0.05) between the two groups.After the reperfusion,an absolute reduction of peak CK-MB can be observed in the atorvastatin group compared with the control group(253.24?35.21 U/L vs 361.30?26.31 U/L,P=0.040) whereas the SOD levels of the atorvastin group was higher than that in the control group(40.10?2.15 U/mL vs 36.05?6.97 U/mL,P=0.037).Conclusions Atorvastatin can reduce oxygen free radical produced during reperfusion,playing a protective role for the myocardial cell during I/R injury.

Objective To support a reasonable and standard TCM treatment principle for advanced non-small-cell lung cancer (NSCLC) by exploring TCM syndrome features in NSCLC patients with factor analysis. Methods 105 cases of advanced NSCLC patients,who were admitted to the hospital after 1st January,1998 from 18 to 75 years old,were studied retrospectively with factor analysis of the data. Results According to analysis and synthesis of tongue,pulse and symptoms,the deficiency of qi and yin (37.14%) and the stagnation of phlegm and blood stasis (39.05%) were the major syndromes when advanced NSCLC was diagnosed. Conclusion One of TCM intervention treatment principles for NSCLC,which includes invigorating qi and yin,promoting blood circulation and removing blood stasis,regulating the flow of qi and resolving phlegm,is proposed to instruct the application of herbs,Chinese traditional patent medicines or injections in integration treatment and stage treatment for improving quality of life and elongating survival period.

Objective To build a regression equation which can indicate the relationship between pH value of Chinese youth' arterial blood and geographical factors in China.Methods We collected pH value of young and geographical factors in China,and studied the relationship between them.Results We worked out a regression equation which could indicate the relationship between the pH value of Chinese youth' arterial blood and the geographical indicators.Conclusion If knowing the geographical indicators of each area in China,we can estimate the pH reference value of young people's arterial blood by using the equation.

Objective To analyze dialectical thinking and medication laws of TCM master LI Ji-ren in treating rheumatoid arthritis (RA); To provide references for clinical medication. Methods Medical cases of RA in outpatient department of the First Affiliated Hospital of Wannan Medical College during January 2014 to June 2015 treated by Professor LI Ji-ren. SPSS18.0 statistical software was used to carry out frequency analysis, correlation analysis, and cluster analysis. Results The included medical cases covered 123 cases, 199 prescriptions and 211 kinds of medicine. The total medication frequency was 3503, with 52 kinds of high frequency medicine (>10%), including Astragali Radix, Angelicae Sinensis Radix, Scorpio, Spatholobi Caulix, and Sargentodoxae Caulix. 36 kinds of medicine belonged to Liver Meridian of Foot-Jueyin (69.2%); 21 kinds of medicine belonged to Kidney Meridian of Foot-Shaoyin (40.4%); 20 kinds of medicine belonged to Spleen Meridian of Foot-Taiyin (38.5%); 16 kinds of medicine belonged to Heart Meridian of Hand-Shaoyin (30.8%). High frequency medicine was mainly to be sweet, spicy and bitter. High frequency medicine was mainly to be medicine with the functions of clearing heat, tonifying (tinifying qi, blood, yang and yin), activating blood and expelling stasis, and expelling rheumatism. 127 pairs of high frequency medicine was in positive correlation, among which 36 pairs showed the correlation coefficient >0.9. High frequency cluster analysis could be mainly divided into 6 categories. Conclusion The medication laws in this study and Professor LI Ji-ren's treatment for RA emphasize consolidating basis, and are in accordance with the ideas of expelling wind and humidity, activating blood and expelling stasis and stopping pain, which can provide references for clinic.

