Thelper (Th) cells play a pivotal role in the regulation of immune responses. Since Th epitopes in adenovirus have not yet been defined, in this study, it was attempted to search for Th epitopes of adenovirus antigens that are restricted by MHC class II (H-2E). Among candidate viral proteins to be screened for Th epitopes, structural protein was selected, since they induced strong IL-2 release from adenovirus immune lyrnph node (LN) cells and the presence of E1 protein, which contains immunodominant cytotoxic T lymphocyte epitopes, did not potentiate the T cell responses. To confirm the presence of Th epitopes in the structural protein, virions were trypsinized and the resulting polypeptides whose molecular weights were lower than 5,000 were fractionated by HPLC. Some of the HPLC fraction turned out to induce LN cell proliferation. Ten synthetic peptides were designed as candidate Th epitopes from the primary amino acid sequences of adenovirus hexon and penton protein which are major constituents of the virion. The selected sequences share the common features of other known H-2E' binding ligands. Among these ten synthetic peptides, peptide of hexon protein amino acid residue 709-721 induced noticeable proliferation of LN cells from preimmune mice, and also able to induce IL-2 secretion from adenovirus-specific T hybridomas, suggesting that the peptide was the most immunodominant Th epitope. Hexon protein 221-233 and hexon protein 676-688 are considered as epitopes also. This study revealed three epitope sequences from adenovirus structural protein that are presented by class II MHC, H-2E.
Adenoviridae* ; Amino Acid Sequence ; Animals ; Cell Proliferation ; Chromatography, High Pressure Liquid ; Epitopes ; Epitopes, T-Lymphocyte* ; Hybridomas ; Interleukin-2 ; Ligands ; Mice ; Molecular Weight ; Peptides ; Trypsin ; Viral Proteins ; Virion

Recently, we experienced a patient with a B cell leukemia which could not be classified as chronic lymphocytic leukemia (CLL), prolymphocytic leukemia (PL) or follicular lymphoma in leukemic phase. He is a 58-year-old male and suffered from progressive lymphadenopathy at both inguinal and cervical areas. His blood film showed lymphocytosis (79%), and the cells were heterogeneous in size and shape. Twenty-five percent of the lymphocytes had nuclear irregularities or cleavages unlike the lymphocytes of CLL In which the cells often shows monomorphic features, small size with scanty cytoplasm and round nucleus. The bone marrow trephine biopsy specimen showed a diffuse pattern of infiltration of atypical Iymphoid cells. The lymph node histology showed atypical lymphoid colls proliferated as wide mantles around non-neoplastic appearing germinal centers. The immunophenotype of circulating lymphocytes in peripheral blood showed strong reactivity with CD5, CDl9 and CD20 without expression of CD2, CD3, CD7, CD10, CD22 and CD23. Although this case resembled CLL, the laboratory features showed major differences, notably in the Peripheral blood morphology, histology Patterns and the membrane Phenotype. By combining these data, we diagnosed this case as a leukemic phase of mantle cell lymphoma.
Biopsy ; Bone Marrow ; Cytoplasm ; Germinal Center ; Humans ; Leukemia, B-Cell ; Leukemia, Lymphocytic, Chronic, B-Cell ; Leukemia, Prolymphocytic ; Lymph Nodes ; Lymphatic Diseases ; Lymphocytes ; Lymphocytosis ; Lymphoma, Follicular ; Lymphoma, Mantle-Cell* ; Male ; Membranes ; Middle Aged ; Phenotype

Phylloides tumor is very similar to giant fibroadenoma in that they have benign appearance in breast radiologic image. Fibroadenoma has no malignant potential, but phylloides tumor is locally recurrent, invasive and may occasionally metastasize. It thus appears that evaluation of the differential point of the two tumor groups by radiologic study is very important. We retrospectively compared sonographic find;.ngs of 6 cases of phylloides tumor with those of 4 cases of fibroadenoma, which proved pathologically in Ye mgnam University Hospital from 1984 to 1986. The mean age of the patients were 31.8 years old(from 14 to 41 years old) in phylloides tumor and 28.8 years old (from 17 to 40 years old) in giant fibroadenoma, respectively. The viewpoints of this analysis were size, shape and contour of the masses, internal echo pattern, posterior enhancement, and especially the existence of peripheral cyst or septal band echo. We found that diffenentiation of these two tumors by sonography was difficult. But peripheral cyst was found only in phylloides tumor and septal band echo was found largely in giant fibroadenoma. Although the existence of peripheral cyst or septal band echo in the breast mass was not pathognomonic findings, we suggest that the existence of septal band echo is preferential finding to fibroadenoma, and peripheral cyst is preferential finding to phylloides tumor.
Breast ; Diagnosis, Differential* ; Fibroadenoma* ; Humans ; Retrospective Studies ; Ultrasonography

