Object To study the suitable conditions on tissue culture of stem apex and shoot rapid propagation of ginger. Methods The ginger stem apex ((?)

[Objective] To guide the health preservation more scientifically and effectively through discussing relationship between natural timing and spirit-regulating, and studying the influence of lifecycle and time sequence regularity on the spirit-regulating. [Methods] The relationship of body and spirit, time property of the spirit, time sequence regularity and those lifecycle were adopted to analyse the theoretical basis and specific measures on regulating spirit based on time differentiation. [Results] Body as well as the spirit would be followed by the changes of the time sequence regularity, also at different stages of human body, the spirit changed with it accordingly. [Conclusion] TCM holds that body and spirit are unified, the lifecycle and natural timing affect human body and spirit correspondingly, so if we know the relation of the spirit and time, regulate spirit by time differentiation, which must be helpful for keeping good mind, even for health and longevity.

Acupuncture Therapy ; Humans ; Meridians ; Sensation

Objective To assess the characteristics of heart rate turbulencer (HRT) in elderly patients with decreased left ventricular diastolie function.Methods 40 patients were divided into two groups:20 patients with enlarged left atrium and 20 patients without enlarged left atrium.20 healthy people were selected as controls. Turbu1ence onset (TO) and turbulence slope (TS) were measured,and correlation was analyzed between TO,TS and the E/A,index of Macruz and heart rate variability (HRV).Results TO was higher (P<0.05) and TS was lower in patients (P<0.01),TO was higber in patients with enlarged left atrium than in with out enlarged lef atrium people (P<0.05) and TS was lower (P<0.01).TO was positively (P<0.01) and TS Was negatively (P<0.05),correlated with the index of Macruz.TO Was negatively (P<0.05) and TS was positively (P<0.05) related to SDNN and SDANN.Conclusion HRT can be used as a potential risk predictor in decreased left ventricular diastolic function patients.

Objective To verify the safety and efficacy of percutaneous coronary intervention of Type Ⅳ bifurcation lesion of left main by using a left main bifurcation strategy and crush stenting technique with domestic drug-eluting stents(DES).Methods The study population consisted of patients with isolated unprotected ostial stenosis of the left anterior descending(LAD)or circumflex(LCX)artery.Sequential steps of crush stent deployment and post-dilation were undertaken followed by a modified crush stenting technique with domestic DES.Clinical and angiographic follow up was obtained to assess the primary endpoint of death,non-fatal myocardial infarction(MI)or target lesion revascularization(TLR).Results Twenty-nine patients(21 males,8 females)with a mean age of 62.57?14.21 years were evaluated.All patients were successfully treated using crush stenting technique with which final kissing balloon inflations were performed.The radial approach was utilized in 44.8% of the procedures.The mean procedural time was 36.2?9.4 minutes while the mean fluoroscopic time was 18.3?3.5 minutes.LAD ostial lesion was found in 58.6% of the patients.Predilatation with balloon angioplasty was performed in 44.8% of the patients.Partner stents and Excel stents were used in 79.3% and 20.7% of the patients respectively.The average stent diameter was 3.76 mm and the average stent length was 18.19 mm of main branch.GP IIb/IIIa inhibitors were used in 6(20.7%)patients.Angiographic results from Quantitative coronary angiographic(QCA)data showed mean target lesion length was of 13.20?4.71 mm and the baseline ostial stenosis was 78.4%.Follow-up angiography at a mean interval of 11.5?2.7 months revealed late lumen loss of 0.06?0.10 mm and 0.21?0.12 mm in the main branch and in the side branch,respectively.Binary restenosis did not occur within the main branch and side branch stents.Clinical follow up was available in all patients with mean duration of 14.2?5.2 months.No cardiac death,non-fatal MI occurred and no TLR needed during the followup of all patients.Conclusion The application of modified crush stenting technique and final kissing balloon inflations with domestic DES may be a reasonable option for the treatment of Type Ⅳ bifurcation lesion of left main.

