Background: Continuation of previous study of screening traditional medicinal plants with anti-diabetic effect, the hypoglycemic effect of glue extracted from Fructus Lycii which was named HPD was study. Objective: to investigate the hypoglycemic effect of HPD on diabetic mice caused by different doses of streptozocine. Subjects and methods: causing diabetes by streptozocine at difference doses was used to collect glucose intolerance mice, mild diabetic mice and severe diabetic mice. The effect of HPD p.o. at several doses was evaluated after 3 days of use. Results: By oral allocation with the dose of 500 and 1.000mg/kg body-weight for 3 days, HPD inhibited increasing blood glucose in streptozocine-induced glucose intolerance mice (intraperitoneal STZ 100 mg/kg). HPD 1000 mg/kg body weight had the potent hypoglycemic effect on streptozocine-induced diabetic mice, reverse the blood glucose self control ability of mild streptozicine-induced diabetic mice (STZ 120 mg/kg), meanwhile can not stop a hyperglycemic effect of high dose of STZ (STZ 180 mg/kg). Conclusion: HPD has the potent anti-hyperglycemic effect on streptozocine -induced diabetic rats. Anti-hyperglycemic of HPD is indirect under present of insulin\r\n', u'\r\n', u'\r\n', u'
Cornus ; Mice ; Inbred NOD ; Streptozocine ;

Animals ; Copper ; toxicity ; Disulfiram ; toxicity ; Humans ; Leukemia, Myeloid, Acute ; Mice ; Mice, Inbred NOD ; Mice, SCID

OBJECTIVE: To investigate the tumorigenicity of several multiple myeloma (MM) cell lines transplanted in mice without γ-ray irradiation and to construct the MM disease model to facilitate in vivo experiments. METHODS: NOD/SCID or NSG mice were subcutaneously or caudally transplanted with MM cell lines (LP-1, OPM2, RPMI 8226 and MOLP8), or cell lines with luciferase (RPMI-Luc-Puro, RPMI-Luc-mCherry and MOLP8-Luc-Puro). Tumor growth was observed by measuring the tumor size with a caliper. CD138 tumor cells in peripheral blood were detected by flow cytometry. The free light chain in mouse serum was detected by immunofixation electrophoresis. Tumor type was identified by immunohistochemistry. RESULTS: Twenty one NOD/SCID mice were subcutaneously transplanted with LP-1 cells or OPM2 cells respectively, and no tumors formed till 7 weeks after transplantation. Fifteen NOD/SCID mice subcutaneously transplanted with RPMI 8226 cells showed tumor formation one week later. As of 7 weeks, the rate of tumorigenesis was 80% (12/15). Serum λ light chain was detected and no CD138 tumor cells were detected in peripheral blood. Two NOD/SCID mice each were subcutaneously transplanted with RPMI-Luc-Puro, RPMI-Luc-mcherry and MOLP8-Luc-Puro cells respectively. No tumor signal was detected through IVIS in RPMI-Luc-mcherry cells-transplanted mice. There was tumor signal at 1 week in RPMI-Luc-Puro and MOLP8-Luc-Puro cells-transplanted mice, the former disappeared at 2 weeks and the latter persisted more than 3 weeks. NSG mice subcutaneously transplanted with both cells persistently displayed the tumor signal. Neither NOD/SCID nor NSG mice transplanted with RPMI 8226, RPMI-Luc-Puro, RPMI-Luc-mcherry or MOLP8-Luc-Puro cells through tail vein developed the tumor signal. Only one NSG mice transplanted with MOLP8-Luc-Puro cells appeared transient tumor signal. CONCLUSION Unirradiated mice transplanted with MM cell lines tended to develop local tumor, and failed to develop disseminated tumor. The tumorigenicity of different cell lines is quite different and the vector transfection can reduce the tumorigenic ability. NSG mice with more severe immunodeficiency are more suitable for tumor growth.
Animals ; Carcinogenesis ; Cell Line ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Multiple Myeloma

An abnormal swelling was identified in the distal portion of the right femur in a 1-year-old non-obese diabetic (NOD) mouse. Grossly, a large mass of the distal femur was observed in the right femur. Lesions were poorly marginated, associated with destruction of the cancellous and cortical elements of the bone, and showed ossification within the soft tissue component. Histologically, the tumor was identified as a poorly differentiated sarcoma. Histopathologic examination of the bone masses revealed invasive proliferation of poorly differentiated neoplastic mesenchymal cells forming streams, bundles, and nests, which resulted in destruction of normal bone. Neoplastic cells exhibited random variation in cellular appearance and arrangement, as well as matrix composition and abundance. Haphazard and often intermingling patterns of osteogenic, chondroblastic, lipoblastic, and angiogenic tissues were present. Larger areas of neoplastic bone and hyaline cartilage contained multiple large areas of hemorrhage and necrosis bordered by neoplastic cells. The mass was diagnosed as an osteosarcoma. To our knowledge, this is the first spontaneous osteosarcoma in an NOD mouse.
Animals ; Chondrocytes ; Durapatite ; Femur ; Hemorrhage ; Hyaline Cartilage ; Mice ; Mice, Inbred NOD ; Necrosis ; Osteosarcoma ; Rivers ; Sarcoma

By the concept that IDDM (insulin-dependent diabetes mellitus) is one of the autoimmune diseases, several immune suppressive drugs and immune modulators including BCG have been used to suppress the occurrence of IDDM. A data reported that BCG has different effects for prevention of diabetes according to the timing of drug administration. This study was performed to examine the preventive effect of diabetes development and insulitis by administering BCG at breast-fed period. NOD (non-obese diabetic) mice were divided into three groups: Group I, II, III were injected by BCG on the first day, eighth day, and twenty second day of life respectively. The later BCG was injected, the smaller occurrence of diabetes and the lower severity of insulitis were.
Animals ; Autoimmune Diseases ; Diabetes Mellitus, Type 1 ; Mice ; Mice, Inbred NOD* ; Mycobacterium bovis*

OBJECTIVETo compare the tumor-forming rate between the SCID and NOD/SCID mice to set up acute myeloid leukemia (AML) mouse model.

