No abstract available.
Microdissection*

No abstract available.
Microdissection*

No abstract available.
Clone Cells* ; Microdissection*

Several different techniques have been utilized for the correction of a varicocele. Urologists have operated by high ligation of the internal spermatic veins via an inguinal or retroperitoneal approach. Radiologists have embolized or sclerosed these veins by percutaneous venography. Recently, microsurgeons have diverted the internal spermatic veins. Although classical procedures for the varicocele have been used successfully to eliminate retrograde blood flow, improved techniques seen desirable because a growing list of unwanted effects have been cited in associations with these procedures. In this report, we describe a new technique(Goldstein microsurgical technique) for the management of varicoceles which combines microdissection of the cord, ligation of all the tributaries of internal spermatic vein. complete control of collateral and small cross collateral veins except the vasal vein via a low inguinal approach.
Ligation ; Microdissection* ; Phlebography ; Spermatic Cord* ; Varicocele* ; Veins

The cystic disease of the kidney include a heterogeneous group of developmental, hereditary, and acquired disorders. Based on extensive microdissection studies, Potter concluded all renal cystic diseases could be categorized into four types. We have experienced 5 cases of cystic kidney disease which were confirmed by aoutopsy and classified as Type I, Type II, Type III, Boderline between types II and III and Type IV according to Potter's classification. We report these cases with a review of literatures.
Classification* ; Kidney ; Kidney Diseases, Cystic* ; Microdissection

OBJECTIVE: We have introduced a method of characterization of invading glioma cells by using molecular analysis of marginal invading tumor cells and molecular profiles of glioma tumor margin. METHODS: Each of tumor core and marginal tissues was obtained in 22 glioma patients. Tumor core cells and marginal cells from each glial tumor were collected by laser capture microdissection or intraoperative microdissection under the operating microscope. Expression of MMP-2, MMP-9, CD44 and RHAMM mRNA by invading glioma cells compared with tumor core was confirmed by realtime-PCR of twenty-four glioma specimens. Clinical data also were reviewed for invasion and recurrence pattern of the gliomas radiologically and invasive rim pattern microscopically. RESULTS: Overall results of the molecular analysis showed that relative overexpression of MMP-2, MMP-9 and RHAMM were noted at the invasive edge of human glioma specimens comparing to the tumor core but CD44 was highly expressed in the tumor core comparing to the margin. High marginal expression of MMP-2 and MMP-9 were noted in poorly ill-defined margin on the pathological finding. High marginal expression of CD44 and MMP-2 were demonstrated in the midline cross group on the radiological review, and that of RHAMM and MMP-2 were showed in the aggressive recurrence group. High expression of MMP-2 seems to be involved in the various invasion-related phenomenons. CONCLUSION: Up-regulation of MMP-2, MMP-9, CD44 and RHAMM was noted in invasive edge of gliomas according to the various clinical situations.
Glioma* ; Humans ; Laser Capture Microdissection ; Microdissection ; Recurrence ; RNA, Messenger ; Up-Regulation

The detailed knowledge of the microvascular anatomy and the recognition of the anatomical variations of the middle cerebral artery (MCA) are very important for neurosurgeons to construct a better and safer microdissection plan and to prevent postoperative neurological deficits. This paper mainly reviewed segments, perforators, cortical arteries, branching patterns, anomalies, and regional anatomy relating various neurovascular surgery such as vascular anastomosis and the management of aneurysms and arteriovenous malformations of the MCA.
Anatomy, Regional ; Aneurysm ; Arteries ; Arteriovenous Malformations ; Microdissection ; Middle Cerebral Artery*

The detailed knowledge of the microvascular anatomy and the recognition of the anatomical variations of the middle cerebral artery (MCA) are very important for neurosurgeons to construct a better and safer microdissection plan and to prevent postoperative neurological deficits. This paper mainly reviewed segments, perforators, cortical arteries, branching patterns, anomalies, and regional anatomy relating various neurovascular surgery such as vascular anastomosis and the management of aneurysms and arteriovenous malformations of the MCA.
Anatomy, Regional ; Aneurysm ; Arteries ; Arteriovenous Malformations ; Microdissection ; Middle Cerebral Artery*

When traditional flap techniques are not feasible, we apply flap prefabrication, which is more complicated and sophisticated but supplies large and thin flaps. There are some disadvantages to the technique that require improvement, such as venous congestion after flap transfer, which requires months for neoangiogenesis and necessitates a vascular carrier. Here, the author presents a new technique, called as ‘microdissected prefabricated flap,’ to successfully produce a safe, large, and thin flap. This technique is based on the microdissection of the perforators to the greatest extent possible, spreading them out into the subdermal level and using them as a carrier. The details and the application of this technique are presented and reported.
Equipment and Supplies ; Fluorescein ; Hyperemia ; Microdissection ; Microsurgery ; Surgical Flaps

The cultured cells were derived from rabbit corneal stroma by explant technique following microdissection and serial passage. The ultrastructural features of fourteenth-passage keratocytes were examined with both scanning and transmission electron microscope. The cells can be divided into activated, intermediate and old cell according to the differences in electron density and surface microvillous pattern. The morphologic characteristics of the cultured keratocytes partially resemble those shown in corneal keratocytes in vivo.
Cells, Cultured ; Corneal Keratocytes* ; Corneal Stroma ; Microdissection ; Serial Passage