Objective:To analyze the clinical data of primary pulmonary hypertension in children and improve its clinical diagnosis and treatment.Methods:The clinical data of 6 patients with primary pulmonary hypertension were collected and analyzed.Clinical diagnosis was made by ways of electrocardiography,ultrasound,and X-ray.All of them were received catheterization examination and angiography.Results:Heart failures were found in all patients with PPH.Signs of pulmonary hypertension were found by accessory examination.No heart malformation was found by catheterization examination.Conclusion:There are all kinds of clinical manifestations of PPH.The definite diagnosis of PPH will rely on catheterization examination.

Objective:Analysis of complex congenital heart disease of children and the incidence of coronary abnormalities to explore the21(4):453-457. diagnostic value of coronary angiography.Methods:Angiocardio graphy cardiac catheterization of 229 children in Heart Center of Children's Hospital Chongqing Medical University from January 2006 to June 2009,the results were retrospectively analyzed,including the types and proportion of congenital heart diseases,incidences of coronary artery abnormalities,incidences of abnormal coronary artery crossing right ventricular outflow tract and prognosis.Results:Altogether,27(11.8%)cases of children with Congenital Coronary Artery Anomalies were detected.Highest incidenc(e6.55%)is L-coronary artery,which occupy 55.56% of Coronary Artery Anomalies.16(6.99%) cases of abnormal coronary artery crossing the right ventricular outflow tract,which including 7 cases of TOF,3 cases of DORV,3 cases of PA/VSD,1 case of TGA,1 case of CAVC and 1 case of SV/TGA.1 patient died of angiocardiography among the 27 cases of complicating coronary artery malformation,accounting for 3.7%in the total number of the angiography of complex congenital heart disease complicating coronary artery malformation,accounting for 0.44%in the total number of complex congenital heart disease.3 cases died after surgery, accounting for 16.7% of surgery.Conclusion:The incidence of coronary artery abnormalities in complex congenital heart disease in children is higher than the normal population;coronary angiography is an important means of final diagnosis coronary artery congenital variation.

Acupuncture Therapy ; Adult ; Diarrhea ; therapy ; Humans ; Male

China ; epidemiology ; Humans ; Metabolic Syndrome ; epidemiology ; prevention & control

The chemical constituents of starfish Asterias amurensis have been extensively studied in China. Some unique and new chemical constituents have been isolated from Asterias amurensis ; they included saponins, sulfated sterols, amyloses, steroids, amino acids, etc. Many of them have anti-bacterial activities and hemolysis effects. We reviewed the related literatures published home and abroad, hoping to provide more evidence for further development of Asterias amurensis.

Objective To investigate the inhibitory effect of genistein on the invasion of ovarian carcinoma cell line SKOV 3 in vivo and in vitro. Methods The abilities of the genistein-treated SKOV 3 cells to invade through reconstituted matrigel in transwell chambers were investigated in vitro and the invasion effect in vivo was determined using the xenograft models of SKOV 3 in nude mice. Results The ability of the 20 ?mol/L genistein-treated cells to invade the reconstituted basement membrane was decreased significantly at 72 h. This inhibition was in a dose-dependent manner. Genistein at the dose of 40 ?mol/L had the strongest effect. The results in vivo suggested that the grade of invasion in control SKOV 3 cells was in a time-dependent manner and genistein-treated group could apparently inhibit the progress of invasion, localizing the tumor in invasion grade 0 or grade I, and decreasing the proportion of grades Ⅱ, Ⅲ, Ⅳ. Conclusion The results suggest that genistein has inhibitory effect on the invasion of human ovarian carcinoma cell lines SKOV 3 in vivo and in vitro.

Objective To analyze and compare the HBV DNA contents in serum and breast milk after injection of hepatitis B im‐munoglobulin (HBIG) in different periods of pregnant and lying‐in women to provide the experimental basis for blocking the mater‐nal‐neonatal transmission(PMTCT) and breast feeding scheme .Methods 140 pregnant women carrying hepatitis B virus with HB‐sAg(+ ) by antenatal examination in the obstetric outpatient department of our hospital from June 2012 to June 2014 were selected and divided into the research group and the control group according to the voluntary and secretive principle .Among them ,75 cases in the research group were intramuscularly injected by high titer HBIG 200 U at 28 ,32 ,36 weeks of pregnancy ,while 65 cases in the control group were injected by HBIG at the end of pregnancy due to different causes .Serum HBV‐DNA content before injection and before delivery was detected in the two groups ,and which in neonatal serum and breast milk within 3-5 d also detected .The differences and correlation between the two groups were analyzed .Results The HBV‐DNA content <500 copies/mL ,500-1 × 106 copies/mL ,>1 × 106 copies/mL before HBIG injection in the research group were 28 cases ,17 cases ,30 cases respectively ,which before delivery were 35 cases ,20 cases ,20 cases respectively ;which in antenatal twice detection in the control group were 19 cases , 21 cases ,25 cases and 20 cases ,17 cases ,28 cases respectively ;neonatal serum HBV‐DNA positive in the research group and control group had 1 case(5 .3% ) and 5 cases (7 .7% ) respectively ;the breast milk HBV‐DNA positive in the two groups had 3 cases(4% )/and 8 cases(12 .3% ) respectively .Conclusion HBIG injection at late pregnancy in the pregnant women carrying HBV could influ‐ence the HBV replication ,thus reduces the probability of neonatal intrauterine infection ,at the same time reduces the HBV‐DNA positive rate of postpartum breast milk .

Objective To investigate how genistein to inhibit the invasion of human ovarian serous papillary cystadenocarcinoma cell line SKOV3.Methods Millicell chamber and coculture method were used to establish chemotactic migration model in vitro to observe the effect of genistein on directional chemotactic migration movement of SKOV3 cells.The protein expressions of cell surface adhesion molecule CD44v6,matrix metalloproteinases and tissue inhibitor of metalloproteinases MMP-9/TIMP-1 and MMP-2/TIMP-2 were determined by using immunocytochemical method and their mRNA levels were examined by in situ hybridization.Results Compared with control group,the directional chemotactic migration of SKOV3 were significantly decreased after treated with 20 ?mol/L and 40 ?mol/L genistein,reached to(46.9?5.8)% and(28.3?4.7)% respectively(P

Micrometastasis are frequently accompanied by the majority of cases of invasive malignant tumor, being a compacted relationship with the prognosis of patients. We should pay more attentions to the occurrence of micrometastasis in the diagnosis and management of carcinoma of urinary bladder, which is the most commonly diagnosed malignancy in urinary system and a significant cause of morbidity and mortality.This article introduces the diachronic concept of micrometastasis and the major methods for the detections of micrometastasis, moreover,it reviews recent advances about the detection of micrometastasis in bladder cancer, and about the benefits that patients of the disease might have received in the treatment of micrometastasis.

Angiogenesis plays an important role in solid tumors and hematologic malignancies. Angiopoietins act as essential regulators in this process,complementary with another mediator-VEGF. In the development of hematologic malignancies, the expression of Angs/Tie-2 system is identified abnormal, especially for Ang-2, whose expression and prognostic impact have gained significant attention recently.