Objective:To detect the biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein.Methods:Human ?-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by objection with microscope and the method of MTT.The pure protein was proved by SDS-PAGE analysis.Results:The protein in the culture supernatant of the positive CHO cells transfected with ?-NGF gene could stimulate the growth of PC12 cell line and go into BBB.Conclusion:The target gene expressed successfully in the transfected CHO cell line and had good biological activity. [

Objective To explore the effective molecular mechanism of PPAR-γligands rosiglitazone to biliary ischemia-reperfusion injury in autologous liver transplantation. Method A total of 40 SD rats were randomly (random number) divided into sham operation group (SO), ischemia - reperfusion group (Ⅰ/R), rosiglitazone (ROS) and GW9662 group, with 10 ones in each. The models, rat biliary ischemiareperfusion injury of autologous liver transplantation, were made by modified two-cuff technique. Tissues of the liver and bile ducts and blood of those models were evaluated by pathological and biochemical methods to make sure the models were made successfully or not. SO group suffered autologous orthotopic liver transplantation, and L/R group suffered both that and ischemia-reperfusion. ROS group were injected rosiglitazone (0.3mg/kg) via portal vein after having been done all as I/R. GW9662 group suffered all as ROS, and 10min later ,they were injected GW9662(0.3mg/kg) via portal vein. 4h after the experiment, tissues of livers and bilary ducts were taken to be tested by immunohistochemistry method, and the blood punctured from the right ventricular were taken to be determined by ELISA. ANOVA was used for statistical analysis.Results IL-1β, TNF-α and IL-6 were mainly expressed in the cytoplasm of hepatocytes and bile duct cells,while NF-κB was expressed both in the cytoplasm and nuclei. Expression of those proteins in L/R and GW9662 group was increased, significantly higher when compared to the SO and ROS (P ＜ 0.05). IL-1β,TNF-α and IL-6 in rat serum were simultaneously increased, and significantly higher than SO(P ＜0.05).Compared with the SO, expressions of the IL-1 β,TNF-α and IL-6 were not significantly changed in ROS (P＞ 0.05 )but significantly increased in GW9662. Conclusions PPAR-γ ligand rosiglitazone took protective role in biliary ischemia-reperfusion injury in autologous liver transplantation. The mechanism correlates with the release of the IL-lα, IL-1β and TNF-α and other inflammatory mediators, which decreased as the expression of NF-κB inhibited by its antagonist.

As a new type of environmental pollutants, microplastics are widely distributed in the global ecosystem, and ingestion of microplastics may produce a number of toxic effects. Based on currently available publications, this paper describes the main pathways of exposure to microplastics, and summarizes the toxic mechanisms of microplastics in mammals, including oxidative stress, inflammatory response, immune damage, imbalance of gut microbiota, energy metabolism disorder and DNA damage, so as to provide insights into elucidation of the toxic mechanism mechanisms and health risk assessment of microplastics.

Objective To investigate the ischemic preconditioning time for inducing rat brain ischemia tolerance.Methods Procerebrum ischemic preconditioning injury was caused by occlusion of two-side common carotid artery for 10 minutes or 20 minutes,and subsequent cerebral ischemic injury was caused by occlusion of middle cerebral artery for 2 hours after 72 hours of reperfusion.The percentage of brain infarct size was calculated for the investigation of the proper ischemic preconditioning time for rat brain ischemia tolerance.Results Ischemic preconditionaing for 10 minutes and 20 minutes can reduce the percentage of brain infarct size significantly.The difference of the percentage of brain infarct size between 10-minute preconditioning group and 20-minute preconditioning group is insignificant,but the mean percentage of brain infarct size in 10-minute preconditioning group is less than that in 20-minute preconditioning group.Conclusion Ten minutes are the suitable time of ischemic preconditioning for rat brain ischemia tolerance.

Objective To study the hemoprotective effects of a rotary magnetic field(RMF)with radiation- injured mice.Methods C57 BL6/J mice were randomly divided into a control group and a magnetic treatment group.The mice received total body irradiation with 7.0 Gy and 6.5 Gy ~(137)Cs?rays.The treatment group was trea- ted with a RMF for one hour at a time,twice a day.The intensity of the RMF was 0.6T.The survival rate was ob- served for 30 days.On day 7,10,14,21,28 after irradiation,the subjects' peripheral blood cells were counted.On day 12 and 16,the number of bone marrow mononuelear cells(BMNCs)was measured and their ability to form granu- locyte-macruphage colony-forming unit(CFU-GM)was assessed.The pathological sectioning of the femur was per- formed and the expression level of bone morphogenetic proteins(BMPs)in the bone marrow were evaluated.Re- sults The RMF treatment increased the survival rate and duration among the irradiated mice and the number of blood cells in their peripheral blood.Also,RMF treatment could increase the number of BMNCs and improve their ability to form CFU-GM on days 12-16.Furthermore,RMF could improve angiogenesis and the expression level of BMPs. Conclusion The RMF treatment had an obvious protective effect against the effects of irradiation,and it accelerated the recovery of hematopoiesis and the hematopoietic microenviroment in mouse bone marrow.