1.Expression of interferon-induced protein with tetratricopetide repeats 1 and liver cell apoptosis in mice with severe burns.
Xiaoshu GUO ; Jiping GONG ; Song WANG ; Yuhui HAO ; Yongli CHANG ; Chen LI
Journal of Central South University(Medical Sciences) 2015;40(6):598-604
OBJECTIVE:
To explore the relationship between the expression of interferon-induced protein with tetratricopetide repeats 1 (IFIT1) and liver cell apoptosis in the acute stress period after severe burns.
METHODS:
A total of 25 C57/129 adult mice were randomly divided into the normal control group (0 h) and the groups at 1, 6, 12 or 24 after severe burns (n=5 per group). A model with third degree (20% of the total body surface area) burn injury was established and then liver tissues were taken. IFIT1 expression was examined by Western blot. The expression of caspase-3 and -8 was measured by immunohistochemistry. Liver cell apoptosis was detected by terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL).
RESULTS:
After burns, IFIT1 expression was increased at 1 h, which reached the highest level at
6 h followed by a decrease at 12 h, which reached minimum level at 24 h. The differences between groups were significant (P<0.01). The caspase-3 and -8 levels significantly increased after burns in a time-dependent manner (P<0.01). Although at 0 h and 1 h there was no significant increase in liver cell apoptosis, the increase reached significance from 6 h to 24 h (P<0.01).
CONCLUSION
The increase in IFIT1 expression after severe burns promotes liver cell apoptosis.
Animals
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Apoptosis
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Blotting, Western
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Burns
;
metabolism
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Carrier Proteins
;
metabolism
;
Caspase 3
;
metabolism
;
Caspase 8
;
metabolism
;
Hepatocytes
;
cytology
;
In Situ Nick-End Labeling
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Liver
;
cytology
;
Mice
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Mice, 129 Strain
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Mice, Inbred C57BL
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RNA-Binding Proteins
2.Rdh13 deficiency weakens carbon tetrachloride-induced liver injury by regulating Spot14 and Cyp2e1 expression levels.
Xiaofang CUI ; Benting MA ; Yan WANG ; Yan CHEN ; Chunling SHEN ; Ying KUANG ; Jian FEI ; Lungen LU ; Zhugang WANG
Frontiers of Medicine 2019;13(1):104-111
Mitochondrion-localized retinol dehydrogenase 13 (Rdh13) is a short-chain dehydrogenase/reductase involved in vitamin A metabolism in both humans and mice. We previously generated Rdh13 knockout mice and showed that Rdh13 deficiency causes severe acute retinal light damage. In this study, considering that Rdh13 is highly expressed in mouse liver, we further evaluated the potential effect of Rdh13 on liver injury induced by carbon tetrachloride (CCl). Although Rdh13 deficiency showed no significant effect on liver histology and physiological functions under regular culture, the Rdh13 mice displayed an attenuated response to CCl-induced liver injury. Their livers also exhibited less histological changes and contained lower levels of liver-related metabolism enzymes compared with the livers of wild-type (WT) mice. Furthermore, the Rdh13 mice had Rdh13 deficiency and thus their liver cells were protected from apoptosis, and the quantity of their proliferative cells became lower than that in WTafter CCl exposure. The ablation of Rdh13 gene decreased the expression levels of thyroid hormone-inducible nuclear protein 14 (Spot14) and cytochrome P450 (Cyp2e1) in the liver, especially after CCl treatment for 48 h. These data suggested that the alleviated liver damage induced by CCl in Rdh13 mice was caused by Cyp2e1 enzymes, which promoted reductive CCl metabolism by altering the status of thyroxine metabolism. This result further implicated Rdh13 as a potential drug target in preventing chemically induced liver injury.
Alcohol Oxidoreductases
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deficiency
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genetics
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Animals
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Carbon Tetrachloride Poisoning
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enzymology
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Chemical and Drug Induced Liver Injury
;
enzymology
;
pathology
;
Cytochrome P-450 CYP2E1
;
metabolism
;
Female
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Immunohistochemistry
;
Liver
;
drug effects
;
enzymology
;
pathology
;
Male
;
Mice
;
Mice, 129 Strain
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Mice, Inbred C57BL
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Mice, Knockout
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Nuclear Proteins
;
metabolism
;
Transcription Factors
;
metabolism