1.Progress of study on the detection technique of microRNA.
Journal of Experimental Hematology 2009;17(6):1602-1604
MicroRNAs (miRNAs) are small noncoding RNA molecules that negatively regulate gene expression via degradation or translational repression of their targeted mRNAs. MiRNAs are involved in critical biologic processes, including development, cell differentiation, proliferation and the pathogenesis of disease. This review focuses on recent researches on the detection techniques of miRNA including micorarray technique, Northern blot, real-time quantitative PCR, detection technique of miRNA function and so on.
Blotting, Northern
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MicroRNAs
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genetics
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isolation & purification
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Microchip Analytical Procedures
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Oligonucleotide Array Sequence Analysis
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Polymerase Chain Reaction
2.Application of biochip technology and its application on medical diagnosis.
Chinese Journal of Medical Instrumentation 2013;37(5):355-357
Biochip analytical technology shows high throughput property for multi-samples measurement, so can reduce the required amount of samples and time used for determination. The technology quickly developed in recent years and has been applied in medical diagnosis and other analytical areas including gene chip, protein chip, lab-on-a-chip, tissue microarray, cell microarray, carbohydrate microarray and so on. This paper overviewed the current development of biochip technology, and explored the perspective of its application.
Diagnostic Techniques and Procedures
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Microchip Analytical Procedures
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methods
5.The advance of research in electro-physiology and chip techniques for olfaction and gustation.
Ying XU ; Yan LI ; Gaixia XU ; Lifeng QIN ; Rong LI ; Xuesong YE ; Ping WANG
Journal of Biomedical Engineering 2006;23(1):216-219
This paper introduces the new research achievement and progress of electro-physiology in olfaction and gustation. Classical implements such as patch-clamp or glass pipette are not appropriate in the dynamic detection of cellular signal transportation. In view of this, we have analyzed the feasibilities and challenges of olfactory or gustatory cell-based biosensors such as field effect transistor (FET) and light addressable potentiometric sensor (LAPS). Finally we present the research work carried our in out lab and a future prospective on the development in this field.
Biosensing Techniques
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instrumentation
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methods
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Electrophysiology
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methods
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Humans
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Microchip Analytical Procedures
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methods
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Smell
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physiology
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Taste
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physiology
6.Biochip micromachining technology adopting laser technology.
Yu ZHANG ; Shengqi WANG ; Tiechuan ZUO ; Tao CHEN
Journal of Biomedical Engineering 2005;22(1):160-162
This paper deals with the manufacturing state of the art of biochip, and introduces a new method--laser microtechnology, including its developing procedure, characteristics and function in biochip production.
Biosensing Techniques
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instrumentation
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methods
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Computer-Aided Design
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Equipment Design
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Lab-On-A-Chip Devices
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Lasers
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Microchip Analytical Procedures
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methods
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Micromanipulation
7.Development of a print quality inspection system for biochips.
Ai-Ke QIAO ; Xian-Long MENG ; Zhang-Jun MA ; Hong-Bin ZHANG ; Bo CHU
Chinese Journal of Medical Instrumentation 2008;32(6):434-437
An automatic inspection system for biochip's print quality is presented in this paper. It consists of an automatic mechanical control, a CCD sensor for getting the image of PET boart, and the special computer software for image processing and recognition. Experimental results indicate that this system is capable of providing a precise and effective realtime inspection for biochips' print quality.
Biosensing Techniques
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instrumentation
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methods
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Equipment Design
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Image Processing, Computer-Assisted
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methods
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Microchip Analytical Procedures
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methods
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Pattern Recognition, Automated
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methods
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Quality Control
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Software
8.Applicability of the multiplex quantitative antibody array system for early diagnosis of hepatocellular carcinoma.
Dong-mei GAO ; Lu SUN ; Kun GUO ; Yan LI ; Yin-kun LIU ; Xiao-nan KANG
Chinese Journal of Hepatology 2012;20(10):785-788
OBJECTIVETo develop an early and accurate detection method for hepatocellular carcinoma (HCC) based on detection of tumor-associated serum markers using a multiplex quantitative antibody array.
METHODSThe double-antibody sandwich principle was used to establish an antibody array composed of eight cancer-related serum markers, including alpha-fetoprotein (AFP), hepatocyte growth factor (HGF), insulin-like growth factor (IGF), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-b1), and vascular endothelial growth factor (VEGF). Serum samples from 160 cases of clinically diagnosed HCC and from 58 cases of liver cirrhosis (LC; controls) were obtained to test the array. Sixty percent of the samples were randomly selected for use as the training set (HCC, n = 96; LC, n = 36), and the remaining 40% was used as the test set (HCC, n = 64; LC, n = 22). The SPSS statistical software was used to perform logistic regression analysis and to create a diagnostic model.
RESULTSWhen used with the training set, the model had sensitivity of 93.3%, specificity of 83.3%, and accuracy of 90.9%. When used with the test set, the model had sensitivity of 89.0%, specificity of 77.3%, and accuracy of 86.0%. The traditional serum AFP value (cut-off value of 20 ng/mL) showed 70.0% diagnostic sensitivity, 59.0% specificity, and 64.0% accuracy.
