Objective: To study the chemical constituents in the roots of Goniothalamus cheliensis. Methods: Silica gel, ODS, and Sephadex LH-20 column chromatographic techniques were used to isolate and purify the chemical constituents and their structures were elucidated by spectral analyses. Results: Seven compounds were isolated and identified as acetylgoniofupyrone A (1), protocatechuic acid (2), pinocembrin (3), pinoresinol (4), goniodiol (5), 8-epi-goniotriol (6), and cardiobutanolide (7). Conclusion: Compound 1 is a new goniofupyrone-type of styryllactone, named acetylgoniofupyrone A.

OBJECTIVE:To observe analgesic efficacy of oxycodone acetaminophen after posterior lumbar fusion,and to opti-mize spinal postoperative analgesia plan. METHODS:In retrospective study,120 patients underwent posterior lumbar fusion were selected from the Affiliated People's Hospital of Hubei Medical College during Jun. 2013-Jun. 2014,and then divided into observa-tion group and control group according to therapy plan,with 60 cases in each group. Observation group was given oxycodone acet-aminophen 330 mg orally 6 h after surgery,and then 330 mg orally on 1st,2nd day after surgery,tid. Control group was given celecoxib 200 mg 6 h after surgery,and then 200 mg on 1st,2nd day after surgery,bid. Postoperative analgesic efficacy (VAS score) at resting state and active state,postoperative gastrointestinal motility recovery (the time to flatus) and the occurrence of ADR were observed in 2 groups after surgery. RESULTS:Whether at the resting state or the active state 24,48,72,120 h after surgery,VAS score of observation group was significantly lower than that of control group,with statistical significance(P<0.05). There was no statistical significance in the time to flatus and the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Oral administration of oxycodone acetaminophen possesses good effect to relieve spinal postoperative pain,showing fast and safe analgesic efficacy.

OBJECTIVE: To investigate the clinical features of primary biliary cirrhosis (PBC) and its treatment by integrated traditional Chinese and western medicine. METHODS: 16 PBC patients were observed. Ursodeoxycholic acid (UDCA) was used in the dose of 13 to 15 mg.kg(-1).d(-1), with some traditional Chinese herb prescription. Results (1) The proportion of women to men was 15:1, the mean age was 52.5 years. AMA-M2 was positive in 14 subjects (87.5%). Biliary tract enzymes and ESR were elevated in all subjects. The ratio of hypercholesterolemia (CHOL) and abnormality in IgM was high (62.5%). Fatigue, pruritus, arthralgia, jaundice, splenomegaly were noted in more than half cases. Every patient had one to four complications. (2) ALP, GGT and Glb declined evidently after 3 months' treatment by western medicine associated with traditional Chinese medicine, and declined markedly after 12 months' treatment (P<0.05 respectively). TBIL and CHOL declined gradually during the treatment course. Symptoms and signs were lightened. CONCLUSION: PBC has complicated and especial clinical features. UDCA therapy is effective in PBC, while traditional Chinese medicine has extraordinary effect in treating the symptoms and signs.

The property theory of Chinese herbal medicine (CHM) is regarded as the core and basic of Chinese medical theory, however, the underlying mechanism of the properties in CHMs remains unclear, which impedes a barrier for the modernization of Chinese herbal medicine. The properties of CHM are often categorized into cold and heat according to the theory of Chinese medicine, which are essential to guide the clinical application of CHMs. There is an urgent demand to build a cold/heat property classification model to facilitate the property theory of Chinese herbal medicine, as well as to clarify the controversial properties of some herbs. Based on previous studies on the cold/heat properties of CHM, in this paper, we described a novel strategy on building a cold/heat property classification model based on herbal bio-effect. The interdisciplinary cooperation of systems biology, pharmacological network, and pattern recognition technique might lighten the study on cold/heat property theory, provide a scientific model for determination the cold/heat property of herbal medicines, and a new strategy for expanding the Chinese herbal medicine resources as well.
Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Herbal Medicine ; Humans ; Phytotherapy ; Plants, Medicinal ; chemistry

