With the understanding of the immune inflammatory response in the pathogenesis of dry eyes, and the limitations of widely used artificial tears and numerous pharmaceuticals and methods to promote tear secretion, clinicians pay more attention to the therapies that can promote tear secretion actively. Acupuncture treatment for dry eye may meet this requirement.

OBJECTIVE:To establish an HPLC method for determining nicardipine hydrochloride(NCD) microspheres in cerebrospinal fluid(CSF) of rabbits by HPLC.METHODS:NCD microspheres were injected into the cistern of rabbits.Cerebrospinal fluid was sampled at different time points and NCD was separated on Agilent-Zorbax column at a flow rate of 1.0 mL?min-1 with column temperature of 30 ℃.The mobile phase consisted of methanol-water(75 :25,pH=6.0) and detection wavelength was set at 236 nm.RESULTS:The linear range of NCD was 2～128 ng?mL-1(r=0.999 8) with recovery of 98.07%.The RSD of intra-day and inter-day were both below 1.47%.The LOD was about 0.5 ng?mL-1.On 3～11 day after medication,the concentration of NCD fluctuated within(14.15?0.37) ng?mL-1.CONCLUSION:The method is simple,rapid and accurate for determination of NCD in cerebrospinal fluid of rabbits.

0.05).Conclusion BNP and hsCRP may be involved in the occurrence and sustainment of atrial fibrillation.The serum levels of BNP and hsCRP may serve as independent indexes of atrial fibrillation.CK-MB may be not involved in the incidence and development of atrial fibrillation.

8-hydroxydeoxyguanosine (8-OHdG) in human urine is a marker reflecting oxidative stress and DNA oxidative damage. People spend 80%-90% of their life indoors; therefore, indoor air quality is directly related to human health. In this paper, the urinary 8-OHdG levels were presented in populations grouped by different demographic characteristics, lifestyle, occupational exposure, and health status, and elucidated indoor pollutants affecting human urinary 8-OHdG level, such as pollutants from outdoor sources, smoking, indoor combustion and cooking fumes, the chemicals in interior decoration materials, and building foundation soils. The article aims to provide a theoretical basis for predicting the impact of indoor air pollution on human health (DNA oxidative damage and related diseases) by measuring the concentration of 8-OHdG in human urine.

Medical histories and clinical data of 54 patients with Sheehan's syndrome (SS) in endocrine department from August 2008 to August 2011 were collected and analyzed retrospectively.47 cases of SS were accompanied by pituitary crisis.19 cases had been misdiagnosed previously.The average period from the obstetric events to diagnosis of SS was ( 12.36 ± 1.28 ) years.Intravenous drip of hydrocortisone was given for rescuing pituitary crisis.The average dosages of hydrocortisone for the first 24 hours and the second 24 hours were ( 83.93 ± 7.73 ) and (42.19 ± 5.13 ) mg respectively. 12 cases of SS presented transient psychosis after the regular dosage of oral glucocorticoid replacement.

Objective To investigate the relationship between the changes of serum total bilirubin(TBil) and hybrid muscle crea-tine kinase isozyme(CK-MB),and its clinical implication in neonates with hypoxic-ischemic encephalopathy (HIE). Methods The levels of TBil and CK - MB were measured in 49 neonates with HIE (15 mild cases, 20 moderate cases and 14 serious cases), and 31 normal neonates on the first day and the fourth day after birth. Results The levels of TBil on the first day after birth were significantly higher than those in normal neonates;the levels of TBil were correlated with the severity of HIE(P

ObjectiveTo evaluate whether the junior high school students reared by their grandparents in 0～3 years old have more emotional and behavioral problems than their counterparts who reared by birth parents since birth. MethodsThis study was carried out to compare emotional and behavioral outcomes,using Youth Self-Report(YSR) between junior high school students reared by their grandparents in 0～3 years old and junior high school students reared by birth parents since their birth. ResultsTwo-sample test showed that the study group had significantly higher raw scores of the YSR sub-scale of somatic complaint[(2.93±2.83) vs (2.00±2.35),t=2.08,P＜0.05],social problems[(3.56±2.20) vs (2.42±1.99),t=3.15,P＜0.01],thought problems[(3.29±3.26) vs (2.16±2.29),t=2.30,P＜0.05],delinquency [(2.77±2.33) vs 2.01±1.60),t=2.19,P＜0.05] ,aggression[(7.89±6.32) vs 5.96±4.66),t=2.01,P＜0.05],externalizing problems [(10.67±8.07) vs (7.97±5.61),t=2.19,P＜0.05]and total problems [(42.64±22.76) vs (33.61±19.25),t=2.47,P＜0.05]than the comparison group. Analysis of covariance (ANCOVA) was used to control the effects of live events stress. The following analyses showed that the two groups showed no significant difference except for social problems (P＜0.05). ConclusionMany factors that affect mental health of the junior high school students. There is a significant differences in the social problems between the junior high school students reared by grandparents from infancy to the toddler stage and their counterparts. The grandparents should pay more attention to help their grandchildren developing social skills in their babyhood.

