Objective: To study the chemical constituents in the roots of Goniothalamus cheliensis. Methods: Silica gel, ODS, and Sephadex LH-20 column chromatographic techniques were used to isolate and purify the chemical constituents and their structures were elucidated by spectral analyses. Results: Seven compounds were isolated and identified as acetylgoniofupyrone A (1), protocatechuic acid (2), pinocembrin (3), pinoresinol (4), goniodiol (5), 8-epi-goniotriol (6), and cardiobutanolide (7). Conclusion: Compound 1 is a new goniofupyrone-type of styryllactone, named acetylgoniofupyrone A.

OBJECTIVE:To observe analgesic efficacy of oxycodone acetaminophen after posterior lumbar fusion,and to opti-mize spinal postoperative analgesia plan. METHODS:In retrospective study,120 patients underwent posterior lumbar fusion were selected from the Affiliated People's Hospital of Hubei Medical College during Jun. 2013-Jun. 2014,and then divided into observa-tion group and control group according to therapy plan,with 60 cases in each group. Observation group was given oxycodone acet-aminophen 330 mg orally 6 h after surgery,and then 330 mg orally on 1st,2nd day after surgery,tid. Control group was given celecoxib 200 mg 6 h after surgery,and then 200 mg on 1st,2nd day after surgery,bid. Postoperative analgesic efficacy (VAS score) at resting state and active state,postoperative gastrointestinal motility recovery (the time to flatus) and the occurrence of ADR were observed in 2 groups after surgery. RESULTS:Whether at the resting state or the active state 24,48,72,120 h after surgery,VAS score of observation group was significantly lower than that of control group,with statistical significance(P<0.05). There was no statistical significance in the time to flatus and the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Oral administration of oxycodone acetaminophen possesses good effect to relieve spinal postoperative pain,showing fast and safe analgesic efficacy.

OBJECTIVE: To investigate the clinical features of primary biliary cirrhosis (PBC) and its treatment by integrated traditional Chinese and western medicine. METHODS: 16 PBC patients were observed. Ursodeoxycholic acid (UDCA) was used in the dose of 13 to 15 mg.kg(-1).d(-1), with some traditional Chinese herb prescription. Results (1) The proportion of women to men was 15:1, the mean age was 52.5 years. AMA-M2 was positive in 14 subjects (87.5%). Biliary tract enzymes and ESR were elevated in all subjects. The ratio of hypercholesterolemia (CHOL) and abnormality in IgM was high (62.5%). Fatigue, pruritus, arthralgia, jaundice, splenomegaly were noted in more than half cases. Every patient had one to four complications. (2) ALP, GGT and Glb declined evidently after 3 months' treatment by western medicine associated with traditional Chinese medicine, and declined markedly after 12 months' treatment (P<0.05 respectively). TBIL and CHOL declined gradually during the treatment course. Symptoms and signs were lightened. CONCLUSION: PBC has complicated and especial clinical features. UDCA therapy is effective in PBC, while traditional Chinese medicine has extraordinary effect in treating the symptoms and signs.

The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.
Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Herbal Medicine ; Humans ; Phytotherapy ; Plants, Medicinal ; chemistry

Objective To explore the relationship between transforming growth factor-β1 (TGF-β1) and obesity-related glomerulopathy (ORG),and to analyze the possible mechanism for ORG and the new approach to its treatment.Methods Based on their body weight,30 SD rats were randomly divided into 2 groups : the normal control group (15 rats) fed with common food and the ORG model group (15 rats) fed with fat-enriched diets.The rats were sacrificed at the end of the 8th week,and their kidneys were taken out.Immunohistochemistry was used to detect TGF-β1 protein expression.Real time (RT)-polymerase chain reaction (PCR) was used to extract and detect the expression of TGF-β1 mRNA,and Western blot was applied to examine the expression of TGF-β1 protein.The findings were analyzed by using SPSS 13.0 software.Results Compared with the control group, qualitative TGF-β1 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in renal tubules and interstitium.The average absorbance value of the control group and the model ORG group was 0.040-0.013,0.171 ± 0.084, respectively.The difference was statistically significant(P ＜ 0.05).The expression of TGF-β1 mRNA detected by RT-PCR was also increased compared with that of the control group(4.4 vs 0.6).The difference was statistically significant(P ＜ 0.05).The protein expression of TGF-β 1 examined by Western blot showed that it was more than that in the control group(4.3 vs 0.4).The difference between the control group and ORG model group was statistically significant(P =0.002).Conclusions The expression of TGF-β 1 in kidneys of ORG model rats increased, which not only indicates it can participate in ORG's occurrence and development, but also provide the basis to find out the mechanism and the approach to treatment.

AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-? 1 in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM TGF-? 1 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-? 1 and reducing excessive accumulation of laminin and type IV collagen.

Objective:To investigate the impact of Puerarin on oxidative stress in patients undergoing continuous ambulatory peritoneal dialysis(CAPD).Methods: Thirty-eight patients CAPD were randomly divided into two groups,an experimental group(n=19) receiving peritoneal dialysis fluid containing Puerarin(50 mg/2L) in vivo for two weeks and a control group(n=19) receiving dialysis fluid without Puerarin.The concentrations of oxidative stress markers in the serum and effluent such as glutathione(GSH),total-superoxide dismutase(T-SOD),malondialdehyde(MDA),Kt/V and creatinine clearance rate of the two groups(Ccr) were measured and compared before and after the treatment.Results: After the treatment,the concentrations of GSH and T-SOD in the serum and effluent were significantly higher(P

Objective To investigate the healthcare-associated infection(HAI) status, common infection sites, and antimicrobial use in a county hospital in 2011-2015.Methods With combination of bedside survey and case history reviewing, HAI among all hospitalized patients at 0:00-24:00 on April 26, 2011, April 25, 2012, May 14, 2013, September 9, 2014, and August 12, 2015 were surveyed, survey data were analyzed by software SPSS17.0.Results A total of 5 165 patients participated in 5 surveys of HAI prevalence rate, 132 patients had 138 cases of HAI, prevalence rate and case prevalence rate were 2.56％ and 2.67％ respectively.Intensive care unit had the highest average HAI prevalence rate(22.58％).Lower respiratory tract was the main HAI site (58.70％), followed by urinary tract (12.32％), upper respiratory tract (10.14％), and skin soft tissue(5.07％).A total of 45 strains of pathogens were isolated, mainly gram-negative bacteria (n=30, 66.67％).The total usage rate of antimicrobial agents was 45.27％, in 5 prevalence surveys were 61.48％, 43.89％, 37.81％, 39.43％, and 44.79％ respectively.The main purpose of antimicrobial use was therapy (including therapy + prevention), accounting for 78.27％;monotherapy accounted for 81.27％.Conclusion Through prevalence rate survey for 5 consecutive years, HAI management departments can be more comprehensively understand HAI in key departments, key patients, key sites of HAI, and antimicrobial use in this hospital, and provide basis for the formulation and implementation of HAI prevention and control measures according to the actual situation of the hospital.

Objective: To obtain a prokaryotic expression vector containing saliva binding region (SBR) gene of Streptococcus mutans. Methods: By directional cloning method, SBR gene fragment was cloned into the expression vector pcMVT7, the recombinant plasmid pcMVT7-SBR was transformed to E.coli JM109 (DE3). The gene expression was induced with IPTG. Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA, and finally target protein was purified by affinity chromatography. Results:The DNA sequence of SBR in the reconstructed vector pcMVT7-SBR was in corresponding with the initial design. The C-terminal 6?His tagged SBR fusion protein was expressed in JM109(DE3) and was purified by affinity chromatography. The expression rate of target protein was 29.73%. Conclusion:The recombinant expression plasmid pcMVT7-SBR was constructed.

Objective To compare the clinical therapeutic efficacy of excision and primary suture after the hydrogen peroxide and methylene blue treatment, and Limberg transposition flap for the treatment of pilonidal sinus. Methods The clinical data of 55 patients with sacrococcygeal pilonidal sinus were analyzed retrospectively. Thirty-six patients were treated by excision and primary suture after the hydrogen peroxide and methylene blue treatment (suture group), and 19 patients were treated by Limberg transposition flap (flap group). The operation time, length of hospital stay, incision healing time, incidence of complications and recurrence rate were compared between 2 groups. Results There were no significant differences in the length of hospital stay, incision healing time, incidence of complications and recurrence rate between 2 groups (P>0.05). The operation time in suture group was significantly shorter than that in flap group: (51.25 ± 7.02) min vs. (71.42 ± 12.29) min, and there was statistical difference (P = 0.000). Conclusions Excision and primary suture after the hydrogen peroxide and methylene blue treatment is a good method for the treatment of pilonidal sinus. The operation time is short. It is easy to operate, and could achieve good results.