Many of early findings regarding intestinal stem cells (ISCs) and their niche in the human intestine have relied on colorectal cancer cell lines and labor-intensive and time-consuming mouse models. However, these models cannot accurately recapitulate the physiologically relevant aspects of human ISCs. In this study, we demonstrate a reliable and robust culture method for 3D expanding intestinal spheroids (InSexp ) mainly comprising ISCs and progenitors, which can be derived from 3D human intestinal organoids (HIOs). We did functional chararcterization of InSexp derived from 3D HIOs, differentiated from human pluripotent stem cells, and optimization culture methods. Our results indicate that InSexp can be rapidly expanded and easily passaged, and show enhanced growth rates via WNT pathway activation. InSexp are capable of exponential cell expansion and cryopreservation. Furthermore, in vitro-matured HIO-derived InSexp proliferate faster than immature HIO-derived InSexp with preservation of the parental HIO characteristics. These findings may facilitate the development of scalable culture systems for the long-term maintenance of human ISCs and provide an alternative platform for studying ISC biology.

The distribution and morphology of cholecystokinin-8 (CCK 8)-, neuropeptide Y (NPY)-, Substance P (SP)- and vasoactive intestinal polypeptide (VIP)-immunoreactivity were examined in the cat superior colliculus (SC) by means of immunohistochemistry. Judging from this study, some neuropeptides-IR neurons may be present in the superficial layers of the sc. And these neurons and neuropeptides may be involved in the functions of the superficial layers.
Animals ; Brain* ; Cats* ; Immunohistochemistry ; Neurons* ; Neuropeptide Y ; Neuropeptides ; Substance P ; Superior Colliculi* ; Vasoactive Intestinal Peptide

Purpose: This study aimed to examine the mediating role of nursing professionalism in the relationship between critical thinking disposition and medication safety competency among peri-anesthesia nurses. Methods: Data were collected from 128 peri-anesthesia nurses with at least six months of experience in recovery-related departments across medical institutions nationwide. The analysis was conducted using IBM SPSS Statistics for Windows, version 24.0, including descriptive statistics, t-tests, one-way ANOVA, Scheffé tests, Pearson’s correlation coefficients, stepwise multiple regression, and the SPSS Process Macro. Results: Medication safety competency showed a significant positive correlation with critical thinking disposition (r=.79, p<.001) and nursing professionalism (r=.80, p<.001).Similarly, critical thinking disposition was positively correlated with nursing professionalism (r=.78, p<.001). Nursing professionalism was identified as a partial mediator in the relationship between critical thinking disposition and medication safety competency, with a 95% confidence interval of .11 to .63. Conclusion Critical thinking disposition significantly enhances medication safety competency, with nursing professionalism serving as a partial mediator.These findings underscore the importance of developing targeted educational programs to foster critical thinking skills and nursing professionalism, ultimately improving medication safety competency among peri-anesthesia nurses.

Purpose: This study aimed to examine the mediating role of nursing professionalism in the relationship between critical thinking disposition and medication safety competency among peri-anesthesia nurses. Methods: Data were collected from 128 peri-anesthesia nurses with at least six months of experience in recovery-related departments across medical institutions nationwide. The analysis was conducted using IBM SPSS Statistics for Windows, version 24.0, including descriptive statistics, t-tests, one-way ANOVA, Scheffé tests, Pearson’s correlation coefficients, stepwise multiple regression, and the SPSS Process Macro. Results: Medication safety competency showed a significant positive correlation with critical thinking disposition (r=.79, p<.001) and nursing professionalism (r=.80, p<.001).Similarly, critical thinking disposition was positively correlated with nursing professionalism (r=.78, p<.001). Nursing professionalism was identified as a partial mediator in the relationship between critical thinking disposition and medication safety competency, with a 95% confidence interval of .11 to .63. Conclusion Critical thinking disposition significantly enhances medication safety competency, with nursing professionalism serving as a partial mediator.These findings underscore the importance of developing targeted educational programs to foster critical thinking skills and nursing professionalism, ultimately improving medication safety competency among peri-anesthesia nurses.

Purpose: This study aimed to examine the mediating role of nursing professionalism in the relationship between critical thinking disposition and medication safety competency among peri-anesthesia nurses. Methods: Data were collected from 128 peri-anesthesia nurses with at least six months of experience in recovery-related departments across medical institutions nationwide. The analysis was conducted using IBM SPSS Statistics for Windows, version 24.0, including descriptive statistics, t-tests, one-way ANOVA, Scheffé tests, Pearson’s correlation coefficients, stepwise multiple regression, and the SPSS Process Macro. Results: Medication safety competency showed a significant positive correlation with critical thinking disposition (r=.79, p<.001) and nursing professionalism (r=.80, p<.001).Similarly, critical thinking disposition was positively correlated with nursing professionalism (r=.78, p<.001). Nursing professionalism was identified as a partial mediator in the relationship between critical thinking disposition and medication safety competency, with a 95% confidence interval of .11 to .63. Conclusion Critical thinking disposition significantly enhances medication safety competency, with nursing professionalism serving as a partial mediator.These findings underscore the importance of developing targeted educational programs to foster critical thinking skills and nursing professionalism, ultimately improving medication safety competency among peri-anesthesia nurses.

