BACKGROUND: Although microfat grafting is now used to augment soft tissue, resorption of some amount of fat is inevitable. There are no consistent guidelines for the duration of fat storage. This study evaluated absolute fat mass and pathological changes according to storage duration. METHODS: Nude mice were injected with fresh fat or fat that had been stored for 3 weeks, 5 months, 9 months, 15 months, or 22 months. After 15 weeks, fat graft weight and pathology (viable cells, structural integrity, microvessel formation, cystic degeneration, fibrosis, and cellular infiltration) were assessed. RESULTS: After 15 weeks, the average weight of the remaining fat was 486 mg in the control group and 298, 160, 180, 106, 88, and 80 mg in the 3-week and 5-, 9-, 15-, 22-, and 36-month storage groups, respectively. The average weight of fat tissue significantly decreased to less than 20% in the 5-month group. Also, there was a significant decrease in structural integrity and an increase in cystic degeneration in the 5-month group. Tissue vascularization tended to decrease according to the duration of cryopreservation. CONCLUSIONS: The mean weight of the fat grafts preserved in a general freezer was reduced by 61.3% compared with that of the fresh fat group, which was not statistically significant. The mean fat graft weight was, however, significantly reduced following storage in a general freezer for longer than 5 months. In addition, there were decreases in viable adipocytes and increases in fibrocystic degeneration and inflammatory changes when long-term preserved fat was grafted.
Adipocytes ; Animals ; Cryopreservation* ; Fibrosis ; Mice ; Mice, Nude ; Microvessels ; Pathology ; Transplants*

Humans ; Microvessels ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Plaque, Atherosclerotic ; pathology

Hepatocellular carcinoma (HCC) is a kind of highly aggressive tumor of the digestive system. Several studies have confirmed that microvascular invasion (MVI) is an independent risk factor for early recurrence and poor prognosis of HCC after surgery. Currently, pathological examination is the gold standard for diagnosing MVI. This paper summarizes concept, prognosis, preoperative prediction and treatment plan based on literature review of MVI in HCC.
Carcinoma, Hepatocellular/pathology* ; Humans ; Liver Neoplasms/pathology* ; Microvessels/pathology* ; Neoplasm Invasiveness/pathology* ; Prognosis ; Retrospective Studies

Based on the pathophysiology of the brain, advance in angiogenesis induced by stroke, and evidences of Chinese-medicine-mediated angiogenesis, the possibility to study the stroke-treating mechanism of Chinese medicine in angiogenesis was discussed. And regarding our previous work on angiogenesis modulated by qi-tonifying and stasis-eliminating therapy following intracerebral hemorrhage, we proposed some questions, which should be taken into account in the further work.
Brain ; blood supply ; pathology ; Humans ; Medicine, Chinese Traditional ; Microvessels ; pathology ; Neovascularization, Physiologic ; Stroke ; therapy ; Wound Healing

OBJECTIVETo study the changes in bulbar conjunctiva microcirculation (BCM) and the therapeutic effect of Pentoxifylline on BCM disturbance after high-voltage electrical burn (HEB) in rabbits.

METHODSForty-five rabbits were divided into control group (C), electrical burn group (EB), and Pentoxifylline treatment group (PT) according to random number table, with 15 rabbits in each group. Model of HEB was reproduced in rabbits from EB and PT groups with voltage regulator and experimental transformer. Rabbits in C group were sham injured with the same devices without electrification. Changes in BCM were observed with microcirculation microscope at 15 minutes before HEB and 5 minutes, 1, 2, 4, 8 hour(s) post HEB (PHM or PHH), including: (1) morphology of microvessels, such as the discernible, diameters of arterioles, venules, and capillaries, the unevenness in caliber, and ischemic area; (2) dynamic changes in microvascular blood flow, such as blood flow speed in arterioles, venules, and capillaries, erythrocyte aggregation, and microthrombi formation; (3) condition of tissues surrounding microvessel, such as bleeding and exudation. Measurement data were processed with t test; enumeration data were processed with Fisher's exact test.

