Well-differentiated papillary mesothelioma (WDPM) of the peritoneum is considered to be a distinct subtype of peritoneal mesothelioma and has mostly behaved in a benign fashion. We report a case of WDPM in a 48-year-old-woman. It was incidentally found during a hysterectomy for a uterine cervical carcinoma. Grossly, the tumor was composed of multiple peritoneal nodules, measuring up to 2 cm. Microscopically, the nodules showed well-developed papillae lined by a single layer of cuboidal mesothelial cells. Immunohistochemical staining revealed a positive reaction for cytokeratin and a negative reacion for carcinoembryonic antigen and Leu-M1. Ultrastructurally, the tumor cells showed numerous long, slender microvilli and desmosomes.
Carcinoembryonic Antigen ; Desmosomes ; Hysterectomy ; Keratins ; Mesothelioma* ; Microvilli ; Peritoneum*

The search for a less traumatic method of cataract removal through a small incision, is on going. One of the advantages of phacoemulsification is that it permits the removal of a cataract through a small incision than otherwise possible. The small incision allows the refractive error to be stabilized sooner and reduces the amount of induced postoperative astigmatism. But it may be traumatic to the endothelium, especially in cases with hard nucleus. There is a method using the Nd:YAG laser to preoperatively soften the nucleus of the cataract intracapsularly and thereby simplyfing the technique of phacoemulsification. The aim of this study is to evaluate the safety of softening lens nucleus by Nd:YAG laser application for cataract operation, and we observed the change of corneal endothleium and IOP and corneal thickness according to varied intensity, and time of laser application. The authors attempted to soften the nucleus using the Q-switched Nd:YAG laser without opening the anterior capsule. The laser was focused into the center of the nucleus and 100, 150 and 200 laser shots were made with the power of 5 mJ, 7 mJ, 10 mJ and 15 mJ. The results were as follows: 1. The corneal thickness was increased to the peak 24 hours after Nd:YAG laser application in cases of 150 and 200 laser shots with 15 mJ (P<0.05). 2. Intraocular pressure was not changed in any group after Nd:YAG laser application. 3. In morphological study with scanning electron microscope, the cases of 200 laser shots with 10 mJ and 150 laser shots with 15 mJ showed moderatly decreased the number of microvilli and partial seperation of the intercellular junctions. The cases of 200 laser shots with 15 mJ showed markedly decreased the number of microvilli and partial seperation of the intercellular junctions.
Astigmatism ; Cataract ; Endothelial Cells* ; Endothelium ; Intercellular Junctions ; Intraocular Pressure* ; Microvilli ; Phacoemulsification ; Refractive Errors

N-buty1-N-(4-hydroxybutyl)nitrosamine(BBN) is known to have a strong. specific carcinogenic action on the urinary bladder of rats. Rat bladder lesions induced by BBN are very similar to those of Pusan histopathologically. To investigate the surface change in the urinary bladder epithelium induced by 0.05% BBN scanning electron microscopic examination was done. Normal bladder epithelium was formed by relatively uniform sized. flat polygonal cells and clearly defined with intercellular ridges. in high magnification, the luminal surface of superficial cell consisted of hexagona1 areas and concave plaques between microridge giving a honeycomb appearance but no microvilli was visible. On BBN administration for 12 weeks, the superficial cells varied in size and shape and covered by numerous uniform round microvilli forming cobble stone appearance. On BBN administration for 12 weeks and water for 6 weeks, the superficial cells were covered with numerous microvilli completely. In some areas numerous pleomorphic microvilli were seen. Although pleomorphic microvilli are not a specific marker for neoplastic transformation, their presence is an indicator. of a markedly abnormal proliferative response.
Animals ; Busan ; Butylhydroxybutylnitrosamine* ; Epithelium* ; Microscopy, Electron, Scanning ; Microvilli ; Phenobarbital ; Rats* ; Urinary Bladder* ; Water