Purpose: This study aimed to examine the mediating role of nursing professionalism in the relationship between critical thinking disposition and medication safety competency among peri-anesthesia nurses. Methods: Data were collected from 128 peri-anesthesia nurses with at least six months of experience in recovery-related departments across medical institutions nationwide. The analysis was conducted using IBM SPSS Statistics for Windows, version 24.0, including descriptive statistics, t-tests, one-way ANOVA, Scheffé tests, Pearson’s correlation coefficients, stepwise multiple regression, and the SPSS Process Macro. Results: Medication safety competency showed a significant positive correlation with critical thinking disposition (r=.79, p<.001) and nursing professionalism (r=.80, p<.001).Similarly, critical thinking disposition was positively correlated with nursing professionalism (r=.78, p<.001). Nursing professionalism was identified as a partial mediator in the relationship between critical thinking disposition and medication safety competency, with a 95% confidence interval of .11 to .63. Conclusion Critical thinking disposition significantly enhances medication safety competency, with nursing professionalism serving as a partial mediator.These findings underscore the importance of developing targeted educational programs to foster critical thinking skills and nursing professionalism, ultimately improving medication safety competency among peri-anesthesia nurses.

No abstract available.

No abstract available.
Humans ; Infant*

SUMMARY: The present study was carried out to evaluate whether the coculture system of human embryos with Vero cells can improve the quality of embryo or overcome the repetitive implantation failures in order to obtain pregnancy. From January to December 1996, a total 202 cases which patients with the problems of repetitive implantation failures (group I) or those with the poor embryonic quality in their previous cycles (group II) was analysed. The quality of cocultured embryo, pregnancy, on-going and implantation rates between coculture and control groups were compared. Of 93 cases in group I, coculture was performed in 34 cases and conventional IVF for the rest. Of 109 cases in group II, 36 for coculture and 73 for conventional IVF. In group I, pregnancy, on-going and implantation rates in coculture group (14/34 (41.2%), 9/34 (26.5%), 16/81 (19.8%), respectively) were higher than those of control (11/59 (18.6%), 8/59 (13.6%), 12/152 (7.9%), respectively). There is significance in the pregnancy and implantation rates (p=0.028 and p=0.015). In group II, pregnancy, on-going and implantation rates in coculture group (8/36 (22.2%), 5/36 (13.9%), 8/87 (9.2%), respectively) were higher than those of control (5/73 (6.8%), 3/73 (4.1%), 3/158 (1.9%), respectively). Like the result of group 1, there is significance in the pregnancy and implantation rates (p=0.028 and p=0.022). Coculture system with Vero cells works well in the groups of the two indications. Although the case of 3 day-coculture was small as 15 cases in group II, 3 day-coculture improved pregnancy rate (4/15 (26.7%)). Therefore, 3 day-coculture with assisted hatching is recommended to the patients with poor embryonic quality. In conclusion, coculture system with Vero cells can be suggested as an effective method which improves pregnancy rate in those who have repetitive implantation failures or whose embryonic quality was poor in their previous cycles.
Coculture Techniques* ; Embryonic Structures ; Humans ; Humans* ; Pregnancy ; Pregnancy Rate ; Prognosis* ; Vero Cells

An increase in the dose of chemotherapy enhances the response of many experimental and clinical cancers, but the extent of chemotherapy dose escalation and repeated use is often limited by myelosuppression. The side effects of chemotherapy including bleeding and infection due to myelosuppression have resulted in delayed therapy and a reduction in the therapeutic dose, therefore it is necessary to overcome myelosuppression especially leukopenia in patients with gynecologic malignancies who recieved chemotherapy. This study is undertaken to investigate the clinical effects of rhG-CSF(recombinant human Granulocyte-colony stimulating factor) in 29 patients with gynecologic malignancy who recieved chemotherapy. It was given at a dose of 100 microgram bid/day subcutaneously until significantly increase of leukocyte count in leukopenic patient. The results showed, the rhG-CSF has significantly increased the number and function of leukocyte. The use of rhG-CSF was effective and useful to treat chemotherapy induced leukopenia and to accelerate the recovery from this complications.
Drug Therapy* ; Hemorrhage ; Humans* ; Leukocyte Count ; Leukocytes ; Leukopenia*