RESULTS(1) Morphology of microvessel: discernible of microvessels in EB and PT groups was decreased, but that of PT group was better than that of EB group. At PHM 5, diameter of arterioles, venules and capillaries was respectively (7.3+/-2.5), (12.3+/-2.4), (3.5+/-0.7) microm in EB group, all narrower than those of the control group [(14.6+/-3.1), (27.2+/-3.5), (9.0+/-1.4) microm, with t value respectively 5.23, 13.66, 14.04, P values all below 0.05]. Diameters of the microvessels in PT group [(10.2+/-3.8), (21.5+/-3.1), (7.1+/-1.2) microm] were larger than those in EB group (with t value respectively 2.21, 8.99, 10.18, P values all below 0.05). Diameters of arterioles, venules and capillaries in EB and PT groups recovered to the before HEB size at PHH 1. From PHH 2 to 8, arterioles and capillaries decreased gradually in caliber, venules dilated gradually in EB and PT groups, but the changes in PT group were not obvious. Thickness of microvessel was observed uneven in EB group at PHM 5, which lasted until PHH 8. Ischemia of the tissue was observed in EB group at PHM 5, which improved at PHH 2. Situation in PT group was better. (2) Dynamic changes in microvascular blood flow: at PHM 5, blood flow speed in arterioles, venules and capillaries was respectively (202+/-53), (198+/-44), (46+/-12) microm/s in EB group, all slower than those of the control group [(544+/-37), (359+/-32), (220+/-19) microm/s, with t value respectively 20.47, 11.51, 30.02, P values all below 0.05], and those of PT group [(335+/-42), (260+/-35), (119+/-23) microm/s] were faster than those of EB group (with t value respectively 7.55, 4.26, 14.85, P values all below 0.05). Blood flow speed in EB and PT groups recovered to the before HEB level at PHH 1. From PHH 2 to 8, blood flow speed decreased gradually in EB and PT groups, but that of PT group was faster than that of EB group. Erythrocyte aggregation in venules and capillaries was observed in EB group at PHM 5, which eased up at PHH 1, but aggregated at PHH 2, lasting until PHH 8. Obvious microthrombi were observed in EB group at PHH 2, which increased gradually. These changes were less obvious in PT group. (3) Condition of surrounding tissues of microvessel: in EB group, exudation was observed around microvessels at PHH 1, bleeding at PHH 2, with a worsening tendency. Changes in those in PT group were less obvious.

CONCLUSIONSHEB causes disturbance in BCM, but it can be ameliorated by Pentoxifylline.

Animals ; Burns, Electric ; drug therapy ; pathology ; Conjunctiva ; blood supply ; Microcirculation ; Microvessels ; pathology ; Pentoxifylline ; therapeutic use ; Rabbits

OBJECTIVE: To study the expression of vascular endothelial growth factor(VEGF) and microvessel density (MVD), in nasal polyps and its significance. METHOD: The expression of VEGF, in nasal polyps from 50 patients and inferior turbinates from 10 patients were studied with immunohistochemical methods, measuring their grey score, the relations between expression microvessel density were analyzed. RESULT: (1) The differences between the VEGF expression in nasal polyp gland and vascular were significant (P < 0.05). (2) The differences between the microvessel quantities in nasal polyps and inferior turbinates were significant (P < 0.05). (3) The association between VEGF expression in vascular of nasal polyps and microvessel density was significant (r = -0.664 (< 0. 05). CONCLUSION In nasal polyps, VEGF, expression increased. Those results show that the abnormal expression of VEGF and MVD may play an impotent role in the development of nasal polyps. There was an important value for proper treatment and prediction of nasal polyps.
Humans ; Microvessels ; pathology ; Nasal Polyps ; metabolism ; pathology ; Neovascularization, Pathologic ; Vascular Endothelial Growth Factor A ; metabolism

Through textual research of the literature on tiny collateral and perforator vessel, the two concep- tions were compared and the similarity was analyzed in terms of definition, anatomical level, quantity, the flow of qi and blood and clinical application, etc. It is considered that the tiny collateral in Neijing (Inner Canon of Yellow Emperor) and the perforator vessel of modern medicine are different names of one physical structure. It is proposed that the combination of the tiny collateral theory of CM and the research findings of perforator vessel of western medicine would deepen the understanding of the tiny structure of human skin and promote the development of both TCM and modern medicine.
Books ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians ; Microvessels ; pathology ; Qi

OBJECTIVETo analyze the histologic characteristics of macrochilia secondary to port-wine stain and to elucidate the possible mechanism.

