Objective To verify the performance of VITROS 5600 automatic biochemical analyzer.Methods According to laboratory accreditation criteria and performance verification documents of American CLIA'88,the precision,accuracy,liner range,clinical reportable range and biological reference interval were verified for the test of 17 conventional biochemical indicators performed on VITROS 5600 automatic biochemical analyzer.Results The precision、accuracy、liner range、clinical reportable range and biological reference interval of VITROS 5600 automatic biochemical analyzer were all acceptable.Conclusion VITROS 5600 automatic biochemical analyzer could fully meet the requirements in clinical application.