The interaction of Klebsiella mytoca NG13/pMC73A with Eucalyptus were studied. The results showed that the root surface and even the inner cortex of Eucalyptus were colonized by K. oxytoca. The K. oxytoca was reisolated from rhizosphere, root surface and inner root of inoculated Eucalyptus. The inoculation with K. oxytoca stimulated the excretion of Eucalyptus root and affected the contents of amino acids, carbohydrates, phytohormones of root exudates. The seedlings of Eucalyptusto to inoculation with K. oxytoca increased growth, total dry matter and N content by 29.81% - 100.40% after 3 months in comparinson to the uninoculated seedlings.

The kinetics of growth, lactic acid and nisin production and substrate consumption by Lactococcus lactis subsp. lactis SM526 were studied in different pH. Growth, lactic acid and nisin production were modelled using a simple logistic equation while substrate consumption was foud to be a function of growth and lactic acid production rate. The optimal pH for growth and lactic acid production was 7.0 while it was 6.5 for nisin production according to the models developed.

A new teaching model for food fermentation technology education is proposed in this paper,aiming at improving some of the drawbacks of the traditional teaching method. Some novel approaches,such as summarization,comparison,discussion,as well as bi-lingual lecturing and multi-media assisted teaching,are introduced to inspire students’ passion for learning. Meanwhile,arranging students as dedicated class speaker and organizing students to participate in the exploring experiments also reflect the technology major education characteristics of normal university.

49 rhizobia strains,isolated from root nodules of broadbean(Vicaia faba L.) in 11 provinces of China were studied by analysis of 138 phenotypic characteristics.All the strains collected together at the boundary of 59% similarity.At the similarity of 80% there were 6 groups which were all composed of strains isolated from the root nodules of broadbean except the cluster 4 included one reference strain.Based on the results of numerical taxonomy,25 strains as the representation of the broadbean rhizobia with 11 reference strains were selected to PCR-RFLP of 16S rDNA analysis.The strains were clustered into 4 groups at the similarity of 85% with another 1 unattached embranchment.The cluster status of 16S rDNA PCR-RFLP was in good agreement with that of numerical taxonomy on the similarity level of 80%.Overall the results demonstrated a high phenotypic and phylogenetic diversity of rhizobia strains nodulating broadbean.

A suitable medium,95% wheat bran +5% maize flour + 5% soybean powder,was chosen for mass production of Beauveria bassiana for solid-state fermentation,and the spore count of primary product was up to 244.7?10~(8) conidia per gram.The optimal conditions for solid-state fermentation in a 25m~(3) solid fermentator were as followings.Initial water content of the medium was 60%~65%.The best aeration ratio was: 0 in first 12h,1:2 in 12~48h and 1∶1 after 48h.In order to emit heat released by substantial growth of mycelium,it was necessary to control the temperature from 12h to 48?8h.As to the desiccating process,the optimal condition was with dried air at normal temperature to keep conidia viable.

"Microbiological Examination" is an important core course for medical examination technology major with a strong attribute of practicality and operability. A method of "Cooperation between school and hospital" is employed in the teaching of the course. It is based on the standards for medical professional education required by the hospitals, coupled with our teaching program. In the course of teaching, emphasis is laid on the philosophy of work-learn combination, using methods such as the analysis of individual cases to broaden students’ thinking and develop a sound scientific attitude. By way of "training-on-job learn-ing-interning", we can cultivate students’ ability of working with their own hands, as well as that for analyz-ing and solving problems. In this way, our school will be able to provide "talents with medical examination skill that can be put to practical use" for clinical application.

Lipase from Burkholderia cepacia complex is one of the most versatile biocatalyst and is used widely in many biotechnological application fields including detergent additives, the resolution of racemic compounds, etc. Based on the known whole genomic information of B. cepacia, both ampicillin and kanamycin were added to the TB-T media, the traditional selective media, to screen B. cepacia complex strains from rhizosphere soil samples. The single colonies on the plates with the modified TB-T media were then qualitatively determined the ability to produced the extracellular lipase in the rhodamine B-olive oil agar plates. Thirty-five strains of lipolytic pseudo-B. cepacia complex were isolated and the positive rate of lipolytic bacteria was 65%. Among them, 15 pseudo-B. cepacia complex strains with the tolerance to benzene, n-hexane and n-heptane at the concentration of 10% (V/V) were selected and identified by the recA gene sequence. All of the 15 lipolytic bacteria belonged to the B. cepacia complex.

To construct human tissue inhibitor of metalloproteinase-2 and express it in P.pastoris.The sequence was designed according to the amino acid sequence of the TIMP-2 and high-level expression sequences of P.pastoris.We gained the TIMP-2 gene by artificially synthesis method.The gene was cloned into pPIC9 to construct a recombinant vector pPIC9-T2.The vector was transformed into P.pastoris by electroporation,through phenotype selection and induced with methanol we get the expression cell.Separation,purification and bioactivity analysis of the expressed products was performed.

Determination of the quantum yields of Gibberellin, Hypocrellin A and phenol showed that there were respectively ranges of excitation wavelengths for the relative constant of quantum yields of them. The quantum yields of Gibberellin excited by its own multiple excitaion wavelengths (285 and 410 nm) equaled each other. The quantum yields were similar by excitation of 585 nm and 625 nm with determination of the emission at 640 nm for Hypocrellin A. The emission at 690nm with Hypocrellin A would disappear after it had stood at room temperature for about one month, it suggests that it should be convenient to determine the quantum yield, at a shorter wavelength as for the excitation maximum, of a fluorophore whose concentration was low, weak quantum yield and wavelengths of the excitation and emission locating nearly, with a strong Reilagh scattering; and that the 690 nm emission of Hypocrellin A should be emitted by the groups which were subjected to the effects of physical or chemical disturbences.