2.Wound strength of experimental corneal perforations sealed with fibrin glue in cadaver porcine eyes
Santos Edmin MichaeL G ; Reyes Johann Michael G ; Flores John Vincent PD ; Siong Ruben Lim Bon
Philippine Journal of Ophthalmology 2002;27(2):59-63
Objectives: This is a study to determine the relative wound strength of a commercial fibrin tissue adhesive (Beriplast P, Aventis Behring) compared to 10-0 nylon sutures in sealing experimentally induced corneal perforations in cadaver porcine eyes. Methodology: Single Blind Completely Randomized Design. Sixty freshly enucleatedporcine eyes with an average corneal diameter of 11.5 mm were used. These were randomly allocated into the 3.2 mm perforation group (n = 30) and the 5.2 mm perforation group (n = 30). All perforations were made perpendicular to the corneal surface with a standard preset metal keratome at the central portion of the cornea. Fifteen corneal perforations in each group were again randomly sealed with either interrupted nylon 10-0 sutures or Beriplast P (65-115 mg/mL). Intraocular pressure was raised by injecting normal saline into the anterior chamber and determined by a manometer attached to it. The presealing and postsealing leaking pressures were recorded. Wound leak was determined by Seidels test Results: The mean leaking pressure of the 3.2 mm group sealed with nylon suture was 237.47 mm Hg and 237.33 mm Hg for those sealed with fibrin tissue adhesive. The mean leaking pressures of the 5.2-mm group was 1984 mm Hg for both sealing techniques. The difference in leakingpressures of both sealing techniques was not significant using a power of 80 percent to detect a difference of 30 mm Hg Conclusion: The wound strength of corneal perforations sealed with fibrin tissue adhesive, as measured by the leaking pressures, is comparable to that of those sealed with nylon 10-0 sutures in cadaveric porcine eyes. (Author)
WOUND HEALING
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CORNEAL PERFORATION
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FIBRIN TISSUE ADHESIVE/ADVERSE EFFECT
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CADAVER
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NYLONS
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NYLONS/ADVERSE EFFECT
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NYLONS/CLASSIFICATION
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SUTURES/ADVERSE EFFECT
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EYES
4.Chinese Public Attitudes Toward Epilepsy (PATE) scale: translation and psychometric evaluation
Kheng Seang Lim ; Wan Yuen Choo ; Cathie Wu ; Michael D Hills ; Chong Tin Tan
Neurology Asia 2013;18(3):261-270
Introduction: None of the quantitative scale for public attitudes toward epilepsy was translated to Chinese
language. This study aimed to translate and test the validity and reliability of a Chinese version of the
Public Attitudes Toward Epilepsy (PATE) scale. Methods: The translation was performed according to
standard principles and tested in 140 Chinese-speaking adults aged more than 18 years for psychometric
validation. Results: The items in each domain had similar standard deviations (equal item variance),
ranged from 0.85-0.95 in personal domain and 0.75-1.04 in general domain. The correlation between an
item and its domain was 0.4 and above for all, and higher than the correlation with the other domain.
Multitrait analysis showed the Chinese PATE had a similar variance, floor and ceiling effects, and
relative relationship between the domains, as the original PATE. The Chinese PATE scale showed
a similar correlation with almost all demographic variable except age. Item means were generally
clustered in the factor analysis as hypothesized. The Cronbach’s α values was within acceptable range
(0.773) in the personal domain and satisfactory range (0.693) in the general domain.
Conclusion: The Chinese PATE scale is a validated and reliable translated version in measuring the
public attitudes toward epilepsy.
5.In vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.) extracts
Samad Binte Nadira ; Debnath Trishna ; Ye Michael ; Hasnat Abul Md. ; Lim Ou Beong
Asian Pacific Journal of Tropical Biomedicine 2014;(10):807-815
Objective: To investigate in vitro antioxidant and anti-inflammatory activities of Korean blueberry (Vaccinium corymbosum L.).
Methods:Total phenolic and flavonoid contents of the Korean blueberry water and ethanol extracts were determined before determining the potential of the extracts as antioxidant. Antioxidant activity of the extracts was determined by following some well established methods for free radical scavenging such as 2,2-diphenyl-picrylhydrazyl hydrate, 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid), free radical induced DNA damage, superoxide dismutase-like and catalase assay etc. Furthermore, 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan and nitric oxide assay were performed to determine the anti-inflammatory activity of the extracts.
Results:Total phenolic contents were found (115.0±3.0) and (4.2±3.0) mg GAE/100 g fresh mass for both extracts, respectively and flavonoid contents were (1942.8±7.0) and (1292.1±6.0) mg CE/100g fresh mass for water and ethonal extracts, respectively. Both the extracts displayed significant scavenging activity of some radicals such as 2,2-diphenyl-picrylhydrazyl hydrate (IC50 at 1.8 mg/mL and 2.05 mg/mL, respectively), 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) (IC50 at 1.5 mg/mL and 1.6 mg/mL, respectively) and nitrite (IC50 at 1.7 mg/mL and 1.5 mg/mL, respectively)etc. The extracts were found to prevent inflammation as well by reducing nitric oxide production and cytotoxicity in cell.
Conclusions:The findings suggest that the fresh Korean blueberry could be used as a source of natural antioxidants and anti-inflammatory agents.
6.Developing the "NUS Tummy Dummy", a low-cost simulator to teach medical students to perform the abdominal examination.
Shariff MAHABOOB ; Lian-Kiat LIM ; Chew-Lip NG ; Quan-Yao HO ; Michael E L LEOW ; Erle C H LIM
Annals of the Academy of Medicine, Singapore 2010;39(2):150-151
Abdomen
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physiology
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Humans
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Manikins
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Physical Examination
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methods
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Singapore
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Students, Medical
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Teaching
;
methods
7.Concurrent validity of power output derived from the non-motorised treadmill test in sedentary adults.