A 27-year-old female with low back pain and paresthesia on the left lower extremity had been treated. Lumbar myelography showed total block of the dye column at the L3 vertebral body level with a brush border. After myelography, total laminectomy on the L3 and L4 vertebrae was done. The result of operation and biopsy revealed an extradural meningioma of angioblastic type. 9 days after the surgery all neurological deficits were completely recovered.
Adult ; Biopsy ; Female ; Humans ; Laminectomy ; Low Back Pain ; Lower Extremity ; Meningioma* ; Microvilli ; Myelography ; Paresthesia ; Spine

To study tubal adhesion, tubal patency and histologic difference under light, electronic microcopy in a site of anastomosis, authors performed three anastomosis surgery in rabbit fallopian tube;1-layer and 2-layer anastomosis in splint-not-used group(group 1) and in splint-used group(group 2) and laser anastomosis in splint-used group(group 3). 10 rabbits were used in each group, therefore the total number of experimental rabbits in 3 groups were 30. The results were as follows; 1. The degree of gross adhesion in group II was significantly high as compared with other groups but there was no difference between left and right tube in the degree of adh- esion in each group(Table 1). 2. The incidence of tubal patency in group I was significantly high(89%) as compared with group I(44%). Especially, the incidence of tubal patency in group III was significantly high(100%) but there was no difference between left and right tube in incidence of tubal patency in each group. 3. Inflammatory cell infiltration appeared in each group on optical microscopic examin- ation. Especially, fibrosis and inflammatory cell infiltration appeared more in group II but there was no statistically significant difference. And there was no significant difference between left and right tube in each group. 4. Transmission electron microscopic examination of group I, as compared with normal control group, showed increase of microvilli and secretory granules and decrease in size of epithelial cell. Epithelial cell was morphologically transformed but maintained the original structure comparatively. TEM examination of group II, as compared with normal control group, showed decrease of microvilli and secretory granules and flattening of epithelial cell. It showed that epithelial cell vanished in it's original structure. TEM examination of group III, as compared with normal control group, showed decrease of microvilli and increase of secretory granules and morphological transformation of epithelial cell. Group III showed the resemblence to normal control group. From the above results, in reversal tuboplasty and tubal factor infertility, it is consi- dered that simple splint-used anastomosis or splint-used anastomosis with Nd-YAG laser will improve the successful rate of tubal anastomosis.
Epithelial Cells ; Fallopian Tubes* ; Female ; Fibrosis ; Incidence ; Infertility ; Lasers, Solid-State* ; Microvilli ; Rabbits ; Secretory Vesicles ; Splints*

We report two cases of ectopic epididymal ducts and efferent ductules in the testicular appendices (TAs) of adult men with normally descended testes. In both cases, a sessile TA was incidentally found at the upper pole of the right testis during the scrotal hydrocelectomy. Microscopically, a few closely arranged tubules were detected within the TA. In the first case, the tubules were lined with a pseudostratified columnar epithelium with numerous, long microvilli, and were surrounded by a smooth muscle coat. In contrast, in the second case, the tubules had a wavy luminal surface, because ciliated columnar cells alternated with groups of cuboidal cells. In both cases, strong CD10 immunoreactivity was observed in the luminal border of the lining epithelium. Surgical pathologists should be aware of the presence of both ectopic epididymal ducts and efferent ductules that can occur in TAs, in order to avoid misinterpretation as transected, functional reproductive structures.
Adult ; Choristoma ; Epididymis ; Epithelium ; Female ; Humans ; Male ; Microvilli ; Muscle, Smooth ; Parovarian Cyst ; Phenobarbital ; Testis ; Wolffian Ducts

The effects of intravesical instillation of mitomycin-C(MMC) as inhibitor of development of experimental bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamine(BBN) were morphologically studied. BBN was administered for 12 weeks orally and then MMC was instilled intravesically once a week with different concentration of 2 mg/ml and 5 mg/ml and doses of 4 and 8 times. There was a significant reduction in incidence if papilloma in group V which received MMC 2 mg/ml for 8 times and also there were significant reductions in incidence of carcinoma in all MMC treated groups except group III which received MMC 2 mg/ml for 4 times. These results indicate that intravesical instillation of MMC is an effective method to prevent bladder carcinogenesis. The ultrastructural effects of MMC were studied by transmission electron microscope. Partial nucleolar fragmentation and decrease in number and height of the surface microvilli of tumor cells resulting in overall increase in intercellular space and cellular detachment from the tumor surface might have played a role in reduction of cancerincidence.
Administration, Intravesical ; Animals ; Carcinogenesis ; Extracellular Space ; Incidence ; Microvilli ; Mitomycin* ; Papilloma ; Rats* ; Urinary Bladder Neoplasms* ; Urinary Bladder*

