OBJECTIVES: To compared the effect of different levels of moisture of root canal on the sealing ability after filling with four different types of sealer. MATERIALS AND METHODS: Single-rooted teeth (n = 90) instrumented to and apical size of 0.06 / 45 were randomly assigned to 12 experimental groups (n = 7 per group), positive/negative control groups (n = 3 per group). The teeth of the experimental groups (a. DRY; b. PAPER POINT DRY; c. WET) were obturated with sealer (Group 1-3: Sealapex; Group 4-6: AH plus; Group 7-9: Tubuli-seal; Group 10-12: EndoRez) and warm vertical compaction method. After 7 days in 37degrees C, 100% humidity, the coronal-to-apical microleakage was evaluated quantitatively using a glucose leakage model. The leaked glucose concentration was measured with spectrophotometer at 1, 3, 7, 14, 21, and 30 days. Data were recorded ad mmol/L and statistically analysed with the two-way ANOVA and Duncan test (p = 0.05). RESULTS: Throughout the experimental period Tubuli-seal/WET (Group 9) showed the highest mean cumulative glucose penetration (178.75 mmol/L), whereas AH plus/DRY (Group 4) had the least (20.78 mmol/L). CONCLUSIONS: The results of this study demonstrated that the moisture condition of root canals at the time of obturation and the type of sealer that was used had a significant effect on leakage and sealing ability. Thus drying procedure according to sealer types is a critical step and should not be missed in endodontic treatment.
Calcium Hydroxide ; Composite Resins ; Dental Pulp Cavity ; Epoxy Resins ; Glucose ; Humidity ; Root Canal Filling Materials ; Salicylates ; Tooth

The purpose of this study was to investigate the bonding of resin-based root canal sealer, AH26 when the sealer was applied as a thin layer between dentine and gutta-percha surface. In this study, forty non-caries extracted human molars and resin-based root canal sealer(AH 26, DeTrey/Dentsply, Germany) were used. Disks of gutta-percha, 6mm in diameter.6mm thick (Diadent/Dentsply, Korea) for thermoplastic obturation were used and dentin surfaces were treated with 2% NaOCl(Group 1) or 2%NaOCl+17% EDTA(Group 3). Disks of gutta-percha, 6mm in diameter.6mm thick (Diadent/Dentsply, Korea) for conventional obturation were used and dentin surface were treated with 2% NaOCl(Group 2) or 2%NaOCl+17% EDTA(Group 4). Enamel was removed by a horizontal section 1mm below the deepest portion of the central occlusal groove by using a water-cooled low speed diamond saw. A second horizontal section was done around cementoenamel junction. Exposed dentin surface was cut to approximately 8x8 mm rectangular shape and was ground against 320, 400, 600 grade silicon carbide abrasive paper serially. After grinding, the dentine surface were soaked in a solution of 2% NaOCl for 30 minutes and twenty of specimens were treated with 17% EDTA solution for 1 minute. The treated specimens were washed and dried. Root canal sealer, AH26 was prepared according to the manufacture's instructions. The Gutta-percha and dentin surface were coated with a thin layer of the freshly mixed sealer. The specimens were left overnight at room temperature. After their initial set, they were transferred to an incubator at 37degrees C for 72 h. After 72 hours, resin blocks were made. The resin block was serially sectioned vertically into stick of 1.1mm. Twenty sticks were prepared from each group. After that, tensile bond strength for each stick was measured with Microtensile Tester. Failure patterns of the specimens at the interface between gutta-percha and dentin were observed under the SEM(x1000) and Stereomicroscope (LEICA M420, Meyer Inst., TX U.S.A) at 1.25 x25 magnification. The results were statistically analysed by using a One-way ANOVA and Tukey's test. The results were as follows; 1. Tensile bond strengths(mean+/-SD) were expressed with ascending order as follows: Group 1, 3.09+/-1.05MPa ; Group 2, 6.23+/-1.16MPa ; Group 3, 7.12+/-1.07MPa ; Group 4, 10.32+/-2.06MPa. 2. Tensile bond strengths of the group 2 and 4 used disks of gutta-percha for conventional obturation were significantly higher than that of the group 1 and 3 used for thermoplastic obturation. (p < 0.05) 3. Tensile bond strengths of the group 3 and 4 treated with 2% NaOCl+17% EDTA were significantly higher than that of the group 1 and 2 treated with 2% NaOCl. (p < 0.05) 4. In analysis of failure patterns at the interface between sealer and gutta-percha, there were observed 49 (61%)cases of adhesive failure patterns and 31(39%) cases of mixed failures patterns.
Adhesives ; Dental Enamel ; Dental Pulp Cavity* ; Dentin* ; Diamond ; Edetic Acid ; Gutta-Percha* ; Humans ; Incubators ; Molar ; Tooth Cervix