Heart fatty-acid-binding protein(H-FABP) is small molecular weight cytosolic protein that is abundant in the heart and has tissue specificity. Concentration of H-FABP changes regulation in serum after the myocardial damage. In addition it has a positive correlation with the degree of myocardial damage. It is an early diagnostic biochemical marker of acute myocardial ischemia. The advance of H-FABP in the research on physiochemical nature,and its application for myocardial injury and prospective study of sudden cardiac death in forensic science were reviews.

Objective:To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) in papillary thyroid carcinoma and their relationship to cervical lymph metastases.Method:In this study, the expressions of COX-2 and VEGF-C were examined by immunohistochemistry in papillary thyroid carcinoma tissues of 40 patients, and analysis was performed on the correlation of cervical lymph metastases with COX-2 and VEGF-C expression.Result:Positive expressions of COX-2 and VEGF-C were 70.0%(28/40)and 75.0%(30/40)respectively in papillary thyroid carcinoma. The positive rates of COX-2 and VEGF-C expression were 80.8%(21/26)and 84.6%(22/26)respectively in patients with cervical lymph metastases, and 50.0%(7/14)and 57.1%(8/14)respectively in patients without cervical lymph metastases, with a statistically significant difference between two groups(P<0.05, all). COX-2 was positively correlated to VEGF-C expression in papillary thyroid carcinoma(r=0.378, P<0.05).Conclusion:The results suggest that COX-2 and VEGF-C were highly expressed in papillary thyroid carcinoma, with possible interaction of their expressions, and may play a critical role in the cervical lymph metastases of papillary thyroid carcinoma.

Abstract Objective:To explore homology in mage-A family and find some common epitopes recognized by cytotoxic T lymphocyte(CTL) to oppose tumor escape.Methods:The amino acid sequence,differents open reading frame(ORF) and the known CTL epitope of themember of MAGE-A family,were analyzed using DNAstar software, and phylogenetic tree is also constructed. Results: MAGE-A family shareidentity nucleotide 57.6% - 98.0%,the phylogenetic tree showed that they are derived from a common ancestor at different time, as well asfound some CTL epitopes high similarity. Conclusion: MAGE-A family come from common an ancestor, but they bave many differences in thepattern of expression in tumor.This study can help to find the best CTL epitope vaccine to cure tumor.

Objective To investigate the secrection of Polytuftsin, a macrophage activator in Pichia pastoris expression system. Methods The recombinant plasmid encoding polytuftsin was transformed into the yeast by chemical method, and the positive clones were screened and expressed under the induction of methanod. It was observed that the influence of polytuftsin on macrophage in vivo and in vitro. Results It was verified that the recombinant products was confirmed to be our target protein by SDS-PAGE analysis and PCR assay. The expressed product could activate macrophage to secret cytokine and enhance the capacity of macrophages to kill L1210 tumor cells, the maximum killing rate is up to 40 %. In vivo the leukemia therapeutic trial, the time of tumor arise was delayed; the survival time of L1210 tumor-bear mice treated with Polytuftsin was extended, which is about 20 day. Conclusion The recombination protein polytuftsin, which could activate macrophage and has anti-tumor activity.

Objective To observe the effects of modified Yigong Powder (MYP) combined with chemotherapy on transplanted hepatocarcinoma of mice and to study its mechanisms. Methods Hepatocarcinoma 22 mouse model was established and then was used to observe the attenuating and synergic effects of MYP when applied together with 5-fluorouracil (5-FU). After 8 days of treatment,the tumor-inhibiting rate,activity of natural killer (NK)cells and interleukin-2 (IL-2) and small intestinal malondialdehyde (MDA) content were examined. Results MYP combined with 5-FU could increased the tumor-inhibiting rate to some extent and improve the immune function by increasing immune organ weight and increasing the activity of NK cells and IL-2. MYP combined with 5-FU could also reduce the 5-FU-induced intestinal injuries by relieving the damage of free radicals and inhibiting the lipid peroxidation and a good prognosis was expected in tumor-bearing animals treated with chemotherapy. Conclusion MYP exerts an attenuating and synergic effect when used together with 5-FU in treating tumor-bearing mice and its mechamism may be related to the improvement of immune function and reduction of intestinal injuries.

Objective To analyze the relationship of pathogenesis and early management with prognosis of sylvian fissure contusion of brain caused by traffic accident. Methods A review was done on 36 cases with sylvian fissure contusion of brain caused by traffic accident, in which early improvement of respiration and management of combined injuries were performed according to injury severity and pathogenesis. Standard big bone flap craniotomy was done in 31 cases including bilateral craniotomy in 13. Of nine cases treated conservatively, four cases turned to operation due to aggravation. Results Of all, 18 cases recovery better but death occurred in eight, vegetative state in two, bad disability in two and moderate disability in six. Conclusions Early synthetic treatment, prompt decompression with standard big bone flap, paying attention to sylvian fissure contusion in the midline area, dynamic observation of injury and effective treatment can improve prognosis and reduce mortality rate.