METHODSThe SCID and NOD/SCID mice were injected with HL-60 cells in to the abdominal cavity in order to contruct the AML mouse model. The gereral status of mice was observed, the positive rate of CD33 in bone marrow cells was detected by flow cytometry, the mouse model was identified by pathologic examination.

RESULTSThe tumor-forming rate in constructed model using SCID mouse was 30%, while the tumor-forming rate in constructed model using NOD/SCID mouse was 100%.

CONCLUSIONCompared with SCID mice, the tumor-forming rate in NOD/SCID mice injected with HL-60 cells is high, the incidence of AML is stable, suggesting that the NOD/SCID mouse model is more suitable to explore the pathogenesis of leukemia.

Animals ; Disease Models, Animal ; Flow Cytometry ; HL-60 Cells ; Humans ; Leukemia, Myeloid, Acute ; Mice ; Mice, Inbred NOD ; Mice, SCID

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive leukemia. However the poor prognosis and low morbidity restrict further analysis of the disease. Therefore there is an increasing demand to develop animal models for identifying novel therapeutic approaches. In this study, we inoculated the anti-mouse CD122 monoclonal antibody conditioned NOD/SCID mice with the leukemia cells from 9 T-ALL patients and 1 cell line via the tail vein. Four of the 9 patients and the cell line were successfully engrafted. Flow cytometry detected high percentage of human CD45(+) cells in recipient mice. Immunohistochemistry showed infiltration of human CD45(+) cells in different organs. Serial transplantation was also achieved. In vivo drug treatment showed that dexamethasone could extend survival, which was consistent with clinical observation. These results demonstrated that we successfully established 5 xenotransplantation models of T-ALL in anti-mCD122 mAb conditioned NOD/SCID mice, which recapitulated the characteristics of original disease.
Animals ; Disease Models, Animal ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; Transplantation, Heterologous ; methods

NOD/SCID (non-obese diabetic/severe combined immunodeficient) mice are immune deficient mice which are made by backcross of severe combined immunodeficient mice with non-obese diabetic mice strains. NOD/SCID mice are both innate immune deficiencies and lack of T and B lymphocytes. Various tumor cells can be implanted in this kind of mice, the rejection and graft-versus-host disease (GVHD) occur fewer. Therefore, NOD/SCID mice gradually become a useful tool for the study on Experimental Hematology. This paper comprehensively reviews the biological characteristics of NOD/SCID mice, the establishment of human leukemia model, stem cell transplantation, drug research, deficiency and improvement of NOD/SCID mice in application for study.
Animals ; Disease Models, Animal ; Hematology ; methods ; Leukemia ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Models, Animal ; Research ; trends

OBJECTIVE: To investigate the expression of cell division cycle protein 37 (Cdc37) in multiple myeloma (MM) and its effect on MM cell proliferation. METHODS: The expression of Cdc37 mRNA in CD138 RESULTS: Cdc37 was highly expressed in newly diagnosed CD138 CONCLUSION Cdc37 is highly expressed in newly diagnosed MM patients. Inhibition of Cdc37 results in decreased proliferation activity and G
Animals ; Apoptosis ; Cell Cycle Proteins ; Cell Proliferation ; Chaperonins ; Humans ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Multiple Myeloma

BACKGROUND AND OBJECTIVEWith the ongoing need to improve therapy for lung cancer, there has been an increasing interest in the development of reliable preclinical models to test novel therapeutics. The aim of this study is to establish a patient-derived lung cancer xenograft model in mice and to observe the biological characteristics of xenografts.

METHODSSurgically resected tumor specimens from patients with lung cancer were implanted in the subcutaneous layer of the NOD/ SCID mice. Cancer specimens of percutaneous lung biopsy by CT fluoroscopy were implanted into the subrenal capsule of nude mouse. The subcutaneous carcinoma was surgically removed when it grew to approximately 1.0 cm in diameter, and then re-transplanted into new nude mice. The growth process of transplanted tumor was observed. Expression of CEA, cytokeratin, and Ki67 were detected by immunohistochemistry. Mutations in the exons 18-21 of EGFR and exons 12,59 of K-ras of primary and xenograft tumors were examined. The cell cycle of xenograft tumor cells was analyzed by flow cytometry.

RESULTSEleven cases were conducted for NOD/SCID mice and nude mice modelling. The patient-derived lung cancer xenografts have been established successfully, and the tumor could be passed to new nude mice, including No 2 model (adenocasinoma), No. 3 model (small cell lung cancer), and No.5 model (squamous cell cancer). High homogeneity was found between xenograft tumors and human lung cancer in histopathology, immunohistochemical phenotype, and EGFR, K-ras mutation status. The S-phase fraction of xenograft cell cycle was prolonged, which indicated that the xenografts remains highly proliferated.

CONCLUSIONThe xenotransplantation models established for patient-derived lung cancer in immune deficient mice. The success rate is 27%. This model system displayed the biological characteristics of human lung cancer, suggesting that it may provide a stable, reliable, and useful animal model in human lung cancer research.

Animals ; Disease Models, Animal ; Female ; Humans ; Lung Neoplasms ; genetics ; pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred NOD ; Mice, SCID ; Middle Aged ; Neoplasm Transplantation ; Transplantation, Heterologous