Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental pollutants with high toxicity. It is a potent way to remove these pollutions by microbe degradation and conversion, and many bacteria have been found performing this function. Here the bacterial catabolic enzymes and genes participating in upper pathway of PAHs degradation were revealed, the genetic properties were illuminated, and the gene evolution of PAHs catabolism was further discussed. It would contribute to elucidating PAHs degrading mechanisms in bacteria and provide theoretical evidence for achieving efficient bioremediation.

A strain,WJ-100, with high flocculating activity was screened from activated sludge.Its optimum pH was 6.5 and optimum temperature was 25℃～40℃.Its shaking speed was in the range of 80～240 r/min. Na~ + ,K~ + ,Ca~ 2 + and Fe~ 3+ could accelerate flocculation .Ca~ 2+ did the most obviously.WJ-100 can use various saccharides as good sources of carbon,the flocculating rate reached 99.2%,99.8%,even 100%.The strain could flocculate very well when pH of Kaolin clay suspension was 2.0～10.0.WJ-100 had high flocculation efficiency in the conditions of relatively large pH value, temperature, sources of carbon, shaking speed, mixing speed etc.and showed fine resaerch and application importance.

On recently years,the tuberculosis shows the characteristic of higher incidence、 infection、drug-resistance、and Morbidity。However,the emphasis and difficult is preventing and controling of multidrug resistant tuberculosis.Its nosocomial and societal causes of occuring and prevailing were analyzed and control measures from seven facets were expounded in this article.Those include prevention knowledge widely,strengthen management and supervision of floating population,improve ability of diagnosis and therepy,develop combinasion treatment of traditional chinese medicine and western medicine and other assistant treatment, prevent community and nosocomial infection,perfect the drug-resistant surveillance system,carry out the study of new drug 、measure and treatment to anti-tuberculosis actively.

Raising earthworms in boxes with excess sludge is conducted.It is concluded that excess sludge is eatable to earthworms ,and feeding condition is≤30cm filling thickness with fresh excess sludge each time,≤20cm up-filling thickness with rotten-lumpy excess sludge each time.

Dissimilatory Fe(Ⅲ)reduction is the important process in Biogeochemical cycle.This paper gives a systematic introduction to the types of dissimilatory Fe(Ⅲ)reduction,mechanism of insoluble Fe(Ⅲ)oxidizes reduction and the advances of molecular biology in- volved in Fe(Ⅲ)reduction.The status of the applications of dissimilatory Fe(Ⅲ)reduction in environmental contaminant treatment were also discussed.

Bacterial disease is still the important disease hazardous to health of human being and animals.The bacterial mutation technique is an important bacterial research technique,including traditional physical and chemical and biological method,and modern genetic mutation technique.The bacterial genetic mutation technique is one of the research focus in bacteriology nowadays,the mutation strategy varies with different kinds of genetic:mutation technique.The development and application of bacterial mutation technique offers new tools for research on new bacterial vaccine,bacterial genetic function and genetic therapy etc.

A Newcastle disease virus(NDV)field strain SGM01 was isolated from a broiler flock in high antibody level against NDV,and identified by HA,HI cross test and animal regression.SGM01 was determined as a virulent strain with MDT of 50.5h,ICPI of 1.76,IVPI of 2.41 after plaque-purification.The F and HN gene of SGM01 were cloned and sequenced.Analysis of F gene indicated that SGMOI be- longed to genotypeⅦ.The amino acid sequence ~(111)GGRQGRL~(117) in the F protein cleavage site in SGM01 strain is identical to virulent NDV. The homology analysis of F and HN gene sequences compared to reference strains from GenBank indicated that:The F protein amino acid se- quence has homologies of 87.7%～88.3% with published gene typeⅡstrains LaSota and SQZ04 et al.,95.7%～98.2% with genotypeⅦstrains Taiwan95,Yunnan03 et al.,91.8%～91.7% with genotypeⅨstrains F_(48)E_9 et al..Willie HN genes were compared,SGM01 demonstrated a higher homologies of 96.5%～97.2% with strains which were isolated in recent years,but lower homologies of 87.4% and 89.0% with LaSota and F_(48)E_9.

Human bactericidal/permeability-increasing protein(hBPI)cDNA was amplified by reverse transcription(RT)and touchdown PCR(TD-PCR)from blood stem cells collected from healthy human of Uygur nationality in Xinjiang Uygur Autonomous Region of China, and then was subcloned into pEGFP-N1 vector,hBPI cDNA sequence consists of 1,464bp.Comparison with other 4 hBPI cDNA sequences registered in GenBank identified 99% homology in DNA sequence.However,there were two base substitutions(nucleotide 576G→C,nu- clotide 676A→G),one of which resulted in an amino acid(residue 185 Lys→Glu).

ZK-I belongs to Bacillus sp.and shown strong antagonistic abilities to Saccharomyces cerevisiae and several plant pathogens,such as phytophthora sp,and Fusarium oxysporum f.sp.vasinfectum etc.,in this paper,we reported the purification of the antifungal substances and some properities of antifungal compounds.After acid precipitation,silica gel chromatography,C_(18) reversed-phase silica gel chromatogra- phy,we obtained four compounds and knew their peaks were all single by HPLC testing,Combined with MS and HPLC,we concluded that they are the homologous series of compounds,A is jiean-peptide A and the others were confirmed to the analog of Jiean-peptide A in struc- ture.

Optimal parameters of submerged fermentation for Antrodia camphorata were studied.According to the yield of Intracellular triter- pene,the optimal fermentation parameters were obtained as follows:40g/Lglucose,6 g/L soybean,1 g/L K_2HPO_4,0.5 g/L MgSO_4 and 100mg/L VB_1.The optimum cultural period was 6 d at 26℃in 250mL shake flasks at 100r/min.The best aeration ratio was 1:2.5(medi- um volume:flask volume).The inoculation volume was 20%.The intracellular triterpene of Antrodia camphorata was obtained(about 15.25mg/100mL) in the optimum culture.