Objective To explore the spatial distribution of Keshan disease(KD) in the 15 surveillance provinces(municipalities,autonomous regions ) and to provide the basis for the development of prevention and control strategies.MethodsBased on the KD surveillance data of the 15 provinces in 2007,five indicators were selected.Moreover,a comprehensive indicator score to assess KD of different areas was made through the method of principle components analysis,which was applied for regionalization of the KD areas by the subsection method of standard deviation in whole China.The KD areas were divided into mild,moderate and severe endemic areas.The spatial distribution feature of the comprehensive indicator score was displayed by using geographic information system (GIS).ResultsThe three principal components contained 88.123％ information of all the selected indicators,the first principal component had a close relationship with total KD detection rate,chronic KD detection rate and latent KD detection rate; the second principal component had a close relationship with the threatened number in KD areas,and the third principal component had a close relationship with new KD detection rate; the comprehensive indicator indicates that Gansu,Jilin,Heilongjiang,Hebei,and Liaoning provinces were serious prevalent KD areas; Inner Mongolia,Shandong,Hubei,Sichuang provinces (autonomous region) were moderate prevalent KD areas,and Shanxi,Shaanxi,Yunnan,Henan,Guizhou,Chongqing provinces(municipality)were mild prevalent KD areas.Conclusions The introduction of the GIS to Keshan disease monitoring,provides a convenient and direct method to observe the spatial distribution of the disease,and the results point out the key areas for further KD surveillance according to local conditions.

Objective To investigate the expression of HSF2 in colonic mucosa of patients with ulcerative colitis (UC),Crohn's disease (CD),intestinal tuberculosis (ITB),intestinal lymphoma (IL),infectious enteritis,Behcet's disease and normal control.Methods Intestinal tissue samples were retrieved from 2003-2011 archived specimen at the Department of Pathology,and assigned to UC group (n =38),CD group (n =29),ITB group (n =31),IL group (n =32),infectious enteritis group (n =32) and Behcet's disease group (n =28).10 cases were recruited as normal control group.The expression of HSF2 in colonic mucosa were detected by immunohistochemistry.Positive cells were counted by Image Analysis.Results The expression rate of HSF2 in intestinal mucosa of UC patients (64.64 ± 15.17) was significantly higher than that of CD (32.44 ± 5.94),ITB (36.93 ± 6.32),IL (36.16 ± 6.55),infectious enteritis (37.86 ±7.76),Behcet's disease (34.90 ±5.92) and normal control (35.54 ±6.76) (P ＜0.05),while there was no significant difference among the latter six groups (P ＞ 0.05).Conclusion HSF2 is closely related with UC,and may play an important role in the pathogenesis,diagnosis and differential diagnosis of UC.

Objective To evaluate medium-term results in critical limb ischemia (CLI) patients undergoing endovascularly revascularization based on concepts of angiosomes.Methods Clinical data were reviewed on 612 CLI patients who had isolated below-the-knee (BTK)lesions and were treated by endovascular technology from June 2006 to May 2012.Following the angiosome model of perfusion,286 patients (357 limbs)were treated by directly revascularizing the feeding artery.326 patients (455 limbs)were treated by revascularizing non-feeding artery to improve perfusion to ulcer indirectly.Primary endpoints included ulce-healing rate as of 3 months,amputation-free survival( AFS),freedom from major amputation (FFMA) and recurrence rate of CLI.Results During follow-up(mean,24 ± 18 months),ulce-healing rate during 3 months of direct treatment group was obviously higher than that of indirect group (82.4％ vs.66.4％,X2 =26.16,P ＜0.05).The fourth year AFS was 48％ ±5％ vs.37％ ±6％,t =3.44,P ＜0.05,and the fourth year FFMA was 73％ ±4％ vs.58％ ±3％,t =4.61,P ＜0.05.In recurrence rate of CLI,there wasn't significant difference between direct group and indirect group(42％ ±5％ vs.45％ ±6％,t =- 0.64,P ＞ 0.05 ).Conclusions Following the angiosome model of perfusion,direct revascularizing the feeding artery of CLI patients can obviously improve ulcer-healing rate during 3 months,medium-term AFS and FFMA.

There are many ways to prepare middle cerebral artery occlusion (MCAO) rat model. The focal cerebral ischemia model es-tablished by the suture-occluded method is widely accepted as an ideal focal cerebral ischemia model, which can be used in the basic and clinical study of cerebral vascular diseases. However, it still has the defects of high technical requirement, large model difference and high animal mortality. A variety of focal cerebral ischemia models were established by changing the retention time of the thread, the road the thread plugging into, the type of thread bolt and the depth of the thread plugging. This article compared different suture-occluded method, as well as the suture-occluded method to other modeling methods, different cerebral ischemia models may be different in the pathophysiology of cerebral infarction, and the effects may also be different.

