Object To provide an economical and practical technique for planting of Radix Gentianae in large scale Methods Indoor artificial pregermination, insemination in carboxymethyl cellulose suspension, irrigation with micro sprayer and no shelter growing technique of seedling Results The seedling cost is 60% lower than that of conventional technique In each square meter 3 000 seedlings were grown Conclusion This technique can be widely applied.

Object To investigate the reason of the low germination and sprout rates by studying on inhibitory activities of the crude extracts of the seed of the dried root of Saposhnikovia divaricata (Turcz.) Schischk (DRSD) on Brassica, wheat and the seed of DRSD. Methods The ether extract and residue of the crude extract were prepared and treated by paper chromatography. The inhibitory activities of different fractions with different Rf value were determined. Effect of steeping the seed in warm water at 41 ℃ and 45 ℃ for different periods of time was also studied, respectively. Results There are intrinsic inhibitor in the seed of DRSD with strong activity. The portion of its ether extract with Rf 0.6 showed the strongest inhibitory activity for the germination of Brassica seed. The crude extract of the seed of DRSD also has the stronger inhibitory activity to the germination and growth of tender root of S. divaricata and has the same effect on aerial part of wheat sprouts. Steeping with warm water and solvent can remove most of the intrinsic inhibitor. Conclusion Intrinsic inhibitor in the seed of DRSD is the main factor that results in the low germination and sprouting slowly after seeding.

Semaphorins is the one of guidance molecule families.It is proved to have wide physiological function in the development of neutron and play important role in the pathogenesis of retinal degenerative diseases,gangliocyte apoptosis-related diseases and neovascularization.Previous studies showed that Semaphorins distribute in the cornea,lens and retina.Semaphorins play important role in the development of retina,visual system,cornea nerve and reduce the death of retinal ganglion cells(RGCs)caused by glaucoma,retinal detachment and hypoxic ischemic optic neuropathy.Semaphorin family-related gene mutation may be associated with retinal degenerative diseases.Some of the members could also inhibit angiogenesis.Since semaphorins and its receptors participate in the development of the vasculature and nervous systems,they are implied to be the potential new target of ophthalmopathic treatment.The current ptogress in structure and functions of the semaphorin family along with their relationship with ophthalmopathy were summarized in this study.

10 adult female rats were used to study blast injuries of the air-way and lung. The conclusions are as follows. 1. There was significant difference in arterial blood gas analysis between the normal control and injury group. 2. The une-venness in the lung damage, bleeding, tear of the alveolar septa induced by the air shock wave was predominant, by scanning electron microscopy (SEM) as well as by the light microscopy (LM) . The bleeding may be due to the capillary rupture of alveolar septa. The' rope-like' structures seen might represent the fibre component in the septa which had more resistance to the local force and remained after the other components were wiped away by explosion. 3. Striping and fissure of the epithelium of trachea and bronchi of the injured group were detected. 4. The pathological changes observed with SEM were compared to those with LM.

The morphological changes of the rat lung with blast injury are quantitified with a stereological method. After blast the pulmonary capillary volume and internal surface area decreased significantly, arithrnatic and harmonic mean thickness of the air-blood barrier, the volumes of P'MN and platelet in capillury increased remarkably. The morphometric lung diffusion capacity of oxygen (DLO2) is reduced markedly mainly due to the decrease of diffusion capacity of air-blood barrier and erythrocyte. The change of DLO2 is closely correlated with the arterial blood oxygen pressure, indicated that the damage of pulmonary capillary is one of the main causes of alveolar bleeding and the early respiratory insufficiency. A mathematic relation can be established between the intensity of the blast wave and the corresponding morphological changes of the injured lungs.

Objective To explore the serum lactic acid,transaminase and their relationship with liver pathological damage in sepsis rats,whether a correlation exists between serum lactate clearance rate and transaminase in sepsis rats,and whether they can be used as indicators for the prediction and evaluation of septic rats liver injury.Methods A total of 150 clean Sprague-Dawley (SD) rats was divided into normal group (n =50),sham operated group (n =50),and the cecal ligation and puncture (CLP) (n =50).Ten rats were killed after successful surgery at the 6th,12th,24th,48th,and 72nd hour in CLP group,respectively.At each time point,10 normal rats and 10 sham-operated rats were taken as a control in the corresponding time point.The liver tissue was obtained for pathological analysis.The levels of lactate and liver transaminase were detected and the lactate clearance rate was calculated.The levels of lactate and transaminase at each time point were statistically compared.The correlation analysis was performed among serum lactate,transaminase,and liver damage pathological changes.Results The rat hepatocytes in CLP group begin to appear at the 6th hour,and the damage was gradually enhanced at the 12th,24th,and 48th,and up to the worst damage at the 48th time point.The levels of lactate and alanine aminotransferase (ALT)in CLP group at the 12th and 24th hour were significantly elevated compared with the normal and sham operation groups (P ＜0.05); The level of aspartate aminotransferase (AST) in CLP group at the 6th,12th,24th,48th,and 72nd hour was significantly elevated compared with the normal and sham operation groups (P ＜ 0.05).A positive correlation was found between the levels of ALT and lactate (r =0.766,P ＜0.05),and a negative correlation was found between lactate clearance rate and ALT (r =-0.712,P ＜0.05).Conclusions In septic rats,both lactate and lactate clearance rate were correlated with ALT,and they were correlated with liver pathological damage.The level of lactate,lactate clearance rate,and ALT could be used as the key indicators to predict liver damage in septic rats.

