Objective: To investigate the chemo-sensitivity enhancing effect and its major active mechanism of Shengmai Injection (SMI). Methods: MTT assay was used to determine the chemo-sensitivity enhancing activity of SMI combined with various current- used chemotherapeutic drugs. Flow cytometry was conducted to determine the effect of Epirubicin combined with SMI on the cell cycle distribution and apoptotic cell death, and the mRNA expression level of MDR-1 gene was determined by RT-PCR. Results: SMI (30 μL/mL) combined with gemcitabine, cisplatin, paclitaxel, and Epirubicin exihibited the potent antiproliferative effects on human lung carcinoma A549, gastric carcinoma SGC-7901, breast carcinoma MCF-7, and hepatocellular carcinoma HepG-2 cell lines, respectively. SMI showed the most significant enhancing effects on HepG-2 cells in combination with Epirubicin, and the sensitivity of HepG-2 to Epirubicin was increased by 16 fold. Whereas SMI (30 μL/mL) alone did not affect the cell cycle distribution and apoptosis of HepG-2 cells, it enhanced the cellular responses when combined with Epirubincin together with a down-regulated mRNA level of MDR-1 gene. Conclusion: SMI combined with the different chemotherapeutic drugs could enhance the sensitivity of cancer cells significantly via down-regulating the mRNA expression level of MDR-1 and the expression of P-glucoprotein with the excretion of drugs.

Airway remodeling is the result of chronic inflammation, which including airway wall thickening, matrix and collagen deposition, epithelial hyperplasia and fibrosis, smooth proliferation and hypertrophy,fibroblast proliferation, and mucus glands and goblet cell proliferation, micrangium generation and other pathological changes. Airway smooth muscle change is known as the reason of airway hyper - responsiveness and asthma aggravating. There are many factors which can induce airway smooth muscle hypertrophy and proliferation, such as inflammation, cytokines,extracellular matrix and genetic factors. In addition, recent researches reveal the airway smooth muscle is also an important source of inflammation. In this paper the latest opinion of the role of asthma airway smooth muscle in the airway remodeling were elaborated,and inhale hormone earlier was suggested.

The damage of airway epithelial cell in asthma including epithelial cells differentiated into goblet cell, mucous cells metaplasia, which lead to the extracellular matrix increasing, airway walls fibrosis and mucus high secretion. There are many cytokines, growth factors, signal transduction pathways and gene regulating this process.Neuroendocrine cell plays a very important role in the immune adjustment.The repairing process of airway epithelial cells after damaged is a complicated process and affected by many factors.

The approach of photodynamic therapy combined with chemotherapy for tumor has received more and more attention. But its mechanism is not entirely clear, fortunately, with a lot of basic research and new photosensitizers continuing to emerge, so that the efficacy of this approach is enhanced increasingly. Photo-dynamic combined with chemotherapy in the treatment of hollow organ cancer has achieved good clinical results, but in real organ cancer treatment still requires further research.

Objective To summarize the treatment of perianal abscess with diabetes.Methods 90 patients with perianal abscess with diabetes were randomly divided into the triple therapy group,the microwave group,Chinese group 3 groups.Three groups received conventional surgery,control of blood sugar,anti-infection treatment.Microwave therapy group WaS given microwave therapy and Chinese medicine group was given Chinese medicine,triple therapy group increase chinese medicine treatment and microwave based conventional surgery.The effect,the average lost time of exudate,wound healing time was analyzed.Results The triple group was significantly improved efficiency,83.3%,total effective rate of 100%.Conclusion The triple therapy combined with conventional surgery,Chinese medicine and microwave treatment could promote postoperative wound healing and exudate disappeared time and improve the efficiency of treatment to relieve patient concerns of treating perianal abscess with diabetes.

Objective To deep understand and explore the real exerience and view of man nurses about nurse profession. Methods 8 nurses from 3 municipal hospitals were interviewed by semi-structured fo-cus interview. Then the data underwent analysis and finishing and the theme was concluded. Rusults The different feelings, conceptions,demands and suggestions from man nurses for nurse profession were got through the procedure of TrianguHation. Conclusions Nursing work needs the attending of man nurses, people should give extensive support on both spirit and government policy, change their concept in order to attract more men to join the nursing staff and adapt to nurse profession better.