CONCLUSIONThe newly developed multiplex quantitative antibody detection system has high sensitivity and specificity. The diagnostic model with AFP and seven other cancer-related factors was superior to the traditional AFP only approach for early diagnosis of liver cancer, indicating its potential clinical value.
Adult ; Aged ; Biomarkers, Tumor ; blood ; Carcinoma, Hepatocellular ; diagnosis ; Early Diagnosis ; Female ; Humans ; Liver Neoplasms ; diagnosis ; Male ; Microchip Analytical Procedures ; Middle Aged ; Sensitivity and Specificity ; Young Adult ; alpha-Fetoproteins ; immunology
9.BRG1 expression in prostate carcinoma by application of tissue microarray.
Yan LI ; Qun-li SHI ; Xing-zao JIN ; Kui MENG ; Xiao-jun ZHOU ; Li-ping SUN
National Journal of Andrology 2006;12(7):629-632
OBJECTIVETo investigate the expression of gene BRG1 in prostatic intraepithelial neoplasia and adenocarcinoma, and the relationship between gene BRG1 expression and the clinicopathological features of prostate carcinoma.
METHODSGene BRG1 expression was evaluated in 37 cases of human prostate carcinoma, 13 human prostatic intraepithelial neoplasia (PIN) and 14 human benign prostatic hyperplasia (BPH) by using immunohistochemistry (EnVision method) and tissue microarray.
RESULTSThe positive rates of BRG1 protein were 81.08% (30/37), 38.46% (5/13) and 14.28% (2/14) in prostate carcinoma, PIN and BPH, respectively, significantly higher in the first group than in the latter two (P < 0.05). There was no statistically significant difference in BRG1 gene expression either between PIN and BPH (P > 0.05) or between the groups of the moderate differentiation (the Gleason histologic grading: 5-7) and the lower one (the Gleason histologic grading: 8-10) (P > 0.05).
CONCLUSIONBRG1 may play an important role in the development of prostate carcinoma. Tissue microarray technology, with the advantages of high throughput, conciseness, rapidity, high efficiency, low cost, and nice reproducibility, has significant practical value and broad application prospects in pathology.
Aged ; Aged, 80 and over ; DNA Helicases ; biosynthesis ; Humans ; Immunohistochemistry ; Male ; Microchip Analytical Procedures ; Middle Aged ; Nuclear Proteins ; biosynthesis ; Prostatic Neoplasms ; metabolism ; pathology ; Reproducibility of Results ; Transcription Factors ; biosynthesis
10.Functional Profiling of Human MeCP2 by Automated Data Comparison Analysis and Computerized Expression Pathway Modeling.
Injoo KIM ; Shin Hae LEE ; Jinwoo JEONG ; Jun Hyung PARK ; Mi Ae YOO ; Cheol Min KIM
Healthcare Informatics Research 2016;22(2):120-128
OBJECTIVES: Methyl-CpG binding protein 2 (MeCP2) is a ubiquitous epigenetic factor that represses gene expression by modifying chromatin. Mutations in the MeCP2 gene cause Rett syndrome, a progressive neurodevelopmental disorder. Recent studies also have shown that MeCP2 plays a role in carcinogenesis. Specifically, functional ablation of MeCP2 suppresses cell growth and leads to the proliferation of cancer cells. However, MeCP2's function in adult tissues remains poorly understood. We utilized a weight matrix-based comparison software to identify transcription factor binding site (TFBS) of MeCP2-regulated genes, which were recognized by cDNA microarray analysis. METHODS: MeCP2 expression was silenced using annealed siRNA in HEK293 cells, and then a cDNA microarray analysis was performed. Functional analysis was carried out, and transcriptional levels in target genes regulated by MeCP2 were investigated. TFBS analysis was done within genes selected by the cDNA microarray analysis, using a weight matrix-based program and the TRANSFAC 6.0 database. RESULTS: Among the differentially expressed genes with a change in expression greater than two-fold, 189 genes were up-regulated and 91 genes were down-regulated. Genes related to apoptosis and cell proliferation (JUN, FOSL2, CYR61, SKIL, ATF3, BMABI, BMPR2, RERE, and FALZ) were highly up-regulated. Genes with anti-apoptotic and anti-proliferative functions (HNRPA0, HIS1, and FOXC1) were down-regulated. Using TFBS analysis within putative promoters of novel candidate target genes of MeCP2, disease-related transcription factors were identified. CONCLUSIONS: The present results provide insights into the new target genes regulated by MeCP2 under epigenetic control. This information will be valuable for further studies aimed at clarifying the pathogenesis of Rett syndrome and neoplastic diseases.
Adult
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Apoptosis
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Binding Sites
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Carcinogenesis
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Carrier Proteins
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Cell Proliferation
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Chromatin
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Epigenomics
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Gene Expression
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HEK293 Cells
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Humans*
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Methyl-CpG-Binding Protein 2
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Microarray Analysis
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Oligonucleotide Array Sequence Analysis
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Rett Syndrome
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RNA, Small Interfering
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Transcription Factors