?AIM: To investigate the compliance in patients with topical glaucoma therapy, and identify the most frequent barriers to adherence.?METHODS: One-on-one interview and questionnaire were taken to investigate the compliance with hypotensive eye drops in 366 patients who had been diagnosed as glaucoma and received the therapy for at least 6mo in Wuhan and suburban district from July 2014 to June 2015 in Wuhan Aier Eye Hospital.?RESULTS: The proportion of compliance was 44. 3%( 162/366 ) . Factors significantly influencing compliance included severity of the better eye, the severity of symptom when attacked, duration of medication and whether to administrate on the same time daily ( OR=5. 700,0. 554,0. 336,0. 558,P<0. 05). Age, gender, marital status, education level, economic status, domicile, knowledge of the disease, suffering from other diseases with medication, type of glaucoma, type of eye drop, frequency were not associated with a higher rate of non-compliance.? CONCLUSION: Compliance in patients with topical glaucoma therapy in Wuhan is poor. Regularly carrying out relevant health education and interventions is very important.

Abstract?AIM: To assess the efficacy and adverse reactions of two concentrations of diluted botulism toxin A ( BTX-A) for blepharospasm.?METHODS:Totally 220 patients (440 eyes) confirmedas blepharospasm in our department ( from January 2013 to January 2015 ) , were divided randomly into two groups:110 in high concentration group and 110 in low concentration group.The two groups were given local injections with 25 U/mL or 40 U/mL concentration of botulism toxin type A to the upper and lower eyelids orbicular muscle, corrugator, reducing eyebrow muscle and procerus respectively.We observed dosage of two groups, the treatment effect, the duration of the effect and the incidence of adverse reactions.?RESULTS: The high concentration group was given the BTX-A with 48.33 ±4.02U, the low concentration group was given the BTX-A with 28.51 ±3.42U,the differences were statistically significant( P<0.05).The differences of post-operative evaluation of curative effect, onset time and duration of the effect between the groups were not statistically significant ( P >0.05 ). Sixteen cases had ptosis in different levels in the high concentration group, and 2 in the low concentration group, the differences were statistically significant ( P<0.01).There were no serious complications such as intoxication, drug allergy in both groups.Ptosis relieved after using naphcon-A and disappeared in 2-3 wk.?CONCLUSION: The efficacy of local injection with two concentrations of diluted botulinum toxin A for blepharospasm was positive. Considering the high incidence of ptosis in high concentration group, we recommended the 25U/mL botulism toxin A for blepharospasm.

Object An attempt to seek after an intrinsic inhibitor present in the seed of Astragalus membranaceus (Fisch.) Bge.. Methods Crude ethereal extract of the seed was prepared and treated on paper chromatography. Inhibitory effects of different fractions with different Rf value were tested on Brassica chinensis L. and wheat germination. Effect of steeping the seed in warm water at 41 ℃ or 45 ℃ for different periods of time was also studied.Results Seed of A. membranaceus does contain strong intrinsic inhibitor. The portion of its ethereal extract with Rf 0.9 showed the most strong inhibition for the germination of Brassica, and the fraction with Rf 1.0 can inhibit the growth of the tender Brassica root, steeping with warm water can remove most of the intrinsic inhibitor, which also inhibits the growth of both aerial and underground parts of wheat sprouts, but without effect on its seed germination. It also showed strong inhibition of seed germination and growth of tender root of A. membranaceus. Conclusion Besides the low water permeability of the seed peel, the intrinsic inhibitor present in A. membranaceus is another essential factor that retard its germination.

OBJECTIVE To improve the administrative and controlling quality of hospital acquired infection(HAI),practice all kinds of works of HAI and decrease the incidence rate of it.METHODS We should strengthen leading power,perfect constitution and administration web,make project workable,intensify education of HAI,improve all medical staff administrative consciousness and whole quality of professional people of HAI,reinforce precaution and manipulation,and erect system of informational feedback.RESULTS All kinds of works of HAI were improved and medical staff administrative consciousness of HAI was enhanced through administration,and we could ensure the effects of sterilizing and killing bacteria.CONCLUSIONS We could carry out all kinds of institutions of HAI through administrative system of standard-check-reward and punish.