Objective: To investigate the inhibitory effect of gypenosides on proliferation of human uterine cervical cancer HeLa cells in vitro, and to explore its possible mechanism. Methods: The HeLa cells were treated with different concentrations of gypenosides (low-dose group, 4.5 μg/mL; middle-dose group, 45 μg/mL; high-dose group, 450 μg/mL). The cells treated with 0.9% sodium chloride solution was designed as a control group. The inhibitory effect of gypenosides on the proliferation of HeLa cells was detected by MTT assay and BrdU (5-bromo-2-deoxyuridine) incorporation experiment. The effect of gypenosides on apoptosis of HeLa cells was detected by FCM (flow cytometry). The expression levels of Bcl-2, Bax, ERK1/2 (extracellular regulated protein kinase 1/2), p-ERK1/2 (phospho-ERK1/2), MEK1/2 (mitogen-activated protein kinase kinase) and p-MEK1/2 were detected by Western blotting. Results: The survival rate of HeLa cells was decreased significantly after treatment with gypenosides (45 μg/mL) at 24 h (P < 0.01). The survival rate of HeLa cells was decreased significantly after treatment with gypenosides (4.5 μg/mL) at 48 h (P < 0.01). The inhibitory effect of gypenosides (450 μg/mL) on the cell proliferation was confirmed by BrdU incorporation experiment. The apoptotic rate of HeLa cells was increased significantly after treatment with different concentrations of gypenosides. Gypenosides could down-regulate the expression levels of Bcl-2 and p-ERK1/2 and up-regulate the expression level of Bax. Conclusion: Gypenosides can significantly inhibit the proliferation of cervical cancer HeLa cells and also markedly induce the apoptosis. This effect may related to the up-regulation of Bax expression and down-regulation of Bcl-2 and p-ERK1/2 expressions. Copyright © 2013 by TUMOR.

Objective:To study the effects of transverse abdominis plane block (TAPB) combined with dexmedetomidine on the recovery quality after general anesthesia in cesarean section.Methods:From June 2019 to October 2020, 120 cases of cesarean section in Changsha Maternal and Child Health Hospital were randomly divided into dexmedetomidine group (Group D), transversus abdominis plane block group (group T), transversus abdominis plane block combined with dexmedetomidine (Group TD) and control group (Group C). Before anesthesia induction, local infiltration of 1% lidocaine was performed at the incision of all parturients. Propofol and rocuronium were used for induction under general anesthesia. After delivery of fetus, sufentanil and midazolam were injected intravenously, and propofol was pumped to maintain anesthesia. At the same time, dexmedetomidine was pumped into group D and TD, and normal saline was pumped into group T and C. After the posterior sheath of rectus abdominis was sutured in T group and TD group, two epidural anesthesia catheters were placed to the fascia of transverse abdominis on both sides, and 10 ml of 0.33% ropivacaine was injected respectively. The parturients were sent to postanesthesia care unit (PACU) after the operation. The time from the end of operation to the extubation of tracheal tube, heart rate (HR) and mean arterial pressure (MAP) before operation (T 1), after induction (T 2), after operation (T 3), at the time of extubation (T 4), 10 minutes after extubation (T 5) and at the time of leaving PACU (T 6) were recorded. Steward's wake-up score at T 4, T 5 and T 6, agitation during extubation (Sedation Agitation Scale, SAS score), PACU stay time and pain visual analogue scale (VAS) were recorded. Results:There was no difference in HR and MAP between the four groups at T 1, T 2, and T 3 ( P>0.05), while HR and MAP in group C at T 4, T 5 and T 6 were higher than those in the other three groups ( P<0.05). There was no significant difference in Steward′s wake-up score among the four groups ( P>0.05). The SAS score [(4.0±0.4)point], the time from the end of operation to extubation [(10.1±1.5)min] and stay time of PACU [(21±4.2)min] were the lowest in group TD than those in the other three groups ( P<0.05); the VAS score in group TD [(0.5±0.5)point] and group T [(1.1±0.4)point] were lower than that in group D [(4.1±0.3)point] and group C [(5.3±0.5)point] ( P<0.05). Conclusions:The application of general anesthesia combined with TAPB and dexmedetomidine in cesarean section may improve the quality of anesthesia recovery.

Objective To study the transduction dynamics, location of PTD-OD-HA fusion protein and its interaction with Bcr-Abl oncoprotein in K562 cell lines, and explore the influence of PTD-OD-HA fusion protein on oligomerization and tyrosine kinase activity of Bcr-Abl. Methods PTD-OD-HA fusion protein was labeled with FITC and co-cultured with K562 cells. The transduction efficiency of labeled PTD-OD-HA at different doses and time intervals was observed under fluorescence microscope. The location of labeled PTD-OD-HA fusion protein in K562 cells was detected by confocal microscopy. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was confirmed by coimmunoprecipitation. The phosphorylation of Bcr-Abl oncoprotein was detected by Western blotting. Results PTD-OD-HA fusion protein labeled with FITC was transduced into K562 cells in a dose- and time-dependent manner. PTD-OD-HA fusion protein was located in the cytoplasm of K562 cells and was consistent with the location of Bcr-Abl oncoprotein. The interaction of PTD-OD-HA fusion protein with Bcr-Abl oncoprotein was proved in K562 cells. This interaction could interrupt the homologous oligomerization of Bcr-Abl oncoprotein and reduce the phosphorylation of Bcr-Abl oncoprotein. Conclusion PTD-OD-HA fusion protein could be transduced into K562 cells efficiently, inhibit the oligomerization and reduce the phosphorylation of Bcr-Abl oncoprotein.