Annals of the Academy of Medicine, Singapore 2008;37(4):279-285
INTRODUCTIONMany consider the Wingate Anaerobic Test (WAnT) as a reference standard assessment mode in the measurement of lower limb short-term power output. However the WAnT is criticised for having low ecological validity, in non-cycling tasks and is reliant on a predetermined applied force, which might not elicit the highest power output. A viable alternative to the WAnT is the Non-Motorised Treadmill (NMT) Test, which allows for power measurement in all-out intensity effort sprint-running. With the reliability of the NMT to elicit power in sedentary adults already established, the aim was to compare peak power (PP) and mean power (MP) derived from a 10-s sprint on the NMT to that derived from a 10-s WAnT, to establish concurrent validity.
MATERIALS AND METHODSTwelve male [age 26.6 +/- 2.4 years, body mass (BM) 63.0 +/- 7.2 kg] and 11 female (age 25.3 +/- 3.6 years, BM 51.0 +/- 4.0 kg) sedentary adults participated in the study. PP and MP in absolute, ratio-scaled and allometrically-scaled to BM were analysed. Ratio limits of agreement (LOA) was used to establish the agreement between PP and MP from the NMT and the WAnT.
RESULTSPP in absolute and ratio-scaled to BM from the NMT was between 1.04 and 1.12 times that of PP from the WAnT in 95% of the attempts (PP: NMT, 647.1 +/- 176.4W vs WAnT, 597.0 +/- 146.0W). MP in absolute and ratio-scaled to BM from the NMT test was between 0.88 and 0.97 times of that from the WAnT (MP: NMT, 508.9 +/- 130.7W vs WAnT, 548.7 +/- 131.3W). Power produced on the NMT and the WAnT by sedentary adults shared moderate and acceptable levels of agreement.
CONCLUSIONSThese results affirmed that the NMT could be considered as a viable alternative to the WAnT for the assessment of PP and MP in allout intensity sprint-running lasting 10 s in sedentary adults.
Adult ; Exercise ; physiology ; Exercise Test ; instrumentation ; standards ; Female ; Humans ; Life Style ; Male ; Motor Activity ; physiology ; Muscle Strength ; physiology ; Physical Fitness ; physiology
8.Periprosthetic Hip Joint Infection with Flavonifractor plautii:A Literature Review and Case Report
Alexander WILTON ; Constantine Michael GLEZOS ; Hasitha PANANWALA ; Han Kiong LIM
Hip & Pelvis 2022;34(4):255-261
The purpose of this case report and review of the literature is to provide documentation on periprosthetic hip joint infection with Flavonifractor plautii (formerly known as Eubacterium plautii), a strictly anaerobic bacterium, and to report on a successful pathway for management including staged surgical revisions and extended antibiotic therapy. A systematic review of the literature was conducted, which identified this case as only the fifth documented case of human infection with this organism; as a result, conduct of further research is warranted, based on the paucity of reports in the literature addressing anaerobic periprosthetic joint infection.
10.The use of tissue fiducial markers in improving the accuracy of post-prostatectomy radiotherapy
Michael CHAO ; Huong HO ; Daryl Lim JOON ; Yee CHAN ; Sandra SPENCER ; Michael NG ; Jason WASIAK ; Nathan LAWRENTSCHUK ; Kevin MCMILLAN ; Shomik SENGUPTA ; Alwin TAN ; George KOUFOGIANNIS ; Margaret COKELEK ; Farshad FOROUDI ; Tristan Scott KHONG ; Damien BOLTON
Radiation Oncology Journal 2019;37(1):43-50
PURPOSE: The aim of this retrospective study was to investigate the use of a radiopaque tissue fiducial marker (TFM) in the treatment of prostate cancer patients who undergo post-prostatectomy radiotherapy (PPRT). TFM safety, its role and benefit in quantifying the set-up uncertainties in patients undergoing PPRT image-guided radiotherapy were assessed. MATERIALS AND METHODS: A total of 45 consecutive PPRT patients underwent transperineal implantation of TFM at the level of vesicourethral anastomosis in the retrovesical tissue prior to intensity-modulated radiotherapy. Prostate bed motion was calculated by measuring the position of the TFM relative to the pelvic bony anatomy on daily cone-beam computed tomography. The stability and visibility of the TFM were assessed in the initial 10 patients. RESULTS: No postoperative complications were recorded. A total of 3,500 images were analysed. The calculated prostate bed motion for bony landmark matching relative to TFM were 2.25 mm in the left-right, 5.89 mm in the superior-inferior, and 6.59 mm in the anterior-posterior directions. A significant 36% reduction in the mean volume of rectum receiving 70 Gy (rV₇₀) was achieved for a uniform planning target volume (PTV) margin of 7 mm compared with the Australian and New Zealand Faculty of Radiation Oncology Genito-Urinary Group recommended PTV margin of 10 mm. CONCLUSION: The use of TFM was safe and can potentially eliminate set-up errors associated with bony landmark matching, thereby allowing for tighter PTV margins and a consequent favourable reduction in dose delivered to the bladder and rectum, with potential improvements in toxicities.
Clothing
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Cone-Beam Computed Tomography
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Fiducial Markers
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Humans
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New Zealand
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Postoperative Complications
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Prostate
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Prostatectomy
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Prostatic Neoplasms
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Radiation Oncology
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Radiotherapy
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Radiotherapy, Image-Guided
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Radiotherapy, Intensity-Modulated
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Rectum
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Retrospective Studies
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Urinary Bladder