BACKGROUND: Na+/dicarboxylate transporter protein (NaDC3) participates in the metabolism of energy, which may has effect on cellular senescence; however, the mechanism is not clear. OBJECTIVE: To investigate the role of human NaDC3 in cellular senescence by means of cell cycle and P16 measurements. DESIGN, TIME AND SETTING: The single sample observation was performed in the Nanjing Drum Tower Hospital from December 2007 to May 2008. MATERIALS: The WI-38 cell was purchased from ATCC Company, the retrovirus vector pLNCX2, sense vector PLNCX2-hNaDC3 and antisense vector PLNCX2-AshNaDC3 was provided by Clontech Company. METHODS: hNaDC3 was introduced into WI-38 cells with retroviral virus-mediated transfection. The integration and expression of exotic gene were confirmed. Whereafter, to examine the effects of hNaDC3 on cellular senescence of WI-38 cells by cell cycle and expression of P16. MAIN OUTCOME MEASURES: The integration and expression of hNaDC3, the cell morphology and generation of WI-38, the cell cycle and expression of P16 were observed. RESULTS: The results of Northern blot and Western blot revealed that hNaDC3 were expressed in WI-38/ hNaDC3 cells at gene and protein level, while, in WI-38/As hNaDC3 cells, there were integration of hNaDC3 mRNA with not protein expression. Compared with the control cells, WI-38 cells transfect with hNaDC3 cDNA showed cell generation decreased by eight to twelve passages, which in a low cell growth rate with cell cycle arrested at G1 phase, and the expression of P16 was elevated. CONCLUSION: Human NaDC3 gene may contribute to the process of cellular senescence of human diploid fibroblasts by increasing expression of P16.

Objective To discuss the effect of the bedside shielding protection on the first and second operators against position radiation in percutaneous coronary intervention.Methods The surface entrance dosage rate for the first and second operators at 125 cm height with and without radiation protection shielding (provided separately) were measured.The t-test was used for statistical analysis of arithmetic mean values of dosage rates.And the effect of bedside shielding protection was calculated.Results The position radiation dosage rate at which the first operator exposed was significantly higher than that for the second in the case of no bedside protection (t =97.1-2 263.0,P ＜ 0.05),whereas the dosage rate at which the first operator (except for the left foot position) exposed was significantly lower than that for the second in the case of no bedside protection (t =-80.9-275.1,P ＜ 0.05).The shielding effect of bedside protection on the first and second operators was in the range of 92.26％-99.36％ and 27.83％-97.90％,respectively.Conclusions The bedside protection may effectively reduce position radiation dose to patients and the use of bedside protection changed the trends in the dose distribution in operators' standing region.Attention should be focused on the radiation protection of the second operators in daily work by making full use of bedside protection.

Objective Spontaneous pneumothorax occurred mainly because of bulla rupture and its formation process and pathogenesis were unknown,the study was to detect the express level of the cytokines KL-6,FGF-10 and MMP-9 in the spontaneous pneumothorax patients with bulla and researched its significance.Methods Selected 24 cases of bulla resection for spontaneous pneumothorax patients,the immunohistochemical staining techniques and enzyme-linked immunosorbent assay (ELISA) was taken to detect the expression level of KL-6,FGF-10 and MMP-9 of the bulla site and the bulla adjacent site.Results Immunohistochemical results showed that the staining intensity of the KL-6 and FGF-10 in groups of bulla site was higher than those in groups of bulla adjacent site while there was no significant difference of MMP-9 in the two groups.ELISA results showed that the expression levels of the KL-6 and FGF-10 in groups of bulla site are higher than those in groups of bulla adjacent site and the results had statistically significant (P ＜ 0.05),while there was no statistically significant of MMP-9 in the two groups(P ＞0.05).Conclusion The expression of the KL-6 and FGF-10 in the bulla site in primary spontaneous pneumothorax patients was higher than that in the normal site ; the pulmonary fibrosis mediated by KL-6 and the lung-bronchial congenital abnormalities mediated by abnormal expression of FGF-10 might have correlation with bulla formation.There was no statistically significant of the MMP-9 expression between the two groups and the correlation between inflammation mediated by MMP-9 and bulla formation was not clear.

