Objective To observe the effects of soybean isoflavone in attenuating trans fatty acids-induced athero-sclerosis in rats .Methods The rats were randomly divided into normal diet group、trans fatty acids group、soybean isoflavone group and trans fatty acids +soybean isoflavone group .Research kit was used to test the amounts of TG , HDL,LDL and IL-6,TNF-αin rats'blood.Bovine aortic endothelial cells were cultured and divided into control group,trans fatty acids group and trans fatty acids group +soybean isoflavone group .Western blot was performed to test the NF-κB phosphorylation ,as well as to determine ICAM-1 and VCAM-1 protein contents in bovine aortic endo-thelial cells.Results Compared with normal diet group ,the amounts of TG,LDL,IL-6,TNF-αincreased evidently (P<0.05), whereas the amounts of HDL decreased evidently (P<0.05) in the rats'blood of trans fatty acids group . After the soybean isoflavone intervened ,the amounts of TG,LDL,IL-6,TNF-αall decreased (P<0.01), meanwhile the amounts of HDL increased significantly (P<0.01).Soybean isoflavone inhibited NF-κB phosphorylation induced by trans fatty acids ( P<0.01) .The high expression of ICAM-1 and VCAM-1 induced by trans fatty acids was de-creased by soybean isoflavone when comparing with those in the control group ( P<0.01 ) .Conclusions Soybean isoflavone has a role in attenuating trans fatty acids trans fatty acids-induced atherosclerosis in rats .

Objective　To explore the metabolism of micronutri ents related to dark adaptation of radar operators through nutritional intervent ion. Methods　A total of 34 male radar operators aged between 18 ～29 years old were randomly divided into control and experimental groups. The c ontrol group were on normal diet, and the experimental group received the supple ment of VA, Zn and Se in additional to normal diet. The experiment lasted 4 week s. The levels of serum VA, Zn and Se were measured before and after the experime nt. Results　The levels of serum VA, Zn and Se in the experime ntal group were significantly higher than that in the control group after the experiment (P<0.01). Conclusion　The supplement of VA, Zn an d Se for 4 weeks may elevate, the levels of serum VA, Zn and Se significantly (reached or surpassed normal levels) and suggests that VA, Zn and Se su pplementation may effectively enhance the dark adaptation of radar operators.

[Objective]To evaluate the accuracy and value of CT guided biopsy of bone lesion.[Method]A total of 137 cases of CT guided biopsy were analyzed,including 1 case of cervical vertebrae,22 cases of thoracic vertebrae,47 cases of lumbar vertebra,8 cases of sacrum,9 cases of pelvis,2 cases of scapula and 48 cases of extremities.The biopsies were completed in local anesthesia under CT guidance,using trephine needle or Tru-cut needle.[Result]The puncture accuracy was 100%,the general diagnostic accuracy was 89.0%(122/137),in which the diagnostic accuracies of metastasis lesion,primary bone tumor and benign lesion were 95.8%(46/48),85.7%(36/42)and 85.1%(40/47)respectively.[Conclusion]CT guided biopsy was a safe and accurate method with little trauma and complication,having great value in deciding treatment of bone lesions.

