonalcoholic fatty liver disease (NAFLD) characterized by hepatic steatosis, inflammation, hepatocellular injury, and fibrosis. We aimed to investigate the usefulness of a key biomarker, lipocalin-2 (LCN2), for the detection of NASH progression.
Methods:
A mouse NASH model was established using a high-fat diet and a high-sugar drinking water. Gene expression profile of the NASH model was analyzed using RNA sequencing. Moreover, 360 NAFLD patients (steatosis, 83; NASH, 277), 40 healthy individuals, and 87 patients with alcoholic fatty liver disease were recruited.
Results:
Inflammatory infiltration, focal necrosis in the leaflets, steatosis, and fibrosis were documented in the mouse liver. In total, 504 genes were differentially expressed in the livers of NASH mice, and showed significant functional enrichment in the inflammation-related category. Upregulated liver LCN2 was found to be significantly interactive with various interleukins and toll-like receptors. Serum LCN2 levels were significantly increased in NAFLD patients. Serum LCN2 levels were correlated with steatosis, intralobular inflammation, semiquantitative fibrosis score, and nonalcoholic fatty liver disease activity score. The area under the curve of serum LCN2 was 0.987 with a specificity of 100% and a sensitivity of 93.5% for NASH diagnosis, and 0.977 with almost the same specificity and sensitivity for steatosis.
Conclusions
LCN2 might be involved in the transition from NAFL to NASH by mediating inflammation. Serum LCN2 levels might be a novel biomarker for the diagnosis of NASH.
6.Serum lipocalin-2 is a potential biomarker for the clinical diagnosis of nonalcoholic steatohepatitis
Gang XU ; Yu-Min WANG ; Miao-Miao YING ; Sui-Dan CHEN ; Zong-Rui LI ; Hong-Lei MA ; Ming-Hua ZHENG ; Jian WU ; Chunming DING
Clinical and Molecular Hepatology 2021;27(2):329-345
Background/Aims:
Nonalcoholic steatohepatitis (NASH) is a progressive form of nonalcoholic fatty liver disease (NAFLD) characterized by hepatic steatosis, inflammation, hepatocellular injury, and fibrosis. We aimed to investigate the usefulness of a key biomarker, lipocalin-2 (LCN2), for the detection of NASH progression.
Methods:
A mouse NASH model was established using a high-fat diet and a high-sugar drinking water. Gene expression profile of the NASH model was analyzed using RNA sequencing. Moreover, 360 NAFLD patients (steatosis, 83; NASH, 277), 40 healthy individuals, and 87 patients with alcoholic fatty liver disease were recruited.
Results:
Inflammatory infiltration, focal necrosis in the leaflets, steatosis, and fibrosis were documented in the mouse liver. In total, 504 genes were differentially expressed in the livers of NASH mice, and showed significant functional enrichment in the inflammation-related category. Upregulated liver LCN2 was found to be significantly interactive with various interleukins and toll-like receptors. Serum LCN2 levels were significantly increased in NAFLD patients. Serum LCN2 levels were correlated with steatosis, intralobular inflammation, semiquantitative fibrosis score, and nonalcoholic fatty liver disease activity score. The area under the curve of serum LCN2 was 0.987 with a specificity of 100% and a sensitivity of 93.5% for NASH diagnosis, and 0.977 with almost the same specificity and sensitivity for steatosis.
Conclusions
LCN2 might be involved in the transition from NAFL to NASH by mediating inflammation. Serum LCN2 levels might be a novel biomarker for the diagnosis of NASH.
7.Clinical analysis on the mobilization and collection of peripheral hematopoietic stem cells in 198 healthy donors
Si-yong HUANG ; Li LIU ; Miao-wang HA0 ; Hua HE ; Zhen GU ; Renan CHEN ; Wei-wei QIN ; Yan-lan WU ; Guo-hui LI ; Dan-dan YIN ; Ying-min LIANG
Journal of Leukemia & Lymphoma 2011;20(9):522-524
ObjectiveTo investigate the methods and effects on the mobilization and collection of peripheral hematopoietic stem cells.MethodsPeripheral hematopoietic stem cells of 198 cases of healthy donors were selected and mobilized by subcutaneous recombinant human granulocyte colony stimulating factor (rhG-CSF) by (5-10) μg·kg-1 ·d-1 and collected on the 5th day. The effects of gender, height, age and WBC count of donors on mobilization and collection were analyzed. ResultsAll the peripheral hematopoietic stem cells of donors were successfully mobilized.The average counts of mononuclear cells(MNC)and CD34+ cells were (4.19±1.96)×108/kg and (2.98±1.40)×106/kg, respectively, which had no correlation with gender, height and age.The counts of MNC and CD34+ cells collected were positively correlated with the WBC count of peripheral blood (r =0.9201, P =0.0035; r =0.8420, P =0.0149). The donors with WBC ≥20.0×109/L had moresignificant effect than thoseWBC<20.0×109/L (F =4.688, P =0.0013; F =4.622, P =0.0006). Conclusion The WBC count of peripheral blood from healthy donors is a simple and feasible indicator to predict the quantity of CD34+ cells.