Objective The objective of this study was to explore the value of enhanced recovery surgery(ERAS)in perioperative period of laparoscopic assisted colorectal cancer surgery.Methods From November 2015 to July 2016,86 patients with colorectal cancer treated with colorectal surgery were divided into ERAS group and routine treatment group.All patients underwent laparoscopic radical resection of colorectal cancer.The operative situation,postoperative recovery and complications were analyzed in this study.Results There was no difference in the operation time and bleeding volume between the operation group and the conventional treatment group(P>0.05).The postoperative ventilation and defecation time in the ERAS group were earlier than those in the routine treatment group.The postoperative hospital stay and the postoperative pain score were lower than those in the routine treatment group.The rate of postoperative complications was lower than that in the routine treatment group(P<0.05).Conclusion The operation of laparoscopic colorectal cancer under the guidance of ERAS can help patients to recover from hospital,shorten the hospitalization time and reduce the cost of hospitalization.The further application of ERAS should be combined with various disciplines.

Imaging techniques play a vital role in evaluating myocardial damage in patients with myocardial infarction. Accurate evaluations of postinfarction function and scar can help identify high-risk patients and provide prognosis information, which contributes much to clinical practice. The assessment of myocardial damage mainly includes overall evaluations of ventricular function and remodeling and targeted characterization of myocardial infarction including infarct size, myocardial viability and microvascular obstruction. Echocardiography, cardiac magnetic resonance, CT and nuclear examinations are most common imaging techniques currently. This review is to update evidence on applications of these modalities in evaluation of postinfarction myocardial damage and offer some helps to health workers.

Objective To explore the effects of early exercise on motor function and the mechanism in rats after focal cerebral ischemia. Methods 21 Sprague-Dawley male rats were divided into control group (n=7), natural recovery group (n=7) and exercise training group (n=7), and the latter 2 groups were modeled with middle cerebral artery occlusion. The exercise training group received wheel running exercise 24 hours after modeling for 14 days. They were assessed with Rogers score and beam balance test 21 days after modeling. 4 rats in each group received routine MRI and diffusion tensor imaging (DTI) scanning. The fractional anisotropy (FA) and apparent diffusion coefficient (ADC) of ischemic (L) and contralateral side (R) were measured, and the rate of them (L/R, r) was calculated. Results There was significant difference in Rogers score among the groups (Z=-0.786, P<0.001), but no significant difference between the natural recovery group and the exercise training group (P>0.05). Beam balance score of the natural recovery group was significantly different from the control group and the exercise training group (F=38.11, P<0.001), but there was no significant difference between the latters (P>0.05). The LFA of the natural recovery group was significantly different from the control group (P<0.05), but not significantly different from the exercise training group (P>0.05);while the rFA of the natural recovery group was significantly different from the control group and the exercise training group (F=19.30, P<0.05). LADC and rADC might be different between the control group and the natural recovery group (P=0.056, P=0.057), but not significantly different between the natural recovery group and the exercise training group (P>0.05). LFA and rFA strongly correlated with all the behavior scores (r=-0.90--0.70, P<0.01); while LADC and rADC moderately correlated with the behavior scores (r=0.50-0.75, P<0.05), except balance (P>0.05). DTT showed that the direction and density of neural fibers were significantly asymmetrical in the natural re-covery group and the exercise training group, and more symmetrical in the exercise training group. Conclusion Early exercise can promote the neural functional recovery in rats after focal cerebral ischemia, which may associate with the improve of conduction of fibers.

OBJECTIVE To solve the problem of the dynamic disinfection when repollution of the air in the key(departments) of hospital exists.METHODS The germicidal effecacy using the dynamic and undynamic disinfection of Kangaking Air Disinfector in the key departments was detected.RESULTS The total air germ was probably zero after undynamic infection.(After) dynamic infection,the total air germ conformed the national standardⅡand Ⅲ.Many kinds of department′s rate of infection descended than ever.CONCLUSIONS The effective disinfection of Kangaking YKX/G,YDX/Y Type Air Disinfector is obvious.The disinfector is a new anti-infective method and one of the most important way to prevent the hospital infection.

SUMOylation is a dynamically reversible process that needs to be modified by a specific ligase, while its inverse reaction deSUMOylation is catalyzed by a group of SUMO-specific proteases (SENPs). SUMO-modified target protein molecules are closely related to development and disease, especially tumor, metabolism, inflammation and immunity. SENPs play an important role in SUMO protein maturation and deSUMOylation. This review discussed the functions of SENPs in the development and progression of tumors and related mechanisms.

Objective To establish the quality standard for YI SHI MINGMU granules.Methods TLC method was used to identify Lycium barbarum L and Salvia miltiorrhiza.HPLC method was used to quantitatively analyze the concentration of Tanshinol sodium.The analysis was carried out on a column of Kromasil C18(4.6 mm×150 mm,5 μm)with a mobile phase of methanol and 0.5 % acetic acid at the flow rate of 0.4 ml/min.Column temperature was 30 ℃.The detection wavelength was 280 nm.Results The linearity range of Tanshinol sodium was 2.00~60.00 μg/ml ,r2=0.999 7(n=6),with the average recoveries of 105.62%, RSD=1.60%.Conclusion This method is accurate, simple, reliable and reproducible.It can be used for the quality control of YI SHI MINGMU granules.