1.Insulin-Like Growth Factor-1 Increases the Expression of Inflammatory Biomarkers and Sebum Production in Cultured Sebocytes.
Hyojin KIM ; Sun Young MOON ; Mi Yeung SOHN ; Weon Ju LEE
Annals of Dermatology 2017;29(1):20-25
BACKGROUND: Acne vulgaris has been linked to the Western diet. Hyperglycemic diet increases insulin and insulin-like growth factor (IGF)-1. Deeper insights into IGF-1-mediated signal pathway are critical importance to understand the impact of Western diet. OBJECTIVE: We investigated the effect of IGF-1 on the expression of inflammatory biomarkers and sebum production in cultured sebocytes. METHODS: Polymerase chain reaction and enzyme-linked immunosorbent assay were performed to measure changes in the expression of inflammatory biomarkers including interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), IGF1R, IGFBP2, sterol response element-binding protein (SREBP), and phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PI3KCA) after the treatment of cultured sebocytes with 10⁻⁷ M or 10⁻⁵ M IGF-1. Sebum production was evaluated after the treatment of cultured sebocytes with 10⁻⁷ M or 10⁻⁵ M IGF-1 using lipid analysis. RESULTS: The expression levels of IL-1β, IL-6, IL-8, and TNF-α in cultured sebocytes after treatment with 10⁻⁷ M or 10⁻⁵ M IGF-1 were increased. Increased gene expression levels of NF-κB in cultured sebocytes were also shown after 10⁻⁷ M or 10⁻⁵ M IGF-1 treatments. Gene expression of these inflammatory biomarkers was decreased after 10⁻⁷ M or 10⁻⁵ M IGF-1 treatment in the presence of 100 nM NF-κB inhibitor. Treatment with 10⁻⁷ M or 10⁻⁵ M IGF-1 increased the gene expression levels of IGF1R, IGFBP2, SREBP and PI3KCA in cultured sebocytes. Sebum production from cultured sebocytes treated with 10⁻⁷ M or 10⁻⁵ M IGF-1 was also increased. CONCLUSION: It is suggestive that IGF-1 might be involved in the pathogenesis of acne by increasing both expression of inflammatory biomarkers and also sebum production in sebocytes.
Acne Vulgaris
;
B-Lymphocytes
;
Biomarkers*
;
Catalytic Domain
;
Diet
;
Diet, Western
;
Enzyme-Linked Immunosorbent Assay
;
Gene Expression
;
Insulin
;
Insulin-Like Growth Factor I
;
Interleukin-6
;
Interleukin-8
;
Interleukins
;
Polymerase Chain Reaction
;
Sebum*
;
Signal Transduction
;
Tumor Necrosis Factor-alpha
2.Expression of Protoporphyrin IX Induced by Liposome delta-5-aminolevulinic Acid in Cultured Sebocytes and Hair Organs.
Weon Ju LEE ; Seok Jong LEE ; Do Won KIM ; Kyung Duck PARK ; Mi Yeung SOHN ; Moon Kyu KIM ; Jung Chul KIM ; In Sook HAN
Korean Journal of Dermatology 2007;45(4):327-331
BACKGROUND: Photodynamic therapy with topical delta-5-aminolevulinic acid (ALA) has been introduced for effective treatment of facial acne. Topical routes for ALA have been explored via a photosensitizer delivery system. OBJECTIVE: We performed this study to evaluate absorption of liposome ALA (Lipo-ALA) into the sebocytes of cultured sebaceous glands and hair organs. METHODS: We cultured KGM sebocytes that were derived from human sebaceous glands. In addition, we cultured William E media pilosebaceous hair units that were isolated from the occipital scalp. Lipo-ALA (10 microgram/ml) and ALA (10 microgram/ml) were added to the 1 ml KGM containing sebocytes for 24 hours and the 1 ml William E media containing pilosebaceous hair units for 6, 24, 48 and 72 hours, respectively. RESULTS: Absorption of Lipo-ALA into the sebocytes of cultured sebaceous gland and hair organs was higher than that of ALA. CONCLUSION: Lipo-ALA may be considered as an effective photosensitizer in photodynamic therapy for facial acne.
Absorption
;
Acne Vulgaris
;
Hair*
;
Humans
;
Liposomes*
;
Photochemotherapy
;
Scalp
;
Sebaceous Glands
3.Effects of Magnesium Ascorbyl Phosphate on the Expression of Inflammatory Biomarkers after Treatment of Cultured Sebocytes with Propionibacterium acnes or Ultraviolet B Radiation.