Objective:To explore the risk factors and event characteristics of unplanned removal of peripherally inserted central catheters (PICCs) in patients with chest tumors.Methods:The convenience sampling method was used to select chest cancer patients treated in a hospital in Shanghai between March 2018 and December 2021. Logistic regression analysis was used to identify the risk factors of unplanned removal in these patients.Results:A total of 242 patients were included in this study, of whom 42 (17.3%) experienced unplanned removal. The most common causes of unplanned removal in descending order were catheter related dermatitis, catheter slippage, catheter occlusion, catheter related thrombosis, and local or catheter related infections. Logistic multivariate regression analysis showed that a history of radiation therapy ( OR=0.295, 95% CI: 0.108 to 0.806, P=0.017) and a history of surgery ( OR=0.247, 95% CI: 0.076 to 0.799, P=0.020) were protective factors for preventing unplanned removal. Catheterization during spring or autumn ( OR=8.771, 95% CI: 2.828 to 27.204, P<0.001), catheter-related dermatitis ( OR=12.404, 95% CI: 4.176 to 36.845, P<0.001), catheter slippage ( OR=25.105, 95% CI: 7.040 to 89.523, P<0.001), occlusion ( OR=6.155, 95% CI: 1.713 to 22.111, P=0.005), and catheter related thrombosis ( OR=17.817, 95% CI: 2.848 to 111.479, P=0.002) were independent risk factors of unplanned removal. Unplanned removal may occur anytime when the patient had the catheter inserted, but is less likely to happen in the early stage and more likely in the first month after catheterization. Besides, catheter related complications demonstrated seasonality, with catheter related thrombosis and occlusion more frequent in autumn and winter, compared with dermatitis and catheter slippage showing no difference in incidence around the year. Conclusions:The incidence of unplanned removal is high in chest tumor patients with PICC. Nursing staff should familiarize themselves with the risk factors, event characteristics, and the seasonality of catheter related complications, and improve the evaluation and early intervention of complications, to reduce the incidence of unplanned removal and improve the patient's experience of living with catheters.

Objective To discuss the feasibility of estimation on intracranial pressure of patients withbrain injury by measuring optic nerve sheath diameter (ONSD) with uhrasonography.Methods From July 2008 to December 2011,90 patients with brain injury were selected.According to the admission Glasgow Coma Scale (GCS),they were divided into experimental group 1 (60 cases with light and medium brain injury,GCS 9-15 scores) and experimental group 2 (30 cases with severe brain injury,G CS 3-8 scores).The conventional physical examination 50 cases and volunteers 50 cases in neural surgical outpatient were selected as control group.ONSD of all groups were measured 3 mm behind the globe through orbital by ultrasonography with different time after admission.The intracranial pressure was measured at 0.5-1.0 h after ultrasonography by lumbar vertebra puncturing in different groups and analyzed statistically.Results After admission 1,3,7,14 d; ONSD in experimental group 1 respectively was (4.49 ± 0.31),(4.45 ±0.28),(4.41 ±0.32),(4A3 ±0.25) mm;ONSD in experimental group 2 respectively was (5.69 ±0.32),(6.30 ± 0.47),(5.71 ± 0.26),(4.77 ± 0.36) mm.After admission 1,3,7,14 d ;the intracranial pressure in experimental group 1 respectively was (78 ± 16),(83 ± 17),(90 ± 15),(82 ± 14) mmH2O (1 mmH2O =0.0098 kPa) ;the intracranial pressure in experimental group 2 respectively was (230 ± 22),(269 ± 21),(228 ± 13),(147 ± 22) mumH2O.ONSD and the intracranial pressure was (4.58 ± 0.41)mm and(88 ± 10) mmH2O in control group.ONSD and the intracranial in Experimental group 1 and control group had no difference (P ＞0.05); those of control group and experimental group 2,experimental group 1 and experimental group 2 had difference (P＜ 0.05).Conclusions ONSD and the intracranial pressure in light,medium brain injury patients have no change.In patients with severe brain injury after different time,the intracranial pressure change differently,ONSD enlargement with the intracranial pressure rising,examination of ONSD by ultrasonography can reflect the changes of the intracranial pressure,it is a new method to evaluate the intracranial pressure,has the certain application value.

Actins ; metabolism ; Adult ; Amputation ; Arthroplasty, Replacement, Knee ; Bone Neoplasms ; diagnosis ; metabolism ; pathology ; surgery ; Calmodulin-Binding Proteins ; metabolism ; Humans ; Leiomyosarcoma ; diagnosis ; metabolism ; pathology ; surgery ; Magnetic Resonance Imaging ; Male ; Radiography ; Tibia ; diagnostic imaging ; surgery

Animal models with kidney disease are generally divided into two types. One belongs to the models which imitate human kidney disease by the artificial operations, such as anti-glomerular basement membrane antibody nephritis, Heymann nephritis, anti-Thyl. 1 antibody nephritis, BSA nephritis and puromycin nephropathy. The other one pertains to the models which make themselves kidney disease, and appear the pathological characteristics naturally as like as human, such as HIGA mice with IgA nephropathy and NZB/WF1 and MRL/1pr mice with lupus nephritis. In addition,the transgenic animal models with kidney disease can also be established by the modern molecular biologic techniques including gene knockout and siRNA transfection. As for the studies related with kidney disease in pharmacodynamics and pharmacology of Chinese herbal medicine (CHM), it is important to understand deeply the features of each animal model with kidney disease, and select accurately the proper models according to the different experimental objectives, and then, build the special models provided with the combination of disease with syndrome in traditional Chinese medicine (TCM). Therefore,it is the developmental direction for the further study to establish animal models with kidney disease, which should possess the characteristics of syndrome in TCM.
Animals ; Diabetic Nephropathies ; etiology ; Disease Models, Animal ; Humans ; Kidney Diseases ; etiology ; Medicine, Chinese Traditional ; Mice ; Streptozocin