2.Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages.
Ying HAN ; Yi-Xiang LI ; Cong JIN ; Chuan LI ; Jing QU ; Fu-Shun ZHANG ; Quan-Fu ZHANG ; Mi-Fang LIANG ; Pei-Hong QIU
Chinese Journal of Experimental and Clinical Virology 2013;27(3):161-163
OBJECTIVETo study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.
METHODSUsing two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.
RESULTSSFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.
CONCLUSIONThe results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
Bunyaviridae ; isolation & purification ; Cell Line, Tumor ; Endoplasmic Reticulum ; virology ; Fever ; virology ; Golgi Apparatus ; virology ; Humans ; Macrophages ; virology ; Thrombocytopenia ; virology
3.Application Status and Prospect of Bite Mark Evidence in Forensic Odontology.
Xin Fang MA ; Miao JIN ; Hao SUN ; Cong Bo MI
Journal of Forensic Medicine 2020;36(3):369-373
Bite marks are increasingly common in violent cases such as child abuse and sex crimes. Bite marks are often the result of a suspect's attack or a victim's self-defense. Because human teeth vary in size, shape and arrangement, bite marks on objects such as skin and food are characteristic. By using this principle, forensic odontology can identify or exclude suspects by comparing actual bite marks with the teeth marks in the mouth. In this paper, the practical application of bite mark evidence, the research status and problems of bite mark analysis are briefly reviewed, and the prospect of bite mark analysis is also discussed.
Bites, Human
;
Child
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Child Abuse
;
Face
;
Forensic Dentistry
;
Humans
;
Tooth
4.Sex Characteristics and Distribution of External Ear in Uygur Population of Xinjiang.
Xin Fang MA ; Tu Di MAIMAITI ; Jie Rui WANG ; Miao JIN ; Wu Si Man PATIGULI ; Cong Bo MI
Journal of Forensic Medicine 2020;36(5):642-647
Objective To analyze the characteristics of the bilateral external ears of Uygur adults by directly observing the morphological characteristics of the external ears of Uygur adults and classifying each feature. The frequency distribution of the characteristics was calculated to provide reference for forensic identification. Methods The 210 cases (75 males and 135 females) of bilateral external ear photos of Uygur adults in Xinjiang that met the inclusion criteria were collected. The frequencies of the features of the external ear were recorded and distinguished between the two sexes and the different sides. The data were statistically analyzed by SPSS 21.0 statistical software. Results The shapes of the external ears of males and females were commonly oblique or rectangular (34.67% of the left external ear of males and 41.33% of the right were oblique; 30.37% of the left and right external ear of females were rectangular), while triangular ears were the rare variants and the least common. Sex and bilateral differences were observed as regards the form of the helix in the subjects. Normally rolled helix was the most common (58.67% males and 61.48% females for the left ear; 60.00% males and 72.59% females for the right ear). Wide covering scapha helix was the most rare for the male left ear and flat helix was the most rare for the female right ear. Square and free earlobes were the most common (49.33% males and 62.96% females for the left ear; 40.00% males and 54.81% females for the right ear), whereas triangular earlobes were rarely seen. Single knob tragus (40.00% males and 37.78% females for the left ear; 37.33% males and 33.33% females for the right ear) and projection type of Darwin's tubercle (50.67% males and 40.00% females for the left ear; 48.00% males and 39.26% females for the right ear) were found to be common. Conclusion The characteristics of the bilateral external ears of male and female Uygur adults have differences, which can be used for forensic identification.
Adult
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Ear, External
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Ethnicity
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Female
;
Humans
;
Male
;
Sex Characteristics
5.Maternal Perfluorinated Compound Exposure and Risk of Early Pregnancy Loss: A Nested Case-control Study.
Xin MI ; Shi Qi LIN ; Xiao Fen ZHANG ; Jia Jia LI ; Li Jun PEI ; Feng JIN ; Qi LIAO ; Li Min XIE ; Li Cong WEI ; Chan Juan HAO ; Ya Wei ZHANG ; Wei LI
Biomedical and Environmental Sciences 2022;35(2):174-179