1.Effects of Dexamethasone on Changes of CD4~+,CD8~+ Lymphocyte in Children with Viral Encephalitis
yu-miao, ZHANG ; rong, YANG ; fang-jun, LUO ; yi, ZHOU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective To observe the effects of dexamethasone (DEX) therapy on the clinical outcome and changes of lymphocyte subsets in children with mild and severe encephalitis.Methods Eighty-two children with mild and severe viral encephalitis were randomly divided into two groups: with DEX treatment (n=46) or without(n=36).The clinical efficacy was evaluated 3 weeks later, and the clinical manifestation were also observed. The changes of CD4~+,CD8~+ T lymphocyte percentage were determined by flow cytometry on admission and at 1 week,4 weeks after treatment.Another group of 20 cases was enrolled as control group.Results Compared with the control group,both the DEX treatment group and non-DEX treatment group showed a reduced CD4~+ lymphocyte count, an increased CD8~+ lymphocyte count(P
2.The impact of pain, anxiety and depression on sleep quality in Chinese patients with neuromyelitis optica spectrum disorders
Xiaohui Miao ; Ziyan Shi ; Hongxi Chen ; Hongyu Zhou ; Rong Yang
Neurology Asia 2017;22(3):235-241
Background & Objective: Sleep quality in neuromyelitis optica spectrum disorders (NMOSD) were
investigated in two recent studies. However, factors affecting sleep quality have not been studied in
NMOSD. This study aimed to investigate the prevalence of sleep disorders in Chinese outpatient clinics
with NMOSD and its clinical correlates. Methods: We administered Chinese validated self-questionnaires
on HRQOL (MSQOL-54), sleep (PSQI), pain (SF-MPQ-2), anxiety (HARS) and depression (HDRS)
to 42 patients followed up in our outpatient department. We assessed the relationships between sleep
quality with pain, anxiety, depression, gender, age, disability, disease duration, NMO-antibody status
and explored the determinants of poor sleep quality. Results: Sixty four percent of NMOSD patients
were poor sleepers. Significant correlations were found between duration, disability, pain, anxiety,
depression and sleep quality. Disability, depression and the domain of affective descriptors of pain
were the three main predictors of poor sleep in NMOSD.
Conclusion: This study reveals that poor sleep in NMOSD is common and it decreases physical function
of quality of life. It is worthwhile considering exploring adjuvant strategies aimed at controlling pain
associated affect, and treatment of depression may help to improve sleep quality in NMOSD.
Neuromyelitis Optica
3.Correlation between Extended High-frequency Threshold and Distortion Product Evoked Oto Acoustic Emissions Amplitudes
Yan MIAO ; Rong SONG ; Limei YU ; Yiqing CHEN ; Yang ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2012;18(8):701-703
Objective To investigate the correlation between extended high-frequency audiometry and distortion product oto acousticemissions (DPOAE) amplitudes. Methods 60 normal hearing people aged 20~40 years were examined with extended high-frequency audiometry and DPOAE. Results Extended high frequency thresholds negatively correlated to DPOAE. Conclusion There is correlation between these two methods, and they can be used for early creening and diagnosis of hearing loss.
4.The morbidity of pneumoconiosis in Jiaozuo city.
Zhen-rong MIAO ; Xiao-fa YANG ; Lian-xia GUO ; Shu-xia SHI ; Fang-li DU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):298-299
Adult
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China
;
epidemiology
;
Coal Mining
;
Humans
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Middle Aged
;
Morbidity
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Pneumoconiosis
;
epidemiology
5.Influence of TIEG1 on apoptosis of HL-60 cells and expression of Bcl-2/Bax.