Objective Through retrospective analysis of the thoracotomy of pulmonary metastases form osteogenic sarcomas in order to explore the related factors of prognosis and to improve the outcome of treatment.Methods From October1980to March2003,32cases with pulmonary metastases from sarcoma of bone were reviewed.62of thoractomy were performed for the patients,61of which were accomplished in the department of orthopaedics,and58operations were performed by the authors.There were32males and10females aging from11to61years with an average of 22.1years.The primary tumors of the patients were25oateosarcomas,3chondrosarcomas,2juxtacortical osteogenic sarcomas,and1each of fibrosarcoma and Ewings sarcoma.Of 32cases,21(65.6%)were treated with limb salvage,and11(34.4%)by am puta-tion.According to Enneking system,there wereⅡB in26andⅢB in5.25(78.1%)cases were bi lateral multiple metastases,6(18.8%)cases were unilateral solitary metastasis,and1(3.1%)case was unilateral mul tiple metastases.Most of the procedures of operation were local resection of the tumor ;however,only5cases underwent lobectomy.Results The postoperative complications included1case of severe bleeding cured by exploratory thoracotomy,2cases of lethal infections,and1case of atelectasis.Of 32cases,11sur -vived with out tumor at final follow-up.In25osteosarcoms,16were dead,2were lost from the follow-up;7cases of the other18cases that bilateral resections performed,were alive without tumor (38.9%)with a me di an survival time of 7years and3months.1each of Ewings sarcoma,paraosteal osteosarcoma and,chon-drosarco ma survived without tumor for 22years and5months,19years and3months,and13years and3months respectively.Conclusion Thoracotomy of pulmonary metastases of osteogenic sarcomas is an ef-fective method to salvage the patients life.The procedure of metastasectomy is local resection.The results al-so suggest that newadjurant chemotherapy is a important way to effect the condition of pulmonary metastases of osteogenic sarcomas.

[Objective]To evaluate the operability and clinical efficacy of injectable calcium sulfate for grafting bone defects.[Method]From June 2004 to August 2005,22 cases with benign bone lesions were treated with curettage and grafting with injectable calcium sulfate bone graft substitute(minimally invasive injectable graft X3,MIIG X3).There were 13 male cases and 9 femal cases.Their average age was 34.6(ranged,4~55).All the 22 cases were followed-up from 23 months to 37 months,average 31.6 months.All cases took radiographic examination regularly after the operation to assess the absorption rate of MIIGX3 and the formation rate of new bone.[Result]This injectable calcium sulfate with vacuum mixing was easy to use,providing complete fill and bony strength instantly when grafting bone defects.According to the raeiographs,MIIGX3 was absorbed almost at the same rate as new bone ingrowth in 8 to 24 weeks.[Conclusion]MIIGX3 is convenient to graft bone defects with fewer complications,capable of absorbing almost at the same rate as replacement of new bone.It's one of the best bone graft substitutes for grafting bone defects induced by benign bone lesions.

To observe the protective effect of echinacoside on mitochondrial fragmentation, mitophagy and cell apoptosis in human neuroblastoma SH-SY5Y cell line exposed to 1-methyl-4-phenylpyridinium (MPP(+)).

Objective To analyze the implementation effect of formative assessment in nutri-tion and food hygiene. Methods 55 students of Class One from grade 2010 majoring in preventive medicine were taken as the control group, with 56 students of Class Two as the experimental group.The control group used the summative assessment , while the experimental group used the formative assessment. Results were analyzed by course examinations and questionnaire surveys. SPSS 17.0 soft-ware was used and the measurement data were expressed by x±s. Statistical analysis was performed using t test, test, rank sum test, a Yuan linear correlation analysis, with the level of test α=0.05. Results The experimental scores and final scores of experimental group students were significantly higher than the control group and the difference was statistically significant(P values were 0.001, 0.000). The experimental group's overall result is closer to the negative skewed distribution. The ex-perimental group's average score of 89 points is significantly higher than the control group's average score of 79 points(P<0.01), and the usual results and the final results were positively correlated(r=0.865, P<0.001). The survey indicated that the students recognized the application of formative assess-ment in teaching process . Conclusion Formative assessment can help improve the teaching and learning quality in nutrition and food hygiene.

