No abstract available.
Depression* ; Humans ; Seoul*

In view of the potential of replicase protein as a diagnostic reagent for human caliciviruses (HuCVs), we have cloned and over-expressed this gene from the Snow Mountain-like Korean strains in Escherichia coli as a fusion protein with glutathione S-transferase (GST, and described the preliminary antigenic characterization of the recombinant products. Each 470bp fragment corresponding to highly conserved region of RNA-dependent RNA polymerase was generated by RT-PCR from stools of two diarrheal children, cloned in pMOSBlue T-vector, and subcloned between the EcoRI and SalI restriction sites of pGEX-47-3, a GST gene fusion vector, yielding pGCVpol. This construct expressed a Snow Mountain-like HuCV replicate under the control of the IPTG-inducible pac promoter. An extract prepared by sonication of the E. coli cell inclusion bodies bearing pGCVpol products was purified and analyzed by SDS-PAGE. After Coomassie blue staining, it was shown that the recombinant replicase migrated on the gels with an approximate molecular mass of 46.5 kDa, that was subsequently cleaved into a 26 kDa GST fragment and a 20.5 kDa replicase protein upon digestion with thrombin protease. The replicase was recognized on immunoblotting with the sera from symptomatic children with the HuCV-associated diarrhea but not by asymptomatic sera from adults. The results presented the first biological activity of individually expressed HuCV replicase subunit and provided important reagents for diagnosis of HuCV infection.
Adult ; Child ; Clone Cells ; Diagnosis ; Diarrhea ; Digestion ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; Gels ; Gene Fusion ; Glutathione Transferase ; Humans ; Immunoblotting ; Inclusion Bodies ; Indicators and Reagents ; RNA Replicase ; Snow* ; Sonication ; Staphylococcal Protein A ; Thrombin

With simple abdomen, double contrast colon study, and ultrasongraphy, authors retrospectively analyzedradilogic findings of 46 cases which were proved periappendiceal abscess, due to perforation of appendix, byhistology or follow-up ultrasnongraphy from May in 1983 to July in 1985 at Yeung-Nam University Hospital. Theresuls obtained were as follows: 1. Of 46 cases, 24 cases were males and 22 cases females, with the results ofabove statistics, periappendiceal abscess was not influenced by sex. 2. Periappendiceal abscess was mostfrequently demonstrated in forties. 3. Comparison of ultrasonography and B.E. Pseudorenal pattern(Ultrasonography)- well defined defect (B.E.) Cystic pattern(Ultrasonography)- serrated defect (B.E.) 4. Missed 2 cases inultrasonography were detected in B.E., i.e. sensitivity of B.E. is higher than that of ultrasonography. 5.Differences of ultrasonography and B.E.
Abdomen ; Abscess ; Appendix ; Colon ; Female ; Follow-Up Studies ; Humans ; Male ; Retrospective Studies ; Sensitivity and Specificity ; Ultrasonography

Poor R-Wave Progression(PRWP) of precordial leads is frequently encountered electrocardiographic findings of uncertain significance and has simply been deemed as suggestion of anterior myocardial infarction without concrete ground. 217 cases with poor R-Wave Progression have been analyzed on clinical records and results are as follows. 1) PRWP was most frequently found in fifties and sixities, comprising 63.9% of the subjects. 2) Co-existent disease entities with PRWP were classified into three categories, cardiovascular diseases, chronic lung diseases and normal variants. 3) The cardiovascular diseases related with PRWP were mainly hypertensive diseases, comprising 59.8% of cardiovascular diseases, followed by ischemic heart disease, valvular heart disease and cardiomyopathies. 4) PRWP may be an early sign of acute myocardial infarction in a certain part of cases, which was endorsed by typical clinical symptoms and enzyme studies. 5) As the criterion of PRWP, V3R equal to or less than 3 mm was thought more adequate for higher specificity rather than 4 mm.
Cardiomyopathies ; Cardiovascular Diseases ; Electrocardiography ; Heart Valve Diseases ; Lung Diseases ; Myocardial Infarction ; Myocardial Ischemia ; Sensitivity and Specificity

No abstract available.
Plants, Medicinal*

Purpose: The study aimed to identify the effects of sleep hygiene (use of caffeine, alcohol, night eating syndrome, stress, and coping styles), social network, and smartphone-related factors on quality of sleep in young adults. Methods: This was a descriptive research design. Participants completed a questionnaire on evidence-based variables including caffeine intake, alcohol consumption, social network, night eating syndrome, stress, coping styles, and smartphone-related factors. Stepwise multiple regression was used for data analysis to identify factors that influenced the participants’ quality of sleep. This study included 288 young adults in South Korea. Results: This study identified the factors affecting quality of sleep in young adults. Their average weekly sleep duration was 6.86 hours with low sleep quality, indicated by a score of 59.34 points (range 17-100). The predictors of sleep quality were sleep mood, sub-items of night eating syndrome, effects of pain over the last four weeks, and social networks, which explained 33% of the variance. Conclusion Sleep-induced diseases in young adults could be prevented by identifying sleep mood, pain, and social networks, which is important for health and using them as a basis for intervention.

