Methods: The experimental brain injury was induced in the SD rats by free falling object, and the expression of intercelluar adhesion molecule-1(ICAM-1)in the microvascular endothelial cells (EC) of rat brain was examined by immunohistochemistry and S-P histochemical methods.The rats were allocated to group A(normal blank control group), group B (administration of Borneolum), group C(brain injured model group) and group D(model rats treated with Borneolum).Results:ICAM-1 seldom expressed or never expressed in the normal rats brain,the administration of Borneolum could not induce its expression,and ICAM-1 expression increased in the injured brain(P

Objective To observe the effects of soybean isoflavone in attenuating trans fatty acids-induced athero-sclerosis in rats .Methods The rats were randomly divided into normal diet group、trans fatty acids group、soybean isoflavone group and trans fatty acids +soybean isoflavone group .Research kit was used to test the amounts of TG , HDL,LDL and IL-6,TNF-αin rats'blood.Bovine aortic endothelial cells were cultured and divided into control group,trans fatty acids group and trans fatty acids group +soybean isoflavone group .Western blot was performed to test the NF-κB phosphorylation ,as well as to determine ICAM-1 and VCAM-1 protein contents in bovine aortic endo-thelial cells.Results Compared with normal diet group ,the amounts of TG,LDL,IL-6,TNF-αincreased evidently (P<0.05), whereas the amounts of HDL decreased evidently (P<0.05) in the rats'blood of trans fatty acids group . After the soybean isoflavone intervened ,the amounts of TG,LDL,IL-6,TNF-αall decreased (P<0.01), meanwhile the amounts of HDL increased significantly (P<0.01).Soybean isoflavone inhibited NF-κB phosphorylation induced by trans fatty acids ( P<0.01) .The high expression of ICAM-1 and VCAM-1 induced by trans fatty acids was de-creased by soybean isoflavone when comparing with those in the control group ( P<0.01 ) .Conclusions Soybean isoflavone has a role in attenuating trans fatty acids trans fatty acids-induced atherosclerosis in rats .

This paper introduced multiple flexible teaching methods in medical immunology based on its characteristics including paying attention to introductory class,activating class atmosphere,integrating multiple teaching form.Results showed that these methods stimulating interests of the students,improving their comprehensive quality and ability of innovation,so teaching effect can be improved accordingly.

60 g in 15 cases).The operation time ranged from 35 to 85 minutes(mean,45),and the blood loss was 50-100 ml(mean,60 ml).No case needed blood transfusion.No rupture of the prostatic capsule,venous sinuses,or perforation of the bladder-prostate junction occurred in this series.The patients were followed up for 4 to 6 months,during which the Qmax increased to 16.4-23.2 ml/s(mean,19.8 ml/s),RU decreased to 0-15 ml(mean,5 ml),and IPSS score improved to 0-6.8(mean,4.2).No patient developed urinary incontinence.Conclusions Transurethral retrograde dissection of the prostate is effective for the treatment of BPH with a low morbidity rate of urinary incontinence.The procedure is simple and results in low blood loss.

Objective To investigate the effect of borneol administered by gastric gavage or external application on ICAM-1 expression in rat retina with ischemia-reperfusion injury.Methods Rat ischemia-reperfusion model was established.Laser microscope and biological analysis microsoft were used to observe the change of ICAM-1 expression in retina or rats after gastric gavage or external application of borneol.Results Borneol administered by gastric gavage or external application obviously decreased the expression of ICAM-1 after the modeling for 24 hours,the difference being significant as compared with that in the model group(P

Objective The increasing evidences indicate that the inhalable particle matter (PM10) is responsible for respiratory and cardiovascular morbidity and mortality.Generation of superoxide anion,hydroxyl radical and apoptosis in HLF cells induced by PM10 were studied in this paper to present some data for elucidating the mechanism of effects of PM10.Methods The superoxide anion and hydroxyl radical were detected respectively by cytochrome C assay and apoptosis inspected with Annexin V-FITC/PI double staining by flow cytometry.Results PM10 could generate the superoxide anion and hydroxyl radical,with significant dose-dependent manners,and the levels of the superoxide anion as well as hydroxyl radical were decreased by chitosan oligosaccharides (COS),with the significant protection effects (P

