PURPOSE: The aim of this study was to evaluate the effect of Er:YAG laser on microstructure and roughness of TiO2 blasting implant surface. MATERIALS AND METHODS: Ten TiO2 blasting implant were used in this experiment. One implant was control group, and nine TiO2 blasting implant surfaces were irradiated with Er:YAG laser under 100 mJ/pulse, 140 mJ/pulse, and 180 mJ/pulse condition for 1 min, 1.5 min, and 2 min respectively. Optical interferometer and scanning electron microscopy was utilized to measure roughness and microstructure of specimens. RESULTS: The surface roughness was decreased after Er:YAG laser irradiation in all groups, but there was no significant difference. 100 mJ/pulse and 140 mJ/pulse group did not alter the TiO2 blasting implant surface in SEM study while 180 mJ/pulse group altered the TiO2 blasting implant surface. Implant surfaces showed melting, microfracture and smooth surface in 180 mJ/pulse group. CONCLUSION: Detoxification of implant surface using Er:YAG laser must be irradiated with proper energy output and irradiation time to prevent implant surface alteration.
Freezing ; Microscopy, Electron, Scanning

Megaselia scalaris (Loew) (Diptera: Phoridae) is a cosmopolitan scuttle fly of medical and forensic importance. This species is generally small, humpbacked and is a prominent decomposer of corpses indoors. Taxonomically, adult sexes can be distinguished based on the characteristics of the terminal segments of the abdomen. In this report, the terminalia of adult male and female M. scalaris were examined using scanning electron microscope (SEM). The terminal segment of an adult female is less complex compared to male, consisting of an ovipositor and cerci. In male, the hypopygium consists of epandrium, hypandrium, anal tube and penis complex. A pair of long and feathered setae was attached to the tip of the anal tube and tapered. The application of SEM to identify this species isuseful and can be expanded to other species in this fly group.
Microscopy, Electron, Scanning

OBJECTIVES: The aim of this study was to evaluate the mechanical and physical properties of a newly developed fiber reinforced dental composite. MATERIALS AND METHODS: Fiber reinforced composite EverX Posterior (EXP, GC EUROPE), and other commercially available bulk fill composites, including Filtek Bulk Fill (FB, 3M ESPE), SonicFill (SF, Kerr Corp.), SureFil (SDR, Dentsply), Venus Bulk Fill (VB, HerausKultzer), Tetric evoceram bulk fill (TECB, Ivoclar Vivadent), and Xtra Base (XB, Voco) were characterized. Composite samples light-cured with a LED device were evaluated in terms of flexural strength, flexural modulus (ISO 4049, n = 6), fracture toughness (n = 6), and Vickers hardness (0, 2, and 4 mm in depth at 24 hr, n = 5). The EXP samples and the fracture surface were observed under a scanning electron microscopy. Data were statistically analyzed using one-way ANOVA and unpaired t-test. RESULTS: EXP, FB, and VB had significantly higher fracture toughness value compared to all the other bulk composite types. SF, EXP, and XB were not statistically different, and had significantly higher flexural strength values compared to other tested composite materials. EXP had the highest flexural modulus, VB had the lowest values. Vickers hardness values revealed SF, EXP, TECB, and XB were not statistically different, and had significantly higher values compared to other tested composite materials. SEM observations show well dispersed fibers working as a reinforcing phase. CONCLUSIONS: The addition of fibers to methacrylate-based matrix results in composites with either comparable or superior mechanical properties compared to the other bulk fill materials tested.
Hardness ; Microscopy, Electron, Scanning ; Venus

The purpose of this study was to compare the changes of retentive force in different stud attachment systems for implant retained overdenture. Two commercially available attachments with different retentive forces were investigated: Kerator (pink, blue, red) and O-ring (orange, red). Two implant fixtures were vertically embedded in base mountings. Five pairs of each attachment were evaluated. A universal testing machine was used to evaluate the retentive force of two attachments during wear simulation. Surface characteristics of each attachment system were evaluated with scanning electron microscopy. Five pairs of each attachment were evaluated. Kerator pink showed the highest initial retention. After 2,500 cycles of wear-simulation, Kerator pink noted the largest decrease in retention. According to results of surface analysis, Worn surfaces were obtained in matrices and patrices. Heavy wears were observed in matrices. After 2 year-wear simulation, most attachments exhibited retention loss. Attachments using different kind of material exhibited dissimilar surface alterations.
Denture, Overlay* ; Microscopy, Electron, Scanning

BACKGROUND AND OBJECTIVES: Colonization of microorganisms on pollutants is the first indication of the potential ability of microbes to utilize plastic pollutants as a carbon source by sequential biodegradation into usable form for sustenance. The Philippines is considered the third highest country contributing to global mismanaged plastic waste. To locally manage and find a natural and innovative solution to this worldwide concern, this study aimed to evaluate the capacity of Xylaria sp. SDM (sterile dark mycelia) wild type, which was previously reported to colonize polyethylene plastic and mutant strains to colonize polystyrene, a plastic pollutant widely produced in the world. Assessment of the ability of local Xylaria sp. strains to grow, penetrate, and damage the surface and inner structures of polystyrene was investigated using scanning electron microscopy (SEM).

METHODOLOGY: Xylaria sp. strains were cultured in a pH 5.0 mineral medium with 0.5% glucose as carbon source and polystyrene as a co-carbon source, and stored at 25?C for 50 days. At the end of the incubation period, due to irremovable fungal strains on the surface of the polystyrene strips, samples of polystyrene from each strain were subjected to SEM.