METHODSTwenty-one cases of macrochilia secondary to venular malformation were included and the histology of the lesions was observed by light microscope.

RESULTSHistological examination revealed vascular abnormalities and a number of widely distributed hamartomatous changes in macrochilia secondary to venular malformation. The average vessel diameter is (39.8 +/- 15.7) microm. The degree of hamartomatous change: mild (1 case), moderate (7 cases) and severe (13 cases).

CONCLUSIONSThe complex hamartomatous changes suggest a genetically determined, multilineage developmental field defect in the pathogenesis of venular malformation.

Adolescent ; Adult ; Female ; Hamartoma ; etiology ; pathology ; Humans ; Lip ; abnormalities ; pathology ; Lip Diseases ; etiology ; pathology ; Male ; Microvessels ; pathology ; Middle Aged ; Port-Wine Stain ; complications ; pathology ; Young Adult

OBJECTIVETo explore possible correlations between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Sch-nlein purpura nephritis (HSPN).

METHODSThirty-two children with HSPN were enrolled. They were classified into four groups by renal pathology: HSPN class II (n=8), HSPN class IIIa (n=7), HSPN class IIIb (n=10) and HSPN class IV/V (n=7). Five patients undergoing nephrectomy due to trauma were used as the controls. INFγ, IL-4 and CD34 in the renal tissues were measured by immunohistochemical analysis. INFγ was used as a marker of Th1, IL-4 was used as a marker of Th2 and CD34 was used as a marker of microvessel. The renal microvessel density was evaluated according to the Weidner standard. The relationships among the local Th1/Th2 ratio, renal pathological grade, microvessel score and microvessel density were studied.

RESULTSImmunohistochemical analysis showed a lower expression of INFγ and a higher expression of IL-4 in the HSPN groups than in the control group. The local Th1/Th2 ratio in the HSPN groups decreased and correlated significantly with the renal pathological grade. There were significant differences among four HSPN subgroups (P<0.05). Compared with the control group, the renal microvessel density in the HSPN class II and class IIIa groups increased significantly (P<0.05), but it decreased in the HSPN class IV/V group (P<0.05). The renal microvessel scores in the HSPN class IIIa, class IIIb and class IV/V groups increased significantly compared with those in the control and the HSPN classⅡ. The increased renal microvessel scores were associated with more severe renal pathological changes. A negative correlation was found between the local Th1/Th2 ratio and the microvessel density in kidneys (r=-0.921, P<0.01).

CONCLUSIONSThe decrease of Th1/Th2 ratio in kidneys might be responsible for renal microvascular injury in children with HSPN.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Kidney ; blood supply ; pathology ; Male ; Microvessels ; pathology ; Nephritis ; immunology ; pathology ; Purpura, Schoenlein-Henoch ; immunology ; pathology ; Th1 Cells ; immunology ; Th2 Cells ; immunology

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Microvascular invasion (MVI) is considered the major risk factor for postoperative recurrence and metastasis in HCC. The diagnosis of MVI relies on the postoperative pathological assessment of the tumor tissues. Seeking non-invasive methods and biomarkers for evaluation of MVI before surgery has important clinical implications for guiding surgical treatment and improving patients' survival. Recent studies have reported the applications of radiomics technique in prediction of MVI in HCC and showed promising results. Herein we summarized the research progress in CT- or MRI-based radiomics models for prediction of MVI in HCC to provide helpful thinking for further research in this field.
Biomarkers ; Carcinoma, Hepatocellular/pathology* ; Humans ; Liver Neoplasms/pathology* ; Magnetic Resonance Imaging ; Microvessels/pathology* ; Neoplasm Invasiveness/pathology* ; Retrospective Studies ; Tomography, X-Ray Computed/methods*