Objective:To discuss the effects of BuShen capsule on sexual function of castrated rats model.Methods:The castration model animals set up by removing testicle were divided into 5 groups,the large,middle and small-dose BuShen capsule groups,Nanbao capsule group,saline group and blank control group.At the end of the experiment,change of the latency time of penis erection,the incubation period and the number of their seizing of the female rats and the coefficient of the penis,the foreskin gland,seminal vesicle,prostate,and levator ani muscle in rats were determined.Histological section was made and changes in form of organization were observed under the light microscope,the levels of serum testosterone and cortisol in the rats were measured by radioimmunoassay.Result:Large,middle and small-dose of BuShen capsule could shorten the penile erection latency of castrated rats model.Large and middle-dose could decurtate the latency of their seizing female rats,large-dose could increase the number of their seizing female rats,middle-dose could increase the number of their seizing female rats.Large and middle-dose could obviously raise the levels of serum cortisol,middle-dose could improve the coefficient of the levator ani muscle.Conclusion:BuShen capsule could encourage the secretions of prostatae and seminal vesicle,accrete the cavernous body of penis and promote the growth of levator ani muscle.BuShen capsule has the good effects of invigorating the kidney and strengthening yang.

Objective To study the effect of the ECV-304 cells modified with LIF gene on the ex vivo culture of HSC/HPC in cord blood.Methods The ECV-304 cells were infected by Eukaryotic Expression plasmid pcDNA3.0LIF,and the positive ECV-304 cells were obtained by selected with G418.These cells were used to co-culture with CD34+ cells of cord blood.The phenotype of CD34+ and CD34+ CD54+、CD34+ CD62L+ primitive progenitors was detected by flow cytometry.Results The LIF gene can express in ECV-304 cells steadily.ECV-304 cells modified with LIF gene can improve expansion of CB CD34+、CD34+CD54+ and CD34+ CD62L+ cells while sustaining the expression of homing-related adhesion molecule.Conclusion The ECV-304 cells modified with LIF gene can not only significantly expand CB hematopoietic progenitor cells ex vivo,but the expanded CD34+ cells may well retain their homing ability.

To investigate the repairing effect of fibrochondrocyte collagen construct in meniscus lesion, fibrochondrocytes were isolated and expanded in vitro , then seeded onto collagen construct .18 dogs with wedged shape lesion in the lateral meniscus were randomly divided into 3 groups: the lesion were filled with either cell collagen construct or collagen or untreated. The lateral meniscus was then examined grossly and histologically at different periods. The results showed that the lesions that had been filled with fibrochondrocyte collagen construct healed with fibrocartilage tissue. The results suggest that tissue engineering method is practical.

To investigate the behavior of meniscal fibrocartilage cells in vitro ,human meniscal fibrocartilage cells were harvested from adolescents and sequentially digestied with 0 50% trypsin and 0 2% collagenase (type2). The isolated cells were cultured in Ham'sF 12 medium and identified by histochemical methods. Cell proliferation, morphology and ultrastructure were observed by phase contrast microscope and electronic microscope. In primarily cultured cells, fusiform cells dominated and they were confirmed by the positive result of immunohistochemical examination for type collagen staining. Our results indicate that it is a simple and highly effective method.

The effects of cyclooxygenase 2 (COX 2) on alcohol induced liver injury in rats were investigated. Rats were randomly divided into three groups: Control group was given with corn oil plus dextrose; Model group with corn oil plus ethanol; Treatment group with corn oil plus ethanol and a selective COX 2 inhibitor celecoxib. All materials were injected into stomach through intragastric tubes. Liver samples were analyzed with histopathology, fatty staining and immunohistochemistry. Glutathione s transferase (GST) activity of liver tissue and plasma were detected. Levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum were measured. Compared with the control group, positive fatty staining cells and expression of COX 2 in liver, level of ALT and AST in serum and activity of GST in plasma were all significantly increased in the model group, but the GST activity in liver was decreased. However, these changes were all reversed in the treatment group. The results show that the model of alcohol induced liver injury in rats has been set up successfully, the selective COX 2 inhibitor celecoxib can protect against alcohol induced liver injury in rats and COX 2 may play a role in alcohol induced liver injury

s:To resolve the problem of the accuracy and standardization of STR PCR typing in forensic science practice,we have designed a new method to produce standard D12S375 allelic ladder.Seven different PCR amplified D12S375 allelic fragments were isolated from the gel,eluted into the distilled water and reamplified by PCR.The purified allelic fragments were then blunt end subcloned individually into the pUC plasmid vectors and transfected into competent E.coli DH5? TM cells.The sequencing results confirmed that the size and the constructure of the inserts were correct.The recombinant plasmids DNA with 7 inserts were then used as templates for reamplification to generate D12S375 standard ladder, with which the genetic polymorphisms of D12S375 locus in Chinese Han population in Chengdu,Hui population in Gansu and Wei population in Xinjiang were studied.