RESULTS: On the 20th day of incubation, the presence of mucilaginous sheaths and fungal growth was observed on the surface of treated polystyrene strips. At the end of the 50-day incubation period, scanning electron microscopy (SEM) confirmed fungal growth and colonization, through the presence of mycelial mats and hyphae, of the wild type and mutant strains on the surface and inner structures of polystyrene except the control. Moreover, physical surface damage in the form of holes, cracks, and crevices on polystyrene demonstrated the active burrowing action of Xylaria sp. strains further supporting the potential of this fungus to damage polystyrene plastic.

CONCLUSION: Whereas fungal growth on a polymer surface is necessary but not sufficient to conclude the process of carbon assimilation as the final biodegradation step, the initial colonization of Xylaria sp. strains on polystyrene supports its ability to establish itself and physically damage the pollutant. Hence, this study extended the existing knowledge on the colonizing ability of Xylaria sp. on plastic making it a potential candidate organism to biodegrade plastic waste, which is one of the topmost environmental waste hazards in the world today.

The section of Annulata under genus Hypoxylon is a member of the Xylariaceae which has ascomata with ostioles papillate ill a flattened disc. The isolates of Hypoxylon collected from the Tropic to the Temperate are compared with similar taxa and described. These isolations under Annulata section are illustrated with a light microscopy and a scanning electron microscopy. Hypoxylon archeri is newly recorded from Korea. A key to the species is also included.
Fungi* ; Korea ; Microscopy ; Microscopy, Electron, Scanning ; Wood*

Melanosomes were isolated from the human hair by graded centrifugation and identified by transmission and scanning electron microscopic examination. Melanosomes were separated from the keratinous structures by treating with strong NaOH solution for 15 hours. The keratinous structures were removed by centrifugation ai 2,500xg and 3,500xg for 10 minutes respectively at 0 ℃. The isolated melanosomes were collected by centrifugation at 7,800xg at 0 ℃. Scanning electron microscopic examination made it possible to evaluate the global structure of purified melanosomes.
Centrifugation ; Hair* ; Humans* ; Melanosomes* ; Microscopy, Electron, Scanning

In July 2012, philometrid nematodes were discovered in cultured rockfish (Sebastes schlegeli) in Cheonsuman (Bay), the Republic of Korea. The nematodes were detected in the epithelial tissues of the rockfish and were identified as Clavinema mariae based on morphological studies using light and scanning electron microscopy. They revealed the characteristics same as previously identified C. mariae, notably having a long body with narrow posterior half, no caudal projection, a cylindrical-shaped esophagus, a well-developed anterior bulbous part of the esophagus, cephalic papillae, and a dorsal esophageal gland. This is the first confirmation of C. mariae infection in rockfish in Korea.
Esophagus ; Korea ; Microscopy, Electron, Scanning ; Republic of Korea*

OBJECTIVE: The purpose of this study was to evaluate the surface roughness (SR) of 2 types of orthodontic archwires made by 4 different manufacturers. METHODS: This in vitro experimental study was conducted on 35 specimens of 7 different orthodontic archwires, namely, 1 nickel-titanium (NiTi) archwire each from the manufacturers American Orthodontics, OrthoTechnology, All-Star Orthodontics, and Smart Technology, and 1 stainless steel (SS) archwire each from the manufacturers American Orthodontics, OrthoTechnology, and All-Star Orthodontics. After analyzing the composition of each wire by energy-dispersive X-ray analysis, the SR of each wire was determined by scanning electron microscopy (SEM) and surface profilometry. Data were analyzed using the Kruskal-Wallis and Mann-Whitney U tests (alpha < 0.05). RESULTS: The average SR of NiTi wires manufactured by Smart Technology, American Orthodontics, OrthoTechnology, and All-Star Orthodontics were 1,289 +/- 915 Adegrees, 1,378 +/- 372 Adegrees, 2,444 +/- 369 Adegrees, and 5,242 +/- 2,832 Adegrees, respectively. The average SR of SS wires manufactured by All-Star Orthodontics, OrthoTechnology, and American Orthodontics were 710 +/- 210 Adegrees, 1,831 +/- 1,156 Adegrees, and 4,018 +/- 2,214 Adegrees, respectively. Similar to the results of profilometry, the SEM images showed more defects and cracks on the SS wire made by American Orthodontics and the NiTi wire made by All-Star Orthodontics than others. CONCLUSIONS: The NiTi wire manufactured by All-Star Orthodontics and the SS wire made by American Orthodontics were the roughest wires.
Microscopy, Electron, Scanning ; Orthodontics ; Stainless Steel

A scanning electron microscopic study was performed on the surface ultrastructure of Pygidiopsis summa (Digenea: Heterophyidae) adults. Metacercariae were collected from gills and muscles of mullets (Mugil cephalus) caught in a known endemic area, and adult flukes were harvested from dogs after 8 weeks of experimental infection. The worm was calabash form with its posterior part broader than the anterior part. Tegumental spines were densely distributed over the body surface, except on the suckers and genital apparatus, and around the excretory pore. Well differentiated spines were observed on the anterior half of the body, with 14-16 tips ventrally, and 19-20 tips dorsally. On the oral sucker, three pairs of type I sensory papillae (uni-ciliated knob-like swellings) and one pair of type II sensory papillae (aciliated round-swellings) were observed on the anterior and posterior parts of the lip, respectively. On the lip of the ventral sucker, one pair of type II sensory papillae was distributed only on its posterior part. Sperms were seen emerging from or entering into the genital apparatus. The results showed that the surface ultrastructure of P. summa was unique among the heterophyid trematodes, especially in digitation of tegumental spines and in distribution of sensory papillae on oral and ventral suckers.
Animals ; Dogs ; Heterophyidae/*ultrastructure ; Microscopy, Electron, Scanning