No abstract available.
Animals ; Female ; Follicular Fluid* ; Humans* ; Mice* ; Oocytes*

No abstract available.
Female ; Lactation* ; Pregnancy*

No abstract available.
Female ; Lactation* ; Pregnancy*

Objective To investigate the effects of macmphage migration inhibitory factor (MIF) on glucocorticoid (GC) re]ease and glucocorticoid recer (GR) in mts.Methods Test Ⅰ Thirty-two male SD rats weighing 250-300 g were randomly divided into 4 groups(n=8 each):control group(C),low dose recombinant MIF (rMIF) group (rMIF-L),middle dose rMIF group (rMIF-M) and high dose rMIF group (rMIF-H).The animals received l ml normal saline via the right femoral vein in group C.The animals received rMIF50.100 and 200 ng in l ml normal saline though right femoral vein in group rMIF-L,rMIF-M or rMIF-H respectively.Blood samples were taken from left femoral artery immediately before inection(T0,baseline),and at 5 min,3 h,6 h.12 h and 24 h after injection of rMIF(T1-5) for determination of serum concentration of corticosterone.Test Ⅱ Primary cultured neonate rat(2-3 days)myocardial ceils were randomly divided into 3 groups(n=24 each):group C,group rMIF-L and group rMIF-M.The ceUs in group C,rMIF-L and rMIF-M wefe incubated with DMEM.rMIF 50 ng+DMEM and rMIF 100 ng+DMEM for 3 h respectively.The expression of GR and HsPg0 wag determined by Western blot.ResuBs Test Ⅰ The serum concentration of corticosterone was signifieemily higher in the other 3 groups than in group C at T1-5(P<0.05).The sertlm concentration of corticostemne was significantly increased at T1-5 in group rMIF-L,rMIF-M and rMIF-H compared with the baseline values(P<0.05).Test Ⅱ HSP90 expresion was significantly lower in the other two groups than in group C(P<0.05).Them was rio signifieanf difference in HSP90 expression between group rMIF-L and group rMIF-M(P>0.05).There was no significant difference in GR expression among the 3 groups ( P > 0.05). Conclusion MIF druing sepsis can weaken GR function through down-regulating HSP9O expression, resulting in CC resistance.

Objective To analyze the clinical treatment and curative effect of heart failure complicated with hyponatremia in elderly patients.Methods The clinical data were retrospectively analyzed in 72 aged patients with heart failure and hyponatremia,by controlling the intake of fluid and using diuretics and the drugs of heart failure,not restrict a low sodium diet,by adding sodium chloride,analyzed the effect and prognosis after moderate supplement of sodium for 7 days.Results The use of loop diuretics reduced cardiac edema,renew and sodium chloride without aggravating heart failure or hypernatremia,total effective rate was 95.83％,and it was safe and effective.Conclusion For the elderly patients with heart failure complicated with hyponatremia,should be based on the conventional symptomatic treatment of heart failure,correction of hyponatremia,improve cardiac function and prognosis of patients.

No abstract available.
CA-125 Antigen* ; Humans ; Ovarian Neoplasms*

By using digoxin labelled human papillomavirus(HPV) 6/11 and HPV16/18 probes,and hybridization in situ technique, the HPV DNA sequence in 50 cases of oral SCC was detected. The results showed that among them ,16 cases(32%) were positive for HPV16/18 DNA, none of the cases of OSCC was positive for HPV6/11 DNA. It was suggested that HPV16/18 in oral SCC was confirmed to be closely related with the cause of OSCC.