OBJECTIVE To explore cell death type of lateral line hair cellsinduced by cisplatin in zebrafish. METHODS Zebrafish larva were incubated in 1mM cisplatin solution for 6 hrs to induce about 90%lateral line hair cells loss. Time lapse imaging was used to detect the morphology of cisplatin-incubated hair cells in wildtypezebrafish pre-labelled by live dyes Bodipy TR C5-ceramide and Sytogreen 24. TUNEL assay and In situ anti-active Caspase-3 antibody staining were performed to detect nuclei fragmentation and Caspase-3 activity respectively. RESULTS Compared to control group, hair cells condensationand nuclei fragmentation (P<0.05) were detected in cisplatin-incubated group, and active Caspase-3 activity was also observed after cisplatin addition. CONCLUSION Cisplatinmay induced zebrafish lateral line hair cells loss by Caspase-3-dependent apoptotic pathway.

Objective To assess the clinical characteristics of systemic lupus erythematosus (SLE) in the elderly in Xinjiang.Methods As a retrospective study,clinical date and laboratory test results were collected from 87 elderly SLE patients and 222 non-elderly SLE patients (age ＜ 50 years) hospitalized in the People's Hospital of the Xinjiang Uygur Autonomous Region from January 2006 to July 2016.Results The female to maleratio was 5.7 ∶ 1.0 in the older-onset SLE group and the female to male ratio was 11.3 ∶ 1.0 in the younger-onset SLE group.Of the 13 patients with predisposing factors in the older-onset SLE group(14.9 ％),11 had infection (12.6 ％),whereas the 27 patients with predisposing factors in the younger-onset SLE group (12.2 ％)had pregnancy,abortion or insolation,in addition to infection.Arthritis (63.2 ％),weakness (40.2 ％),malar rashes (36.8 ％),anorexia(26.4 ％),and shortness of breath(26.4 ％) were common clinical manifestations in the olderonset SLE group.The incidence of anorexia in the older-onset SLE group was higher than that in the younger-onset SLE group(P＜0.01),while the incidence of trichomadesis was lower in the older-onset SLE group than that in the younger-onset SLE group(P＜0.01);Aminotransferase elevation,creatinine elevation and thrombocytopenia were more common in the older-onset SLE group than in the younger SLE group (P＜0.05).Lower rates of positive anti-Smanti body and anti-Acl antibody were found in the older onset SLE group,compared with the younger-onset SLE group (P＜0.05);Han older-onset SLE patients showed higher rates of oral ulcers than older-onset SLE patients of Uygur,Kazak or other ethnic minorities (P＜0.05).There were no significant differences between Han and Uygur/Kazak patients in laboratory test results.Conclusions The proportion of male SLE patients in the elderly is higher than that in non-elderly SLE patients in Xinjiang.Also,elderly SLE patients are prone to oral ulcers but often do not show typical early clinical symptoms and have low levels of specific antibodies.Therefore,clinicians should pay particular attention to older onset SLE patients in order to reduce the rates of misdiagnosis and missed diagnosis.

Objective To study the clinical application value of humidified high flow nasal cannula(HHFNC) on bronchitis in children.Methods Total 85 cases of bronchitis that needed oxygen therapy in our department from Oct 2015 to Feb 2016 were randomly divided into three groups,HHFNC group,NCPAP group and nasal cannula oxygen group(control group).According to the blood gas results,FiO2 was adjusted to maintain PaO2 in 50~70mmHg(1mmHg=0.133kPa),TcSO2 90% to 95%.The main symptoms and signs(wheezing,shortness of breath,three depression sign,wheezing rale) disappeared time,length of oxygen inhalation and stay,the change of PaO2,PaCO2,respiratory rate were compared among the three groups.Results Compared with control group,the clinical symptoms and signs disappeared time and length of oxygen inhalation and stay were significantly shorter in HHFNC group(P<0.05).The recovery of blood gas and respiratory rate in HHFNC group were better than those in control group(P<0.05).No statistically significant differences existed between HHFNC group and NCPAP group(P>0.05).Conclusion HHFNC can significantly improve the clinical symptoms,signs and blood gas results in children with bronchitis,reduce the length of oxygen inhalation and stay.HHFNC is an effective and well-tolerated treatment for bronchitis in children.