With the developments of endoscopic and interventional techniques, the role of surgical treatment in some diseases is gradually replaced by endoscopic or interventional treatment.The word surgery may not preserve only for surgeons, therefore it is redefined.According to different interventional pathways, digestive surgery can be divided into two categories, although both are with similar surgical essentials as resection and repair. Serosa surgery is traditional surgery,characterized for its pathway from skin to visceral serosa and cavity, either by open or laparoscopic platform. Mucosa surgery performs endoscopic operations from natural orifice then mucosa surface.The intercrossing or merging of different disciplines is an inevitable trend for modern medical science, traditional disciplines may be replaced by disease-centered medical model combined with multiple disciplines and techniques.Facing challenges, digestive surgeons should be confidential and inclusive;facing competitions, digestive surgeons should be brave enough for innovation and cooperation, and always regard patients' interest as his first priority.A universal norm for various disciplines should be set up on the practice of evidencebased medicine;surgeons in different specialties should learn from each other, and reinforcing comple-mentary advantages from each other;creating a novel disease-centered multidisciplinary heahhcare model on the basis of fusion of different disciplines is the development direction of digestive surgery.

Objective To study the effect of bisphenol A on the structure of the spleen and the expression of ER-? in F344 rats. Methods Fisher 344 rats were randomly divided into control,low-dose,moderate-dose and high-dose groups,10 in each and treated with bisphenol A through gavage at the doses of 4 mg/kg,40 mg/kg and 400 mg/kg respectively,once a day for 17 consecutive weeks. In the end of the experiment,the pathological examination of the spleen was done and the expression of ER-? was detected by western blotting. Results Compared with the control,the weight of high dose group decreased (P

[Objective]To investigate the prevalence rate of adolescent scoliosis in Heilongjiang province. [Methods]A total of 17 525 students(aged 7 ~15 years old) from 28 primary and secondary schools were screened for scoliosis from April 2007 to May 2009.Physical examination,corrugated photography and X-ray were used for the screening,prevalence rate was calculated and correspondent treatment was given to patients.[Results]The result of first check-positive was 911(5.20%),with male 448(4.92%),female 463(5.44%).The second check-positive was 413(2.36),with male 181(2.00%),female 232(2.73%),387 in them received the third check.In the third check,335 patients were found the Cobb's angle ≥10?,with the prevalence rate of 1.91%(male 147,1.63%,female 188,2.21%).[Conclusion]Adolescent scoliosis can be early found,early diagnosed,prevented and treated by general survey.

0.05). (4) The result of detoxincation in- vivo was not as good as that of antiserum.

Objective To investigate the changes of activation and apoptosis of T-lymphocyte in peripheral blood of patients with chronic obstructive pulmonary disease(COPD) at the attack and relieved stage and their clinical significance. Methods 64 cases of COPD were divided into 2 groups: the acute stage(n=34)and the relieved stage groups(n=30). The T-lymphocytes were marked by fluoromonoantibodies CD 3 +, CD 4 +, CD 25 + , CD 95 +, CD 3 +/CD 25 +, CD 8 +/CD 28 +,CD 8 +/CD 28 - and was counted by flow cytometry . Results Compared with the relieved stage, in patients with COPD at the acute attack period,the CD 3 +, CD 95 +, CD 3 +/ CD 25 +, CD 8 +/ CD 28 - were increased, CD 4 + ,CD 25 + had not obvious change, but CD 8 +/ CD 28 + was increased. Conclusions In COPD at the acute attack stage, the immune function was disordered as the result of the change of T subtype of lymphocytes and the reduction of total T lymphocyte.So except for treatment in accordance with their symptoms,immunodrug of cells should be used in order to raise the immune function of the patients.

Objective: To investigate the enhancement effect of trillin on anti-cancer function of fluorouracil in cervical cancer cells. Methods: HeLa cells were treated with trillin (300 |xg/mL), fluorouracil (10 μg/mL), and fluorouracil (10 μg/mL) plus trillin (30, 100 and 300 |xg/mL), respectively. The inhibitory effects of trillin and fluorouracil on proliferation of HeLa cells were assayed by MTT method. Flow cytometry was applied to test the apoptosis of HeLa cells treated with trillin and fluorouracil. The expression levels of mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinase (ERK), Akt and other proteins in apoptosis-related signaling pathways in Hela cells were determined by Western blotting. The xenograft tumor model of human HeLa cells in nude mice was established, and the anti-tumor effect of trillin in vivo was tested after intraperitoneal injection of trillin (300 mg/kg), fluorouracil (10 mg/kg), and fluorouracil (10 mg/kg) plus trillin (300 mg/kg), respectively. Results: As compared with treatment with fluorouracil alone, the combination of trillin and fluorouracil could improve the inhibitory effect on proliferation and apoptosis of HeLa cells (both P < 0.05). The tumor formation in nude mice administrated with trillin plus fluorouracil was inhibited, and this inhibitory effect was more significant than that in nude mice treated with fluorouracil alone (P < 0.05). The combination of trillin and fluorouracil inhibited the expressions of phosphorylated MEK/ERK and Akt proteins in HeLa cells, increased the activation of caspase-3 protein, down-regulated Bcl-2 expression, and up-regulated Bax expression. Conclusion: Trillin can enhance the anti-cancer function of fluorouracil on cervical cancer. It is suggested that trillin may be used as a potential chemosensitizer.