Objective To observe the clinical efficacy of acupuncture plus auricular point sticking in treating hemifacial spasm. Methods Fifty-six patients with hemifacial spasm were randomized into a treatment group and a control group, 28 in each group. The treatment group was intervened by acupuncture plus auricular point sticking, while the control group was by ordinary acupuncture, and the clinical efficacies were compared. Results The total effective rate was 89.3%in the treatment group versus 67.7% in the control group, and the difference was statistically significant (P<0.05). Conclusions Acupuncture plus auricular point sticking is an effective method in treating hemifacial spasm.

Objective To evaluate the diagnostic valu e of ~131I-MIBG adrenomedullary scintigraphy for high blood cat echolamine. Methods 96 cases of high blood catecholamine and 197 cases of other diseases were screened with ~131I-metaiodo benzylguanidine(MIBG) adrenomedullary scintigraphy. Results 56 cases of the total 60 cases of pheochromocytoma and 33 cases of the total 36 cases of adrenomedullary hyperplasia showed positive image. The positiv e rate were 93.3% and 91.7% respectively. 138 cases of primary hypertension, 4 9 cases of adrenal and other organic tumor, 4 cases of pheochromocytoma befor e operation, 10 cases of cured pheochromocytoma after resection and 3 cases of adrenomedullary hyperplasia all showed negative image. The total positive rate for high blood catecholamine was 92.7%, which was much higher than other method s, such as biochemical assay, type B ultrasound, computer tomography and magneti c resonance image. Conclusions ~131I-MIBG w as of great value for the locative and qualitative diagnosis of high blood catec holamine,especially for the silent, ectopic and multiple pheochromocytoma, the m etastatic malignant pheochromocytoma and adrenomedullary hyperplasia. It could be used for the differential diagnosis of high blood catecholamine from primary hypertension and other kinds of tumors.

Objective To investigate the protective effects of pretreatment with valsartan, an angiotensin Ⅱ type 1 receptor blocker, on the brain against ischemia-reperfusion (I/R) injury. Methods Thirty-six healthy male C57BL/6J mice aged 10-12 weeks weighing 20-25 g were randomly divided into 2 groups (n - 18 each): valsartan group (V) and control group (C). In group V valsartan 2 mg?kg-1 dissolved in 2.5% NaHCO3 100 ?l was given intraperitoneally (i.p. ) every day for 10 days before experiment while in group C 2.5% NaHCO3 100?l without valsartan was given. The animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1. Middle cerebral artery occlusion (MCAO) was produced by inserting an 8-0 nylon thread with rounded end into the left internal carotid artery and advancing it cranially until resistance was felt. MCAO was maintained for 1 h. The nylon thread was then withdrawn for reperfusion. A laser doppler blood flow detector (Omegaflo FLO-C1, Omegawave Co, Netherlands) was used to detect local cerebral blood flow (LCBF) at central and marginal infarct area [LCBF (%) = LCBF during I/R / baseline LCBF ? 100% ]. The model of MCAO was considered established when LCBF at central infarct area was 20% lower than the baseline value. LCBF was measured 10 min before MCAO (T0, baseline), as soon as MCA was occluded (T1) at 10, 30, 50 min of ischemia (T2-4) and at 10, 30, 60 min of reperfusion (T5-7) . MAP was measured immediately before valsartan administration, at T0 and T5. Neurological function deficit (NFD) was evaluated and scored (0 = no deficit, 4 = worst result) at 23 h after reperfusion was started . After evaluation of NFD the animals were anesthetized again and killed. The brains were removed. Cerebral water content was measured [cerebral water content (%) = (wet weight - dry weight) / wet weight ? 100%]. Infarct area was measured. Mortality rate was recorded.Results Pretreatment with valsartan did not affect MAP significantly but significantly reduced infarct area, brain water content, NFD and mortality rate and improved focal cerebral blood flow after MCAO. Conclusion Valsartan pretreatment can decrease cerebral infarct area induced by MCAO through improvement of focal cerebral blood flow after MCAO.

