1.Isolation of a Specific Antigen from the Triton X-100 Soluble Protein (TSP) Of Mycobacterium tuberculosis.
Tae Hyun PAIK ; Hae Sook KWON ; Sun LEE ; Ji Sook LEE ; Eun Kyung JO ; Hwa Jung KIM ; Milllina LEE ; Yung Choon YOO ; Jung Kyu PARK
Journal of Bacteriology and Virology 2004;34(4):273-282
Tremendous efforts have been made to develop better vaccines and diagnostic markers for the effective control of tuberculosis. Recently, we reported that the Triton X-100 soluble protein (TSP) of Mycobacterium tuberculosis induced strong T-cell proliferation and IFN-gamma production in humans, and also conferred a significant level of protection against tuberculosis in a mouse model. In this study, the TSP was prepared by Triton X-100 extraction of Mycobacterium tuberculosis bacilli, which was followed by Triton X-114 phase partitioning. Western blot analysis using sera of 177 active pulmonary tuberculosis patients and 323 healthy individuals revealed that the TSP contained a immunodominant 40-kDa antigen specifically reacting with some sera from pulmonary tuberculosis patients. The 40-kDa antigen was purified by ion-exchange chromatography, and partially characterized by two-dimensional gel electrophoresis and N-terminal sequencing. Results of this study suggest that 40-kDa molecule of the TSP antigen from the cell suface of Mycobacterium tuberculosis can be used as a serodiagnostic marker as well as a potential vaccine candidate against tuberculosis.
Animals
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Blotting, Western
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Chromatography, Ion Exchange
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Mice
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Mycobacterium tuberculosis*
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Mycobacterium*
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Neptune*
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Octoxynol*
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T-Lymphocytes
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Tuberculosis
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Tuberculosis, Pulmonary
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Vaccines