No abstract available.
Humans* ; Knee* ; Ligaments* ; Microvessels*

Using Mercox CL-2B, intravascular casting was performed follwoing intracameral injection with 2.0microgram/microliter of Endothelin-1(ET-1) in the rabbit eye. One hour after intracameral injection with ET-1 caused severe focal andsegmental constriction of major arterial circle(MAC). Obstruction of the small branch of MAC caused localized filling defect and decreased number of capillaries in the vascular cast. The diameters of vascular cast were reduced at about 22.2% in proximal MAC, 22.4% in distal MAC and 21.8% in first branch of MAC. This results were suggested that ET-1 may play a role in the regulation of blood flow in the anterior segment vasculature of the rabbit.
Capillaries ; Constriction ; Endothelin-1* ; Microvessels*

BACKGROUND: In order to evaluate the clinical relevance of angiogenesis in patients with gastric cancer, we investigated the microvessel count in gastric cancer tissues and compared the results with several clinicopathologic factors and the prognosis for the same stage. METHODS: A total of 256 patients with gastric cancer were included in this study. The microvessel count was determined by using immunohistochemical staining monoclonal antibody against factor- VIII-related antigen. RESULTS: The microvessel density determined by using immunohistochemical staining for factor-VIII- related antigen had statistical significance with survival between the higher (> or =42: microvessel counts) and lower the (<42: microvessel counts) groups. However in the same stage, except stage I, it had not significant correlation with survival. CONCLUSION: The tumor angiogenesis of a gastric carcinoma in stage I is an independent factor that impacts on the prognosis.
Humans ; Microvessels ; Prognosis ; Stomach Neoplasms

Humans ; Microvessels ; Neovascularization, Pathologic ; Neovascularization, Physiologic ; Plaque, Atherosclerotic ; pathology

No abstract available.
Brain Edema* ; Brain* ; Microvessels* ; Vascular Endothelial Growth Factor A*

Tumor angiogenesis has been found to have prognostic significance in many tumor types for predicting an increased risk of metastasis. We assessed tumor vascularity in 28 cases of borderline malignancy and 71 cases of carcinoma of the ovary which had been resected and diagnosed, using the highly specific endothelial cell marker CD34. The numbers of microvessels were counted in 200 magnification in three highly vascularised areas. The numbers of microvessels in carcinomas were higher than that in the borderline malignancy of serous and mucinous tumors. The number of microvessels of mucinous carcinomas was significantly higher than that of serous carcinomas. There were neither significant differences in the number of microvessels according to histological tumor types (p=0.075) nor significant differences in the number of microvessels according to FIGO stages (p=0.072). But in serous carcinomas, the number of microvessels was higher in the FIGO III-IV stage than in the FIGO I-II stage (p=0.017). This study showed higher neovascularization in malignant tumor than borderline malignancy, and in the advanced stage (FIGO III-IV) than less advanced stage (FIGO I-II) of serous carcinomas.
Adenocarcinoma, Mucinous ; Endothelial Cells ; Female ; Microvessels ; Mucins ; Neoplasm Metastasis ; Ovary

PURPOSE: This study was designed to examine the microvessel count and p53 protein expression level in pancreatic adenocarcinoma patients and to determine how they correlate with the clinicopathologic factors and prognosis. In addition, the relationship between angiogenesis and p53 protein expression in pancreatic adenocarcinoma patients was investigated. METHODS: Paraffin-embedded tumor tissues from 30 patients with pancreatic adenocarcinomas that were completely removed by a radical resection were retrieved and analyzed. The vessels were immunostained with anti-factor VIII polyclonal antibodies, and 5 areas with the most discrete microvessels were counted under a 200 field. The mean was calculated, which was defined as the angiogenesis score (AS). p53 protein expression was detected by the immunohistochemical stain method. RESULTS: The mean AS was 37.7 13.6. There were no significant differences in sex, age, tumor size, histologic grade, T category (depth of invasion), N category (lymph node metastasis), pathologic stage and recurrence between the high and low AS group. The p53 protein expression rate was 73.3%, which did not correlate with the clinicopathologic factors and AS. However, recurrence was significantly related to the histologic grade and pathologic stage (p=0.040 and p=0.029). Among the various clinicopathologic factors, the N category (lymph node metastasis), the AS and recurrence (p=0.025, p=0.029 and p=0.003) were statistically significant according to a univariate survival analysis. In theCox regression analysis, the N category (lymph node metastasis) and recurrence were independent prognostic factors (odds ratio=4.344, 4.263). CONCLUSION: Although the survival rate in the high AS group was significantly lower than that in low AS group (p=0.029), the angiogenesis assessed by the microvessel count using immunohistochemical staining was not an independent prognostic factor in the Cox regression analysis.
Adenocarcinoma* ; Antibodies ; Humans ; Microvessels ; Prognosis ; Recurrence ; Survival Rate