Kun YAO ; Ying YANG ; Rong HU ; Miao MIAO ; Ai-Jun LIAO ; Wei YANG ; Zhuo-Gang LIU
Journal of Experimental Hematology 2013;21(3):587-590
This study was aimed to investigate the influence of TIEG1 on apoptosis of HL-60 cells and the expression of Bcl-2/Bax. Different concentration of TIEG1 were used to treat HL-60 cells, the cell growth inhibition rate was detected by MTT method. After treating HL-60 cells with 12.03 ng/ml TIEG1, cell apoptosis was detected with flow cytometry. Bcl-2 and Bax was detected with RT-PCR. The results showed that TIEG1 had inhibitory effect on HL-60 cell proliferation, and in time-and dose-dependent manners. The more obvious inhibitory effect was observed in HL-60 cells treated with TIEG1 of 12.03 ng/ml. During the course of cell apoptosis, Bax expression increased, but Bcl-2 expression decreased (P < 0.05). It is concluded that TIEG1 inhibits HL-60 cell proliferation and induces apoptosis in time and dose-dependent manners. During the course of HL-60 cells apoptosis induced by TIEG1, Bcl-2/Bax are associated with HL-60 cell apoptosis induced by TIEG1.
Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Early Growth Response Transcription Factors
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pharmacology
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Gene Expression Regulation, Leukemic
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HL-60 Cells
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Humans
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Kruppel-Like Transcription Factors
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pharmacology
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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bcl-2-Associated X Protein
;
metabolism
6.Effect of valproic acid sodium on proliferation and apoptosis of acute T-lymphoblastic leukemia Jurkat cells.
Miao MIAO ; Bing DU ; Rong HU ; Ying YANG ; Wei YANG ; Ai-Jun LIAO ; Zhuo-Gang LIU
Journal of Experimental Hematology 2013;21(2):343-346
This study was aimed to investigate the effects of valproic acid sodium (VPA) on the proliferation and apoptosis of acute T-lymphoblastic leukemia Jurkat cells. Jurkat cells were treated with different concentration of VPA. Proliferation-inhibition curve was assayed and plotted by CCK-8 method and the cell apoptosis was detected by flow cytometry with Annexin V/PI double staining. The expression level of anti-apoptotic gene BCL-2 and pro-apoptosis gene Bak1 were detected by semi-quantitative RT-PCR. The results showed that the VPA inhibited the proliferation of Jurkat cells in concentration-dependent manner. As compared with the control group, the apoptosis of cells increased along with adding concentration of VPA; VPA could decrease the expression of BCL-2 gene, but did not show obvious effect on the expression of Bak1. It is concluded that the VPA can inhibit proliferation of Jurkat cells which possibly associates with the decrease of BCL-2 expression.
Apoptosis
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drug effects
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Cell Proliferation
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drug effects
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Humans
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Jurkat Cells
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Proto-Oncogene Proteins c-bcl-2
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metabolism
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Sodium
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pharmacology
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Valproic Acid
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pharmacology
;
bcl-2 Homologous Antagonist-Killer Protein
;
metabolism
7.Methylation in Promoter Region of SLC6A2 Gene in Heart Failure Patients and Its Correlation with Qi Deficiency/Blood Stasis Syndrome.
Jie-wei LUO ; Xiao-rong MENG ; Fang-meng HUANG ; Dan HU ; Xiao YANG ; Xing-yu ZHENG ; Shi-chao WEI ; Miao-miao GUO ; Shi-ming WU ; Hong-yi CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1448-1454
OBJECTIVETo explore the methylation status in promoter region of norepinephrine transporter gene (NET, SLC6A2) in heart failure ( HF) patients and its correlation with qi deficiency/blood stasis syndrome (QDS/BSS).
METHODSThirty-six patients with heart failure (NYHA classification III to IV) were recruited in the study (as the heart failure group) and their scores of QDS/BSS were evaluated. Besides, a healthy elderly group (30 cases) and a healthy youth group (30 cases) were also set up. They were recruited from Physical Examination Center of Fujian Provincial Hospital. Pyrosequencing was applied to detect the methylation in promoter region of SLC6A2 gene, and the total methylation index (MTI) of CpG island was calculated. The correlation between the methylation status in promoter region of SLC6A2 and scores of QDS/BSS was assessed using Pearson and Partial analyses. Risk factors were screened and adjusted using Logistic regression.