Objective To explore the relationship between transforming growth factor-β1 (TGF-β1) and obesity-related glomerulopathy (ORG),and to analyze the possible mechanism for ORG and the new approach to its treatment.Methods Based on their body weight,30 SD rats were randomly divided into 2 groups : the normal control group (15 rats) fed with common food and the ORG model group (15 rats) fed with fat-enriched diets.The rats were sacrificed at the end of the 8th week,and their kidneys were taken out.Immunohistochemistry was used to detect TGF-β1 protein expression.Real time (RT)-polymerase chain reaction (PCR) was used to extract and detect the expression of TGF-β1 mRNA,and Western blot was applied to examine the expression of TGF-β1 protein.The findings were analyzed by using SPSS 13.0 software.Results Compared with the control group, qualitative TGF-β1 expression in ORG model group were significantly increased detected by immunohistochemistry mainly in renal tubules and interstitium.The average absorbance value of the control group and the model ORG group was 0.040-0.013,0.171 ± 0.084, respectively.The difference was statistically significant(P ＜ 0.05).The expression of TGF-β1 mRNA detected by RT-PCR was also increased compared with that of the control group(4.4 vs 0.6).The difference was statistically significant(P ＜ 0.05).The protein expression of TGF-β 1 examined by Western blot showed that it was more than that in the control group(4.3 vs 0.4).The difference between the control group and ORG model group was statistically significant(P =0.002).Conclusions The expression of TGF-β 1 in kidneys of ORG model rats increased, which not only indicates it can participate in ORG's occurrence and development, but also provide the basis to find out the mechanism and the approach to treatment.

Objectives:To evaluate the clinical significance of MMP-2,MMP-9 expression in gastrointestinal stromal tumor.Methods:54 of GIST were studied for MMP-2,MMP-9 by immunohistochemical staining,and the relationship of MMP-2,MMP-9 with each pathological factor were analyzed.Results:The positive expression of MMP-2,MMP-9 was 85.2%(46/54) ,83.3%(43/54) respectively.The expression of MMP-2,MMP-9 increased significantly according to the malignant increasing(P

Objective To investigate the effect of Atorvastatin on the proliferation and apoptosis of leukemic HL-60-cell line,and to explore the possible role of phosphatidylinositol 3-kinase/serine/threonine protein kinase /mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway in this process.Methods HL-60 cells were incubated with different concentrations of Atorvastatin (1,5,10 μmol/L),and HL-60 cells without any treatment were used as controls.The proliferation of HL-60 which was investigated by four methyl thiazolyl tetrazolium assay when cells were cultured for 12,24,48 hours.The apoptosis was detected by flow cytometry after cells were incubated for 48 hours.The mRNA and protein expressions of AKT,PI3K and mTOR were detected by reverse transcription-polymerase chain reaction and Western blot methods,respectively.Results The results indicated that Atorvastatin could inhibit the proliferation and induce the apoptosis of HL-60 cells.When treated with 10 μmol/L Atorvastatin after 48 h,the proliferation inhibition of HL-60 was observed most obviously,with a high rate of (39.77 ± 3.01) ％,compared with the control group,it had statistical significance (t =4.016,P ＜ 0.01),meanwhile,the apoptosis of HL-60 was most notable,at a rate of (43.29 ±3.91)％,compared with the control group,it had statistical significance (t =3.625,P ＜ 0.05).There were basal expression of AKT,PI3K and mTOR in the control group.When treated with 10 μmol/L Atorvastatin after 48 h,the mRNA expression of PI3K,AKT and mTOR were down-regulated most obviously,at a decrease of (37.05 ± 4.11) ％,(53.79 ± 3.27) ％,(40.63 ± 2.42) ％ (t =4.805,3.799,4.312,all P ＜ 0.05),respectively,in comparison with the control group.At the same condition,the protein expression of PI3K,AKT and mTOR were decreased most visibly,with a decline of (41.09 ± 3.17) ％,(45.67 ± 2.92) ％,(63.41 ± 3.59) ％ (t =3.576,4.727,4.902,all P ＜ 0.05) respectively in comparison with the control group.Conclusions Atorvastatin can inhibit the proliferation and induce the apoptosis of leukemic cell HL-60,and the mechanism may be associated with the PI3K/ AKT/mTOR signal pathway.