Objective To investigate the changes in pharmacokinetics of propofol and propofol concentration in the brain induced by normovolemic hemodilution ( NHD) . Methods Thirteen healthy male mongrel dogs weighing 10-15 kg were randomly divided into 2 groups: control group ( n = 7) and NHD group ( n = 6). The animals were anesthetized with intravenous diazepam 0.5 mg?kg-1 and ketamine 5 mg?kg-1 . The femoral artery and vein were cannulated. Lactated Ringer's solution was infused at 5-7 ml ? kg-1 ? h-1 . 30 min after induction of anesthesia NHD was conducted by removing blood from femoral artery and replacing it with 6% hydroxyethyl starch (HES) until Hct was reduced to 25% . Propofol was then infused at 10 mg?kg-1 ?h-1 for 30 min after NHD. Arterial blood samples were taken immediately before and at 1, 2, 5 , 10, 15, 20, 30, 31, 32, 35, 40, 50, 70, 90, 120, 150, 180, 240 and 300 min after propofol infusion was started for determination of plasma propofol concentrations. One week later the same NHD was repeated. Propofol was administered by TCI via Graseby 3500 infusion pump incorporated with Stanpump TCI software. Target plasma propofol concentration was set at 5 ?g?ml-1 . Arterial blood and CSF samples were obtained at 5, 15, 30, 45 and 60 min after the start of propofol infusion for determination of plasma propofol concentration ( bound and free) , plasma free propofol concentration and CSF propofol concentration. At the end of 60 min, after the collection of blood and CSF samples, brain tissue was obtained from the cerebral cortex of right frontal lobe for determination of brain propofol content. ECG, BP, arterial blood gases and body temperature were monitored during experiment.Results The plasma propofol concentrations were significantly lower during and after propofol infusion at 10 mg? kg-1 ? h-1 in NHD group than in control group ( P

The biological incompatibility of traditional peritoneal dialysis fluids(PDF) is the main reason for the poor outcomes of long-term peritoneal dialysis and the high rate of technical failure.Recently,two-compartment PDF has been applied in the treatment of peritoneal dialysis patients,and reportedly has lots of clinical advantages due to its better biocompatibility.This paper presents an overview of recent advances in the studies of two-compartment PDF.

Objective To understand the quality of malaria blood examinations in township?level hospitals,so as to provide the evidence for continuing the malaria blood examinations in the stage of post?malaria elimination. Methods A total of 64 township hospitals were investigated and 640 negative malaria blood slides were scored individually according to 10 indicators inMalaria Elimination Technical Schemein 2013 and 2014. The single and multiple indicators were calculated,and the work of blood examinations and situation of technicians were investigated. The data of malaria blood examinations and patient discovery in township hospitals of Nantong City were collected and analyzedduring the period of 2011-2014. Results Forthe single indi?cator,29.5%of the thick blood films did not reach the standard,and 35.8%of thin blood films did not reach the standard. For the multiple indicators,blood slides with more than 4 indicators below the standard(poor quality)accounted for 32.5%. From ma?laria blood examinations and malaria situation,the number of slides was 194 635 during the period of 2011-2014,and there were no local vivax malaria casesin 4consecutive years from 2011 to 2014,andlocal malaria has been effectively controlled in Nantong City. For health facilities where malaria patients initially presented,the township and village level accounted for 16.3%,and county and higher level accounted for 83.7%. Conclusions The quality of malaria blood examinations in township level hospitals of Nantong City is not high and the microscopic examination has a relatively low efficiency in the discovery of ma?laria cases. A new model for malaria blood examinations needs to be further explored.

Due to their small molecular weight,strong penetrating power,wide target range,strong ability of binding targets,stable quality,little immunogenicity,easiness to be synthesized and modified,and functional roles in molecular recognition and signal transduction,nucleic acid aptamers are now used as tools for molecular recognition and drugs delivery for the diagnosis and treatment of many diseases.