Weon Ju LEE ; Sang Lim KIM ; Kyou Chae LEE ; Mi Yeung SOHN ; Yong Hyun JANG ; Seok Jong LEE ; Do Won KIM
Annals of Dermatology 2016;28(1):129-132
No abstract available.
Biomarkers*
;
Magnesium*
;
Propionibacterium acnes*
;
Propionibacterium*
4.Some Becker's Nevus Melanocytes Remain Alive after Treatment with Q-Switched Alexandrite Laser.
Han Jin JUNG ; Sun Young MOON ; Mi Yeung SOHN ; Yong Hyun JANG ; Seok Jong LEE ; Do Won KIM ; Weon Ju LEE
Annals of Dermatology 2017;29(3):352-355
No abstract available.
Lasers, Solid-State*
;
Melanocytes*
;
Nevus*
5.Effect of Vitamin D on the Expression of Inflammatory Biomarkers in Cultured Sebocytes Treated with Propionibacterium acnes or Ultraviolet B Irradiation.
Weon Ju LEE ; Min Ji KIM ; Hyo Sub RYU ; Mi Yeung SOHN ; Yong Hyun JANG ; Seok Jong LEE ; Do Won KIM
Annals of Dermatology 2016;28(5):665-669
No abstract available.
Biomarkers*
;
Propionibacterium acnes*
;
Propionibacterium*
;
Vitamin D*
;
Vitamins*
6.Effect of Vitamin D on the Expression of Inflammatory Biomarkers in Cultured Sebocytes Treated with Propionibacterium acnes or Ultraviolet B Irradiation.
Weon Ju LEE ; Min Ji KIM ; Hyo Sub RYU ; Mi Yeung SOHN ; Yong Hyun JANG ; Seok Jong LEE ; Do Won KIM
Annals of Dermatology 2016;28(5):665-669
No abstract available.
Biomarkers*
;
Propionibacterium acnes*
;
Propionibacterium*
;
Vitamin D*
;
Vitamins*
7.The Expression of Involucrin, Loricrin, and Filaggrin in Cultured Sebocytes.
Weon Ju LEE ; Kyung Hea PARK ; Hyun Wuk CHA ; Mi Yeung SOHN ; Kyung Duck PARK ; Seok Jong LEE ; Do Won KIM
Annals of Dermatology 2014;26(1):134-137
No abstract available.
8.Topical ALA-Photodynamic Therapy for Acne Can Induce Apoptosis of Sebocytes and Down-regulate Their TLR-2 and TLR-4 Expression.
Eugene JEONG ; Ji Won HONG ; Jung Ah MIN ; Dong Won LEE ; Mi Yeung SOHN ; Weon Ju LEE ; Jun Young LEE ; Young Min PARK
Annals of Dermatology 2011;23(1):23-32
BACKGROUND: Although photodynamic therapy (PDT) is widely performed for acne, little is known about its exact therapeutic mechanism. OBJECTIVE: We aimed to estimate the efficacy and safety of PDT on acne and to discover its mode of action. METHODS: We performed PDT on 12 patients with mild to moderate acne. The clinical efficacy was assessed by counting the acne lesions and measuring the sebum secretion before and after PDT. In addition, we took biopsy samples from the peri-lesional areas before and after 3-session of PDT. To examine the degree of apoptosis of the sebaceous follicles, TUNEL assay was performed. To investigate the changes of toll-like receptor (TLR)-2 and TLR-4 expression after PDT, immunohistochemical stainings were also carried out. Finally, we performed TUNEL assay using the cultured sebocytes to confirm the apoptosis of sebocytes in vitro after PDT. RESULTS: There was a significant reduction in the number of inflammatory acne lesions after PDT, compared to baseline (p<0.05). Sebum excretion significantly decreased 2 weeks after the first PDT session except for one patient (p<0.05). The TUNEL positive cells in the peri-lesional sebaceous glands after PDT markedly increased, compared with those of before PDT. A decrease in TLR-2 and TLR-4 expression by sebaceous glands and epidermis after PDT was 50% and 30%, respectively. CONCLUSION: Our results demonstrate that apoptosis of the sebaceous glands is associated with improvement of acne by PDT. PDT has shown to down-regulate TLR-2 and TLR-4 expression in the sebaceous glands and epidermis of acne patients.
Acne Vulgaris
;
Apoptosis
;
Biopsy
;
Epidermis
;
Humans
;
In Situ Nick-End Labeling
;
Photochemotherapy
;
Sebaceous Glands
;
Sebum
;
Toll-Like Receptors
;
Triazenes