Various cancer types have been associated with cancer-related cerebral infarction. In this study, we describe the first case of cancer-related cerebral infarction in which the underlying disease was primary bone marrow lymphoma (PBML). A 79-year-old man presented with abruptly developed bilateral lower extremity weakness and confusion. Diffusion-weighted imaging on admission showed multiple cortical and subcortical embolic infarction lesions in multiple vascular territories. Diagnostic evaluations to determine the embolic source revealed no abnormalities. Laboratory testing demonstrated elevated D-dimer (2.59 μg/mL) but no other prothrombotic abnormalities. In suspicion of cancer-related stroke, we performed chest CT, abdomen CT, and FDG-PET to detect the hidden malignancy. Findings revealed no evidence of cancer; however, they did reveal signs of anemia (hemoglobin 9.0 g/dL). Bone marrow aspiration biopsy showed large atypical B cell involvement suggestive of high-grade B cell lymphoma. The patient was diagnosed with primary bone marrow diffuse large B-cell lymphoma initially presenting with ischemic stroke. Our case suggests that primary bone marrow cancer may be a candidate for the differential diagnosis of hidden malignancy in patients with suspected cancer-related stroke. Bone marrow biopsy may be essential for establishing an appropriate differential diagnosis in patients with abnormal hematologic findings.

OBJECTIVES: This study aimed to identify the process of psychosocial assessment, the experience of intervention, and the improvement after the disaster that Danwon high school students survived from the sinking of the Motor Vessel (MV) Sewol in South Korea on April 16, 2014. METHODS: We conducted in-depth qualitative research using individual interviews from January to February 2017. Twenty-one of 75 Danwon high school students survived by the MV Sewol disaster were studied. Two interviewers participated in the in-depth interview. Interviews were transcribed verbatim, coded, and analyzed through content analysis based on psychosocial assessment and intervention after the disaster. RESULTS: Twenty-one participants were 20 years old, where 10 were male (47.62%), and 11 were female (52.38%). More than 75% of the interviewed students felt that mental health services were needed, and more than 85% required mental health assessments. Regarding psychiatric symptoms, the students reported that they suffered depression and insomnia (19.05%), anxiety (14.29%), nightmares and phobias (9.52%), and difficulties regarding concentration, aggression, and game addiction (4.76%). CONCLUSION: Despite survivors experiencing the same disaster, there were differences in their responses to mental health assessments and interventions experienced during the three years, and conflicting opinions were reported. In planning future evaluations and interventions, it is necessary for strategies to cope with flexibly to consider the characteristics and symptoms of the survivors while maintaining principles.
Aggression ; Anxiety ; Depression ; Disasters* ; Dreams ; Female ; Humans ; Korea ; Male ; Mental Health Services ; Mental Health* ; Phobic Disorders ; Qualitative Research ; Sleep Initiation and Maintenance Disorders ; Survivors*

Purpose: This study aims to clarify the concept of mindful eating in healthcare and provide a basis for the development of related interventions in the healthcare field. Methods: This study was conducted according to the framework based on Rodgers' evolutionary view. The initial data of 879 in total were collected through databases (PubMed, CINAHL). included article only written in English and search term was ‘mindful’ and ‘eating’, and ‘health or healthcare or health science or medical or nursing'. There was no restriction on the year of publication and disciplines. In a total of 879 articles, 81 were finally included according to the researcher's selection and exclusion criteria. Results: A total of 4 attributes of mindful eating were identified ('Concentration to food', 'Sustained attention to self', 'Self-regulation', and 'The pursuit of pleasure'). There were 2 Antecedents ('Response to craving' and 'Voluntary will') and 3 Consequences ('Changes in eating habits', 'Physical health', and 'Emotional health') for this. Conclusion This concept analysis describes mindful eating within the context of healthcare. It is anticipated that this paper will enhance the clarity and precision of its use in future tool development and intervention research, while promoting its broader application in the field of nursing.