Newly introduced direct peroral cholangioscopy and the development of video choledochoscopes have enabled more defined observation of bile duct mucosal lesions with clearer images. Narrow-band imaging (NBI) is a unique endoscopic imaging technology that provides enhanced endoscopic images of surface mucosal structures and its superficial microvessels. Advanced cholangioscopy and NBI are expected to be useful for precise evaluation and correct diagnosis of biliary tract diseases. However, the diagnostic value of advanced bile duct imaging with cholangioscopy requires further evaluation.
Bile Ducts* ; Bile* ; Biliary Tract Diseases ; Biliary Tract* ; Diagnosis ; Microvessels

BACKGROUND: Angiogenesis is one of the most important factors in the progression and me-tastasis of malignancies. Angiogenesis is a multistep process requiring the interaction of numerous factors able to stimulate the growth and development of new vessels. But, understanding of the mechanism involved in VEGF expression is unclear. METHODS: Expressions of p53 and VEGF, and neovasculiarization were examined in 19 cases of surgically resected non-small cell carcinoma of the lung by the immunohistochemical staining. Furthermore, VEGF mRNA expressions were quantified in all cases using the real-time quantitative RT-PCR. These results were compared with clinicopathologic parameters such as histologic grade and stage. RESULTS: Tumors with high aberrant p53 expressions showed significantly higher VEGF mRNA ex-pressions and microvessel counts than those with low p53 expressions. Expressions of p53 as well as VEGF and micovessel counts were closely associated with the tumor stage, but not with the histologic grade and other clinical parameters. CONCLUSIONS: These results suggest that aberrant p53 expression may play a role in the regulation of VEGF expression and may be involved in controlling angiogenesis in non-small cell carcinoma of the lung.
Growth and Development ; Lung* ; Microvessels ; RNA, Messenger* ; Vascular Endothelial Growth Factor A*

PURPOSE: The prognostic factors for superficial bladder tumor that affect tumor recurrence and progression have been studied for many years. They are stage, grade, size, multiplicity, microvessel invasion and etc. This study was performed to evaluate the influence of the tumor configuration on predicting its progression and recurrence. MATERIALS AND METHODS: 128 patients who initially presented with superficial bladder tumor (pTis, pTa and pT1) were retrospectively analyzed according to many factors such as stage, grade, size, multiplicity, microvessel invasion and tumor configuration for tumor progression and recurrence after primary transurethral resection. RESULTS: 48 patients (37.5%) experienced recurrent disease and 20 patients (15.6%) had progressive disease. The absence of stalk and microvessel invasion were the statistically significant factors for recurrence. Only microvessel invasion was a significant prognostic factor for progression. CONCLUSIONS: Only two factors, i.e., the absence of tumor stalk and microvessel invasion, were significant prognostic factors for tumor recurrence. Only microvessel invasion was a significant prognostic factor for tumor progression. When tumors are microvessel invasion positive without stalk after primary transurethral resection, these patients might then benefit from being treated with a more aggressive therapeutic modality.
Humans ; Microvessels ; Recurrence ; Retrospective Studies ; Urinary Bladder Neoplasms* ; Urinary Bladder*