PURPOSE: To evaluate value of post-contrast 3-Dimensional fat suppressed Spoiled GRASS (FS SPGR) in detectiong subtle bony erosion and tenosynovitis of hands and wrists due to early rheumatoid arthritis. MATERIALS AND METHODS: Fourteen MR iraagings of the hands and wrists were performed in 7 early rheu- matoid arthritis without any abnormalities in plain radiography and in 7 healthy volunteers. All subjects underwent MR sequence of coronal 3D FS SPGR with and without contrast enhancornear in 1.5T MR unit. We evaluated the number of the bony erosion and tenosynovitis respectively in pre- and post-contrast FS SPGR images. RESULTS: The abnormal enhancing areas were not demonstrated in 7 healthy volunteers. Seven patients had 25 bony erosions in pre-contrast FS SPGR and 52 bony erosions with tenosynovitis (n=10) in post-contrast FS SPGR. Enhancing joint spaces were shown in 8 cases CONCLUSION: Post-contrast FS SPGR was better than pre-contrast FS SPGR in the evaluation of early rheumaotid arthritis and is valuable as a baseline study.
Arthritis ; Arthritis, Rheumatoid* ; Hand ; Healthy Volunteers ; Humans ; Joints ; Magnetic Resonance Imaging* ; Poaceae ; Radiography ; Tenosynovitis ; Wrist

PURPOSE: To determine whether triple negative (TN) early stage breast cancers have poorer survival rates compared with other molecular types. MATERIALS AND METHODS: Between August 2000 and July 2006, patients diagnosed with stage I, II early stage breast cancers, in whom all three markers (estrogen receptor, progesterone receptor, and human epidermal growth factor receptor [HER]-2) were available and treated with modified radical mastectomy or breast conserving surgery followed by radiotherapy, were retrospectively reviewed. RESULTS: Of 446 patients, 94 (21.1%) were classified as TN, 57 (12.8%) as HER-2 type, and 295 (66.1%) as luminal. TN was more frequently associated with young patients younger than 35 years old (p = 0.002), higher histologic grade (p < 0.0001), and nuclear (p < 0.0001). The median follow-up period was 78 months (range, 4 to 130 months). There were 9 local relapses (2.0%), 15 nodal (3.4%), 40 distant metastases (9.0%), and 33 deaths (7.4%) for all patients. The rates of 5-year OS, DFS, LFS, and DMFS for all patients were 95.5%, 89.9%, 95.4%, and 91.7%, respectively. There were no significant differences in OS, DFS, LFS, and DMFS between triple negative and other subtypes (p > 0.05). CONCLUSION: We found that patients with TN early stage breast cancers had no difference in survival rates compared with other molecular subtypes. Prospective study in homogeneous treatment group will need for a prognosis of TN early stage breast cancer.
Breast ; Breast Neoplasms ; Disease-Free Survival ; Follow-Up Studies ; Humans ; Mastectomy, Modified Radical ; Mastectomy, Segmental ; Neoplasm Metastasis ; Phenobarbital ; Prognosis ; Receptor, Epidermal Growth Factor ; Receptors, Progesterone ; Recurrence ; Retrospective Studies ; Survival Rate ; Treatment Outcome

Gastrointestinal cytomegalovirus(CMV) infection in adults is observed as a part of a generalized or localized infection in patients who are immunocompromized. We report a case of CMV infection of the small intestine. The patient is a 34 year-old woman who has complained of palpable purpura in the lower extremities and buttocks, and arthralgia of large joints. The skin biopsy showed fibrinoid necrosis and neutrophils with leukocytoclasis, which findings are compatible with hypersensitivity angiitis. The patient received steroid and cyclophosphamide. During the follow-up period, generalized edema and bloody stool were detected. Resected specimen of small bowel has multiple aphthous ulcer. Microscopically, cytomegalic cells are observed along the endothelial cells and mesenchymal cells. In situ hybridization using DNA probes against CMV revealed positive staining in the cytomegalic inclusions in vascular endothelial and mesenchymal cells.
Adult ; Male ; Female ; Humans ; Biopsy

PURPOSE: MgSO4 was used to evaluate the clinical effects in the treatment of severe persistent pulmonary hypertension of the newborn(PPHN). METHODS: Seven newborn infants with severe PPHN, and a persistent elevation of AaDO2(Alveolar-arterial oxygen tension difference) above 610 mmHg for more than 8 hours despite the aggressive conventional therapy were included. A loading dose of 200 mg/kg MgSO4 was given intravenously over 20-30 minutes, followed by continuous infusion of 20-50 mg/kg/hour. Vital signs, arterial blood gases, serum electrolytes, calcium and magnesium, and ventilatory setting were measured and recorded before MgSO4 was given and periodically thereafter. Severity of impairment of gas exchange was evaluated by AaDO2 and oxygenation index(OI). RESULTS: Mean gestational age and birth weight of seven infants were 38+5 weeks and 2,949844 grams, and one of them was a premature infant with gestational age of 27 weeks, and birth weight of 1,096 grams. There were six males and one female. Four infants were outborn. Four infants were presented with meconium aspiration syndrome, one with birth asphyxia, one with respiratory distress syndrome, and one with subdural hemorrhage. Before the start of the MgSO4 treatment, the mean AaDO2 and OI of seven infants were 638.8+/-10.8 mmHg and 43.811.2, respectively. During the treatment AaDO2 and OI significantly decreased to 538.4+/-114.5 mmHg and 13.4+/-9.4 within 24 hours(P< 0.05). Hypermagnesemia was noted in 7 infants, hypocalcemia in three, bradycardia in five, and hypotension in two. Only one infant was discharged against medical advice, and remaining six survived. CONCLUSION: MgSO4 may be considered as an alternative treatment of severe PPHN when other conventional treatments fail, are contraindicated, or not available.
Asphyxia ; Birth Weight ; Bradycardia ; Calcium ; Electrolytes ; Female ; Gases ; Gestational Age ; Hematoma, Subdural ; Humans ; Hypertension, Pulmonary* ; Hypocalcemia ; Hypotension ; Infant ; Infant, Newborn* ; Infant, Premature ; Magnesium* ; Male ; Meconium Aspiration Syndrome ; Oxygen ; Parturition ; Vital Signs