RESULTSBy one-factor analysis of variance, the total MTI in the HF group (219.72% ± 54.03%) was obviously higher than that in the healthy elderly group (194.47% ± 34.92%) and the healthy youth group (161.60% ± 41.11%) (all P < 0.05). Meanwhile, the total MTI was higher in the healthy elderly group than in the healthy youth group (P < 0.01). By covariance analysis , after controlling age and BMI, the total MTI was higher in the HF group than in the healthy elderly group (P = 0.041), while it was higher in the healthy elderly group than in the healthy youth group (P = 0.016). Age was found to play an essential role in affecting MTI of SLC6A2 gene promoter region among the 3 groups (F = 16.447, P = 0.01). The total MTI was quite lower in the healthy youth group. Results of Partial correlation analysis showed MTI was positively correlated with scores of qi deficiency and blood stasis respectively (r = 0.494 and 0.419 respectively, both P < 0.05). Logistic regression analysis showed after adjusting confounding factors, the relative risk (OR value) of total MTI of SLC6A2 gene in promoter region was 1.038 (95% CI, 1.006 to 1.071, P = 0.020).
CONCLUSIONSAbnormally elevated methylation of the promoter region of SLC6A2 gene is one of risk factors for HF. In addition, the degree of methylation of the promoter region of SLC6A2 gene was positively correlated with the severity of QDS/BSS.
Adolescent ; Aged ; DNA Methylation ; Heart Failure ; genetics ; physiopathology ; Humans ; Logistic Models ; Medicine, Chinese Traditional ; Norepinephrine Plasma Membrane Transport Proteins ; genetics ; Promoter Regions, Genetic ; Qi
8.Advances in carbapenemase detection in Gram-negtive bacilli
Chenrui HOU ; Yun YANG ; Ziyang LI ; Ruixue WANG ; Chunyan GAO ; Lili DING ; Miao SU ; Chaojun LANG ; Jianrong RONG
Chinese Journal of Clinical Infectious Diseases 2016;9(3):248-254
With increasing use of carbapenem antibiotics , carbapenems-resistant gram-negative bacteria are spreading, and carbapenemase-producing is the main mechanism of carbapenems resistance . Rapid and accurate identification of carbapenemase and its type is of great importance to timely and effective treatment and control of infections .Chromogenic /Fluorogenic culture media, modified Hodge test and double disk synergy test are traditional methods for carbapenemase detection , but all are time-consuming. Biochemical method is more time efficient and with high sensitivity and specificity , but cannot be used to identify subtypes.Now matrix-assisted laser desorption ionization -time-of-flight mass spectrometry (MALDI-TOF MS) has been successfully applied in the identification of species , subtypes and detection of drug -resistant genes.And among various carbapenemase gene detection techniques , next generation sequencing (NGS) can also be used for the detection of integrons , transposons and plasmids, which is important in both epidemiology and resistant mechanism studies .This article reviews the advantages and disadvantages of various methods for phenotype and gene detection of carbapenemase .
9.Simultaneous determination of resveratrol, emodin, chrysophanol, physcion, in root of Polygonam cuspidatum and its extract by HPLC.
Hong-Mei YANG ; Bo CHEN ; Jian-Guo ZENG ; Jian-Rong MIAO
China Journal of Chinese Materia Medica 2006;31(3):202-205
OBJECTIVETo establish a HPLC method for simultaneous determination of resveratrol, emodin, chrysophanol, physcion in root of Polygonum cuspidatum and its extract.
METHODThe separation was performed in a Hypersil ODS C18 column with a mobile phase of Acetonitrile-1%HAc. The flow rate was 1.0 mL x min(-1) and detection wavelength was at 287 nm.
RESULTThe mean recovery and RSD of resveratrol, emodin, chrysophanol, physcion was 101.8%, RSD 1.7%; 96.2%, RSD 1.4%; 99.7%, RSD 1.0%; 98.60%, RSD 0.97% respectively.
CONCLUSIONThe method is accurate, simple and reliable. It can be used for quality control of P. cuspidatum and its extract.
Anthraquinones ; analysis ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Emodin ; analogs & derivatives ; analysis ; Fallopia japonica ; chemistry ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control ; Stilbenes ; analysis
10.Synthesis and antibacterial activity evaluation of octapeptin derivatives
He-xian YANG ; A-long CUI ; Yong-jian WANG ; Shi-bo KOU ; Miao LÜ ; Hong YI ; Zhuo-rong LI
Acta Pharmaceutica Sinica 2024;58(1):152-160
Octapeptin has strong antibacterial activity against Gram-negative bacteria such as