Purpose: This study aims to clarify the concept of mindful eating in healthcare and provide a basis for the development of related interventions in the healthcare field. Methods: This study was conducted according to the framework based on Rodgers' evolutionary view. The initial data of 879 in total were collected through databases (PubMed, CINAHL). included article only written in English and search term was ‘mindful’ and ‘eating’, and ‘health or healthcare or health science or medical or nursing'. There was no restriction on the year of publication and disciplines. In a total of 879 articles, 81 were finally included according to the researcher's selection and exclusion criteria. Results: A total of 4 attributes of mindful eating were identified ('Concentration to food', 'Sustained attention to self', 'Self-regulation', and 'The pursuit of pleasure'). There were 2 Antecedents ('Response to craving' and 'Voluntary will') and 3 Consequences ('Changes in eating habits', 'Physical health', and 'Emotional health') for this. Conclusion This concept analysis describes mindful eating within the context of healthcare. It is anticipated that this paper will enhance the clarity and precision of its use in future tool development and intervention research, while promoting its broader application in the field of nursing.

Purpose: This study aims to clarify the concept of mindful eating in healthcare and provide a basis for the development of related interventions in the healthcare field. Methods: This study was conducted according to the framework based on Rodgers' evolutionary view. The initial data of 879 in total were collected through databases (PubMed, CINAHL). included article only written in English and search term was ‘mindful’ and ‘eating’, and ‘health or healthcare or health science or medical or nursing'. There was no restriction on the year of publication and disciplines. In a total of 879 articles, 81 were finally included according to the researcher's selection and exclusion criteria. Results: A total of 4 attributes of mindful eating were identified ('Concentration to food', 'Sustained attention to self', 'Self-regulation', and 'The pursuit of pleasure'). There were 2 Antecedents ('Response to craving' and 'Voluntary will') and 3 Consequences ('Changes in eating habits', 'Physical health', and 'Emotional health') for this. Conclusion This concept analysis describes mindful eating within the context of healthcare. It is anticipated that this paper will enhance the clarity and precision of its use in future tool development and intervention research, while promoting its broader application in the field of nursing.

Purpose: This study aims to clarify the concept of mindful eating in healthcare and provide a basis for the development of related interventions in the healthcare field. Methods: This study was conducted according to the framework based on Rodgers' evolutionary view. The initial data of 879 in total were collected through databases (PubMed, CINAHL). included article only written in English and search term was ‘mindful’ and ‘eating’, and ‘health or healthcare or health science or medical or nursing'. There was no restriction on the year of publication and disciplines. In a total of 879 articles, 81 were finally included according to the researcher's selection and exclusion criteria. Results: A total of 4 attributes of mindful eating were identified ('Concentration to food', 'Sustained attention to self', 'Self-regulation', and 'The pursuit of pleasure'). There were 2 Antecedents ('Response to craving' and 'Voluntary will') and 3 Consequences ('Changes in eating habits', 'Physical health', and 'Emotional health') for this. Conclusion This concept analysis describes mindful eating within the context of healthcare. It is anticipated that this paper will enhance the clarity and precision of its use in future tool development and intervention research, while promoting its broader application in the field of nursing.

BACKGROUND: The bacterium Chlamydia trachomatis is one of the leading causes of sexually transmitted diseases worldwide. Since no simple and effective tool exists to diagnose C. trachomatis infections, we evaluated a novel point-of-care (POC) test, aQcare Chlamydia TRF kit, which uses europium-chelated nanoparticles and a time-resolved fluorescence reader. METHODS: The test performance was evaluated by comparing the results obtained using the novel POC testing kit with those obtained using a nucleic acid amplification test (NAAT), using 114 NAAT-positive and 327 NAAT-negative samples. RESULTS: The cut-off value of the novel test was 20.8 with a detection limit of 0.27 ng/mL. No interference or cross-reactivity was observed. Diagnostic accuracy showed an overall sensitivity of 93.0% (106/114), specificity of 96.3% (315/327), positive predictive value (PPV) of 89.8% (106/118), and negative predictive value (NPV) of 97.5% (315/323). The sensitivity of the novel test was much higher than that of currently available POC tests. Furthermore, the relative ease and short turnaround time (30 min) of this assay enables C. trachomatis-infected individuals to be treated without a diagnostic delay. CONCLUSIONS: This simple and novel test is a potential tool to screen a larger population, especially those in areas with limited resources.
Adult ; Aged ; Aged, 80 and over ; Chlamydia Infections/*diagnosis ; Chlamydia trachomatis/*genetics/isolation & purification ; DNA, Bacterial/chemistry/metabolism ; Europium/*chemistry ; Female ; Humans ; Male ; Metal Nanoparticles/*chemistry ; Middle Aged ; Point-of-Care Systems ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction ; Sensitivity and Specificity ; Young Adult