Objective To investigate the molecular mechanism underlying lymphocyte functionassociated antigen-1 (LFA-1) / intercellular adhesion molecule-1 (ICAM-1) mediated anti-neoplastic effects of cytokine induced killer (CIK) cells. Methods Lymphocytes isolated from peripheral blood of children leukemia were induced with interferon-gamma (IFN-y), anti-CD3 monoclonal antibody (CD3McAb) and interleukin-2 (IL-2) and co-cultured with dendrite cells (DC) to generate DC-CIK cells. When treated with LFA-1 monoclonal antibody, cytotoxicity of DC-CIK cells against leukemia cell lines was measured by the MTT assay, while RT-PCR and Western blotting were used to determine mRNA and protein expressions of GATA-3 and T-bet in DC-CIK cells, respectively. IL-12, IFN-γ and tumor necrosis factor-α (TNF-α) levels released by DC-CIK cells were quantified by ELISA. Results Induced DC-CIK cells were regular, round and transparent with variable cell volume and cellular aggregation. When treated with mouse anti-human LFA-1 monoclonal antibody, the cytotoxicity decreased mostly towards B95 cells under administration of 20 μg/ml LFA-1 monoclonal antibody in comparison with the control group(t =10.138, P ＜0.05). It led to a highest elevation of GATA-3 mRNA and protein levels (t =16.386, P ＜ 0.05; t =22.652, P ＜ 0.05) and a most decrease of T-bet mRNA and protein levels (t =17.728, P ＜0.05; t =17.452, P ＜0.05) under 20 μg/ml LFA-1 monoclonal antibody in B95 cells group in comparison with the control group. The expression levels of IL-12,IFN-γ, and TNF-o in supernatant were the lowest under 20 μg/ml LFA-1 monoclonal antibody in B95 cells group in comparison with the control group (t =21.621, P ＜0.05; t =13.739, P ＜0.05; t =15.278, P ＜0.05).Conclusion GATA-3 and T-bet were implicated in the LFA-1/ICAM-1 mediated anti-neoplastic effects of DC-CIK cells via activation of the Th1 pathway, with high secretion of Th1 cytokines, such as IL-12, IFN-γ and TNF-α.

Wheezing is the most common respiratory disease in children.In recent years,the incidence of childhood wheezing showed an upward trend,the hot topic in the current study is how to draw up a rational and effective treatment to reduce wheezing.This paper summarized the latest research progress of pathogenic factors,clinical classifi-cation,pathogenesis and prevention strategies in childhood wheezing.It aimed to provide a theoretical basis for early diagnosis and individual treatment for children with wheezing diseases.

Objective To investigate graduating nursing students′expected pressure, stress levels, nursing identification degree and their influence factors, provide a reference for hospitals and schools to enact measures to reduce pressure and improve nursing identification degree. Methods It was a cross-sectional survey. Three hundred and sixty- one graduating nursing students were selected and investigated by graduating nursing students′expected work pressure and expected resources before starting a career. Results Graduating nursing students′expected stress score was (2.13±0.53) points, nursing identification degree score was (2.57±0.57) points and they were both at median level and effected by several personal internal and external factors. Nursing students expected getting help from hospitals, schools and interpersonal aspects before starting a career. Conclusions Excepted stress and nursing identification degree are influenced by kinds of factors. Hospitals and schools should know about their expected requirements and enact measures to reduce pressure and improve nursing identification degree in time to keep nursing talent.