BACKGROUND:Intertrochanteric fractures can be generaly treated by surgical treatment. Along with deep research on the biomechanics of the proximal femur, proximal femoral locking compression plate appears recently. The locking plate fixation is not strong, can reduce the local stress shielding, and maintain optimal system stability, but fracture fixation failure often occurs due to the inappropriate choice of nail plate. OBJECTIVE:To evaluate the value of digital orthopedics technology in preoperative planning in locking plate fixation for intertrochanteric fracture. METHODS: Forty intertrochanteric fracture patients receiving CT tomography femur upper segment were selected and divided into two groups. In the conventional group, after reading X-ray films and CT images, patients received locking plate fixation. In the computer planning group, before repair, fracture model was established using Mimics software to segment fracture fragments, simulate operation reset and 3-matic software was used to reconstruct locking plate and screws. Locking plate was assembled with Mimics to obtain the best plate position, best screw angle and screw length. Proximal femoral locking compression plate fixation was performed. Fluoroscopy times, operation time, blood loss and fracture healing time were compared in both groups. RESULTS AND CONCLUSION: Three-dimensional models of proximal femur were reconstructed, and a series of data were obtained. The optimal position of each plate was obtained from each patient. The screw length was predicted, so preoperative operation planning was realized. Al patients were folowed up for 6-20 months. Fluoroscopy times, operation time, and blood loss were significantly less in the computer planning group than in the conventional group (P < 0.05). No significant difference in fracture healing time was detected between the two groups (P > 0.05). These findings suggest that digital orthopedics technology used in intertrochanteric fracture can simulate the locking plate position, determine the screw placement angle and length of the screw in advance, and reduce fluoroscopy times, operation time, blood loss and screw position misalignment.

According to the training of all levels and all kinds of personnel during our hospital's digital construction and implementation process,this article discussed the training char-acteristic of computer skill and the teaching principle for doctors and nurses

OBJECTIVE:To establish a method for determining plasma concentrations of tanshinone ⅡA and use it for pharma-cokinetics study of tanshinone ⅡA in Beagle dogs. METHODS:HPLC was performed on the column of Phenomenex Luna C18, with the mobile phase of water(5 mmol/L of ammonium acetate+0.05% H3PO4)-acetonitrile at flow rate of 1.0 ml/min;the detec-tion wavelength was 270 nm,column temperature was 40 ℃ and the volume was 20 μl. 9 Beagle dogs were randomly divided into tanshinone ⅡA low,medium and high dose groups(2,4 and 8 mg/kg),blood was respectively taken from forelimb venous blood to determine the plasma concentration before and after 2,5,10,15,20,30,45,60,75,90 and 120 min of iv administration. DAS 2.0 software was used to calculate the pharmacokinetic parameters. RESULTS:The linear range of tanshinoneⅡA was 0.097 5-12.50 μg/ml (r=0.999 8);the RSDs of precision and stability tests were all less than 10%;the method recovery was 100.4%-107.2%,and extraction recovery was 90.2%-92.3%. The t1/2αin tanshinoneⅡA low,medium and high dose groups were respective-ly(1.01±0.27),(1.03±0.46)and(1.51±0.65)min;t1/2β were respectively(16.25±4.78),(22.42±9.32)and(24.45±12.02) min;AUC0-120 min were respectively(150.88±45.25),(305.44±92.55)and(643.67±178.27)μg·min/ml;and CL were respective-ly(12.01±4.36),(12.78±5.06)and(12.17±5.41)ml/(min·kg). CONCLUSIONS:The precision,stability and recovery of the method are all in line with the determination requirements of biological samples,and tanshinone ⅡA showed a good linear relation-ship with dose in Beagle dogs in AUC0-120 min.

Objective To investigate the relationship between morning blood pressure surge (MBPS) and urinary albumin/creatinine (UmAlb/Cr) ratio in elderly patients with dipper essential hypertension.Methods Totally 273 elderly patients with dipper essential hypertension were divided into MBPS group (observation group,n =106) and non MBPS group (control group,n=167)according to the presence or absence of MBPS.The urinary UmAlb/Cr ratio,blood urea nitrogen (BUN),creatinine (Cr),blood glucose (Glu),high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C),triglyceride (TG) and cholesterol (TCH) were detected.The differences in UmAlb/Cr ratio between the two groups were analyzed.Results There were no statistical significances in sex ratio,age,course of hypertension,BUN,Cr,blood Glu,HDL-C,LDL-C,TG and TCH between the two groups (P＞0.05).The amplitude of variation in morning systolic pressure peak was higher in MBPS group than in non-MBPS group [(32.8±8.2) mmHg vs.(12.0±7.2) mm Hg,P＜0.001].UmAlb/Cr ratio was higher in MBPS group than in non-MBPS group [(4.93±2.17) vs.(4.23±2.42),P＜0.05].There were significant differences in UmAlb/Cr ratio between MBPS group and non-MBPS group in different genders [male:(4.95± 1.96) vs.(2.98 ±2.05),female:(5.89±2.24) vs.(5.03±2.37),both P＜0.05].Conclusions MBPS has a great influence on UmAlb/Cr ratio and tends to cause early impairment of renal function in elderly patients with dipper essential hypertension.