8.Clinical analysis of peripheral blood stem cell mobilization regimens in autologous transplantation for treating non-Hodgkin's lymphoma.
Si-Yong HUANG ; Li LIU ; Miao-Wang HAO ; Dan-Dan YIN ; Yan-Lan WU ; Ren-An CHEN ; Gou-Hui LI ; Qian LUI ; Jing-Cheng WANG ; Hua HE ; Ying-Min LIANG
Journal of Experimental Hematology 2011;19(6):1415-1418
The purpose of this study was to compare the efficacy of CEP plus G-CSF and CVP plus G-CSF regimens in the mobilization and collection of peripheral blood hematopoietic stem cells (PBHSC), and in the hematopoietic recovery. 57 patients with non-Hodgkin's lymphoma (NHL) underwent autologous PBHSC transplantation were analyzed retrospectively. The PBHSC were mobilized and collected by using CEP plus G-CSF and CVP plus G-CSF respectively, and were retransfused into these NHL patients after preconditioning, then the mobilization efficacy, adverse reactions and hematopoietic recovery were analyzed. The results showed that the WBC count decreased to ≤ 1.0 × 10(9)/L, platelet amount dropped to ≤ 40 × 10(9)/L during peripheral blood stem cell mobilization of all patients, which indicated successful collection of PBHSC. The mean value of (4.38 ± 3.40) × 10(8)/kg mononuclear cells (MNC) containing (2.79 ± 2.53) × 10(6)/kg CD34(+) cells were collected in CEP plus G-CSF group, while the mean value of (3.31 ± 1.23) × 10(8)/kg MNC containing (2.02 ± 0.87) × 10(6)/kg CD34(+) cells were collected in CVP plus G-CSF group. The efficacy of mobilization in CEP plus G-CSF group was significantly higher than that in CVP plus G-CSF group (p < 0.05). After preconditioning, bone marrow was suppressed in all patients. The average time of WBC count recovery to ≥ 1.0 × 10(9)/L was 11.4 days in CEP plus G-CSF group and 12.3 days in CVP plus G-CSF group; the average time of platelet amount recovery to ≥ 50 × 10(9)/L was 18.6 days in CEP plus G-CSF group and 19.3 days in CVP plus G-CSF group. The statistical analysis showed no significant difference in the average time of hematopoietic recovery between 2 groups. It is concluded that autologous PBHSC transplantation shows significant effect for treatment of patients with NHL. Either modified CEP or CVP plus G-CSF regimen is safe and effective in PBHSC mobilization. The CEP plus G-CSF regimen is better than CVP plus G-CSF regimen.
Adolescent
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Adult
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Child
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Female
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Hematopoietic Stem Cell Mobilization
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methods
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Humans
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Lymphoma, Non-Hodgkin
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therapy
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Male
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Middle Aged
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Peripheral Blood Stem Cell Transplantation
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Retrospective Studies
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Transplantation, Autologous
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Young Adult
9.Repeated fever with cytopenia.
Lei GAO ; Yu Jie WANG ; Miao Xia HE ; Gu Sheng TANG ; Xiao Xia HU ; Dan YANG ; Jian Min WANG ; Jian Min YANG
Chinese Journal of Hematology 2019;40(11):962-964
Anemia
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Fever
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Humans
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Leukopenia
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Thrombocytopenia
10.Intention survey of target population with regard to H1N1 influenza vaccination.
Min LU ; Luo-dan SUO ; Li LU ; Liang MIAO ; Wen-yan JI ; Dong-lei LIU ; Rui MA ; Yu-chan HUANG ; Jiang WU
Chinese Journal of Epidemiology 2010;31(4):477-478