Objective To establish a three-dimensional finite element model of rapid canine tooth movement by reducing resistance and distracting procedures,and to discuss canine distal movement and force distribution of periodontium,and to provide theory basis for its clinical application.Methods Through 64-slice spiral scanning,temporomandibular joint (TMJ),the mandible,lower jaw denture section phantom DICOM data were obtained.Mimics software,Geomagic Studio 8.0 software,Unigraphics NX software,and Ansys11.0 software were mixed,and the three-dimensional finite element model were established under the conventional condition to move the canine (model 1 ),rapid canine movement through distracting osteggenesis of the periodontal ligament (model 2),rapid canine movement tooth movement through reducing resistance and distracting method (model 3),the strength were carried on three kind of models and the canine,the pericementum,the tooth socket bone stress distribution were observed.Results The biggest displacements on those three models occured in canine crown on 1/3,biggest displacement quantity:model 3 ＞model 2 ＞model 1,and the canine crown displacement reduced gradually from crown to the root point; in the model 1 most greatly equivalent stress mainly concentrated in the middle of distal alveolar crest,but in the models 2 and 3 most greatly equivalent stress centralism area shifted liguodistal alveolar crest.Conclusions In the process of canine distal movement,reducing bone resistance,can accelerate the tooth moving speed effectively and simultaneously,the canine has distal motion tendency.While under the action of force,the canine tends to move ligually with the canine distal movement,and thus appropriate measures should be taken to prevent canine lingual rotation in clinical work.

Phosphatidylinositol-3-kinase(PI3K),AKT,TSC1/2,and the mammalian target of rapamycin(mTOR) signaling cascade involves many cellular processes including apoptosis,growth,proliferation and differentiation,This pathway has been found to be the most variable one in human tumors.As tumor is a poorly differentiated disease,this review examines the role of the PI3K-AKT signal pathway in cell differentiation and tumor development.

Objective To explore the clinical,laboratory,and pathologic manifestations of the interstitial lung disease(ILD)associated with primary Sj(o)gren's syndrome(pSS,pSS-ILD).Methods Clinical data of 15 patients with pSS-ILD admitted to our hospital from 2006 to 2008 were collected and the different features were compared with 18 patients with pSS without ILD.Eight patients with pSS-ILD were followed up and observed the changes of high-resolution computed tomography (HRCT).Results The age at onset was later in pSS-ILD[(57±11)years]than in pSS without ILD[(43±11)years](P<0.01).The initial symptoms in six of patients with pSS-ILD were cough,expectoration,chest distress or dyspnea upon exertion.The respiratory clinical manifestations,circulationsystem involvement and pulmonary artery hypertension (PAH) were more common in pSS-ILD than in pSS without ILD(P<0.01).Compared with patients with pSS without ILD,patients with pSS-ILD had significantly higher serum IgG level(P<0.01).The common findings of HRCT included reticular changes,irregular linear hyper-attenuating areas,and nodules.Pleural involvement was found in 8 patients,honeycomb change in 5 patients and PAH in 3 patients.After treated for 6 months of 8 patients,radiological findings improved in 4 patients,stable in 2 patients,and worse in 2 patients.The comlnon histological findings included focal fibrosis in alveolar wall and alveolus with and alveolar space inflammatory cell infiltration,interstitial inflammation with mulifocal lymphocyte infiltration.One patient had the pathological changes of nonspecific interstitial pneumonia(NSIP).Conclusion The age at onset of pSS-ILD is late and tends to produce respiratory symptoms and prone to have circulationsystem involvement such as PAH and elevated serum IgG level.Honeycomb change in HRCT can be seen in one third of patients and most patients can improve after treatment,however.NSIP can be observed in histopathologieal

Objective To investigate the serum concentration of interleukin (IL)-17 in patients with active rheumatoid arthritis and the clinical implication. Methods Serum IL-17 level of 51 active RA patients were assessed by enzyme-linked immunosorbentassay (ELISA). The relationship between serum levels of IL-17 in active RA patients and clinic features were analyzed. Two independent samples t test, ANOVA for repeated, one-way ANOVA and Spearman's Chi-square test were used for statistical analysis. Results ① Serum IL-17 levels were elevated in active RA patients (365±63) pg/ml than in normal controls (86±13) pg/ml (P<0.01); ② Serum IL-17 levels in active RA was correlated with morning stiffiness duration, swelling index, Ritchie index, CRP, ESR, RF, X-ray score, DAS score (r =0.423, 0.213, 0.252, 0.276, 0.346, 0.251, 0.412, 0.398; P<0.05). ③ The levels of IL-17 between phase I (296±79) pg/ml and Phase II (360±89) pg/ml, phase II and phase HI (464±74) pg/ml, Phase I and Phase Ⅳ (369±83) pg/ml in RA group were statistically significantly different (P<0.05). Conclusion The levels of serum IL-17 in active RA patients are higher than the normal controls. The elevated serum IL-17 levels may be the useful markers for active RA.In addition,interleukin-17 is involved in disease progression and bony erosion of RA, and it maybe a new treatmenttarget of RA.