Objective To explore the early characteristics,risk factors and correct diagnosis procedure of acute pulmonary embolism.Methods The onset,axillary examination and susceptibility of 52 cases of acute pulmonary embolism between Jan 2004 and Dec 2005 were analyzed.Results The early manifestation of acute pulmonary embolism was difficulty in breathing,chest pain,hemota and fatigue.Only 11.5%patients presented all the obove symptomes at the same time.D-dimer＞500μg/L was 100%.Blood gas analysis showed hypoximia in 94.2%,typical SⅠQⅢTⅢin 13.6% on electrocardiograph,pulmonary artery hypertension in 82.5%on ultrasonography,dilated right in 38.5%of all the subjects.The diagnosis rate was 82.7%at the first diagnosis.Lower limb deep vein embolism,chronic cardiac,kidney disease,malignant tumor and periooperation patients were the risk factors.Conclusion Acute pulmonary embolism is specific-free of clincial manifestation,patients with history of vertigo but excluding difficulty in breathing,chest pain,hemato should go proper examination.

As the first most common cause of death in China,stroke has become a public health problem that seriously affects national economy and people′s livelihood. Unfortunately,only 3% to 5% of stroke patients receive tissue plasminogen activator(tPA)treatment,the only pharmacological therapy ap?proved for ischemic stroke,and no drug is available for hemorrhagic stroke. Therefore,there is an ur?gent need to develop new drugs for stroke therapy. Despite the awareness that neuroprotective agents could be a common strategy for the treatment of both ischemic and hemorrhagic stroke,numerous neu?roprotective agents have showed failure in clinical trials. Combined with the current therapeutic strategies and drug development of stroke,this paper elaborated the stroke injury mechanisms and corresponding clinical drug research targeting excitotoxicity,oxidative and nitrosative stress,and inflammation. From a new perspective,this paper has proposed a novel therapeutic strategy targeting inherent defense mechanisms against stroke,with nicotinamide phosphoribosyltransferase(Nampt)- nicotinamide ade?nine dinucleotide defense system as an example to present our experimental evidence that Nampt can serve as an anti-stroke target and nicotinamide mononucleotide as an anti-stroke agent under development. It is hoped that the bottleneck of stroke therapy can be overcome with unremitting efforts so as to reduce the financial burden and mental stress,and bring benefits to people around the world.

Objective To assess the clinical efficacy of modified split patella in the treatment of patellar fracture.Methods 56 patients with patellar fracture were selected,and they were divided into two groups according to the random number method.The regular group was treated with common split patella,while the improved group was treated with modified split patella.In both two groups,the aspects of split patella slip,model large and knee pain were recorded.Results The incidence rates of model large and knee pain in the improved group were 3.57%,7.14%, which were 25.00%,32.14%in the regular group,the differences were statistically significant(χ2 =5.250,5.543,P=0.022,0.019).The incidence rate of split patella slip in the regular group was 3.57%,that was 0.00% in the improved group,the difference was statistically significant(χ2 =1.018,P=0.313).Conclusion Modified split patella mini-mizes the incidence of split patella model large and patellofemoral osteoarthritis.Thus,it deserves further application.

OBJECTIVE:To explore the characteristics and regularity of ADR induced by gynecological Chinese patent medi-cine,and to provide reference for rational use of gynecological Chinese patent medicine. METHODS:ADR reports were collected from gynecological ADR reporting information system of our hospital during 2008-2105;all ADR cases of Chinese patent medicine were collected and analyzed. RESULTS:No severe ADR was found among 41 cases of Chinese patent medicine-induced ADR,24 cases of new and common ADR(58.54%),17 cases of common ADR(41.46%);the incidence of ADR was in high level 1-3 d after medicating,being 97.6%. Main routes of administration were oral administration(19 cases,46.3%)and vaginal administra-tion(15 cases,36.6%)in ADR cases. Clinical manifestations were gastrointestinal reaction(29.97%),systemic reaction(19.15%) and mild injury of reproductive system(17.02%),etc. CONCLUSIONS:Chinese patent medicine is relatively safety in gynecologi-cal department. However,caution should be exercised in special cases and different medication.