Objective To explore the etiological diagnosis of obscure gastrointestinal bleeding in patients undergoing double-balloon enteroscopy and summarize the nursing strategies.Methods Eighty-six patients with obscure gastrointestinal bleeding underwent double-balloon enteroscopy. Before operation,preparations of surgical stuff and bowel were conducted,during the operation,surgical cooperation was carefully performed and after the surgery and the patients were closely observed for prevention of complications.Results All the patients lived through the enteroscopy without any complications of perforation and bleeding. The positive detection rate was 83.7%.Conclusion Double-balloon enteroscopy is of great accuracy etiologically for the diagnosis of obscure gastrointestinal bleeding. The nursing measures including right evaluation of patients' condition,careful pre-operational, proficient surgical cooperation and close observation are of great significance for the success of double-balloon enteroscopy.