To examine the effect of Gankang Suppository on duck hepatitis B virus (DHBV), the serum biochemistry and hepatic histology in an animal model of DHBV infection, a model of DHBV infection was established by infecting 1-day-old Yingtaogu ducklings with DHBV-positive serum. The successful model was confirmed by PCR assay and 48 ducklings infected with DHBV were randomly divided into 3 groups: a Gankang Suppository treatment group, an acyclovir (ACV) group and a DHBV model group (control), with each group having 16 animals. All the animals were given the medicines for 4 weeks in a row. The serum of the animals was taken 14 and 28 days after the medication and 7 days after drug discontinuation. Real-time PCR was performed to detect the copy numbers of DHBV DNA in the serum. ALT and AST were dynamically monitored. The ducklings were sacrificed on the 7th day after the discontinuation of the treatment and livers were harvested and examined for inflammation and degeneration of liver cells by using HE staining. The results showed that on day 14, 28 after the treatment and day 7 after the withdrawal, the logarithmic values (log) of DHBV DNA copy numbers in ducklings of Gankang Suppository treatment group were significantly lower than that before the treatment (P=0.0092, P=0.0070, P=0.0080, respectively). Compared with DHBV model control group, the ALT level was significantly decreased (P=0.0020, P=0.0019, respectively) on day 28 after the treatment and on day 7 after the withdrawal. The AST level was also reduced on day 14 after the treatment (P=0.0298). Compared with the ACV control group, the level of ALT was lower on day 7 after the withdrawal (P=0.0016). Histologically, the hepatocyte swelling, vacuolous degeneration and acidophilic degeneration in Gankang Suppository treatment group were alleviated 7 days after the withdrawal as compared with model control group (P=0.0282, P=0.0084, P=0.0195, respectively). It is concluded that Gankang Suppository can effectively suppress DHBV replication, reduce the levels of serum ALT and AST and improve hepatic histology.

Objective To investigate the effect of cognitive behavior therapy combined with drug treatment of adolescent obsessive-compulsive disorder(OCD) and the effect on quality of life.Method Yale Brown Obsessive Compulsive Scale (Y-BOCS) and quality of life in children and adolescents scale (QLSCA) were used to assess the curative effect and quality of life for 123 adolescents with OCD on the psychological clinic in hospital.t test and one way-ANOVA test were used to analyze the data.Results The effective rate of drug treatment group was 64.52％ after 12 weeks and combined treatment group was 80.33％.The effective rate of drug treatment group was 77.42％ after 24 weeks and combined treatment group was 91.8％.There was significant difference between the two groups (P ＜ 0.05).Two groups before and after treatment had significant difference (P ＜ 0.01).There was significant difference between the two groups after 12 weeks (P＜ 0.01) and 24 weeks (P ＜ 0.05).After 24 weeks of treatment,two groups of patients in the overall evaluation of the quality of life and negative ernotion had significant differences (P ＜ 0.05) and in self satisfaction,peer relationship,learning ability and attitude had significant differences (P ＜ 0.01).Conclusion Combination therapy is better than simple drug treatment,and improves the quality of life better.

Objective To observe the change and its clinical significance of serum B-type brain natriuretic peptide (BNP) concentration in patients with acute cerebral hemorrhage. Methods The plasma BNP level was measured by microparticle enzyme immunosorbent assay (MEIA) method in 100 patients with acute cerebral hemorrhage. The blood volume of cerebral hemorrhage was computed respectively. The Glasgow Coma Scale (GCS) and National Institutes of Health Stroke Scale (NIHSS) were used to evaluate nerve function on the 1st, 3rd, 7th and 15th day after admission, and the Glasgow Out-come Scale (GOS) was used when the patient was discharged from hospital or died. Results The plasma BNP level at the 2nd day after admission was positively correlated with the blood volume of cerebral hemorrhage in patients with acute cerebral hemorrhage (r=0.367, P=0.000), with NIHSS score at the 1st, 3rd, 7th and 15th after admission (r=0.491, 0.444, 0.427 and 0.458, all P=0.000), and with GOS score (r=0.507, P=0.000). In addition, it was negatively correlated with GCS score at the 1st, 3rd, 7th and 15th after admission (r=-0.553, -0.429, -0.473 and -0.501, all P=0.000). Conclusions The plasma level of BNP is good for predicting the strike of cerebral hemorrhage patient′s condition, it is likely to provide a original thread for judge the severe degree of patient′s condition.