The effects of intravesical instillation of mitomycin-C(MMC) as inhibitor of development of experimental bladder tumors induced by N-butyl-N-(4-hydroxybutyl) nitrosamine(BBN) were morphologically studied. BBN was administered for 12 weeks orally and then MMC was instilled intravesically once a week with different concentration of 2 mg/ml and 5 mg/ml and doses of 4 and 8 times. There was a significant reduction in incidence if papilloma in group V which received MMC 2 mg/ml for 8 times and also there were significant reductions in incidence of carcinoma in all MMC treated groups except group III which received MMC 2 mg/ml for 4 times. These results indicate that intravesical instillation of MMC is an effective method to prevent bladder carcinogenesis. The ultrastructural effects of MMC were studied by transmission electron microscope. Partial nucleolar fragmentation and decrease in number and height of the surface microvilli of tumor cells resulting in overall increase in intercellular space and cellular detachment from the tumor surface might have played a role in reduction of cancerincidence.
Administration, Intravesical ; Animals ; Carcinogenesis ; Extracellular Space ; Incidence ; Microvilli ; Mitomycin* ; Papilloma ; Rats* ; Urinary Bladder Neoplasms* ; Urinary Bladder*

PURPOSE: To evaluate 20% ethanol toxicity on the rabbit corneal epithelium, ethanol-treated rabbit corneas were examined with electron microscopy. METHODS: Rabbit corneas(24 eyes) were treated with 20% ethanol for 30 seconds, 1 minute, and 2 minutes by using LASEK(Laser Assisted Subepithelial Keratomileusis) instruments, and washed with sterile water. Zero time, 1, 3, 5 days after ethanol treatment, corneas were excised and examined with scanning and transmission electron microscopy. RESULTS: Widespread damage or disappearance of microvilli and local breaks of intercellular junction were observed. The changes were more severe in corneas with longer ethanol treament. In corneas with over 1 minute ethanol treatment, slough of superficial corneal epithelium was shown and increased with time. It was difficult to recognize microvilli or distinctive intercellular junction in corneas with 2 minute-treament. These pathologic changes persisted 5 days after ethanol-treatment. CONCLUSIONS: From these results, 30 seconds to 1 minute-ethanol treatment is recommended in corneal surgery to avoid severe, persisting damage of superficial corneal epithelium.
Cornea ; Epithelial Cells* ; Epithelium, Corneal ; Ethanol ; Intercellular Junctions ; Microscopy, Electron ; Microscopy, Electron, Transmission ; Microvilli ; Water

To determine the effects of contact lens-solution(Uni-cleaner, Uni-soak, Uni-rince on the ocular tissue following instillation for 21 consecutive days, the experimental irritation study was conducted on the rabbit eyes. Nine rabbits(18 eyes) were divided into three groups and each solution was instilled into the eyes four tims a day for 21 consecutive days. Ocular reactions including conjunctival injection, chemosis, sodium fluorescein stain, and erosion were observed and recorded. The reactions were noted using as a guide the scales for scoring from grade I to grade IV according to the severity through the slit lamp examination, and also measured was the corneal thickness change using the ultrasonic pachometer. The results were as follows; 1. No ocular abnormalitis were observed during the slit lamp examination through the test period except one minute after instillation of the solution. 2. The changes of the corneal thickness were not significant statistically(p>0.1). 3. Scanning electronic microscopy showed that epithelial cells formed a polygonal mosaic on surface of the cornea and microvilli and microplicae were present in adjacent cells.
Contact Lens Solutions* ; Cornea ; Epithelial Cells ; Fluorescein ; Microscopy ; Microvilli ; Ultrasonics ; Weights and Measures