Objective: To investigate the chemo-sensitivity enhancing effect and its major active mechanism of Shengmai Injection (SMI). Methods: MTT assay was used to determine the chemo-sensitivity enhancing activity of SMI combined with various current- used chemotherapeutic drugs. Flow cytometry was conducted to determine the effect of Epirubicin combined with SMI on the cell cycle distribution and apoptotic cell death, and the mRNA expression level of MDR-1 gene was determined by RT-PCR. Results: SMI (30 μL/mL) combined with gemcitabine, cisplatin, paclitaxel, and Epirubicin exihibited the potent antiproliferative effects on human lung carcinoma A549, gastric carcinoma SGC-7901, breast carcinoma MCF-7, and hepatocellular carcinoma HepG-2 cell lines, respectively. SMI showed the most significant enhancing effects on HepG-2 cells in combination with Epirubicin, and the sensitivity of HepG-2 to Epirubicin was increased by 16 fold. Whereas SMI (30 μL/mL) alone did not affect the cell cycle distribution and apoptosis of HepG-2 cells, it enhanced the cellular responses when combined with Epirubincin together with a down-regulated mRNA level of MDR-1 gene. Conclusion: SMI combined with the different chemotherapeutic drugs could enhance the sensitivity of cancer cells significantly via down-regulating the mRNA expression level of MDR-1 and the expression of P-glucoprotein with the excretion of drugs.

Intellectual disability is a genetically heterogeneous disease,however,more than half of the genetic etiologies still remain unexplained.Since whole exome sequencing is applied for intellectual disability,a mature work flow has been established,which firstly discovered some important causative genes.This paper reviews the application,advantages and disadvantages of whole exome sequencing for sporadic,unexplained intellectual disability.

Objective:To assess the efficiency of orthodontic management in the treatment of impacted tooth in dentigerous cyst.Methods:Nine cases of dentigerous cysts with impacted teeth were treated with fixed appliances, surgical exposure, scrape of cyst and orthodontic traction to assist eruption of impacted teeth.Results:After treatment the panoramic radiographs revealed that radiolucent lesion disappeared with full bone regeneration, and the lesion was replaced by normal bony density and structure. Roots of impacted teeth normally developed. Impacted teeth were orthodontically brought into their proper position with normal alignment, normal overjet and overbite. Normal occlusion was found in all the cases. Mean total active orthodontic treatment time was 21.25 months,varying from 14 to 36.Conclusion:Orthodontic management combined with surgical approach is effective in the treatment of impacted teeth in dentigerous cyst.

The account types,image construction,identification,information push frequency and contents of WeChat public account in 15 academic libraries of traditional Chinese medicine were analyzed by investigating the application of WeChat public account in these libraries, with suggestions put forward for further bringing the role of WeChat in-to play in information service of library, including clear position of WeChat public account, rational selection of its types,popularization and identification of WeChat public account ,enrichment of information push contents and expan-sion of personal service module.

Objective To explore the early characteristics,risk factors and correct diagnosis procedure of acute pulmonary embolism.Methods The onset,axillary examination and susceptibility of 52 cases of acute pulmonary embolism between Jan 2004 and Dec 2005 were analyzed.Results The early manifestation of acute pulmonary embolism was difficulty in breathing,chest pain,hemota and fatigue.Only 11.5%patients presented all the obove symptomes at the same time.D-dimer＞500μg/L was 100%.Blood gas analysis showed hypoximia in 94.2%,typical SⅠQⅢTⅢin 13.6% on electrocardiograph,pulmonary artery hypertension in 82.5%on ultrasonography,dilated right in 38.5%of all the subjects.The diagnosis rate was 82.7%at the first diagnosis.Lower limb deep vein embolism,chronic cardiac,kidney disease,malignant tumor and periooperation patients were the risk factors.Conclusion Acute pulmonary embolism is specific-free of clincial manifestation,patients with history of vertigo but excluding difficulty in breathing,chest pain,hemato should go proper examination.

Objective To investigate the relationship between chronic pharyngitis and lifestyle and obesity. Methods A total of 7 655 participants who underwent a health examination in the Navy General Hospital were recruited. These participants came from the same region and had similar occupations. Their lifestyle information data including smoking status, drinking status, and physical activity were collected. Differences of lifestyle and the prevalence of chronic pharyngitis were compared between men and women. Single-factor analysis and multivariate Logistic regression analysis were performed to identify the relationship of chronic pharyngitis and lifestyle and obesity. Results The proportion of smoking and drinking, the prevalence of chronic pharyngitis, levels of systolic blood pressure, diastolic blood pressure, triglycerides, and fasting blood glucose were significantly higher, and the ratio of lack of exercise and normal body weight significantly decreased, in men than those in women (P<0.01). There were no significant differences in age and LDL-C between different gender groups. The prevalence of chronic pharyngitis increased with the frequency of drinking and the increase of obesity in man and women. The prevalence of chronic pharyngitis was not statistically significant in man with smoking than that in man without smoking. There was no significant difference in the prevalence of chronic pharyngitis between females with or without smoking. The prevalence of chronic pharyngitis decreased with the increase of physical activity in both men and women (P<0.01). Higher levels of systolic blood pressure and fasting blood pressure and regular physical activity were protective factors, and obesity was a harmful factor of chronic pharyngitis in men and women. Currently, smoking and regular drinking were harmful factors of chronic pharyngitis in men but not in women. Old age and occasional alcohol consumption were risk factors for women. Conclusion There is a significant value of strengthening physical activity and maintaining normal body weight in the prevention and treatment of chronic pharyngitis. It is also important for men to quit smoking and limit drinking in the prevention and treatment of chronic pharyngitis.

OBJECTIVE To investigate the hospital infection monitoring and management in delivery room.METHODS A perfect management and surveillance system was established and carried out strictly.RESULTS We executed disinfection and isolation system strictly through rationalizing the layout of delivery room.The infection management work of delivery room marched into systematization and standardization.CONCLUSIONS Only when the delivery room infection management is strengthened can obstetrics quality be guaranteed to attain a designated standard.Safety of mother and infant can be ensured.The hospital′s social efficiency is increased.

Based on the theory of strategic human resources management,the author analyzes the present state of human resources in military nursing and its existing problems,and presents her thinking on the construction of a new strategic human resources management system in military nursing.

Nephrotic syndrome(NS) is a common disease in children.Glucocorticoids(GC) is the firstline treatment for NS and can cause growth hormone(GH) secretion and synthesis disorder through various ways which reduces levels of GH.Consequently,the growth and development are affected.For another,kddney disease can intervene in the secretion and regulation of insulin-like growth factor (IGF) and insulin-like growth factor binding protein(IGFBP).GH-IGF axis's change plays a role in children's growth retardation.Other factors,such as bone metabolic disturbance and hypothyroidism aggravates the growth inhibition.This review focuses on the prevention and treatment of growth retardation in children with NS.