Objective To explore the levels and the significance of IL-11 and soluble glycoprotein 130(sgp130) in the treatment of new-diagnosed acute leukemia(AL) during the induced remission. Methods The levels of IL-11 and sgpl30 in 47 patients with acute leukemia were determined by ELISA respectively before treatment and after completed remission(CR), and the number of white blood cell (WBC) and platelet (Plt) were valued by hametometry. Results Plasma IL-11 level of AL patients was significantly lower than normal (P ＜0.05), and increased obviously to the normal level when complete remission was achieved. And it correlated with PLT counts. While sgp130 level was higher than normal (P＜0.05),and declined after CR and correlated with WBC counts. Conclusion The plasma IL-11 and sgp130 levels can be helpful for confirming the diagnosis,evaluating the efficiency and predicting the prognosis of AL.

Nuclear magnetic resonance (1H-NMR) fingerprint of Rhodiola rosea medicinal materials was established, and used to distinguish the quality of raw materials from different sources. Pulse sequence for water peak inhibition was employed to acquire 1H-NMR spectra with the temperature at 298 K and spectrometer frequency of 400.13 MHz. Through subsection integral method, the obtained NMR data was subjected to similarity analysis and principal component analysis (PCA). 10 batches raw materials of Rhodiola rosea from different origins were successfully distinguished by PCA. The statistical results indicated that rhodiola glucoside, butyl alcohol, maleic acid and alanine were the main differential ingredients. This method provides an auxiliary method of Chinese quality approach to evaluate the quality of Rhodiola crenulata without using natural reference substances.
Magnetic Resonance Spectroscopy ; methods ; Principal Component Analysis ; Rhizome ; chemistry ; Rhodiola ; chemistry

Objective To identify risk factors of lymph node metastasis in superficial esophageal squamous cell carcinoma (ESCC),and to provide evidence for treatment choice under endoscope.Methods From January 2007 to December 2011,285 patients with pathologically diagnosed ESCC who received surgery and had clear record of lymph nodes resection were enrolled.The clinical pathological data of these patients were analyzed,including age,gender,smoking and drinking history,history of cancer,family history of cancer,location,tumor size,presence of esophageal,depth of infiltration,differentiation,and vascular cancer embolus.Univariate analysis (chi square test or Fisher exact probability method) and multivariate Logistic regression analysis were performed for risk factors of lymph node metastasis assessment.According to the rates of lymph node metastasis,patients were divided into three groups as follows:low risk,high risk and extremely high risk of lymph node metastasis.KaplanMeier method was used to calculate the average survival time and cumulative five years survival rate.Results Among the 285 patients with ESCC,40 (14.0 ％) patients with lymph node metastasis.The results of univariate analysis showed that location (x2 =9.333),tumor length (Fisher exact probability method),depth of infiltration (x2 =9.327),differentiation degree (Fisher exact probability method) vascular cancer embolus (Fisher exact probability method) were significantly associated with lymph node metastasis (all P＜0.05).The results of multivariate analysis indicated that tumor length over 5 cm,invasion to submucosal layer and vascular cancer embolus were independent risk factors of lymph node metastasis,and the odd ratio was 17.408(95％ confidence interval (CI) 1.557 to 194.686),3.471(95％CI 1.440 to 8.365) and 6.256(95％CI 1.787 to 21.910),respectively.The lymph node metastasis rates of patients in low risk,high risk and extremely high risk group were 5.2％(6/115),15.8％ (24/152) and 10/18,respectively;the average survival times were (69.9 ± 2.4),(63.8 ± 2.1) and (51.7 ± 1.7) months,respectively.The cumulative five years survival rates were 59 ％,51 ％ and 31％,respectively,and the difference was statistically significant (x2 6.816,P=0.033).Conclusions The risk of lymph node metastasis is high in ESCC patients with tumor length over 5 cm,invasion to submucosal layer and vascular cancer embolus,and the prognosis is poor.Lymph node metastasis should be considered when endoscopic therapy is chosen.