Objective:To evaluae the unification of endoplasmic reticulum and mitochondrium in fetal rat cerebral neuron apoptosis induced by intrauterine distress. Methods:Fetal rat intrauterine distress model was established. The samples were randomly divided into normal group and ischemia-reperfusion group(IR). Expression of GRP78,caspase-9,caspase-12,and cytoron C protein were detected by western blot technique. Results:The expression of GRP78 on corresponding time in IR group was significantly higher than that in normal group. And it reached the maximum at 3h of reperfusion. The expression of GRP78 reduced step by step as reperfusion time extended. The expression of caspase-9 and caspase-12 at corresponding time in IR group were significantly higher than those in normal group. Their expression increased rapidly and reached the maximum at 12h of reperfusion. The expression of cytoron C in kytoplasm at corresponding time in IR group was significantly higher than that in normal group and reached the maximum at 12h of reperfusion. Conclusion:The synergistic effect of endoplasmic reticulum and mitochondrium plays an important role in fetal rat cerebral neuron apoptosis induced by intrauterine distress.

Objective To investigate the mechanism of Thapsigargin in the proliferation of SW480 and provide experimental basis for development of anti-signaling therapy for patients with colon cancer by detection of intracellular calcium concentration in Thapsigargininduced human colon cell line SW480,.Methods Level of[Ca2+]was measured by fluorescence measurement with fura-2/AM,a fluorescent index of calcium.The suppression rate of SW480 cells was calculated by MTY assay.Results Thapsigargin promoted the levels of [Ca2+]from(133.26±5.17,197.46±4.62)to(436.63±5.21,766.41±3.12),and increased the suppression rate from(10.67±5.28)to(31.46±7.21).When W7 Was combined used with Thapsigargin,no significant changes were found in the levels of[Ca2+]and increment of the suppression rate.Condusion Thapsigargin might promote the proliferation of SW480 cells and lead to apoptosis.but the signaling pathway still remain to he studied.

Streptococcus pneumoniae is one of the most common causes of respiratory tract infections in children, and also the major pathogen leading to severe pneumonia, complications and even death. In recent years, antimicrobial resistance of streptococcus pneumoniae is growing and widespread, resulting in the increasing burden of disease,and also bring serious challenges to clinical diagnosis and treatment. The emergence and dissemination of vaccines against Streptococcus pneumoniae in disease prevention and control has a bright future.

In the process of the information literacy education, teachers, students and the society often have the conflict goals.Unity of purpose-based medical infornation literacy education research and emphasis on the decomposition of the different targets mean to extract the most important core object, so as to achieve social goals, teaching objectives and students target the greatest degree of uniformity, to achieve the maximization of the function of the information literacy education.

Objective To assess the quality of life and its influencing factors in ellerly patients with advanced cancer.Methods The quality of life was evaluated with EORTC QLQ-C30 scale Chinese version in 103 elderly patients with advanced cancer in a hospital outpatient clinic of Beijing from July 2013 to July 2014.The overall health,physical function,role function,cognitive function,emotional function,social function and individual symptoms score were assessed.The general information and clinical data of patients were analyzed.Results The general score of quality of life in elderly advanced cancer patients was (35.77 ± 21.98).In the domain of function the role functional score was the lowest (36.60 ± 27.97);in the domain of symptoms the tired score was the highest(50.82 ± 30.16),the score of appetite loss was the highest in individual symptoms (39.78 ± 24.32).Educational level,marital status,living condition,medical insurance and the number of comorbidities were correlated with overall health in elderly patients with advanced cancer (P ＜ 0.05).Multiple linear regression analysis showed that the educational level (t =2.447,P ＜ 0.05),marital status (t =2.118,P ＜ 0.05),medical insurance (t =3.770,P ＜ 0.05) and overall health status were positively correlated with the quality of life scores;and number of eomorbidities (t =-7.157,P ＜ 0.05) was negatively associated with the overall quality of life.Conclusion The quality of life was generally poor in elderly patients with advanced cancer;and educational level,marital status,medical insurance,comorbidities are important influencing factors.

Posterior scleritis,an inflammatory disease of the posterior sclera,is most commonly seen in middle-aged women and often associated with systemic immunological diseases.It presents with a variety of nonspecific signs and symptoms,and its clinical feature may be confused with ocular tumors,orbital inflammation,and retinochoroidopathy.Therefore,some auxiliary examinations are necessary for the diagnosis of posterior scleritis.With early proper diagnosis and treatment,severe complications of posterior scleritis can be avoided or lessened.In the paper,some advancement in the study of etiology,incidence,signs,symptoms,auxiliary examination,differential diagnosis and treatment of posterior scleritis were reviewed.

Objective To investigate the clinical efficacy of gemcitabine in the treatment of elderly patients with advanced non-small cell lung cancer.Methods 50 patients in our hospital from January 2014 to September 2015 were selected as the research object, and were randomly divided into the control group and the experimental group, each group of 25 patients each.The patients in the control group were treated with special therapy, and the patients in the experimental group were treated with gemcitabine.The clinical indexes of the experimental group and the control group were compared and analyzed.Results After the treatment, the experimental group of 25 patients, 12 cases of patients with complete remission, 8 cases partial remission, 3 cases were stable, 2 patients progressed, the effective rate was 75%, clinical benefit rate of 92%.In the control group, 4 patients had complete remission, 2 patients had partial remission, 9 patients were stable, 10 patients progressed, the treatment efficiency was 24%, and the clinical benefit rate was 60%.The effective rate and the clinical benefit rate of the experimental group were significantly higher than that of the control group (P<0.05).1 years later, the survival rate of patients in the experimental group was 22, the survival rate was 88%, and the number of patients in the control group was 14.The survival rate of the experimental group was significantly higher than that of the control group, with statistical difference (P<0.05).Conclusion Gemcitabine monotherapy in the treatment of elderly patients with advanced non-small cell lung cancer can improve the therapeutic effect to a great extent, good safety, low incidence of adverse reactions, high survival rate, with further clinical promotion and application significance.

Pertussis or whooping cough is an acute infectious disease of the respiratory system,which is mainly caused by Bordetella pertussis and Bordetella parapertussis. It can occur at any age,but is most serious in young infants. Despite widespread use of vaccination, pertussis has not been eliminated. On the contrary, increased incidence rates have been reported worldwide during the last two decades,also called reemergence of pertussis. So it is necessary to evaluate current state on pertussis research,in order to better provide basis for mo-nitoring and control pertussis. This paper reviews the pertussis epidemiological changes and the related cause analysis and immunization strategy.

Objective: Our aim was to investigate the relationship between tumor doubling time (TD) and the prognosis of patients with lung cancer.Methods:According to the diameter of tumor or its shadow,the TD of tumor was calculated and postoperative survival time (PST) were predicted based on the Geddes' nomogram.The statistic analysis was performed after comparison with the actual survival time (AST).Results:There was no significant difference of TD among tumors of different histological types (P>0.05).The accumulated survival in patients with TD>100 days was higher than those with TD<100 days, with a significant difference (P<0.05).The AST in patients underwent surgery was markedly higher than the PST,indicating that the operation was effective.Conclusion:Tumor TD may be a valuable parameter for evaluating the prognosis and surgical treatment in patients with lung cancer.