Nitric oxide ( NO ) is now recognized as a mediator of several biological and immunological functions, but unlike classical neurotransmitters, NO simply diffuse of the postsynaptic cell and around affecting cells. Human chorionic gonadotropin ( hCG ), produced by placental trophoblasts may act as stimulator on NO synthesis in oocytes of mouse's ovary. How-ever, in the various organs or cells, the action of hCG on NO synthesis is unknown. We have examined that the effect of hCG on NO synthesis in microglial cells of murine's brain, using the Griess method. And this study was evident that hCG did not induce NO produc-tion without recombinant interferon gamma ( rIFN-gamma), whereas hCG ( 10~500 IU/ml ) with rIFN-gamma effectively produced NO in microglial cells of brain. As result, NO production in microglial cells increased most significantly in dose of 100 IU/ml of the hCG and the pro-duction of NO was dependent on the dose of hCG ( Table 1 and Fig. 1 ). And N(G)-monomethyl-L-arginine ( N(G)MMA ), competitive inhibitor of NO synthase, reduced the NO production by hCG stimulation with rIFN-gamma in microglial cells of murine. Conclusively, this study sugge-sted that hCG stimulate NO production at microglial cells in brain, which may be an important factor for mediating immune and neuroendocrinologic regulation in nervous system.
Brain ; Chorionic Gonadotropin* ; Female ; Humans* ; Interferons ; Negotiating ; Nervous System ; Neurotransmitter Agents ; Nitric Oxide Synthase ; Nitric Oxide* ; Oocytes ; Ovary ; Trophoblasts

The hydrophobicity assay and DNA probe hybridization assay were compared for analysis of enterotoxigenic Escherichia coli(ETEC), heat-labile enterotoxin(LT) and heat-stable enterotoxin (ST). The ETEC isolated from diarrheal patients were serotyped and investigated for the presence of colonization factor antigens CFA/1, CFA/II, CFA/III and CFA/IV with the expression of mannose-resistant hemagglutination(MRHA) and the levels of surface hydrophobicity. The following results were obtained. 1. Out of these 48 strains, 34 strains were found to be positive for LT production by DNA probe hybridization assay. Out of 34 strains, 1 strain was ST producer, 25 strains were LT producers, and 8 strains were produced both ST+LT producers by DNA probe hybridization assay. 2. Out of 34 strains of positive DNA probe hybridization test, 31 strains was positive in the hydrophobicity test. Among strains of positive hydrophobicity test, 20, 1, and 7 strains produced only LT, only ST and both ST-LT, respectively. Screening efficiency for identifying ETEC by salting out test was 82.4% in sensitivity and 78.6% in specificity. For ETEC detection, the hydrophobicity assay was the least sensitive but was simple, rapid and a good substitute for the DNA probe hybridization assay. 4. CFAs were identified in 43.8% of ETEC strains; 2.1% of the CFAs strains with CFAs harbored CFA/I, 29.2% carried CFA/II, 16.7% carried CFA/III and CFA/IV. And 35.4% expressed none of these CFAs. CFA/I was found in ETEC of serotype 0128: K67, CFA/II was 0128: K67, 0142: K+ and 0159: K+, CFA/III was 086a: K15 and 0128: K67, CFA/IV was 0 86a: K15, 0128: K67, 0125: K70 and 0148: K+.
Colon ; DNA* ; Enterotoxigenic Escherichia coli* ; Enterotoxins ; Escherichia ; Humans ; Hydrophobic and Hydrophilic Interactions* ; Mass Screening ; Sensitivity and Specificity

Eighty-two strains of Escherichia coli isolated from urine specimens in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profiles, PFGE profiles, MRHA of human blood cells, HEp-2 cell adherence ability and reactivity to bfpA, LT, STh and STp DNA probes. The following results were obtained. Fifty-three of the eighty-two strains belonged to thirteen different 0 serotypes, twenty-nine strains could not be typed with the antisera used. Thirty strains (43.9%) were hemolysin producer. MRHA is present on twenty-nine strains (35.37%) of eighty-two strains. MRHA positive strains carry a plasmid of 60MDa, a putative factor involved in adherence. This plasmid might be specific for MRHA positive strains. MRHA positive strains were observed in serotype 01, 018, 055, 086a, 0119, 0126, and 0142. Twenty-six strains of E. coli showed three patterns of adherence to HEp-2 cells namely, localized, diffuse, and aggregative adhesion. Twenty-two strains hybridized with the bfpA probe, while all eighty-two strains did not hybridize with the probes, LT, STh, STp. The restriction fragment patterns of chromosomal DNA digested with AotI analysed by PFGE of hemolysin-producing E. coli ten strains were compared with eight different types. Three of E. coli serotype 01, 08 and 0126 showed the same chromosomal DNA fragment patterns.
Blood Cells ; Busan ; DNA ; DNA Probes ; Escherichia coli* ; Escherichia* ; Humans ; Immune Sera ; Plasmids ; Serotyping*