Objective To observe the effect of cytokine-induced killer (CIK) cells on the apoptosis of human leukemia cells,and explore the role of miR-155 in this process.Methods The cytotoxicity of CIK cells against a variety of leukemic cell lines (NALM-6,Jurkat) was investigated by MTT technique,miR-155 was determined by real time quantitative PCR,and the apoptosis was detected by flow cytometry in NALM-6 and Jurkat cells induced by CIK cells.Psi CHECK2-CEBP/β 3'-UTR containing the binding site of miR-155 was constructed,and then it was transfected into NALM-6 and Jurkat cells.Luciferase activity of CEBP/β (CCAAT/enhancer binding protein beta) was determined with the assistance of dual luciferase report system.Results CIK cells possessed strong cytotoxicity against NALM-6 and Jurkat cells,which was time-dependent and dose-dependent (P ＜ 0.05).CIK cells could increase the expression of miR-155 in NALM-6 cells by (2.87±0.19) fold (t =2.787,P ＜ 0.05),and in Jurkat cells by (1.98±0.25) fold (t =3.513,P ＜ 0.05).Moreover,miR-155 mimics could promote the apoptosis of NALM-6 and Jurkat cells induced by CIK cells (t =4.239,P ＜ 0.05;t =3.565,P ＜ 0.05).However,miR-155 inhibitor might block this process (t =3.772,P ＜ 0.05;t =4.017,P ＜ 0.05).MiR-155 targeted at the site of CEBP/β3'-UTR,and CIK cells could decrease the luciferase activity of NALM-6 cells by (42.89±2.07) ％ (t =3.578,P ＜ 0.05),meanwhile,in Jurkat cells by (37.02±1.95) ％ (t =4.393,P ＜ 0.05).Conclusion CIK cells could enhance human leukemia NALM-6 and Jurkat cells apoptosis by upregulating miR-155,which may provide a new database to elucidate leukemia cell therapy using CIK cells.

Objective To investigate the effect of Atorvastatin on the proliferation and apoptosis of leukemic HL-60-cell line,and to explore the possible role of phosphatidylinositol 3-kinase/serine/threonine protein kinase /mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway in this process.Methods HL-60 cells were incubated with different concentrations of Atorvastatin (1,5,10 μmol/L),and HL-60 cells without any treatment were used as controls.The proliferation of HL-60 which was investigated by four methyl thiazolyl tetrazolium assay when cells were cultured for 12,24,48 hours.The apoptosis was detected by flow cytometry after cells were incubated for 48 hours.The mRNA and protein expressions of AKT,PI3K and mTOR were detected by reverse transcription-polymerase chain reaction and Western blot methods,respectively.Results The results indicated that Atorvastatin could inhibit the proliferation and induce the apoptosis of HL-60 cells.When treated with 10 μmol/L Atorvastatin after 48 h,the proliferation inhibition of HL-60 was observed most obviously,with a high rate of (39.77 ± 3.01) ％,compared with the control group,it had statistical significance (t =4.016,P ＜ 0.01),meanwhile,the apoptosis of HL-60 was most notable,at a rate of (43.29 ±3.91)％,compared with the control group,it had statistical significance (t =3.625,P ＜ 0.05).There were basal expression of AKT,PI3K and mTOR in the control group.When treated with 10 μmol/L Atorvastatin after 48 h,the mRNA expression of PI3K,AKT and mTOR were down-regulated most obviously,at a decrease of (37.05 ± 4.11) ％,(53.79 ± 3.27) ％,(40.63 ± 2.42) ％ (t =4.805,3.799,4.312,all P ＜ 0.05),respectively,in comparison with the control group.At the same condition,the protein expression of PI3K,AKT and mTOR were decreased most visibly,with a decline of (41.09 ± 3.17) ％,(45.67 ± 2.92) ％,(63.41 ± 3.59) ％ (t =3.576,4.727,4.902,all P ＜ 0.05) respectively in comparison with the control group.Conclusions Atorvastatin can inhibit the proliferation and induce the apoptosis of leukemic cell HL-60,and the mechanism may be associated with the PI3K/ AKT/mTOR signal pathway.

To explore the effects of dietary conjugated linoleic acid(CLA) on body fat accumulation in male Wistar rats. The obese rat model was established by feeding high lipid forage. The rats were fed a semi-purified diet containing 0.5,1.0,3.0g?kg -1 CLA for 36 days. Low dose group, middle dose group and high dose group all could remarkably reduce the gain of body weight. Low dose group could reduce the body adipose, middle and high dose group could remarkably decrease the body adipose. There were significant differences in the fat and protein contents in the rats thigh between the control group and the group fed high dose CLA. These results suggested that CLA can reduce the body fat in obese Wistar rats.